==== Front Behav Neurol Behav Neurol BN Behavioural Neurology 0953-4180 1875-8584 Hindawi 10.1155/2020/1719360 Research Article Clinical and Imaging Prognosis in Patients with Delayed Encephalopathy after Acute Carbon Monoxide Poisoning https://orcid.org/0000-0001-8366-4571Liu Jinzhi drjinzhiliu@163.com 1 2 Si Zhihua 1 2 Liu Jie 1 2 Lin Yan 1 2 Yuan Jing 1 2 Xu Shan 1 2 He Yan 1 2 Zhang Tao 1 2 https://orcid.org/0000-0001-5595-5961Wang Aihua draihuawang@163.com 1 2 1Department of Neurology, Shandong Provincial Qianfoshan Hospital, The First Affiliated Hospital of Shandong First Medical University, Jinan, China 2Department of Neurology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, China Academic Editor: Nicola Tambasco 2020 7 12 2020 2020 17193602 7 2020 9 9 2020 31 10 2020 Copyright © 2020 Jinzhi Liu et al.2020This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Objective To study the clinical manifestations, magnetic resonance imaging (MRI) findings, and prognosis of delayed encephalopathy after carbon monoxide poisoning (DEACMP). Methods The medical records of 20 patients with DEACMP were retrospectively reviewed. All the patients received hyperbaric oxygen treatment and other treatments as necessary. Results The patients had diverse clinical manifestations, including memory deficits, personality changes, cognitive or executive function deficits, mood disorders, Parkinsonism, dystonia or other motor impairments, and akinetic mutism. MRI revealed lesions in the bilateral cerebral white matter and/or basal ganglia. Except for the pathologically confirmed DEACMP, epileptic seizure, hemiplegia, and vegetative state, the remaining symptoms had been improved, especially the cognitive impairment, which had been decreased from 95% to 25% and psychiatric symptoms also decreased from 95% to 55% at the 6-month follow-up. Conclusions The prognosis of patients with DEACMP was poor, and they had a relatively severe disability. The early use of hyperbaric oxygen is of great significance to improve clinical efficacy and get a better prognosis. key research and development plan of Shandong Province2019GSF1080082019GSF108033National Natural Science Foundation of ChinaQYPY2019NSFC0616Key Science and Technology Program of Jinan City201704113Natural Science Foundation of Shandong ProvinceZR2016HP23ZR2019MH062Traditional Chinese Medicine Science and Technology Development Program of Shandong Province2017-1632019-0369 ==== Body 1. Introduction Carbon monoxide (CO) is a type of colorless, tasteless, odorless, and nonirritating gas with toxicity, resulting from the incomplete combustion of carbon-based fuels [1]. CO poisoning is a leading contributor to deaths from poisoning worldwide, including the United States, China, Japan, and Europe, Turkey [2–6]. Global epidemiologic data reveal that the accumulative morbidity and mortality of CO poisoning are approximately 137 cases and 4.6 deaths per million throughout the world [7]. Furthermore, it has been reported that the incidence of CO poisoning is higher in developing countries like China than in developed countries such as the United States due to the wide application of nonclean energy [8]. Of note, CO poisoning is the most common poisoning in northern China, as most people rely on coal burning for heat in the cold season [9]. The mechanism underlying CO poisoning is that the affinity of CO and hemoglobin (Hb) is 200-300 times stronger than that of oxygen and hemoglobin, resulting in systemic tissue hypoxia [10]. Studies have shown that hypoxia-caused CO in the brain produced too many oxygen free radicals, which easily damaged the nerve cells, leading to CO poisoning symptoms. Delayed encephalopathy after carbon monoxide poisoning (DEACMP) is a group of neuropsychological disorders that can occur days or weeks after the cessation of symptoms of acute CO poisoning [11–13]. Previous studies have indicated that 0.2%-40% of CO poisoning patients develop DEACMP. Serious DEACMP is usually fatal and as many as 50% of DEACMP individuals develop cognitive, neurological, or neurobehavioral sequelae [14]. After the false recovery periods, patients with acute CO poisoning symptoms reappear including neurological symptoms, cognitive disorders, mental disorders, and pyramidal or extrapyramidal symptoms. Common neurological manifestations of DEACMP include disorders such as computational power and memory loss, linguistic disorders, dystonia, orientation disorders, dementia, Parkinson's syndrome, and kinetic mutations [15]. The changes of CT and MRI were consistent with the pathological basis. The abnormality rate of CT in the brain was about 87.5%, which was manifested as the diffuse abnormal signal changes of the bilateral globus palidus, forehead, dorsal occipital brain hemisphere white matter and bilateral basal nuclear region low-density changes, lateral ventricle expansion, sulcus widening, and brain tissue atrophy. The main performance of MRI was shown as follows: (1) subcortical white matter abnormalities: mainly under the cortical point, patchy or fusion-like T1WI low signal and T2WI high signal; (2) deep white matter lesions: the periventricular white matter and semioval center, the more extensive T2WI low signal and T2WI high signal; and (3) basal ganglia lesions: bilateral white-white symmetrical T1WI low signal and T2WI high signal [12, 16–18]. To better understand the clinical course of DEACMP and lessen the associated disability, this study investigated the clinical characteristics, MRI findings, and prognosis of 20 patients with DEACMP. 2. Materials and Methods 2.1. Patients This retrospective study was approved by the Ethics Committee of Shandong Provincial Qianfoshan Hospital, China (NO. EAFQ2012022). Written informed consent was obtained from all the patients or their relatives between January 2012 and January 2013. From January 2010 to January 2013, 100 patients with toxicity were admitted to the emergency department of our hospital. Among these, 20 patients, 12 males and 8 females, with the mean age of 61 years fulfilled the following inclusion criteria: (1) a clear history of CO poisoning, resulting in a coma, in the previous 1-2 months; (2) normal or nearly normal performance in the interim period (i.e., between recovering from the immediate coma caused by CO poisoning and the occurrence of clinical manifestations of DEACMP); (3) clinical features of acute dementia or full-brain damage, including advanced neurological deficits, psychiatric symptoms, pyramidal system disorders, and extrapyramidal manifestations, coupled with peripheral and cranial nerve damage; and (4) no other intracranial lesions. MRI was performed on the first day of hospitalization using a 3-T magnet (GE Signa HDx 3 T) [12]. T1-weighted images (repetition time, 1770 ms; echo time, 9.0 ms), T2-weighted images (repetition time, 4400 ms; echo time, 100 ms), and T2-weighted FLAIR images (repetition time, 7000 ms; echo time, 86 ms) were obtained in the coronal, sagittal, and axial planes. Only patients with prominent leukoencephalopathy but no subclinical leukoencephalopathy were recruited. 2.2. Classification of cognitive impairment Cognitive performance and functional state were assessed before and after treatment by the neuropsychologists. The classification is as follows: Mild: mild injury of brain tissue, decline of computing power, memory and understanding ability, abnormal language and action, and poor self-care ability Moderate: in addition to mild performances, heavier injuries of the central nervous system or other vital organs and tissues, and incoherent speech, sudden abuse, shouting, severe depression, hemiplegia, urinary incontinence, and other symptoms Severe: severe damage of the central nervous system, the patient presented with a coma or even persistent vegetative state 2.3. Treatment All the patients underwent at least one session of hyperbaric oxygen therapy (2.5-3.0 absolute atmospheres (atm) for ≥90 min). The pressure was increased from 1 atm to 2.8 atm over the first 18 min, maintained at 2.8 atm for 50 min, and then decreased to 1 atm over 22 min. This procedure was carried out five times a week. Supportive therapy was provided when necessary, including the administration of edaravone, a free radical scavenger, and selegiline, a monoamine oxidase inhibitor. The period of hospitalization ranged from 20 to 30 days, depending on the clinical condition of the patient. 3. Results 3.1. Clinical Characteristics and Follow-Up Prognosis The main clinical characteristics were shown in Table 1. Nineteen patients (95%) exhibited a cognitive impairment before clinical treatments. Based on the clinical classifications, the condition of DEACMP patients was assessed as slight (3 patients), moderate (9 patients), and severe (7 patients). Three patients developed generalized tonic-clonic seizures, and one patient developed simple partial seizure (Table 1). After 6 months of follow-up, we found that except for the pathologically confirmed DEACMP, epileptic seizure, hemiplegia, and vegetative state, the remaining symptoms had been improved in all patients (Table 1). Among them, the cognitive impairment decreased from 95% to 65% (n = 2 slight, n = 7 moderate, and n = 4 severe) at 1-month follow-up, to 40% (n = 2 slight, n = 4 moderate, and n = 2 severe) at 3-month follow-up, and to 25% (n = 3 slight, n = 2 moderate, and n = 0 severe) at 6-month follow-up. In addition, psychiatric symptoms also decreased from 95% to 90% at 1-month follow-up, to 80% at 3-month follow-up, and to 55% at 6-month follow-up. 3.2. Imaging Findings The T2-weighted images and T2-weighted FLAIR images indicated high signal intensity in the bilateral cerebral white matter and bilateral or unilateral globus pallidus and periventricular white matter (Figure 1). There were 7 patients (35%) with high signal intensity in the bilateral cerebral white matter, 2 cases (10%) displaying basal ganglion lesions, and 11 patients (55%) with basal ganglion lesions accompanied by the periventricular white matter and semielliptical central lesions. 4. Discussion Two courses generally following exposure to CO are acute poisoning that occurs immediately after exposure to CO or acute poisoning complicated by delayed encephalopathy following a symptom-free interval of a few weeks or more [14]. Our data revealed that the occurrence of DEACMP accounted for about 20% in patients who underwent CO poisoning caused by unintentional accidents, which was consistent with previous studies [19, 20]. In this study, we reported the clinical characteristics of 20 patients with DEACMP and their associated neuroradiological changes. Before clinical treatment, 95% of the patients suffered from cognitive impairment and 100% had functional impairment. Except for the pathologically confirmed DEACMP, epileptic seizure, hemiplegia, and vegetative state, the remaining symptoms had been improved, especially the cognitive impairment had been decreased from 95% to 25% and psychiatric symptoms also reduced from 95% to 55% at the 6-month follow-up. Although the pathogenesis of DEACMP is not clear, it is generally believed that (1) vascular wall degeneration, vascular motor nerve palsy, vasodilation, congestion, vascular rupture, and occlusive arterial endocarditis can result in brain cell necrosis, softening, and degeneration; (2) oligodendrocytes are vulnerable to hypoxia, thereby triggering extensive demyelination, which is important for the disease progression; (3) immune dysfunction, abnormalities of dopamine, serotonin, acetylcholine, and other neurotransmitters and free radical-induced lipid peroxidation are involved in the occurrence and progress of the disease [21–24]. Hyperbaric oxygen treatment, as the most effective clinical therapy, of acute toxicity of CO poisoning, has a long history, which can significantly improve the prognosis of patients and reduce the incidence of DEACMP. Hyperbaric oxygen therapy can increase the physical dissolved oxygen content for the body to provide adequate oxygen metabolism without relying on HbO2 dissociation. With the increase of the oxygen supply, the vascular wall permeability can come back to normal, which may reduce oozing, cerebral edema, and intracranial pressure; promote the establishment of collateral circulation, and reduce anaerobic glycolysis, blood viscosity, and platelet aggregation. The incidence of DEACMP is usually around 12%-13% in patients who have been poisoned for more than 6 hours or who have not received hyperbaric oxygen therapy. In patients receiving hyperbaric oxygen therapy within 6 hours following poisoning, the incidence rate is approximately 1% [14, 25, 26]. However, little research has been reported about the use of hyperbaric oxygen therapy for DEACMP. Several studies suggest that the duration of coma, COHb saturation, and hyperbaric oxygen may be the risk factors. Besides, it has been demonstrated that there is a certain correlation between the clinical symptoms of DEACMP and the imaging characteristics [1, 11, 17]. When the cortical white matter shows abnormal signals, the clinical manifestations may include cognitive dysfunction and language disorders, accompanied by anxiety, depression, and other psychiatric symptoms. And the imaging of the basal ganglia lesions or basal ganglia with periventricular white matter and semioval center lesions may be not only associated with the cognitive dysfunction but also correlated with the accompanied nonvascular disease, dystonia, and other Parkinson's syndrome. DEACMP occurs in the white matter of the brain, causing demyelination [12, 17, 24]. We also deem that the cerebral hypoxia can cause the secondary microvascular injury, cell swelling, and degeneration; deep or intravenous congestion of the microvascular wall; and vascular occlusion, ultimately leading to brain cell necrosis, white matter loose, etc. Our study suggests that the treatment of DEACMP with hyperbaric oxygen therapy combined with hormones, circulations, and blood circulation therapy as soon as possible may improve the symptoms of the poisoning based on the diagnosis. It is worth noting that, in our study, there are more patients with basal ganglion lesions accompanied by the periventricular white matter and semielliptical central lesions when compared to those with simple basal ganglia lesions, which is related to the severity of the disease in the cases received by our hospital. Meanwhile, patients with simple basal ganglia lesions mainly present dystonia and Parkinson-like symptoms, which is associated with the low rate of consultation and hospitalization. It is widely acknowledged that the efficacy of hyperbaric oxygen may be related to the age, the disease classification, and the treatment [20, 21, 27]. Even so, we recommend that the patients should be actively applied with hyperbaric oxygen treatment regardless of the poisoning phase and they should not give up the hyperbaric oxygen therapy until the real brain death is diagnosed. In fact, some patients with the cognitive, neurological, or neurobehavioral sequelae may return to normal function after DEACMP for a long time. A prospective study is needed to analyze the long-term prognosis of the DEACMP patients. 5. Conclusions DEACMP patients have severe neurological damage and poor prognosis. From this study, we consider that the early use of hyperbaric oxygen is of great significance to improve clinical efficacy and get a better prognosis according to the analysis of high-risk factors combined with clinical symptoms and related checks. It is vital for individuals to take precautions to minimize potential exposure to carbon monoxide in the home. Acknowledgments This study was supported by grants from the Traditional Chinese Medicine Science and Technology Development Program of Shandong Province, China (2017-163, 2019-0369); Natural Science Foundation of Shandong Province, China (ZR2016HP23, ZR2019MH062); Key Science and Technology Program of Jinan City, China (201704113); Cultivation Fund for the National Natural Science Foundation of China of Shandong Provincial Qianfoshan Hospital (QYPY2019NSFC0616); and key research and development plan of Shandong Province, China (2019GSF108008, 2019GSF108033). Data Availability The analyzed data sets generated during the study are available from the corresponding author on reasonable request. Conflicts of Interest The authors declare that there is no conflict of interest regarding the publication of this article. Figure 1 magnetic resonance imaging (MRI) scan showed high signal intensity in the bilateral cerebral white matter (a, b) and the globus pallidus(c, d). Table 1 Clinical characteristics and follow-up prognosis. Symptoms Cases Follow-up 1 m Follow-up 3 m Follow-up 6 m Cognitive impairment 19 (95%) 13 (65%) 8 (40%) 5 (25%) Urinary incontinence 14 (70%) 14 (70%) 12 (60%) 11 (55%) Psychiatric symptoms 19 (95%) 18 (90%) 16 (80%) 11 (55%) Pathologically confirmed DEACMP 6 (30%) 6 (30%) 6 (30%) 6 (30%) Extrapyramidal symptoms 18 (90%) 18 (90%) 17 (85%) 15 (75%) Mutism 18 (90%) 18 (90%) 17 (85%) 16 (80%) Epileptic seizure 4 (20%) 4 (20%) 4 (20%) 4 (20%) Pseudobulbar palsy 16 (80%) 16 (80%) 15 (75%) 12 (60%) Hemiplegia 3 (15%) 3 (15%) 3 (15%) 3 (15%) Vegetative state 11 (55%) 11 (55%) 11 (55%) 11 (55%) The data were shown as n (percentage). DEACMP: delayed encephalopathy after carbon monoxide poisoning. ==== Refs 1 Inagaki T. Ishino H. Seno H. Umegae N. Aoyama T. A long-term follow-up study of serial magnetic resonance images in patients with delayed encephalopathy after acute carbon monoxide poisoning Psychiatry and Clinical Neurosciences 1997 51 6 421 424 10.1111/j.1440-1819.1997.tb02611.x 9472130 2 Ruth-Sahd L. A. Zulkosky K. Fetter M. E. Carbon monoxide poisoning: case studies and review Dimensions of Critical Care Nursing 2011 30 6 303 314 10.1097/DCC.0b013e31822fb017 2-s2.0-80054049464 21983502 3 Chen F. Ye Y. Wei Q. Non-fire related carbon monoxide poisoning in Sichuan, China: a 9-year study (2008-2016) Iranian Journal of Public Health 2019 48 3 458 464 31223573 4 Kinoshita H. Türkan H. Vucinic S. Carbon monoxide poisoning Toxicology Reports 2020 7 169 173 10.1016/j.toxrep.2020.01.005 32015960 5 Dirlik M. Bostancıoğlu B. Deaths due to carbon monoxide poisoning in Aydın, western Turkey Death Studies 2016 41 4 246 250 10.1080/07481187.2016.1259693 2-s2.0-85007332225 27849445 6 Braubach M. Algoet A. Beaton M. Lauriou S. Héroux M. E. Krzyzanowski M. Mortality associated with exposure to carbon monoxide in WHO European member states Indoor Air 2013 23 2 115 125 10.1111/ina.12007 2-s2.0-84875052216 23025441 7 Mattiuzzi C. Lippi G. Worldwide epidemiology of carbon monoxide poisoning Human & Experimental Toxicology 2020 39 4 387 392 10.1177/0960327119891214 31789062 8 Xue J. Sun Q. Wang Y. Gao G. Shi L. Yu T. Features of carbon monoxide poisoning in China Iranian Journal of Public Health 2013 42 10 1192 1193 26060630 9 Zhang Y. Yu B. Wang N. Li T. Acute poisoning in Shenyang, China: a retrospective and descriptive study from 2012 to 2016 BMJ Open 2018 8 8, article e021881 10.1136/bmjopen-2018-021881 2-s2.0-85052741198 30158226 10 Kwon O. Y. Chung S. P. Ha Y. R. Yoo I. S. Kim S. W. Delayed postanoxic encephalopathy after carbon monoxide poisoning Emergency Medicine Journal 2004 21 2 250 251 10.1136/emj.2002.002014 2-s2.0-1542710314 14988368 11 Gu R. J. Li W. Q. Wang J. K. Clinical significance of 5-HT and DA levels in serum and cerebrospinal fluid of the patients with delayed encephalopathy after acute carbon monoxide poisoning Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2011 29 2 121 124 21619841 12 Wang X. Li Z. Berglass J. MRI and clinical manifestations of delayed encephalopathy after carbon monoxide poisoning Pakistan Journal of Pharmaceutical Sciences 2016 29 6 Suppl 2317 2320 28167472 13 Yu H. Pan X. Meng J. Ding J. Xu G. Fang G. A clinical study on delayed encephalopathy after carbon monoxide poisoning Zhonghua lao dong wei sheng zhi ye bing za zhi= Zhonghua laodong weisheng zhiyebing zazhi= Chinese Journal of Industrial Hygiene and Occupational Diseases 2002 20 1 26 28 14694610 14 Wang W. Z. Qi H. N. Xiao Q. M. Effects of Ginaton on nitric oxide and nitric oxide synthase in patients with delayed encephalopathy after carbon monoxide poisoning Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2017 35 1 30 33 10.3760/cma.j.issn.1001-9391.2017.01.007 28241699 15 Gu R. J. Wang X. H. Zhang P. Change of the serum interleukin 6 in patients with delayed encephalopathy after acute carbon monoxide poisoning Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2005 23 6 461 462 16405788 16 Chen Z. Q. Yang W. J. Cai L. Clinical characteristics, CT and MRI findings for delayed encephalopathy after acute carbon monoxide poisoning Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2005 23 6 438 441 16405778 17 Liu W. P. Xiao B. Zhang J. Study of delayed encephalopathy after acute carbon monoxide poisoning by clinical characteristic, CT and MRI features Hunan Yi Ke Da Xue Xue Bao 2001 26 3 254 256 12536698 18 Guo J. Meng J. Han T. MRI-based comparison of brain damage between acute carbon monoxide poisoning and delayed encephalopathy after acute carbon monoxide poisoning Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2014 32 7 533 536 25182826 19 Xu X. M. Luo H. Rong B. B. Management of delayed encephalopathy after CO poisoning: an evidence-based narrative review Medicine 2019 98 49, article e18199 10.1097/MD.0000000000018199 31804341 20 Wang H. Li Y. Wu Q. Xu C. Liu Q. Combination of butylphthalide with umbilical mesenchymal stem cells for the treatment of delayed encephalopathy after carbon monoxide poisoning Medicine 2016 95 49, article e5412 10.1097/MD.0000000000005412 2-s2.0-85007489137 27930518 21 Wang P. Zeng T. Chi Z. F. Recovery of cognitive dysfunction in a case of delayed encephalopathy of carbon monoxide poisoning after treatment with donepezil hydrochloride Neurology India 2009 57 4 481 482 10.4103/0028-3886.55596 2-s2.0-70349427237 19770553 22 Zhang J. Wu H. Zhao Y. Zu H. Therapeutic effects of hydrogen sulfide in treating delayed encephalopathy after acute carbon monoxide poisoning American Journal of Therapeutics 2016 23 6 e1709 e1714 10.1097/MJT.0000000000000290 2-s2.0-84995747068 26164025 23 Wang W. Z. Ma G. Y. Zhao L. J. The relationship between lactate clearance rate and delayed encephalopathy after acute carbon monoxide poisoning Zhonghua Wei Zhong Bing Ji Jiu Yi Xue = Chinese critical care medicine 2013 25 10 622 626 10.3760/cma.j.issn.2095-4352.2013.10.011 2-s2.0-84892156433 24119701 24 Li Q. Song J. J. Zhang H. Y. Fu K. Lan H. B. Deng Y. Dexamethasone therapy for preventing delayed encephalopathy after carbon monoxide poisoning Biotechnic & Histochemistry : official publication of the Biological Stain Commission 2015 90 8 561 567 10.3109/10520295.2015.1019565 2-s2.0-84948088386 26052629 25 Doi H. Nagasaki H. Yamakawa K. Kubo Y. Ida M. Serial assessment of delayed encephalopathy after carbon monoxide poisoning using magnetic resonance imaging Chudoku kenkyu : Chudoku Kenkyukai jun kikanshi = The Japanese Journal of Toxicology 2012 25 299 301 23379216 26 Mao M. Rao P. Mou X. Guo L. Zhang L. Clinical observation on delayed encephalopathy after carbon monoxide poisoning treated with acupuncture to restore consciousness combined with hyperbaric oxygen treatment Zhongguo zhen jiu = Chinese Acupuncture & Moxibustion 2015 35 213 216 26062182 27 Terajima K. Igarashi H. Hirose M. Matsuzawa H. Nishizawa M. Nakada T. Serial assessments of delayed encephalopathy after carbon monoxide poisoning using magnetic resonance spectroscopy and diffusion tensor imaging on 3.0T system European Neurology 2007 59 1-2 55 61 10.1159/000109262 2-s2.0-37249067469 17917459