==== Front Innov Aging Innov Aging innovateage Innovation in Aging 2399-5300 Oxford University Press US 10.1093/geroni/igaa057.2740 igaa057.2740 Abstracts Session 7070 (Symposium) AcademicSubjects/SOC02600 Effects of Daily Vitamin D Supplementation on Objectively Measured Physical Activity: Results From the STURDY Trial Schrack Jennifer 1 Urbanek Jacek 2 Wanigatunga Amal 1 Juraschek Stephen 3 Mitchell Christine 1 Michos Erin 4 Roth David 2 Appel Lawrence 5 1 Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States 2 Johns Hopkins University, Baltimore, Maryland, United States 3 Harvard Medical School, Boston, Massachusetts, United States 4 Johns Hopkins School of Medicine, Baltimore, Maryland, United States 5 Johns Hopkins University School of Medicine, Baltimore, Maryland, United States 2020 16 12 2020 16 12 2020 4 Suppl 1 Program Abstracts from The GSA 2020 Annual Scientific Meeting “Turning 75: Why Age Matters”760 760 © The Author(s) 2020. Published by Oxford University Press on behalf of The Gerontological Society of America.2020This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract Cross-sectional evidence suggests older adults with higher serum vitamin D are more physically active, but whether long-term vitamin D supplementation attenuates age-related declines in physical activity (PA) is undefined. We examined the association between vitamin D supplementation and daily PA in 639 STURDY participants (aged 77 (5.4) years; 44% women) over up to 24-months. Participants were randomized to receive 200 (n=275), 1000 (n=168), 2000 (n=59), or 4000 (n=63) IU/day of vitamin D3. PA was measured using the Actigraph Link wrist-worn accelerometer 24 hours/day for 7-days at baseline, 3, 12, and 24 months. In linear mixed models adjusted for baseline PA level, total daily PA appeared to decline (β=-43.3 counts, p=0.06) annually for all groups and there was no difference by vitamin D3 dose (p for group*time =0.14). These results suggest daily vitamin D supplementation has no effect on quantities of daily PA.