==== Front
Innov Aging
Innov Aging
innovateage
Innovation in Aging
2399-5300 Oxford University Press US 

10.1093/geroni/igaa057.473
igaa057.473
Abstracts
Session 2880 (Poster)
Biology of Aging
AcademicSubjects/SOC02600
The Association of Freezer Storage Time With Vitamin K and Vitamin D Concentrations in Human Brain Tissue
Shea Kyla 1 Fu Xueyan 1 Dolinkowski Gregory 1 Dawson-Hughes Bess 1 Holland Thomas 2 Dhana Klodian 2 Booth Sarah 1 1 
Tufts University, Boston, Massachusetts, United States
2 
Rush University, Chicago, Illinois, United States
2020 
16 12 2020 
16 12 2020 
4 Suppl 1 Program Abstracts from The GSA 2020 Annual Scientific Meeting “Turning 75: Why Age Matters”144 145
© The Author(s) 2020. Published by Oxford University Press on behalf of The Gerontological Society of America.2020This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.Abstract
Vitamins K and D are present in the human brain and have been implicated in Alzheimer’s disease and related dementias (ADRD). Because the use of banked brain tissue in ADRD research is increasing, we evaluated the stability of vitamin K and vitamin D in human brain tissue over long-term freezer storage using samples obtained from the Rush Memory and Aging Project (n=500, mean age=91, 29% male). Specimens were stored at -80□C until analyzed. Vitamin K (menaquinone-4, MK4) and vitamin D (25(OH)D) were measured in four regions (mid-temporal and mid-frontal cortexes, cerebellum, anterior watershed white matter) and averaged across regions. Storage time was categorized into two-year increments. Differences in MK4 and 25(OH)D concentrations according to storage time were evaluated using general linear models. MK4 concentrations did not differ in brains stored ≤8 years (geometric mean±SEM MK4 pmol/g: storage ≤2.0 years=1.2±0.1, 2.1-4.0 years=1.2±0.1, 4.1-6.0 years=1.4±0.1, 6.1-8.0 years=1.4±0.2; p≥0.21). MK4 in brains stored >8.0 years (0.8±0.1 pmol/g) was 33% lower than the concentration in brains stored ≤2.0 years (p=0.005). The 25(OH)D concentrations did not differ in brains stored ≤6 years (geometric mean±SEM 25(OH)D pmol/g: storage ≤2.0 years=1.2±0.1, 2.1-4.0 years=1.1±0.1, 4.1-6.0 years=1.2±0.1; p≥0.37). The 25(OH)D concentration in brains stored >6.0 years was 31-37% lower than that in brains stored ≤2.0 years (6.1-8.0 years=0.8±0.06, >8.0 years=0.7±0.04; p<0.001). MK4 and 25(OH)D appeared to be stable in human brain tissue stored at -80oC for up to 8 and 6 years, respectively. Storage time merits consideration when designing and interpreting studies that relate brain nutrient concentrations to ADRD.