==== Front Eur J Gen Pract Eur J Gen Pract The European Journal of General Practice 1381-4788 1751-1402 Taylor & Francis 33292015 10.1080/13814788.2020.1846712 1846712 Version of Record Research Article Original Article Vitamin D status in the adult population of Bursa-Turkey O. Göktaş et al. https://orcid.org/0000-0003-3291-5187 Göktaş Olgun a https://orcid.org/0000-0003-4510-6282 Ersoy Canan b https://orcid.org/0000-0002-2382-290X Ercan Ilker c https://orcid.org/0000-0002-1953-7735 Can Fatma Ezgi d a Family Practice Unit, Uludağ University Family Health Center, Bursa, Turkey b Department of Internal Medicine, Division of Endocrinology and Metabolism, Uludağ University Faculty of Medicine, Bursa, Turkey c Department of Biostatistics, Uludağ University Faculty of Medicine, Bursa, Turkey d Department of Biostatistics, İzmir Katip Çelebi University Faculty of Medicine, İzmir, Turkey CONTACT Olgun Göktaş olgun_goktas@hotmail.comFamily Practice Unit, Uludağ University Family Health Center, Bursa, Turkey@OlgunGoktas 9 12 2020 2020 26 1 156162 © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2020 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background Vitamin D, along with parathyroid hormone (PTH) and calcitonin, is an important hormone that affects bone and calcium metabolism. Objectives To evaluate the vitamin D status and its seasonal variation in the adult population of Bursa. Methods Since there is not enough information about vitamin D levels, 25 OH vitamin D levels were analysed retrospectively from the records of 11,734 adult subjects (9142 women, 2592 men) admitted to 24 family health centres located in different districts of Bursa between 1 December 2017 and 30 November 2018. Some tests that can affect vitamin D levels, demographic features, and accompanying comorbidities were also evaluated. A face-to-face questionnaire was administered to subjects who were willing to answer (n = 2965). Results The mean serum vitamin D level was 16.6 ± 11.5 ng/mL in the entire population, 15.8 ± 11.7 ng/mL in women and 19.5 ± 9.9 ng/mL in men. The percentage of subjects with a vitamin D level <20 ng/mL was highest in March–May and lowest in the September–November period (80.7% and 69.9% for women, 77.5% and 32.9% for men, respectively). Conclusion Vitamin D levels <20 ng/mL are more prominent in women, and decline in spring, in the adult population of Bursa. These results are meaningful for the population living in Bursa, Turkey and the northern European region. Keywords General practice/family medicine vitamin D seasonal variation gender ==== Body KEY MESSAGES Vitamin D levels were <20 ng/mL in the entire population. Vitamin D levels <20 ng/mL are more prominent in women and decline in spring. The percentage of vitamin D levels <20 ng/mL was highest in summer and lowest in the autumn. Introduction Vitamin D, along with parathyroid hormone (PTH) and calcitonin, is an important hormone that affects bone and calcium metabolism. It also has extra-skeletal effects on insulin resistance and related disorders, immunity, and cancer. Its deficiency, insufficiency or excess in the body causes problems. Today, with the contribution of many factors, vitamin D deficiency or insufficiency is quite common and has become a significant health problem across the world. Although vitamin D deficiency is common, only those at a high risk of 25 (OH) D deficiency are evaluated, and community screening is not recommended [1–4]. Vitamin D is a steroid hormone, 80–90% produced in the skin from 7 dihydrocholesterol by incident ultraviolet (UV) B. The remaining 10–20% is derived from fatty fish, vegetable sources and foods rich in vitamin D. Vitamin D is a prohormone, activated in the liver and kidney. The angle of the incidence of short-wavelength UVB rays (290–315 nm) on the earth’s surface (Zenith angle), when not obtuse, is effective for the synthesis of vitamin D. Although this leads to less benefiting time in the northern European countries, vitamin D synthesis takes place between May and November in our (Turkey’s) latitude. The beneficial angle of the incidence of UVB is usually between 10:00 am to 03:00 pm. In the summer months, when the whole body is exposed to sunlight, at a minimal erythema dose, it synthesises vitamin D, equivalent to approximately 20,000 international units (IU) of oral dose. The minimal erythema dose is reached in approximately 15 min in people with light skin colour. However, the time to reach the minimal erythema dose is 3–4 times longer in individuals with darker skin colour [1,2,5–7]. Apart from the dark skin colour, people with a high risk of vitamin D deficiency are those who are elderly, obese, on medications that accelerate vitamin D metabolism, suffer from spontaneous fractures without trauma, osteoporosis, osteomalacia, malabsorption syndromes such as coeliac disease, inflammatory bowel disease, chronic kidney failure, chronic liver disease and hyperparathyroidism who have insufficient exposure to the sun [1–5,7]. Vitamin D status is determined according to 25 hydroxy (OH) vitamin D levels measured in serum. Vitamin D level may be sufficient (>30 ng/mL), insufficient (20–30 ng/mL), deficient (<20 ng/L) or seriously deficient (<10 ng/mL) [2,4,7]. Although a severe vitamin D deficiency, namely osteomalacia, is rarely seen, vitamin D deficiencies are quite common. The frequency of such deficiencies reported in adults who are 20 years of age and older is approximately 41.6% [8–10]. In different studies conducted in Turkey, the frequency of vitamin D deficiency in adults has been reported to be between 33% and 75% [4,11,12]. These deficiency rates are more critical for Turkish societies living in northern Europe for a long time. In this study, we aimed to retrospectively evaluate the vitamin D status and its seasonal variation in the adult population of Bursa province, 63.4% of whom live in urban areas and 34.7% in rural areas, with a latitude of approximately 40° located in the Southern Marmara region of western Turkey in the Northern hemisphere. Methods Study design After obtaining the necessary approvals, data on 25 OH vitamin D levels measured in patients admitted between 1 December 2017 and 30 November 2018 were analysed retrospectively from the records of adult patients aged ≥18 years who were admitted to 24 family medicine centres located in different districts of Bursa. A questionnaire when they apply to a family doctor for any reason was directed to subjects who were willing to answer (n = 2965, 25.3% of those who participated in the study, 61.2% of whom were women and 38.8% were men) concerning lifestyle and dietary habits related to vitamin D. The results were obtained from a single centralised laboratory. The method used for the measurement of 25 OH vitamin D was a new technology ECLIA (Electro-ChemiLuminescence Immunoassay) with the Roche Cobas E170 biochemistry analyser system. Besides serum 25 OH vitamin D levels, liver and renal function tests, glycaemic and lipid parameters, C-reactive protein (CRP) levels, demographic features, and accompanying comorbidities were obtained retrospectively from the patients’ records. Ethics The study was performed after the approval of the Health Directorate of Bursa of the Republic of Turkey Ministry of Health (Ref. 60429939/604.02) and the Clinical Ethics Committee of Bursa Uludağ University (Ref. 2017-19/14) and following the Declaration of Helsinki. Statistical analysis The consistency of continuous variables to normal distribution was tested using the Shapiro–Wilk test. Descriptive statistics are given as median (min–max) for continuous variables and n (%) for categorical variables. Kruskal–Wallis, Mann–Whitney U, Pearson chi-square and Fisher exact tests were used for comparisons between groups. Bonferroni correction was applied for binary group comparisons. Factors affecting low vitamin D levels were investigated using stepwise logistic regression analysis. Statistical analyses were performed using the IBM SPSS v.21 program. p < 0.05 was considered statistically significant. Results Vitamin D levels of 11,893 adult patients [9268 (77.9%) women, and 2625 (22%) men] were obtained. The data of 11,734 patients [9142 (77.9%) women, and 2592 (22%) men] whose vitamin D levels were studied at their first visit to a family doctor for any reason, and who did not receive any vitamin D treatment at the time of their visits were included in the study. A total of 2965 people participated in the questionnaire (n = 2965, 25.3% of those who participated in the study, 61.2% of whom were women, and 38.8% were men). Population characteristics and seasonal variation The average age of the 11,734 patients was 46.5 ± 16.9 years; the average age of women was 45.3 ± 16.4 years, and the average age of men was 51.1 ± 17.9 years. The mean serum vitamin D level in the entire population was calculated as 16.6 ± 11.5 ng/mL, which was 15.8 ± 11.7 ng/mL in women and 19.5 ± 9.9 ng/mL in men. Since seasonal variations affect UVB exposure, vitamin D levels were evaluated in both sexes according to seasons. To determine the season-dependent vitamin D levels, months were grouped as December–February (winter), March–May (spring), June–August (summer) and September–November (autumn). The number of admissions in winter, spring, summer and autumn were 3768 [2995 (79.4%) women, 773 (20.5%) men], 3279 [2533 (77.2%) women, 746 (22.7%) men], 2464 [1911 (77.5%) women, 553 (22.4%) men] and 2223 [1703 (76.6%) women, 520 (23.4%) men], respectively. A statistically significant difference was found in overall vitamin D levels amongst women and men when compared according to season (Table 1). To determine the seasonal variations in vitamin D status, patients in both sexes were divided into two groups with a cut-off level of 20 ng/mL (Table 1). Table 1. Vitamin D values by gender and seasons.   Vitamin D (ng/mL) (Overall)   Vitamin D categories     n Median (min–max) p value <20 ≥20 p value Women              December–February (I) 2995 11 (3–70)   2294 (76.6%) 701 (23.4%) <0.001  March–May (II) 2533 10.2 (3–140)   2044 (80.7%) 476 (19.3%)  June–August (III) 1911 10.3 (3–140) <0.001 1541 (80.6%) 357 (19.4%)  September–November (IV) 1703 14.8 (3–140)   1191 (69.9%) 491 (30.1%) All women              All seasons 9142 12.7 (3–140)   2344 (25.6%) 6798 (74.4%)   Men              December–February (I) 773 19 (3–70)   429 (55.5%) 344 (44.5%) <0.001  March–May (II) 746 14.8 (3–86.9)   578 (77.5%) 163 (22.5%)  June–August(III) 553 15.4 (3–88) <0.001 393 (71.1%) 156 (28.9%)  September–November (IV) 520 23.1 (3–71.8)   171 (32.9%) 339 (67.1%) All men              All seasons 2592 18.2 (3–88)   1110 (42.8%) 1482 (57.2%)   Total              All seasons 11,734           In terms of gender differences, a statistically significant difference was found between the vitamin D levels of men and women in different seasons (p < 0.001, p < 0.001). In women, no statistical differences were found in vitamin D levels < and ≥20 ng/mL between spring and summer periods. In men, however, statistically significant vitamin D levels < and ≥20 ng/mL were found in all periods. Vitamin D status, comorbidity and laboratory parameters When comorbid situations that might be associated with vitamin D levels were investigated, a statistically significant difference in vitamin D levels was found in patients suffering from type 2 diabetes, hypertension, dyslipidemia and atherosclerotic heart disease. Vitamin D deficiency (<20 ng/mL) appeared to occur less in the above-mentioned comorbid conditions (Table 2). Table 2. Association of comorbidities on vitamin D levels. Accompanying comorbidity Vitamin D status     n <20 (ng/mL) n ≥20 (ng/mL) p value Liver disease 8280 48 (0.6%) 3454 18 (0.5%) 0.699 Renal disease 8280 133 (1.6%) 3454 65 (1.9%) 0.291 Malabsorbtion 8280 101 (1.2%) 3454 34 (1%) 0.276 Inflammatory Bowel disease 8280 126 (1.5%) 3454 60 (1.7%) 0.395 Obesity 8280 123 (1.5%) 3454 38 (1.1%) 0.102 Type 2 diabetes 8280 197 (2.4%) 3454 113 (3.3%) 0.006 Hypertension 8280 2352 (28.4%) 3454 1250 (36.2%) <0.001 Dyslipidemia 8280 1082 (13.1%) 3454 601 (17.4%) <0.001 Atherosclerotic cardiovascular disease 8280 714 (8.6%) 3454 417 (12.1%) <0.001 Smokers 1014 8 (0.8%) 368 1 (0.3%) 0.459 Fracture            None (I)   1102 (85.8%)   459 (82.9%) 0.130  Bone Fragility (II)   152 (11.8%)   74 (13.4%)  Traumatic (III)   30 (2.3%)   21 (3.8%) In subjects with lower vitamin D levels, serum creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol, low-density lipoprotein (LDL) cholesterol and insulin values were lower, and phosphorus, calcium and CRP values were higher (Table 3). Table 3. Association of laboratory parameters on vitamin D levels. Laboratory parameter Vitamin D status     n <20 (ng/mL) n ≥20 (ng/mL) p value Calcium, Median (min–max) 2861 9.5 (5.2–12.3) 6653 9.4 (6.1–12) <0.001 Creatinine (mg/dL), Median (min–max) 7703 0.6 (0.1–5.7) 3250 0.7 (0.2–5) <0.001 ALT (U/L), Median (min–max) 7749 15 (1–411) 3261 16 (1–280) <0.001 AST (U/L), Median (min–max) 7565 16 (1–393) 3205 17 (7–144) <0.001 Glucose (mg/dL), Median (min–max) 7931 92 (36–482.5) 3333 92 (43–357) 0.090 HDL cholesterol (mg/dL), Median (min–max) 6840 52 (13–124) 2992 52 (17–145) 0.257 Total cholesterol (mg/dL), Median (min–max) 7372 182 (70–476) 3165 188.5 (74.5–423) <0.001 LDL cholesterol (mg/dL), Median (min–max) 6723 104 (11–372) 2954 109 (16–312) <0.001 Triglyceride (mg/dL) 7056 114 (22–1613) 3065 113 (28–810) 0.638 Insulin (mIU/L) 5342 8.7 (0.1–181.5) 2381 7.5 (0.2–146.6) <0.001 CRP (mg/L), Median (min–max) 6409 0.2 (0–31.5) 2737 0.1 (0–24.1) <0.001 Phosphorus, Median (min–max) 2425 3.5 (1.8–7.4) 5567 3.5 (1.4–7.3) 0.040 ALT: alanine aminotransferase; AST: aspartate aminotransferase; HDL: high-density lipoprotein; LDL: low-density lipoprotein; CRP: C-reactive protein. When gender, age, concomitant diseases, annual cigarette pack smoked, daily milk and dairy intake, clothing style and indoor work, sun exposure duration, sunscreen usage, and body mass index variables were considered in a stepwise logistic regression analysis, the levels of vitamin D were found to be affected by only gender and milk and dairy product consumption. The milk and dairy product consumption variables did not show a significant effect. It was observed that vitamin D level in women was 8.4 times lower than in men (Table 4). Table 4. Determination of factors affecting vitamin D level.   OR %95 Confidence interval p value Gender (Ref: Male) 8.4 3.8–18.4 <0.001 Milk (Ref: milk and dairy product consumption)     0.085   Dairy product 2.5 0.5–10.8 0.220   Milk 0.1 0–1.1 0.071   None 0.7 0.2–1.7 0.433 Model significance: p < 0.001. Discussion Main findings Low vitamin D levels are seen in increasing frequency in Turkey, although it has a sunny climate [10]. Our results indicated that low vitamin D levels are more prominent in women and in spring in the urban population of our city, Bursa. There are a few studies reported from Turkey, mainly conducted on female subjects, and older adults, and from different regions of Turkey [4,11–13]. This is the first population-based study conducted in Bursa with a higher number of subjects (n = 11,734; 9142 women and 2592 men) that has the power to represent the population living in Bursa, which was 3,056,120 in the year 2019 [14]. The average age of the 11,734 patients was 46.5 years, within the middle age group being active in life. The mean serum vitamin D level of the entire population was 16.6 ng/mL, which was <20 ng/mL (deficient range) [2,4,7,15,16]. The mean serum vitamin D level in women was lower than that in men (15.8 ng/mL vs. 19.5 ng/mL, respectively). When the serum vitamin D level was evaluated in terms of adequacy, it was found that 70.6% of the general population, 74.4% of women and 57.2% of men had a vitamin D level <20 ng/mL. These values were similar to those reported in other studies [4,17–23]. Seasonal variations are essential for sun exposure and vitamin D levels. In our study, a statistically significant difference was found in women and men when vitamin D values were compared according to season. In both women and men, vitamin D levels were lowest in the March–May period and highest in the September–November period (10.2 ng/mL and 14.8 ng/mL for women, 14.8 ng/mL and 23.1 ng/mL for men, respectively). Similarly, vitamin D levels <20 ng/mL were highest in the March–May period and lowest in the September–November period in both genders (80.7% and 69.9% for women, 77.5% and 32.9% for men, respectively). These results were similar to those of studies conducted in other European countries [17,23–26]. In our study, women had lower levels of vitamin D compared to men in all seasons, consistent with many studies in the literature [4,11,17,27]. In our study group, logistic regression analysis indicated that gender is an essential factor in vitamin D levels, and low vitamin D levels in women were 8.4 times higher than in men. This variation might be due to body fat content and distribution differences among men and women [28] as well as less exposure to sunlight due to clothing habits and less time spent outdoors for women in our study population. Comorbid conditions like nutrition, smoking and daily life habits may influence vitamin D levels. As a prohormone, vitamin D is activated in the liver and kidneys. Calcium is absorbed through the intestinal system via activated vitamin D. Low calcium intake, malabsorption and calcium metabolism disorders may lead to bone fractures. According to some studies, lower vitamin D levels are thought to be related to increased inflammation, hyperinsulinemia, insulin resistance and related disorders such as obesity, hypertension, dyslipidemia and atherosclerotic cardiovascular diseases. However, there are conflicting results [1,2,4–6,29,30]. When the accompanying comorbid situations were investigated in our study, contrary to some of the results in the literature, low vitamin D levels appeared to occur less in the presence of type 2 diabetes, hypertension, dyslipidaemia and atherosclerotic heart disease. Milk and dairy product consumption did not show a significant effect on vitamin D levels. Comparison with existing literature Our study results indicated that low vitamin D levels are more prominent in women and occur in spring, which is per the literature from Turkey and countries with similar to Turkey in geographical location [4,12,13,17,25]. Concerning the accompanying comorbid situations, contrary to some of the results in the literature, low vitamin D levels appeared to occur less in the presence of hypertension, dyslipidemia and atherosclerotic heart disease in our study [5,6,29,30]. Limitations Our study has some limitations. It was a retrospective data analysis. In the whole study population, vitamin D levels might be measured in some patients who did not have a risk of vitamin D deficiency; the questionnaire could be applied to a limited number of patients, the cut-off level of vitamin D taken as 20 ng/mL, according to some guidelines and literature, may lead to overdiagnosis of deficiency. Implications for clinical practice Due to the effects of vitamin D on general and skeletal health, it is crucial to increase awareness among physicians and patients about the vitamin D status of society. Our results are important to inform people to organise their lifestyle habits, leading to more conscious sun exposure and consuming healthy food rich in or enriched with vitamin D. Conclusion Our study indicated that low vitamin D levels in the population of Bursa are more prominent in women, occur in spring, and in situations such as living indoors. These results are meaningful for the population living in both Bursa and Turkey, and they are remarkable for both the Turkish and European populations in the northern European region. Acknowledgements We want to thank Editage (www.editage.com) for English language editing and ARGEV (The Turkish Family Medicine Research Development and Education Foundation) providing education before the study for their cooperation and our colleagues and family physicians who have contributed to this study by participating in all the districts of Bursa province. Mehtap Yılmaz: Çirişhane Family Health Center, Osmangazi, Bursa, Turkey; Bilgen Küçük: Küplüpınar Family Health Center, Osmangazi, Bursa, Turkey; H. Funda Arpacı: Küplüpınar Family Health Center, Osmangazi, Bursa, Turkey; Zeynep Özsevimli: Tuna Family Health Center, Osmangazi, Bursa, Turkey; Enes Mesutoğlu: Ali Bakgör Family Health Center, Osmangazi, Bursa, Turkey; Taner Ödemiş: Atıcılar Family Health Center, Osmangazi, Bursa, Turkey; Filiz Ödemiş: Atıcılar Family Health Center, Osmangazi, Bursa, Turkey; Alp Oktay: Demirtaş Family Health Center, Osmangazi, Bursa, Turkey; Şenay Karataş: Aykut Köroğlu Family Health Center, Osmangazi, Bursa, Turkey; Murat Girginer: Ertuğrulgazi Family Health Center, Yıldırım, Bursa, Turkey; Levent Gergil: Ortabağlar Family Health Center, Yıldırım, Bursa, Turkey; N. Nilüfer Yiğitalp: Ortabağlar Family Health Center, Yıldırım, Bursa, Turkey. Elif Kutlu: Teleferik Family Health Center, Yıldırım, Bursa, Turkey; Erhan Kutsal: Esenevler Family Health Center, Yıldırım, Bursa, Turkey; Tülay Gündüzcü: Beşevler Family Health Center, Nilüfer, Bursa, Turkey; H. Burcu Bulunmaz: Beşevler Family Health Center, Nilüfer, Bursa, Turkey; Mehmet Yaşar Kılıç: Fethiye Bulvar Family Health Center, Nilüfer, Bursa, Turkey; Olgun Göktaş: Uludağ University Family Health Center, Nilüfer, Bursa, Turkey. Emre Yalçıntaş: Büyükorhan Family Health Center, Büyükorhan, Bursa, Turkey; Yusuf Karayürek: Eşref Dinçer Family Health Center, Gemlik, Bursa, Turkey; Fahri Özaydın: Eşref Dinçer Family Health Center, Gemlik, Bursa, Turkey; Cemalettin Çinar: Şükrü Özdilek Family Health Center, İnegöl, Bursa, Turkey; Ertuğrul Demiray: 1 Nolu Entegre Family Health Center, Keles, Bursa, Turkey; Atıl Başar Bozkurt: Zeytinbağı Family Health Center, Mudanya, Bursa, Turkey; Muammer Yılmaz: Cevizli Family Health Center, Mustafakemalpaşa, Bursa, Turkey; Zafer Şengirgin: Çeltikçi Family Health Center, Mustafakemalpaşa, Bursa, Turkey; Nevin Özdilek: 4 Nolu Orkent Family Health Center, Orhangazi, Bursa, Turkey; Aykut Ardıç: 1 Nolu Siteler Family Health Center, Yenişehir, Bursa, Turkey; Nuh Esad Uyanık: Atatürk Family Health Center, Yenişehir, Bursa, Turkey. 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