==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 33366393 10.1080/23802359.2019.1693304 1693304 Version of Record Research Article Mitogenome Announcement The complete chloroplast genome sequence of Tainia cordifolia (Orchidaceae) Q.-d. Zheng et al. Zheng Qing-Dong Zhou Jie Ma Shan-Hu Chen Ming-Kun Xie Tai-Xiang Chen Juan Ai Ye Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, China CONTACT Ye Ai aiyefafu@163.comKey Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization, College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou350002, China 9 12 2019 2020 5 1 12 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2019 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Tainia cordifolia is a subtropical plant with significant ornamental value. Herein, we determined the complete chloroplast (cp) genome sequence of T. cordifolia using Illumina sequencing data. The whole cp genome is 158,089 bp in size, consisting of a pair of inverted repeats (IR 25,260 bp), a large single-copy region (LSC 86,876 bp), and a small single-copy region (SSC 20,693 bp). Plastid genome contains 136 genes, 88 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. What is more, a maximum-likelihood phylogenetic analysis demonstrated that T. cordifolia was most closely related to Oberonia japonica and Dendrobium salaccense. The cp genome will provide reference for the further investigation and research of T. cordifolia. Keywords Tainia cordifolia chloroplast genome Orchidaceae phylogenetic analysis National Natural Science Foundation of China10.13039/50110000180931700618 the Natural Science Foundation of Fujian Provincial10.13039/5011000033922016J01703 the Innovation Project of Fujian Agriculture and Forestry UniversityKFA17331A This work was supported by the National Natural Science Foundation of China [No. 31700618], the Natural Science Foundation of Fujian Provincial [No. 2016J01703], and the Innovation Project of Fujian Agriculture and Forestry University [No. KFA17331A]. ==== Body Orchidaceae is one of the largest family of monocotyledons (Cameron et al. 1999). It not only has a long history of cultivation and numerous varieties, but also has ecological value. Tainia cordifolia is a terrestrial herb belongs to the Orchidaceae family, growing in the evergreen broad-leaved forest at 580–1900 meters above sea level or in the mountain stream (Chen and Wood 2009). Therefore, we reported the complete chloroplast genome (cp) of T. cordifolia based on Illumina pair-end sequencing data, which would be helpful for its evolution and genetics research. The leaf sample was collected from Gushan Mountains (26°05′28″N, 119°38′63″E), Fuzhou city, Fujian province, China. The specimen was stored at Fujian Agriculture and Forestry University (specimen code FAFU08457). The total genomic DNA was extracted from fresh leaves according to the methods described by Doyle and Doyle (1987) and sequencing was carried out by the Illumina pair-end technology. Raw reads were filtered using NGS QC Toolkit (Patel and Jain 2012). Clean reads were first aligned to Calanthe triplicata (GenBank Accession No. NC_024544) and Calanthe davidii (GenBank Accession No. NC_037438). Filtered reads were then assembled into contigs in the software Platanus version 1.2.4 (Kajitani et al. 2014). The physical map of the new chloroplast genome was generated using OGDRAW (Lohse et al. 2013). Finally, the validated complete cp genome sequence was submitted to GenBank with accession number MN577470. The complete cp genome of T. cordifolia is 158,089 bp in length, containing a large single-copy (LSC) region of 86,876 bp, a small single-copy (SSC) region of 20,693 bp, and two inverted repeat (IR) regions of 25,260 bp. The new sequence has a total of 136 genes, including 88 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. The overall GC-content of the whole plastome is 37.3%, whereas the corresponding values of the LSC, SSC, and IR regions are 54.95%, 13.09%, and 15.98%, respectively. To further investigate its phylogenetic position, 12 complete cp genomes of Orchidaceae (Cattleya crispata, Cattleya liliputana, Masdevallia picturata, Corallorhiza odontorhiza, Oncidium ‘Gower Ramsey’, Erycina pusilla, Cymbidium tortisepalum, Calanthe triplicata, Calanthe davidii, Tainia cordifolia, Oberonia japonica, Dendrobium salaccense) were aligned using HomBlocks pipeline (Bi et al. 2018). All the data were downloaded from NCBI GenBank. RAxML-HPC2 on XSEDE version 8.2.10 (Stamatakis 2014) was used to construct a maximum likelihood tree. The ML tree analysis indicated that Oberonia japonica and Dendrobium salaccense closely related to T. cordifolia with 100% bootstrap support (Figure 1). Figure 1. A phylogenetic tree was constructed based on 12 complete chloroplast genome sequences of Orchidaceae. All the sequences were downloaded from NCBI GenBank. Disclosure statement There are no conflicts of interest for all the authors including the implementation of research experiments and writing this article. ==== Refs References Bi G, Mao Y, Xing Q, Cao M. 2018. HomBlocks: a multiple-alignment construction pipeline for organelle phylogenomics based on locally collinear block searching. Genomics. 110 (1 ):18–22.28780378 Cameron KM, Chase MW, Whitten WM, Kores PJ, Jarrell DC, Albert VA, Yukawa T, Hills HG, Goldman DH. 1999. A phylogenetic analysis of the Orchidaceae: evidence from rbcL nucleotide sequences. American Journal of Botany, 86(2):208–224. Chen SC, Wood JJ. 2009. Orchidaceae. Flora of China. Vol. 25. Beijing Science Press. Doyle JJ, Doyle JL. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 19 :11–15. Lohse M, Drechsel O, Kahlau S, Bock R. 2013. 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