==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 33366449 10.1080/23802359.2019.1698340 1698340 Version of Record Research Article Mitogenome Announcement Characterization and phylogenetic analysis of the complete mitochondrial genome of Conidiobolus sp. (Entomophthorales: Ancylistaceae) X. Sun et al. Mitochondrial DNA Part B Sun Xiaorong a* Su Dan a* Gui Wenjuan b Luo Feng a Chen Yue a a Chengdu Bio-HT Company Limited, Chengdu, China; b General Hospital of Ningxia Medical University, Yinchuan, China * These authors contribute equally to this work. CONTACT Yue Chen yue.chen@bio-ht.cnChengdu Bio-HT Company Limited, Chengdu, China 12 12 2019 2020 5 1 121122 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2019 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract In the present study, we presented the complete mitochondrial genome of an entomophthoroid fungus Conidiobolus sp. The mitogenome of Conidiobolus sp. has a total length of 26,612 bp, with the base composition as follows: A (44.22%), T (27.10%), C (10.99%) and G (17.68%). The mitogenome contains 19 protein-coding genes, 2 ribosomal RNA genes (rRNA), and 23 transfer RNA (tRNA) genes. The taxonomic status of the Conidiobolus sp. mitogenome exhibited a close relationship with the mitogenome of Conidiobolus heterosporus. Keywords Conidiobolus entomophthoroid fungus mitochondrial genome phylogenetic analysis ==== Body Conidiobolus, a fungal genus in the family Ancylistaceae, is widely distributed in soil and among plants, insects, and amphibians (de Godoy et al. 2017). So far, 37 species have been described in this genus (Nie et al. 2018). C. coronatus causes conidiobolomycosis, a chronic granulomatous disease of submucosal and subcutaneous tissues (Moncada et al. 2016). Species of this genus have been widely used to study phylogenetic relationships of basal fungi (Nie et al. 2019). The complete mitogenome of Conidiobolus sp. will provide a reference for understanding the phylogeny and evolution of the basal fungi. The specimen (Conidiobolus sp.) was isolated from soil in Guangyuan, Sichuan, China (104.41 E; 31.40 N) and was stored in Sichuan Academy of Agricultural Sciences Center for Culture Collection (No. Cps1). The total genomic DNA of Conidiobolus sp. was extracted using Fungal DNA Kit D3390-00 (Omega Bio-Tek, Norcross, GA, USA) and purified through a Gel Extraction Kit (Omega Bio-Tek, Norcross, GA, USA). Purified genomic DNA was also stored in the Sichuan Academy of Agricultural Sciences Center for Culture Collection (No. DNA_Cps1). We constructed the sequencing libraries with purified genomic DNA following the instructions of NEBNext® Ultra™ II DNA Library Prep Kit (NEB, Beijing, China). Whole genomic sequencing (WGS) was performed by the Illumina HiSeq 2500 Platform (Illumina, SanDiego, CA, USA). The raw data obtained was first passed through a series of quality control steps (Li et al. 2018a). The complete mitogenome was de novo assembled using the clean data as implemented by SPAdes 3.9.0 (Bankevich et al. 2012). Gaps among contigs were filled by using MITObim V1.9 (Hahn et al. 2013). The obtained mitogenome was annotated using the MFannot tool (http://megasun.bch.umontreal.ca/cgi-bin/mfannot/mfannotInterface.pl), combined with manual corrections. tRNAs were annotated by tRNAscan-SE (Lowe and Eddy 1997). The total length of Conidiobolus sp. circular mitogenome is 26,612 bp. This mitogenome was submitted to GenBank database under accession No. MN640580. The circular mitogenome contains 19 protein-coding genes, 2 ribosomal RNA genes (rns and rnl), and 23 transfer RNA (tRNA) genes. The base composition of the genome is as follows: A (44.22%), T (27.10%), C (10.99%) and G (17.68%). To validate the phylogenetic position of Conidiobolus sp., we constructed phylogenetic trees of 12 closely related species. Bayesian analysis (BI) was used to construct the phylogenetic trees with the combined 14 core protein-coding genes according to Li (2018b, 2018c). As shown in the phylogenetic tree (Figure 1), the taxonomic status of the Conidiobolus sp. mitogenome exhibited a close relationship with the mitogenome of C. heterosporus (Nie et al. 2019). Figure 1. Molecular phylogenies of 12 species based on Bayesian inference analysis of the combined mitochondrial gene set (14 core protein-coding genes). Node support values are Bayesian posterior probabilities (BPP). Mitogenome accession numbers used in this phylogeny analysis: Tricholoma matsutake (JX985789), Laccaria amethystina (MK697669), Lactarius volemus (MH319474), Ganoderma sinense (KF673550), Coniferiporia sulphurascens (MK623260), Cantharellus appalachiensis (MG602716), Cantharellus cibarius (KC573037), Cantharellus lutescens (MG602719), Tremella fuciformis (MF422647), Zancudomyces culisetae (AY863213), Conidiobolus heterosporus (MK049352). Disclosure statement The authors have declared that no competing interests exist. ==== Refs References Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, et al. 2012. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 19 (5 ):455–477.22506599 de Godoy I, de Campos CG, Pescador CA, Galceran JV, Cândido SL, Dutra V, Nakazato L. 2017. Experimental infection in gerbils by Conidiobolus lamprauges. Microb Pathog. 105 :251–254.28258001 Hahn C, Bachmann L, Chevreux B. 2013. Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads: a baiting and iterative mapping approach. Nucleic Acids Res. 41 (13 ):e129.23661685 Li Q, Chen C, Xiong C, Jin X, Chen Z, Huang W. 2018a. Comparative mitogenomics reveals large-scale gene rearrangements in the mitochondrial genome of two Pleurotus species. 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