==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 33366453 10.1080/23802359.2019.1692724 1692724 Version of Record Research Article Mitogenome Announcement The complete chloroplast genome of Quercus robur ‘Fastigiata’ L. Feng et al. Feng Lijuan a Yang Xuemei a Jiao Qiqing a Wang Chuanzeng b Yin Yanlei a a Shandong Institute of Pomology, Taian, Shandong, China; b Shandong Academy of Agricultural Sciences, Jinan, Shandong, China CONTACT Yanlei Yin yylei66@sina.comShandong Institute of Pomology, Taian, Shandong, China 11 12 2019 2020 5 1 129130 © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. 2020 The Author(s) https://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract The Quercus robur ‘Fastigiata’ is an important ornamental plant, in which the complete chloroplast genome (accession no. MN562095) was identified and sequenced. The genome size is 161,172 bp, with a large single-copy (LSC, 90,505 bp) region, a small single-copy (SSC, 18,997 bp) region, and two inverted repeat regions (IRs, 25,835 bp each). A total of 134 genes are successfully annotated, including 89 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The phylogenetic relationships inferred that Q. robur ‘Fastigiata’ is closely related to Quercus mongolica, Quercus wutaishanica, and Quercus dentata. Keywords Quercus robur ‘Fastigiata’ chloroplast genome Illumina sequencing phylogenetic analysis Shandong Key Research and Development Projects2018GNC111009 Shandong Agricultural Improvement Seed Projects2017LZN023 Agricultural Science and Technology Innovation Engineering Discipline Team of Shandong Academy of Agricultural SciencesCXGC2018E22 This work was supported by the Shandong Key Research and Development Projects [2018GNC111009], Shandong Agricultural Improvement Seed Projects [2017LZN023], and Agricultural Science and Technology Innovation Engineering Discipline Team of Shandong Academy of Agricultural Sciences [CXGC2018E22]. ==== Body The Quercus robur ‘Fastigiata’ is a very suitable plant for botanical gardens and parks, this is mainly distributed in France, Italy, USA, and China. It has high ornamental value and is suitable for planting in botanical gardens and parks (Iglesias-Díaz et al. 2000). The complete chloroplast (cp) genome can provide valuable genomic information for the conservation and restoration of rarely relict species (Qiang et al. 2019). The cp genome resources have been explored in Quercus acutissima (GenBank accession no. MH607377) (Li et al. 2018), Quercus baronii (GenBank accession no. KT963087) (Yang et al. 2015), and Quercus dentata (GenBank accession no. MG967555) (Hu et al. 2019), but the Q. robur ‘Fastigiata’ has not been fully sequenced. In this study, we sequenced and analyzed the cp genome of Fastigiata based on Illumina pair-end sequencing and compared it with other genus cp genome sequences. It is helpful for future genetic studies on this and other related species. The voucher specimen (accession no. SDZXL00136) of Q. robur ‘Fastigiata’ tree was deposited in the Taidong field of Shandong Institute of Pomology, Shandong Province, China (36.20°N, 117.12°E). Total genomic DNA was extracted from young leaf tissue using the DNeasy Plant Mini Kit (Qiagen, Venlo, Netherlands). DNA was sequenced using the Illumina HiSeq 2500 platform by Genepioneer Biotechnologies (Nanjing, China). The raw paired-end reads were filtered using the fastp program (Chen et al. 2018). The high-quality reads were applied to a de novo assembly performed using GetOrganelle (Jin et al. 2018). Annotation was completed by the online program GeSeq (Tillich et al. 2017) and the result was manually adjusted where necessary using Geneious (Kearse et al. 2012). The complete cp genome was deposited in the GenBank (accession no. MN562095). The complete cp genome of Q. robur ‘Fastigiata’ was 161,172 bp in length having 36.83% of total GC content. It is made up of a large single-copy (LSC, 90,505 bp) region, a small single-copy (SSC, 18,997 bp) region, and two inverted repeat regions (IRs, 25,835 bp each). A total of 134 genes are successfully annotated, including 89 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The content of protein-coding genes, tRNA genes, and rRNA genes is 66.4, 27.6, and 6.0%, respectively. The tRNA genes are distributed throughout the whole genome with 21 in the LSC, three in the SSC, and 13 in the IR regions, while rRNAs are only situated in the IR regions. Seven genes of tRNA (trnA-UGC, trnI-CAU, trnI-GAU, trnL-CAA, trnN-GUU, trnR-ACG, and trnV-GAC) had two copies and all four rRNA species (rrn4.5, rrn5, rrn16, and rrn23) also had two copies. Among the protein-coding genes, three genes (clpP, rps12, and ycf3) contained two introns, and other nine genes (atpF, ndhA, ndhB, petB, petD, rpl16, rpl2, rpoC1, and rps16) had one intron each. The complete cp genome sequences of other Quercus, Trigonobalanus, Castanea, Fagus, Lithocarpus, and Castanopsis species were used to construct the phylogenetic tree, and Acer catalpifolium (GenBank accession no. MF179637) and Acer wilsonii (GenBank accession no. MG012225) as the outgroup. Using MAFFT v7.3 (Katoh and Standley 2013), we aligned 22 cp genomes of species. The maximum-likelihood (ML) phylogenetic tree was constructed by the IQ-TREE with the best-fit model identified using ModelFinder (Kalyaanamoorthy et al. 2017). The result showed that Q. robur ‘Fastigiata’ is closely related to Quercus mongolica (GenBank accession no. MK564083), Quercus wutaishanica (GenBank accession no. MK059753), and Q. dentata (GenBank accession no. MG967555) (Figure 1). This newly reported cp genome will provide valuable information for genetic evolution and molecular breeding studies of Quercus. Figure 1. The phylogenetic tree of 22 complete chloroplast genome sequences based on the best maximum-likelihood (ML). Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Chen S, Zhou Y, Chen Y, Gu J. 2018. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 34 (17 ):i884–i890.30423086 Hu HL, Zhang JY, Li YP, Xie L, Chen DB, Li Q, Liu YQ, Hui SR, Qin L. 2019. The complete chloroplast genome of the daimyo oak, Quercus dentata Thunb. Conserv Genet Resour. 11 (4 ):409–411. Iglesias-Díaz MI, Fernández-Lorenzo JL, Gutiérrez-Araujo O. 2000. Macro- and micropropagation of a centenarian clone of Quercus robur ‘fastigiata’. Acta Hortic. 536 :331–337. Jin JJ, Yu WB, Jun B, Yang JB, Song Y, Yi TS, Li DZ. 2018. GetOrganelle: a simple and fast pipeline for de novo assembly of a complete circular chloroplast genome using genome skimming data. bioRxiv. 4 :256479. Kalyaanamoorthy S, Minh BQ, Wong TKF, von Haeseler A, Jermiin LS. 2017. 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