==== Front Microbiol Resour Announc Microbiol Resour Announc ga mra MRA Microbiology Resource Announcements 2576-098X American Society for Microbiology 1752 N St., N.W., Washington, DC MRA00728-20 10.1128/MRA.00728-20 Genome Sequences Draft Genome Sequence of Serratia marcescens Strain ZZCCN01, Isolated from the Cardiac Blood of a Beef Cow Zhang Yu-Long a Huang Fang-Yuan a Yao Cai-Xia a Cai Dong-Jie a Wang Zhi-Sheng b https://orcid.org/0000-0002-5547-4436Zuo Zhi-Cai a a Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China b Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan, China Bruno Vincent EditorUniversity of Maryland School of Medicine Address correspondence to Zhi-Sheng Wang, wangzs67@163.com, or Zhi-Cai Zuo, zzcjl@126.com.Citation Zhang Y-L, Huang F-Y, Yao C-X, Cai D-J, Wang Z-S, Zuo Z-C. 2020. Draft genome sequence of Serratia marcescens strain ZZCCN01, isolated from the cardiac blood of a beef cow. Microbiol Resour Announc 9:e00728-20. https://doi.org/10.1128/MRA.00728-20. 3 12 2020 12 2020 9 49 e00728-204 8 2020 5 11 2020 Copyright © 2020 Zhang et al.2020Zhang et al.This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.Serratia marcescens strain ZZCCN01 was isolated from the cardiac blood of a dead beef cow with a lung infection and a foam-like secretion from the nostril. Here, we introduce the 5.1-Mb draft genome sequence, which comprises 105 scaffolds, and the corresponding annotation. ABSTRACT Serratia marcescens strain ZZCCN01 was isolated from the cardiac blood of a dead beef cow with a lung infection and a foam-like secretion from the nostril. Here, we introduce the 5.1-Mb draft genome sequence, which comprises 105 scaffolds, and the corresponding annotation. cover-dateDecember 2020 ==== Body ANNOUNCEMENT Serratia marcescens is a Gram-negative, clinically opportunistic pathogen. It can cause infection and sepsis targeting the lung and urinary tract when the immune system is impaired (1, 2), as well as systemic infections and death (3). Serratia marcescens strains were obtained from the blood samples from a dead beef cow through pathological dissection. Cardiac blood was collected from the beef cow aseptically with vacuum blood collection tubes, preserved in ice-cold condition, and transported to the laboratory. Samples were streaked onto lysogeny broth agar and incubated overnight at 37°C for 18 h. Single colonies were utilized to inoculate lysogeny broth, from which genomic DNA was isolated with the TIANamp bacterial DNA kit (Tiangen Biotech, Beijing, China). For sequencing, extracted DNA was utilized to prepare shotgun libraries for Illumina sequencing following the instructions of the manufacturer (Illumina, San Diego, CA, USA). A NEBNext Ultra DNA library prep kit was used to make the Illumina sequencing library (New England Biolabs, Beverly, MA, USA). Sequencing by an Illumina HiSeq instrument resulted in 9,683,553-bp paired-end Illumina reads (150 bp). The reads were trimmed using Trimmomatic version 3.5.0 with default settings. The initial hybrid de novo assembly was performed using SPAdes software version 3.9.0 with default settings (4, 5) and resulted in 105 scaffolds. The correction was performed using PrlnSeS-G version 1.0.0 with default settings (6). The genome of Serratia marcescens strain ZZCCN01 had an overall G+C content of 59%, and the N50 value of the contigs from the genome assembly was 150,116 bp. The genome sequence was annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) (https://www.ncbi.nlm.nih.gov/genome/annotation_prok/), and the results showed that it has 4,910 genes, 4,795 coding DNA sequences (CDSs), 80 tRNAs, 10 rRNAs, and 25 noncoding RNAs (ncRNAs). The genome sequence was annotated using the Virulence Factor Database (http://www.mgc.ac.cn/VFs), which yielded 1,339 virulence factors or virulence factor-related genes, such as SLS, which lyses a wide variety of eukaryotic cells, including myocardial cells, kidney cells, platelets, lymphocytes, and neutrophils (7); htpB, whose product, hsp60, was involved in apoptosis (8); mip, the macrophage infectivity potential gene (9); pglJ, which participates in the colonization of intestinal niches in a wide variety of hosts (10); and farA, which mediates the resistance to antimicrobial long-chain fatty acids. The drug resistance functions were annotated by the Comprehensive Antibiotic Resistance Database (https://card.mcmaster.ca/), which yielded 4,627 antibiotic resistance-associated genes, such as OXA-58, a class D β-lactamase (11); folP, a sulfonamide resistance gene (12); AAC6, an aminoglycoside resistance gene (13); and fsr, a fosmidomycin resistance gene (14). ZZCCN01 was found to contain the small multidrug resistance protein (AcrAB family) and multidrug efflux pumps (15), indicating its potential to become a multidrug-resistant strain. Serratia marcescens infection is a global public health problem. ZZCCN01 was isolated from the cardiac blood of a beef cow. Therefore, it is of great significance to determine the genomic sequence of ZZCCN01 for better understanding of the infection mechanisms of Serratia marcescens in humans and animals. Data availability. This whole-genome shotgun sequencing project has been deposited at DDBJ/EMBL/GenBank under the accession no. JABTVN000000000.1. The associated BioProject, SRA, and BioSample accession numbers are PRJNA626063, SRP257993, and SAMN14615110, respectively. ACKNOWLEDGMENTS This work, including the efforts of Z.-C.Z. and D.-J.C., was funded by the National Key Research and Development Project (2018YFD0501800), the Sichuan Science and Technology Program (2018NZ0002, 2019YFQ0012), and the Sichuan Beef Cattle Innovation Team of the National Modern Agricultural Industry Technology System (SCCXTD-2020-13). ==== Refs REFERENCES 1. Lakhani NA , Narsinghani U , Kumar R 2015 Necrotizing fasciitis of the abdominal wall caused by Serratia marcescens . Infect Dis Rep 7 :5774 . doi:10.4081/idr.2015.5774 .26294949 2. Kim SB , Jeon YD , Kim JH , Kim JK , Ann HW , Choi H , Kim MH , Song JE , Ahn JY , Jeong SJ , Ku NS , Han SH , Choi JY , Song YG , Kim JM 2015 Risk factors for mortality in patients with Serratia marcescens bacteremia . Yonsei Med J 56 :348 –354 . doi:10.3349/ymj.2015.56.2.348 .25683980 3. 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