==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1687041 1687041 Version of RecordResearch Article Mitogenome Announcement Characterization of the complete chloroplast genome of an annual halophyte, Chenopodium glaucum (Amaranthaceae) Y. Yao et al.Yao Yan Li Xiao-Tong Wu Xi-Yue https://orcid.org/0000-0001-7586-4741Fan Shou-Jin Zhang Xue-Jie https://orcid.org/0000-0003-1201-2554Qu Xiao-Jian Key Lab of Plant Stress Research, College of Life Sciences, Shandong Normal University, Ji’nan, Shandong, China CONTACT Xue-Jie Zhang zhangxuejie@sdnu.edu.cn; Xiao-Jian Qu quxiaojian@sdnu.edu.cnKey Lab of Plant Stress Research, College of Life Sciences, Shandong Normal University, Ji’nan, Shandong, China 8 11 2019 2019 4 2 3898 3899 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract The complete chloroplast genome (plastome) of Chenopodium glaucum, an annual halophytic herb, was determined. The plastome was 152,191 bp in size, containing a large single-copy region (83,675 bp), a small single-copy region (18,130 bp), and two inverted repeats regions (25,193 bp). The overall GC content of this plastome was 37.2%. In total, 113 unique genes were annotated including 79 protein-coding genes (PCGs), 30 tRNAs and 4 rRNAs. Phylogenomic analysis showed that C. glaucum was sister to C. album. Keywords Chenopodium glaucumplastomephylogenomics ==== Body Chenopodium glaucum is an annual halophytic herb from Amaranthaceae with worldwide distribution. Halophytes such as Suaeda salsa has succulent leaf to adapt to saline conditions, while C. glaucum has inconspicuous morphological specialization under salt stress (Li et al. 2008; Sui et al. 2010; Yang et al. 2010; Song et al. 2011; Li et al. 2012; Cheng et al. 2014; Guo et al. 2015; Song and Wang 2015; Sui 2015; Wang et al. 2015; Chen et al. 2016; Song et al. 2016; Zhou et al. 2016; Song et al. 2017; Sui et al. 2017; Guo et al. 2018; Liu et al. 2018). In addition, C. glaucum also has positive environmental impacts such as helping to improve the soil texture and reduce soil salinity, and its leaves can be used as feed (Hong et al. 2017). In this study, we reported the plastome of C. glaucum, which would provide fundamental genetic resources for studying this important species as well as resolving its phylogenetic position. Fresh leaves of C. glaucum were collected from Laishan District (Shandong, China; 121°23′N, 37°22′E). Voucher specimen (330138) was deposited at Kunming Institute of Botany, Chinese Academy of Sciences. The modified CTAB method was used to extract total genomic DNA (Wang et al. 2013). Considering the limited fresh sample, chloroplast DNA was not extracted directly (Liu et al. 2017). Total genomic DNA was used for library preparation and paired-end (PE) sequencing by the Illumina MiSeq at Novogene (Beijing, China). Plastome was assembled using Organelle Genome Assembler (OGA; Qu 2019). Annotation was performed with Plastid Genome Annotator (PGA; Qu et al. 2019), coupled with manual correction using Geneious v8.0.2. A maximum-likelihood (ML) tree was reconstructed to determine the phylogenetic placement of C. glaucum using RAxML v8.2.10 (Stamatakis 2014), including tree robustness assessment using 1000 rapid bootstrap replicates with the GTRGAMMA substitution model, based on alignment of 79 shared protein-coding genes using MAFFT v7.313 (Katoh and Standley 2013). The complete plastome of C. glaucum (GenBank accession number: MN422308) was 152,191 bp in length, composed of a large single-copy region (83,675 bp), a small single-copy region (18,130 bp), and a pair of inverted repeats (25,193 bp). The overall GC content was 37.2%. A total of 113 unique genes were annotated in this plastome, including 79 PCGs, 30 tRNAs, and 4 rRNAs. Thirteen PCGs and eight tRNAs contained introns among the annotated genes. The ML phylogenetic tree showed that C. glaucum was sister to C. album (Figure 1). Figure 1. A maximum-likelihood (ML) tree inferred from 79 plastome genes. Four Amaranthus species from Amaranthaceae are used as outgroup. The numbers on branches are bootstrap support values. Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Chen TS , Yuan F , Song J , Wang BS 2016 Nitric oxide participates in waterlogging tolerance through enhanced adventitious root formation in the euhalophyte Suaeda salsa . Functional Plant Biol . 43 (3 ):244 –253 . Cheng S , Yang Z , Wang MJ , Song J , Sui N , Fan H 2014 Salinity improves chilling resistance in Suaeda salsa . Acta Physiol Plant . 36 (7 ):1823 –1830 . Guo JR , Li YD , Han GL , Song J , Wang BS 2018 NaCl markedly improved the reproductive capacity of the euhalophyte Suaeda salsa . Functional Plant Biol . 45 (3 ):350 –361 . Guo JR , Suo SS , Wang BS 2015 Sodium chloride improves seed vigour of the euhalophyte Suaeda salsa . Seed Sci Res . 25 (3 ):335 –344 . 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