==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1687362 1687362 Version of RecordResearch Article Mitogenome Announcement Characterization of the complete mitochondrial genome of Odontobutis platycephala collected from Nakdong River, South Korea M. J. Alam et al.https://orcid.org/0000-0002-3594-8147Alam Md. Jobaidul a Kim Kyung Su b https://orcid.org/0000-0002-2566-1636Andriyono Sapto ac Park Wongyu d Baek Hae-Ja d https://orcid.org/0000-0002-4800-0566Je Jae-Young e https://orcid.org/0000-0003-1357-5893Kim Hyun-Woo ad a Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University, Busan, Republic of Korea; b Gyeongsangnam-do Freshwater Fish Research Center, Miryang, Republic of Korea; c Fisheries and Marine Faculty, C Campus Jl, Universitas Airlangga, Surabaya, Indonesia; d Department of Marine Biology, Pukyong National University, Busan, Republic of Korea; e Department of Marine-Bio Convergence Science, Pukyong National University, Busan, Republic of Korea CONTACT Hyun-Woo Kim kimhw@pknu.ac.krDepartment of Marine Biology, Pukyong National University, Busan48513, Republic of Korea 8 11 2019 2019 4 2 3908 3909 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract The complete mitochondrial genome of Odontobutis platycephala collected from a native Korean river was determined by the bioinformatics assembly of the next-generation sequencing (NGS) reads. The circular mitogenome was 17,590 bp length which harbored canonical 13 protein-coding genes, 22 tRNAs, and 2 rRNAs, which was identical to those of family Odontobutidae. Twenty-eight genes were located on H strand, whereas remaining nine genes were on L strand. Except for COX1 gene (GTG), other 12 protein-coding genes were predicted typical start codons (ATG). Among the currently known mitogenome sequences, O. platycephala showed highest identity (96.98%) to Korean haplotype of O. platycephala (NC010199). Keywords Next-generation sequencingOdontobutis platycephalamitochondrial genome ==== Body Fish in the genus Odontobutis are the freshwater sleepers native to East Asia and three species in the genus are currently reported in Korea; Odontobutis platycephala and Odontobutis interrupta, and Odontobutis obscura (Chae 1999; Iwata and Sakai 2002; Jun et al. 2016). We here report the complete mitochondrial genome of O. platycephala, which has been collected from a tributary of Nakdong River, South Korea (E128°06′27.73″, N35°32′20.96″) in 2018. The collected specimen and its DNA were stored at the Marine Biodiversity Institute of Korea (MABIK GR00002616). Its COI region showed 99.82% nucleotide sequence identity to the Korean haplotype of O. platycephala (JX679047). The complete mitochondrial DNA from the specimen was isolated by a commercially available kit (Abcam, Cambridge, MA, USA), then the complete mitochondrial genome was determined by the Illumina MiSeq sequencing method. The TruSeq® RNA library preparation kit V2 (Illumina, San Diego, CA, USA) was used with the fragmented mitochondrial DNA by Covaris M220 Focused-Ultrasonicator (Covaris Inc., Woburn, MA, USA). The complete circular mitochondrial DNA was constructed by the bioinformatics assembly of the raw reads using Geneious software version 11.0.2 (Biomatters Ltd., Auckland, New Zealand)(Kearse et al. 2012). The secondary structures of 22 tRNAs were predicted by ARWEN program (Laslett and Canbäck 2008). The complete circular mitogenome of O. platycephala (MN416814) was 17,590 bp in length, which consisted of 13 protein-coding genes, 22 tRNAs, and 2 ribosomal RNAs (12S and 16S). As shown in the other fish in the genus Odontobutis, the unusual additional non-coding region was identified between ND4 and ND5 as a result of a rearrangement of tRNAs between them (Ki et al. 2008). Total of 12 protein-coding genes was encoded on heavy strand (H), whereas ND6 was encoded on light strand (L). The typical control region (1287 bp) was identified between tRNA-Pro and tRNA-Phe, while the origin of light strand (OL, 32 bp) was located between tRNA-Asn and tRNA-Cys at the WANCY tRNA cluster. Twelve protein-coding genes were started with typical ATG, whereas COX1 started with GTG. The incomplete stop codons (TA–/T––) were predicted in eight genes including ND1, ND2, ATP6, COX2, COX3, ND3, ND4, and Cytb genes. A phylogenetic tree for the currently reported mitogenomes of Gobiiformes was constructed using the MEGA version 7 program with minimum evolution algorithm (Kumar et al. 2016). Among six mitogenomes in the same genus, O. platycephala collected from the Nakdong River showed the highest nucleotide sequence identity (96.98%) to the Korean haplotype of O. platycephala (NC010199), followed by the Chinese Odontobutis yaluensis (85.83%, NC022818) (Figure 1). It is noteworthy that a high degree of genetic distance between the same species in Korea and further biogeographical study should be made to have a better understanding of the evolutional relationship within the genus Odontobutis. Figure 1. Phylogenetic relationship of Odontobutis platycephala in the order Perciformes. A phylogenetic tree was constructed with the currently reported complete mitogenomes in the order Perciformes by using the MEGA 7.0 software by Minimum Evolution (ME) algorithm with 1000 bootstrap replications. GenBank accession numbers and origin of the haplotypes were shown followed by each species scientific name. Disclosure statement The authors report that they have no conflicts of interest. The authors alone are responsible for the content and writing of the article. ==== Refs References Chae BS 1999 First record of odontobutid fish, Odontobutis obscura (Pisces, Gobioidei) from Korea . Kor J Ichthyol . 11 :12 –16 . Iwata A , Sakai H 2002 Odontobutis hikimius n. sp.: a new freshwater goby from Japan, with a key to species of the genus . Copeia . 2002 (1 ):104 –110 . Jun J , Choi SH , Kum JD 2016 Complete mitochondrial genome of the endemic South Korean species Odontobutis interrupta (Perciformes, Odontobutidae) . Mitochondr DNA A . 27 :2957 –2959 . Kearse M , Moir R , Wilson A , Stones-Havas S , Cheung M , Sturrock S , Buxton S , Cooper A , Markowitz S , Duran C , et al. 2012 Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data . Bioinformatics . 28 (12 ):1647 –1649 .22543367 Ki JS , Jung SO , Hwang DS , Lee YM , Lee JS 2008 Unusual mitochondrial genome structure of the freshwater goby Odontobutis platycephala: rearrangement of tRNAs and an additional non‐coding region . J Fish Biol . 73 (2 ):414 –428 . Kumar S , Stecher G , Tamura K 2016 MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets . Mol Biol Evol . 33 (7 ):1870 –1874 .27004904 Laslett D , Canbäck B 2008 ARWEN: a program to detect tRNA genes in metazoan mitochondrial nucleotide sequences . Bioinformatics . 24 (2 ):172 –175 .18033792