==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1687042 1687042 Version of RecordResearch Article Mitogenome Announcement The complete mitochondrial genome of Athalia proxima (Hymenoptera: Tenthredinidae) and phylogenetic analysis H. He et al.He Huimin a https://orcid.org/0000-0003-0505-7180Niu Gengyun a Zhang Bin b Wei Meicai a a College of Life Sciences, Jiangxi Normal University, Nanchang, China; b Key Laboratory for Cultivation and Protection of Non-Wood Forest Trees, Central South University of Forest and Technology, Changsha, China CONTACT Meicai Wei weimc@126.comCollege of Life Sciences, Jiangxi Normal University, Nanchang, China 8 11 2019 2019 4 2 3868 3869 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract The nearly complete mitochondrial genome of Athalia proxima is 15852 bp in size, containing 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs, and a partial control region. Compared to ancestral organization, seven tRNA genes were rearranged. Bayesian and maximum-likelihood trees recovered the monophyly of Tenthredinidae and A. proxima was the basal lineage. The mitogenome sequence provided fundamental data for resolving phylogenetic and genetic problems of Tenthredinidae. Keywords Mitogenomenext-generation sequencingphylogenyTenthredinidaeAthalia ==== Body Athalia proxima (Klug) is a common pest of Chinese cabbage of Brassicaceae with a wide distribution in East Asia. About 102 species and sub-species have been reported in Athalia (Taeger et al. 2010; Koch et al. 2015). The systematic position of Athalia within Tenthredinoidea is uncertain (Wei and Nie 1998; Schulmeister et al. 2002; Taeger et al. 2010). To obtain basic genetic information regarding A. proxima and enrich the Tenthredinoidea species genome resource to promote the phylogeny of Tenthredinoidea, we herein describe the near-complete mitogenome of A. proxima. Specimen (CSCS-Hym-MC0021) available at the Asia Sawfly Museum, Nanchang (ASMN) repository was collected from Mt. Dapan, Pan’an, Zhejiang (28.85°N 120.46°E) in 2015. Whole genomic DNA was extracted from the specimen’s thorax muscle using the DNeasyR Blood & Tissue Kits (Qiagen, Valencia, CA, USA). Genomic DNA was prepared in 150 bp paired-end libraries, tagged and subjected to the high-throughput Illumina Hiseq 4000 platform, yielding a total of 8,985,824 raw reads (SRP223539). DNA sequences were assembled using MitoZ (Meng et al. 2019), and Geneious Prime 2019.2.1 (https://www.geneious.com) using three relative species as reference with the mean depth of coverage across the sequences being 1718, 1981 and 2092, respectively. Annotations were generated in MITOS web server (Bernt et al. 2013) and revised in Geneious Prime when necessary. A nearly complete mitogenome of 15852 bp was obtained and deposited in GenBank with accession number MN527306. The trnP and trnT had altered positions, which were detected in Megalodontes. The ancestral pattern of trnI (+)-trnQ (−)-trnM (+) clusters were rearranged to trnC (+)-trnW (+)-trnQ (−)-trnM (+)-trnI (+), which is novel to the basal hymenopterans. The base composition was 43.7% (A), 11.1% (C), 7.3% (G) and 37.9% (T), with a high A + T content (80.4%) of PCGs, indicating significant A + T bias. The length of the PCGs accounted for 70.7% of the mitogenome cox1 originated from TTG. Five PCGs originated from ATG whilst another four originated from ATT. The remainder originated from ATA. The phylogenetic status of A. proxima was inferred using BA and ML topologies, performed for protein-coding. The phylograms produced identical topologies (Figure 1). Athalia proxima formed a sister clade with Tenthredinidae + Cimbicidae as one of the basal lineages of Tenthredinoidea. Figure 1. A majority-rule consensus tree inferred Bayesian inference using MrBayes v.3.2.2 (Ronquist et al. 2012) under the GTR + GAMMA model, based on the concentrated PCGs + RNA of 28 individuals of Symphytans, two Apocritans and four outgroups. GenBank accession numbers were provided with species names. DNA sequences (Total 11,393 bp) were aligned in TranslatorX (Abascal et al. 2010). Node numbers show Bayesian posterior probabilities (*=100) and bootstrap. Branch lengths represent the means of the posterior distributions. Correction Statement This article has been republished with minor changes. These changes do not impact the academic content of the article. Disclosure statement The authors have declared that no competing interests exist. ==== Refs References Abascal F , Zardoya R , Telford MJ 2010 TranslatorX: multiple alignment of nucleotide sequences guided by amino acid translations . Nucleic Acids Res . 38 (suppl_2 ):W7 –W13 .20435676 Bernt M , Donath A , Jühling F , Externbrink F , Florentz C , Fritzsch G , Pütz J , Middendorf M , Stadler PF 2013 MITOS: improved de novo metazoan mitochondrial genome annotation . Mol Phylogenet Evol . 69 (2 ):313 –319 .22982435 Koch F , Pauly A , Hora ZA , Boevé J-L 2015 Sawflies of Ethiopia (Hymenoptera: Argidae, Tenthredinidae) . Zootaxa . 4021 (1 ):119 –155 .26624122 Meng G , Li Y , Yang C , Liu S 2019 MitoZ: a toolkit for animal mitochondrial genome assembly, annotation and visualization . Nucleic Acids Res . 47 (11 ):e63 .30864657 Ronquist F , Teslenko M , Van Der Mark P , Ayres DL , Darling A , Höhna S , Larget B , Liu L , Suchard MA , Huelsenbeck JP 2012 MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space . Syst Biol . 61 (3 ):539 –542 .22357727 Schulmeister S , Wheeler WC , Carpenter JM 2002 Simultaneous analysis of the basal lineages of Hymenoptera (Insecta) using sensitivity analysis . Cladistics . 8 (5 ):455 –484 . Taeger A , Blank S , Liston A 2010 World catalog of Symphyta (Hymenoptera) . Zootaxa . 2580 (1 ):1 –1064 . Wei M , Nie H 1998 Generic list of Tenthredinoidea s. str.(Hymenoptera) in new systematic arrangement with synonyms and distribution data . J Cent South Forestry Coll . 18 (3 ):23 –31 .