==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1678423 1678423 Version of RecordResearch Article Mitogenome Announcement Assembly and phylogenetic analysis of the complete chloroplast genome sequence of Actinidia setosa H. Lin et al.Lin Haifeng ab Jiang Ling c Zhang Fuquan a Bai Di c a College of Information Science and Technology, Nanjing Forestry University, Nanjing, Jiangsu, China; b School of Civil Engineering, Nanjing Forestry University, Nanjing, Jiangsu, China; c College of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu, China CONTACT Di Bai baidi000@njau.edu.cnCollege of Engineering, Nanjing Agricultural University, Nanjing, Jiangsu, China 21 10 2019 2019 4 2 3679 3680 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract The complete chloroplast (cp) genome sequence of Actinidia setosa (A. setosa) was sequenced and assembled into a circular genome of 156,728 bp length using Illumina paired-end data. The cp genome composed of a pair of 24,090 bp inverted repeat (IR) regions separated by a large single copy region (LSC) of 88,256 bp and a small single copy region (SSC) of 20,292 bp. Additionally, a total of 131 genes, including 85 protein-coding genes, 38 tRNA genes and 8 rRNA genes, were identified in the A. setosa cp genome. Phylogenetic analysis showed that A. setosa was evolutionarily close to other two kiwifruits A. deliciosa and A. chinensis. Keywords Actinidia setosachloroplast genomephylogeny ==== Body Kiwifruit (genus Actinidia), also known as Chinese gooseberry, consisted of more than 70 species, which mainly distributed in East and Southeast Asia from Siberia to Sumatra (Chou et al. 2008; Hsieh et al. 2011). Kiwifruit is particularly rich in vitamin C and vitamin K, and having been given as medicine in traditional Chinese (Tang et al. 2019). Although kiwifruit enjoys worldwide popularity, its ambiguous genetic diversity and genetic differentiation have resulted in long-standing confusion of developing optimum conservation and management strategies. In this study, we report and characterize the complete cp genome sequence of A. setosa to provide important genomic resources for promoting its conservation and management. The leaf of A. setosa was collected from South China Botanical Garden, the Chinese Academy of Sciences (Geographic coordinate: 23°11′0.47″N, 113°21′37″E). The genomic DNA was extracted using the DNeasy plant Mini Kit (Quiagen, Carlsbad, CA, USA) and now stored in the Herbarium of South China Botanical Garden (accession number: 20160415AS01), Guangdong, China. Here, we assembled the complete cp genome sequence of A. setosa into a free-of-gap genome of 156,728 bp length using AbySS (Simpson et al. 2009), CD-Hit (Li and Godzik 2006), and Minimus2 (Sommer et al. 2007) from Illumina sequencing data, and annotated it using Plastid Genome Annotator (PGA) (Qu et al. 2019). The cp genome sequence was then submitted to GenBank under the accession number of MN326319. The complete cp genome of A. setosa consists of a LSC of 88,256 bp, a SSC of 20,292 bp and a pair of IR regions of 24,090 bp. The overall GC content of the whole cp genome is 37.2%, which is a very common value in Actinidia. The GC content of the IR regions was 42.84%, which is higher than that of LSC (35.51%) and SSC (31.18%). According the annotation results, a total of 121 genes were detected in A. setosa cp genome, including 85 protein-coding genes, 38 tRNA genes, and 8 rRNA genes. Eight tRNA genes, five protein-coding genes and all four rRNA genes were found to have two copies in the IR regions. Among the total 131 genes, 18 genes were found to contain one intron (7 tRNA genes: trnK-UUU, trnL-UAA, trnV-UAC, trnI-GAU × 2, and trnA-UGC × 2; 11 protein-coding genes: rps16, rpoC1, petB, petD, rpl16, rpl2, ndhB × 2, rps12×2, and ndhA), while only one gene contains two introns (ycf3). In order to clarify the position of A. setosa in the phylogenetic tree, we selected other 28 plant cp genomes with 76 conserved protein-coding genes to reconstruct a neighbor-joining (NJ) tree using MEGA6 with 1000 bootstrap replicates (Figure 1). The reconstructed NJ tree strongly support that A. setosa was evolutionarily close to other two kiwifruits A. deliciosa and A. chinensis. The complete cp genome sequence of A. setosa will provide important genomic resources to the conservation genetics of this species as well as for the evolution analysis of Actinidia. Figure 1. The neighbour-joining phylogenetic tree of 29 plant cp genomes based on 76 conserved genes. Bootstrap values are listed for each node. Accession numbers for tree reconstruction are listed right to their scientific names. Disclosure statement The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. ==== Refs References Chou HN , Nee CC , Ou ASM , Chou TH , Chien CC 2008 Characterization of the physico-chemical and antioxidant properties of Taiwanese kiwifruit (Actinidia setosa) . Botanical Stud . 49 :215 –224 . Hsieh TY , Ku SM , Chien CT , Liou YT 2011 Classifier modeling and numerical taxonomy of Actinidia (Actinidiaceae) in Taiwan . Botanical Stud . 52 :337 –357 . Li W , Godzik A 2006 Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences . Bioinformatics . 22 :1658 .16731699 Qu XJ , Moore MJ , Li DZ , Yi TS 2019 PGA: a software package for rapid, accurate, and flexible batch annotation of plastomes . Plant Methods . 15 :50 .31139240 Simpson JT , Wong K , Jackman SD , Schein JE , Jones SJ , Birol I 2009 ABySS: a parallel assembler for short read sequence data . Genome Res . 19 :1117 .19251739 Sommer DD , Delcher AL , Salzberg SL , Pop M 2007 Minimus: a fast, lightweight genome assembler . BMC Bioinformatics . 8 :64 –64 .17324286 Tang P , Shen RN , He RW , Yao XH 2019 The complete chloroplast genome sequence of Actinidia eriantha . Mitochondr DNA B . 4 :2114 –2115 .