==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1681320 1681320 Version of RecordResearch Article Mitogenome Announcement Characterisation of the complete mitochondrial genome of Bactrocera ruiliensis (Diptera: Tephritidae), with its phylogenetic analysis Y.-l. Ren et al.Ren Yan-Ling a Wang Tao ab Yang Maofa b Shao Weizhi c a Guizhou Light Industry Technical College, Guiyang, P. R. China; b Institute of Entomology, Guizhou University, Guiyang, P.R. China; c Ruili Customs Integrated Technology Center, Ruili, P. R. China CONTACT Tao Wang wjchuzhou@sina.comGuizhou Light Industry Technical College, Guiyang550025, P. R. China; Institute of Entomology, Guizhou University, Guiyang550025, P. R. China; Maofa Yang gdgdly@126.comInstitute of Entomology, Guizhou University, Guiyang550025, P. R. China 24 10 2019 2019 4 2 3750 3751 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract The complete mitochondrial genome (mitogenome) of the Bactrocera ruiliensis (Diptera: Tephritidae: Dacinae) is sequenced and annotated. The mitochondrial genome is 15,870 bp (GenBank No. MN477221) and has an A + T content of 73.3% (A 39.2%; C 16.2%; G 10.3%, and T 34.4%), which is the classical structure for insect mitogenome. All PCGs started with ATN, except ATP8, which is started with TTG; 12 PCGs use TAR (TAA/TAG) as the stop codon, except COX1, which ends with single T––. The phylogenetic tree confirms that B. ruiliensis clustered with other Bactrocera species. This study enriches the mitogenomes of the fruit flies. Keywords MitogenomeDacinaeBactrocera ruiliensisphylogeny ==== Body The genus Bactrocera Macquart belongs to Dacinae (Diptera: Tephritidae). At present, more 470 species of the genus are known from tropical, subtropical, and warm temperate regions of Asia, as well as in Australia and the South Pacific, with a few species distributed in southern Africa and the Hawaiian Islands (Wang et al. 2008; Yu et al. 2014). Some species of the genus are important quarantine pests, the identification of Bactrocera ruiliensis is mainly based on morphology and COI sequences, and no mitogenome data was available. Here, we have sequenced and determined the complete mitogenome of B. ruiliensis using next-generation sequencing. Total genome DNA was extracted from male adult of B. ruiliensis which was collected in Orchard, Huaxi District, Guiyang City, Guizhou province, China (E 106°37′11″, N 26°37′11″), in July 2018. The voucher of specimen and its genome DNA are deposited in the Institute of Entomology, Guizhou University, Guiyang, China (GUGC), accession number of them is GUGC-IDT-00125. These sequences were assembled using Geneious (v 10.2.3) (http://www.geneious.com/) (Kearse et al. 2012). Additionally, we used the MITOS server (http://mitos.bioinf.uni-leipzig.de/index.py) (Bernt et al. 2013) and tRNA scan-SE server (Lowe and Chan 2016) for annotation. The ML (maximum-likelihood) tree was constructed to investigate the molecular taxonomic position of B. ruiliensis using the nucleotide sequences of 13 protein-coding genes and two rRNA genes using IQ-TREE v1.6.3 (Nguyen et al. 2015). The complete mitogenome of B. ruiliensis is 15,870 bp (GenBank No. MN477221), containing 13 protein-coding genes (PCGs, 11,209 bp), 22 transfer RNA genes (tRNAs, 1467 bp), two ribosomal RNA genes (rRNAs, 2142 bp), and a large non-coding region (control region, 882 bp). The overall base composition of the genome is 39.2% A, 16.2% C, 10.3% G, and 34.4% T, exhibiting an obvious A + T bias (73.3%). The AT-skew (0.070) for the whole mitogenome is slightly positive while GC-skew (−0.223) is negative. All PCGs started with ATN (ATA/ATG/ATT/ATC), except ATP8, which is started with TTG; 12 PCGs use TAR (TAA/TAG) as the stop codon, except COX1, which ends with single T––. The phylogenetic relationships of B. ruiliensis were reconstructed with IQ-TREE using an ultrafast bootstrap approximation approach with 10,000 replicates based on concatenated the nucleotides of the 13 PCGs and two rRNAs with 13,197 bp (Figure 1). Each PCG and rRNA sequence was aligned using the MAFFT algorithm in TranslatorX and MAFFT v7.0 online serve with the G-INS-i strategy, respectively, and aligned sequences were eliminated using Gblocks 9.1 b (Abascal et al. 2010; Katoh et al. 2017). The phylogenetic tree confirms that B. ruiliensis as the sister group to B. dorsalis, B. invadens, B. papaya, B. philippinensis, and B. carambolae. Up to now, not a lots of studies have been recorded for Bactrocera, and we hope that our data can be useful for further study. Figure 1. Phylogenetic analyses of Bactrocera ruiliensis based upon the concatenated the nucleotides of the 13 PCGs and two rRNAs of 32 ingroup species using IQ-TREE. Numbers at nodes are bootstrap values. The accession number for each species is indicated after the scientific name. Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Abascal F , Zardoya R , Telford MJ 2010 Translator X: multiple alignment of nucleotide sequences guided by amino acid translations . 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