==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1678434 1678434 Version of RecordResearch Article Mitogenome Announcement The complete chloroplast genome sequence of Spiraea blumei G. Don (Rosaceae) Y. Huo et al.Huo Yan a Yan Ming b Zhao Xueqing b Zhu Zunling ac Yuan Zhaohe ab a College of Landscape Architecture, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China; b College of Forestry, Nanjing Forestry University, Nanjing, PR China; c College of Arts and Design, Nanjing Forestry University, Nanjing, PR China CONTACT Zunling Zhu zhuzunling@aliyun.comCollege of Landscape Architecture, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China; College of Arts & Design, Nanjing Forestry University, Nanjing, PR China; Zhaohe Yuan zhyuan88@hotmail.comCollege of Forestry, Nanjing Forestry University, Nanjing, PR China 21 10 2019 2019 4 2 3671 3672 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Spiraea blumei G. Don is an ornamental shrub widely distributed in Eastern Asia. Here, we reported and characterized the complete chloroplast (cp) genome sequence of S. blumei (GenBank accession number: MN418904) to provide genomic resources for promoting its conservation. The total chloroplast genome is 155,957 bp in length and contains the typical chloroplast structure, including two inverted repeat (IR) regions of 26,343 bp, a large single-copy (LSC) region of 84,384 bp, and a small single-copy (SSC) region of 18,887 bp. The overall guanine-cytosine (GC) content of the S. blumei chloroplast genome is 36.8%. The cp genome encodes 133 unique genes, including 85 protein-coding genes (PCGs), 40 tRNA genes, and 8 rRNA genes. We used the cp genome of S. blumei and 26 other cp genomes to perform a phylogenetic analysis, which indicated that S. blumei was closely related to S. martini in Rosaceae. Keywords Spiraea blumeicomplete chloroplast genomephylogenetic analysis ==== Body Spiraea blumei G. Don is a plant of the family Rosaceae and widely distributed in China, Japan, and Korea. It has high ornamental value for its white early summer flowers and elegant leaves (Zhang 2010). Besides, its leaves are a tea substitute (Kunkel 1984). So far, there have been no studies on the genome of S. blumei. Therefore, the genetic and genomic information is urgently needed to promote its conservation and systematics research. In this study, we sequenced the complete chloroplast (cp) genome of S. blumei based on an Illumina platform and analysed genetic relationships with the closely related species in Rosaceae. Experimental samples were collected from Nanjing, China (latitude: 32°07′86.1ʺN, longitude: 118°81′69.4ʺ E) and deposited at Nanjing Forestry University (accession number NFU19051103). Total DNA was extracted from fresh leaves of S. blumei individual by the modified method CTAB (Doyle 1987). Paired-end libraries were constructed and sequenced with an Illumina Hiseq 2500 platform (Nanjing, China) for paired-end 150 bp reads. In total, ca. 83.1 million high-quality clean reads were generated with adaptors trimmed. Then, NOVOPlasty3.1 (Dierckxsens et al. 2017) was used for de novo assembly with Prunus yedoensis (GenBank accession KU985054) as a reference. GeSeq (Tillich et al. 2017) was used for annotation and Geneious version 8.0.4 (Kearse et al. 2012) was used for inspecting and characterizing the chloroplast genome. The complete cp genome size of S. blumei is 155,957 bp in length, containing the large single copy (LSC, 84,384 bp), a small single copy (SSC, 18,887 bp), and two inverted repeats (IR, 26,343 pb) regions. The overall GC content of S. blumei cp genome is 36.8% and those in the LSC, SSC, and IR regions are 34.6%, 30.4%, and 42.5%, respectively. The cp genome encodes 133 unique genes, including 85 protein-coding genes (PCGs), 40 tRNA genes, and 8 rRNA genes. The tRNA genes are distributed throughout the whole genome with 25 in the LSC, 1 in the SSC, 14 in the IR regions, while rRNAs only situate in IR regions. Twenty-one genes have two copies, which include eight PCGs (ndhB, rpl2, rpl23, rps12, rps19, rps7, ycf1, ycf2), nine tRNA genes (trnA-UGC, trnI-CAU, trnI-GAU, trnL-CAA, trnM-CAU, trnN-GUU, trnR-ACG, trnT-GGU, trnV-GAC), and four all rRNA genes (rrn16, rrn23, rrn4.5, and rrn5). Among the PCGs, two genes (ycf3 and rps12) contain two introns, and 13 different genes (atpF, clpP, ndhA, ndhB, rpl2, rpoC1, rps16, trnA-UGC, trnG-UCC, trnI-GAU, trnK-UUU, trnL-UAA, trnV-UAC) have one intron each. The rps12 is a trans-spliced gene with 5′ end located in the LSC region and the duplicated 3′ end in the IR regions. Twenty-seven chloroplast genomes sequences were aligned with MAFFT version 7.3 (Katoh and Standley 2013), and the maximum likelihood (ML) inference was performed using TVM + F+R3 model with 1000 bootstrap replicates via IQ-TREE version.1.6.8 (Nguyen et al. 2015). The result revealed that S. blumei is closer to S. martini in Rosaceae (Figure 1). The complete chloroplast sequence of S. blumei will provide useful information for future studies to solve the problems related to the phylogeny of genus Spiraea and family Rosaceae. Figure 1. Maximum likelihood (ML) tree based on the chloroplast genome sequences of 27 species. Numbers on the nodes are bootstrap values from 1000 replicates. Elaeagnus macrophylla and Elaeagnus sp. were selected as outgroups. Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Dierckxsens N , Mardulyn P , Smits G 2017 NOVOPlasty: de novo assembly of organelle genomes from whole genome data . Nucleic Acids Res . 45 :e18 .28204566 Doyle JJ 1987 A rapid DNA isolation procedure for small quantities of fresh leaf tissue . Phytochem Bull . 19 :11 –15 . Katoh K , Standley DM 2013 MAFFT multiple sequence alignment software version 7: improvements in performance and usability . 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