==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1682475 1682475 Version of RecordResearch Article Mitogenome Announcement Complete mitogenome for the ber fruit fly, Carpomya vesuviana (Diptera: Tephritidae) from Toksun, Northwest China and its phylogenetic relationship within family Tephritidae P.-f. Jia et al.Jia Ping-Fan a Liu Jian-Hong a Xu Jin a Li Xiao-Jun b Dan Wen-Li a a Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming, China; b School of Nursury and Rehabillitation, Xinyu University, Xinyu, China CONTACT Jian-Hong Liu liujh@swfu.edu.cn; Jin Xu, xujin798@126.comKey Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming, China 5 11 2019 2019 4 2 3838 3839 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Carpomya vesuviana (Diptera: Tephritidae), commonly known as the ber fruit fly, is the most destructive insect pest of 'ber' (jujube) throughout Asia and Western Europe. Complete sequence of the mitogenome of C. vesuviana has been determined in this study. The circular genome is 15,267 bp long and contains a standard gene complement, that is, the large and small ribosomal RNA subunits, 22 transfer RNA genes, 13 genes encoding mitochondrial proteins, and a non-coding A + T-rich control region. The phylogeny showed that C. vesuviana of subfamily Trypetinae was monophyletic and clearly separated from both Dacinae and Tephritinae with maximum support (p = 1). Keywords Carpomya vesuvianamitochondrial genomemolecular phylogeny ==== Body Carpomya vesuviana, commonly known as the ber fruit fly, is the most destructive insect pest of ‘ber’ (jujube) throughout Asia and Western Europe (Azam et al. 2004). The fly is oligophagous and has been recorded on hosts from Ziziphus spp. fruits (Norrbom 1997). At present, there is only one mitochondrial genome data in subfamily Trypetinae of Family Tephritidae. In this study, we reported the complete mitogenome of C. vesuviana in subfamily Trypetinae. The results would greatly facilitate phylogenetics, population genetics, and species identification. The adult male flies were caught from Toksun (42.27°N, 88.09°E), Northwest China, on 13 August 2018. Specimen was deposited in the museum of Southwest Forestry University (Voucher Xinjiang 20180813), Kunming, China. Genomic DNA was extracted from adult fly following the manufacturer’s instruction in the DNeasy Blood and Tissue kit (Qiagen, Hilden, Germany) and then sequenced and assembled using Illumina’s HiSeq2000 platform (Illumina, San Diego, CA). The sequence was preliminarily aligned within the CLUSTAL X programme in BioEdit software. Protein-coding genes (PCGs), rRNA genes were predicted by using MITOS tools (Bernt et al. 2013), and tRNA was done through tRNAscan-SE (Lowe and Chan 2016). The complete mitogenome of C. vesuviana is 15,267 bp long in size (GenBank MN095236). The base-pair composition is 39.82% for A, 13.76% for C, 9.88% for G, and 36.54% for T. The mitogenome contains 13 PCGs, 22 transfer RNA genes, 2 ribosomal RNA genes, and a major non-coding region known as the CR (control region). J-strand codes nine PCGs (NAD2-3, NAD6, COX1-3, CYTB, ATP6, and ATP8) and 14 tRNAs, while N-strand codes four PCGs (NAD1, NAD4, NAD4l, and NAD5), eight tRNAs, and two rRNAs (16S rRNA and 12S rRNA). The gene arrangement of mitogenome is identical to the most common type of the putative ancestor of insects (Boore 1999; Cameron 2014). To validate the phylogenetic position, the mitogenomes of 20 species in family Tephritidae and the two outgroups, Drosophila suzukii and Drosophila melanogaster (Diptera: Drosophilidae) were clustered together to construct Bayesian tree by using the BI method in MrBayes version 3.2.6 (Huelsenbeck and Ronquist 2001; Dereeper et al. 2010, 2008). The result indicated that C. vesuviana in subfamily Trypetinae was monophyletic and clearly separated from both Dacinae and Tephritinae. In subfamily Dacinae, genus Dacus and Zeugodacus formed a sister clade to genus Bactrocera. The topology of the BI tree had maximum support (p = 1) (Figure 1). Furthermore, three subfamilies were correctly identified as assigned and monophyletic Dacinae, Trypetinae and Tephritinae (Figure 1). In conclusion, the mitochondrial genome of C. vesuviana reduced in the present study can provide essential DNA molecular data for further phylogenetic and evolutionary analysis. Figure 1. Molecular phylogeny for Carpomya vesuviana and the related 17 species in family Tephritidae and two outgroups based on complete mitogenome. Tree was constructed by Bayesian Inference (BI) method. Genbank accession numbers lie before the scientific name of species. The position of C. vesuviana is marked with solid square shape. Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Azam KM , Alansari MSA , Alraeesi AA 2004 Fruit flies of Oman with a new record of Carpomya vasuviana Costa (Diptera: Tephritidae) . Res Crops . 5 :274 –277 . Bernt M , Donath A , Juhling F , Externbrink F , Florentz C , Fritzsch G , Putz J , Middendorf M , Stadler PF 2013 MITOS: improved de novo metazoan mitochondrial genome annotation . Mol Phylogenet Evol . 69 (2 ):313 –319 .22982435 Boore JL 1999 Animal mitochondrial genomes . Nucleic Acids Res . 27 (8 ):1767 –1780 .10101183 Cameron SL 2014 Insect mitochondrial genomics: implications for evolution and phylogeny . Annu Rev Entomol . 59 (1 ):95 –117 .24160435 Dereeper A , Audic S , Claverie JM , Blanc G 2010 BLAST-EXPLORER helps you building datasets for phylogenetic analysis . BMC Evol Biol . 10 (1 ):8 .20067610 Dereeper A , Guignon V , Blanc G , Audic S , Buffet S , Chevenet F , Dufayard JF , Guindon S , Lefort V , Lescot M , et al. 2008 Phylogeny.Fr: robust phylogenetic analysis for the non-specialist . Nucleic Acids Res . 36 :W465 –9 .18424797 Huelsenbeck JP , Ronquist F 2001 MRBAYES: Bayesian inference of phylogenetic trees . Bioinformatics . 17 (8 ):754 –755 .11524383 Lowe TM , Chan PP 2016 tRNAscan-SE on-line: search and contextual analysis of transfer RNA genes . Nucl Acids Res . 44 :54 –57 . Norrbom AL 1997 The genus Carpomya Costa (Diptera: Tephritidae): new synonymy, description of first American species, and phylogenetic analysis . Proc Entomol Soc Washington . 99 :338 –347 .