==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1687028 1687028 Version of RecordResearch Article Mitogenome Announcement The complete mitochondrial genome of Chrysomya nigripes (Diptera: Calliphoridae) Z.-Y. Zhang et al.Zhang Ze-Ya a Ren Li-Pin b Leng Jing c Shang Yan-Jie b Zhu Guang-Hui a a Department of Forensic Medicine, Shantou University Medical College, Shantou, China; b Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha, China; c Cardiovascular research center, Shantou University Medical College, Shantou, China CONTACT Guang-Hui Zhu ghzhu@126.comDepartment of Forensic Medicine, Shantou University Medical College, Shantou, China 13 11 2019 2019 4 2 4012 4013 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Chrysomya nigripes (Diptera: Calliphoridae) is a blow fly species of forensic importance. Here we demonstrated the complete mitochondrial genome of this species for the first time. Phylogenetic analyses indicated that entire mitochondrial genome sequences can provide more useful information for distinguishing C. nigripes from the other species. Keywords Chrysomya nigripesmitochondrial genomespecies identificationphylogenetic analyses ==== Body Chrysomya nigripes (Diptera: Calliphoridae) (Aubertin, 1932) is a forensically important blow fly species, which is widespread in Asian and Australasian (O’Flynn 1983; Sukontason et al. 2005). Considering the underlying value in forensic entomology, here we report the entire mitochondrial genome sequence of C. nigripes for species identification and phylogenetic analysis (GenBank accession number: KT444441). Adult specimens of C. nigripes were collected in Changsha, China (28°9′N; 112°54′E) in August, 2014. All specimens were frozen to death and stored under −80 °C subsequently. The specimens were identified according to the key of morphological characteristics (Xue and Zhao 1996). The voucher specimens were assigned a unique field code with a sole code (STU20140801) and the DNA with a sole code (STU20140802) was deposited in the Zhu’s Lab (Shantou University, Shantou, China). The complete genome was amplified in eight fragments (Nelson et al. 2012), and the DNA fragments were sequenced on both strands by Sanger dideoxy sequencing method by a commercial service provider (Transduction Bio Co. Ltd. Wuhan, China). The entire genome of C. nigripes was circular with the length of 15,832 bp, including 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes and a control region. Phylogenetic analysis was conducted on 13 entire mitochondrial gene sequences from 9 species of Calliphoridae, together with another one from Muscidae family as the outgroup species. The phylogeny of family Calliphoridae based on the entire mitochondrial gene sequences was separated into several genetic clades (Figure 1). As the out group, two Muscidae samples were clustered together and definitely divided from the Calliphoridae. Figure 1. Neighbor-joining tree by maximum synthetic likelihood method based on the complete mitochondrial gene sequences from nine species of Calliphoridae. Here we demonstrated the complete mitochondrial genome of C. nigripes and performed the phylogenetic analysis. The results were considered to be valuabe for the implementation of the mitochondrial database of Calliphoridae, and for establishing methods for species identification of the fly group. Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Nelson LA , Lambkin CL , Batterham P , Wallman JF , Dowton M , Whiting MF , Yeates DK , Cameron SL 2012 Beyond barcoding: a mitochondrial genomics approach to molecular phylogenetics and diagnostics of blowflies (Diptera: Calliphoridae) . Gene . 511 (2 ):131 –142 .23043935 O’Flynn MA 1983 Notes on the biology of Chrysomya nigripes Aubertin (Diptera: Calliphoridae) . Aust J Entomol . 22 (4 ):341 –342 . Sukontason KL , Vogtsberger RC , Boonchu N , Chaiwong T , Sripakdee D , Ngern-Klun R , Piangjai S , Sukontason K 2005 Larval morphology of Chrysomya nigripes (Diptera: Calliphoridae), a fly species of forensic importance . J Med Entomol . 42 (3 ):233 –240 .15962769 Xue WQ , Zhao JM 1996 Flies of China . Shenyang : Liaoning Science and Technology Press .