==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1678421 1678421 Version of RecordResearch Article Mitogenome Announcement Complete mitochondrial genome of Accipiter trivirgatus F. Zhang et al.Zhang Fengling abc Zhou Lizhi abc Dong Yuanqiu abc Song Yunwei d a School of Resources and Environmental Engineering, Institute of Biodiversity and Wetland Ecology, Anhui University, Hefei, China; b Anhui Province Key Laboratory of Wetland Ecosystem Protection and Restoration, Anhui University, Hefei, China; c Anhui Biodiversity Information Center, Anhui University, Hefei, China; d Shengjin Lake National Nature Reserve of Anhui Province, Dongzhi, China CONTACT Lizhi Zhou zhoulz@ahu.edu.cnSchool of Resources and Environmental Engineering, Anhui University, 111 Jiulong Road, Hefei230601, China 18 10 2019 2019 4 2 3652 3653 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Crested goshawk (Accipiter trivirgatus) is a diurnal raptor tropical Asia which is a bird species in family Accipitridae. In the present study, we determined its complete mitochondrial genome by PCR-based method. The complete mitochondrial genome was 18,454 bp in length which overall base composition was 31.2% A, 24.4% T, 31.0% C, and 13.4% G. It consisted of the typical structure of 13 PCGs, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 2 control regions. All of the PCGs started with ATG codon, except for ND3 which was started with ATC. Most of the genes terminate with codons TAA. The non-coding regions include pseudo-control regions. Keywords Accipiter trivirgatusmitochondrial genomegene organizationpseudo-control regions ==== Body Crested goshawk (Accipiter trivirgatus) is a bird species in family Accipitridae which is a diurnal raptor in tropical Asia. There are 11 subspecies for Accipiter trivirgatus complex. Two of them distribute in China, one is A. t. indicus in S China and the other is A. t. formosae in Taiwan (Dickinson, 2003). Up to date, only partial mitochondrial sequence for Accipiter trivirgatus is available. In the present study, we amplified and sequenced the complete mitochondrial DNA of the Accipiter trivirgatus for the first time with the hope to provide the basic mitogenome data for future phylogenetic studies of Accipitridae birds. The muscle sample was collected from a wild Accipiter trivirgatus that died of natural causes by accident in Shengjin Lake National Nature Reserve (117° 3′32.59″E, 30°22′48.85″N), Anhui Province, China on January 16, 2019. The specimen is stored at Shengjin Lake Station for Wetland Ecosystem Research (Sample code is SJL20190116). The complete mitogenome sequence of Accipiter trivirgatus was amplified and sequenced by the normal LA-PCR and PCR-based methods. The DNA sequence has been deposited in GenBank with accession number MK953813. The complete mitogenome Accipiter trivirgatus was an 18,454 bp circular DNA molecule. The overall nucleotide composition was: 31.2% A, 24.4% T, 31.0% C, and 13.4% G, with a total A + T content of 54.6%.The mitogenome included 13 protein-coding genes, 22 tRNA and 2 rRNA genes, and 2 AT-rich control regions. Of the 13 protein-coding genes (PCGs), the longest one was ND5 (1817 bp), and the shortest was ATP8 (167 bp), similar to the other Accipiter species (Song et al. 2015). All of the PCGs started with ATG codon, except for ND3 which was started with ATC. Most of the genes terminated with codons TAA, ND1 and COXI with AGG, ND5 terminated with AGA.COXIII and ND4 genes had an incomplete termination codon “T—”, which was the 5’ terminal of the adjacent gene. The 12S rRNA was 971 bp long and the 16S rRNA was 1610 bp in length. In addition, the non-coding regions included 2 control regions (D-loop).The first D-loop was 1233 bp, located between tRNAThr and tRNAPro, and secondary D-loop was 1688 bp, located between tRNAGlu and tRNAPhe. Phylogenetic analyses were conducted with mitochondrial genomic data of 14 avian species (including the Accipiter trivirgatus) from the GenBank database. The topology of the tree inferred using Neighbour-Joining methods in the programme MEGA 5.05, and bootstrap analysis was performed with 100 replications (Breman et al. 2013). The phylogenetic tree showed that all Accipiter species had a close phylogenetic relationship. Accipiter trivirgatus, Accipiter gentilis, Accipiter nisus, Accipiter soloensis, Accipiter virgatus in one clade, and Accipiter trivirgatus had relatively distant systematic relationship to the other four Accipiter species (Figure 1). Figure 1. Phylogenetic analysis based on complete mitochondrial genome sequences. An N-J tree were built based on the phylogenetic analysis of 13 Falconiformes species’ complete mitochondrial genomes and Gallus gallus used as an outgroup. The mitochondrial genome sequences of analyzed species were obtained from the GenBank databases. Disclosure statement The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of the paper. ==== Refs References Breman FC , Jordaens K , Sonet G , Nagy ZT , Van Houdt J , Louette M 2013 DNA barcoding and evolutionary relationships in Accipiter Brisson, 1760 (Aves, Falconiformes: Accipitridae) with a focus on African and Eurasian representatives . J Ornithol . 154 :265 –287 . Dickinsion EC , editor. 2003 The Howard and Moore complete checklist of the birds of the world . 3rd ed. Princeton, New Jersey : Princeton University Press . Song X , Huang J , Yan C , Xu G , Zhang X , Yue B 2015 The complete mitochondrial genome of Accipiter virgatus and evolutionary history of the pseudo-control regions in Falconiformes . Biochem Systemat Ecol . 58 :75 –84 .