==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1673241 1673241 Version of RecordResearch Article Mitogenome Announcement The complete chloroplast genome of Ageratum conyzoides (Asteraceae) Z.-P. Qiao et al.Qiao Zi-Peng Chen Zhi-Xiang Wang Qi-Zhi College of Chemical Engineering, Huaqiao University, Xiamen, PR China CONTACT Qi-Zhi Wang wqz@hqu.edu.cnCollege of Chemical Engineering, Huaqiao University, Xiamen361021, PR China 4 10 2019 2019 4 2 3342 3343 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Ageratum conyzoides L. is an important Chinese medicinal plant. In this study, we reported the complete chloroplast genome of A. conyzoides. The chloroplast genome sequence is 151,309 bp in length and consisted of a large single copy (LSC) region (83,884 bp), a small single copy (SSC) region (17,771 bp), and two inverted repeats (IRs) (24,827 bp). It was composed of 126 genes and they were 81 protein-coding genes, 30 tRNA genes, 8 rRNA genes, and 7 pseudogene. Phylogenetic analysis with reported chloroplast genomes can not only show that A. conyzoides has a close genetic relationship with Centaurea diffusa and Carthamus tinctorius, but also provide new evidence for the identification of Praxelis clematidea and A. conyzoides. Keywords Ageratum conyzoides L.complete chloroplast genomephylogenetic analysis ==== Body Ageratum conyzoides L., is an annual herb with a long history of traditional medicinal uses in many countries in the world, especially in the tropical and subtropical regions (Okunade 2002). The extracts and metabolites of A. conyzoides have been used as a bacteriocide, antidysentric, anti-diabetic, insecticide, and herbicide (Borthakur and Baruah 1987; Xuan et al. 2004; Nogueira et al. 2010). However, due to its purple floret, A. conyzoides is sometimes difficult to distinguish from other purple floret plant, such as Praxelis clematidea. As such, obtaining the complete chloroplast sequence will enable identification by molecular markers. Fresh leaves of A. conyzoides collected from Huaqiao University (31°15N, 109°56E), Fujian Province, China. Voucher specimens were deposited in Huaqiao University Herbarium (18014011). Total genomic DNA was extracted by PlantGenomic DNA Kit (Sangon Biotech, Shanghai, China). Paired-end reads were sequenced by using Illumina Hiseq Platform (Illumina, San Diego, CA). Approximately 10 Gb of paired-end (150 bp) sequence data were randomly extracted from the total sequencing output and used as input for NOVOPlasty (Dierckxsens et al. 2017) to assemble the plastid genome. The plastid genome of P. clematidea (GenBank accession number: NC_023833.1) was used as the seed sequence. The assembled sequence was annotated using Geneious version 11.0.4 (Kearse et al. 2012) by comparing it with the complete chloroplast genomes of P. clematidea. At last, the annotated chloroplast genome sequence was submitted to GenBank with the accession number MK905238. The A. conyzoides chloroplast genome was 151,309 bp in length with an overall GC content of 37.4%. The circle genome was comprised a large single copy (LSC) region (83,884 bp), a small single copy (SSC) region (17,771 bp), and two inverted repeats (IRs) (24,827 bp). The complete chloroplast genome contains 126 genes, including 81 protein-coding genes, 30 tRNA genes, 8 rRNA, genes, and 7 pseudogene. A neighbour-joining (NJ) tree was performed with EGA7.0 (Kumar et al. 2016) based on 13 complete chloroplast genome sequences of Asteraceae and Platycodon grandiflorus (Campanulaceae) (NC 035624) as an outgroup using 1000 bootstrap replicates (Figure 1). The tree showed a close relationship between Centaurea diffusa and Carthamus tinctorius. This complete cp genome can not only be further used for population genomic studies, phylogenetic analyses and genetic engineering studies of Asteraceae, but also provide new evidence for the identification of P. clematidea and A. conyzoides. Figure 1. Neighbour-joining (NJ) phylogenetic tree based on 14 complete chloroplast genomes. Accession numbers: Ageratum conyzoides (MK905238); Aster spathulifolius (NC_027434); Centaurea diffusa (NC_024286); Carthamus tinctorius (NC_030783); Silybum marianum (NC_028027); Praxelis clematidea (KF922320); Ageratina adenophora (NC_015621); Guizotia abyssinica (NC_010601); Helianthus annuus (NC_007977); Parthenium argentatum (NC_013553); Chrysanthemum indicum (JN867589); Leontopodium leiolepis (NC_027835); and Platycodon grandiflorus (NC_035624). Acknowledgement The authors thank Professor Yan Yu (College of Life Sciences, Sichuan University) and Huang min for the help of sequence analysis. Disclosure statement The authors declare no conflicts of interest and are responsible for the content. ==== Refs References Borthakur N , Baruah AKS 1987 Search for precocenes in Ageratum conyzoides Linn. of North-East India . J Indian Chem Soc . 64 :580 –581 . Dierckxsens N , Mardulyn P , Smits G 2017 . NOVOPlasty: de novo assembly of organelle genomes from whole genome data. Nucleic Acids Res. 45:e18. Kearse M , Moir R , Wilson A , Stones-Havas S , Cheung M , Sturrock S , Buxton S , Cooper A , Markowitz S , Duran C 2012 Geneious basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data . Bioinformatics . 28 :1647 –1649 .22543367 Kumar S , Stecher G , Tamura K 2016 MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets . 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