==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1670108 1670108 Version of RecordResearch Article Mitogenome Announcement Complete mitochondrial genome of the deep pugnose ponyfish Secutor ruconius (Perciformes: Leiognathidae) in the East China Sea Y. Sui et al.https://orcid.org/0000-0003-2273-8367Sui Yanming Qin Bo Song Xuefeng Sheng Wenquan Zhang Bianbian Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture; East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China CONTACT Bianbian Zhang zhangbianbian@hotmail.comKey Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture; East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai200090, China 15 10 2019 2019 4 2 3563 3564 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract The complete mitochondrial genome sequence of Secutor ruconius is firstly described in this article. The total length of mitogenome is16,465 bp. It contains 13 protein-coding genes, 22 tRNA genes, and 2 ribosomal RNA genes. The overall base composition of H-strand is 31.58% A, 28.65% C, 25.23% T, and 14.53% G, with an A + T bias of 56.81%. The phylogenetic analysis result showed that the S. ruconius, Zebrasoma flavescens, and Pristipomoides multidens were closely related. Keywords Secutor ruconiusmitochondrial genomephylogenetic analysis ==== Body The deep pugnose ponyfish Secutor ruconius belongs to genus Secutor in family Leiognathidae of Chaetodontiformes. As a schooling demersal omnivore, S. ruconius plays an important role in reflecting any effects of harbor building activities. S. ruconius is one of the dominant benthic species in the North Bay of New Caledonia and Pagbilao of Philippines (Pinto 1988; Wantiez et al. 1996). The complete mitochondrial genome of S. ruconius first determined in this paper was expected to provide help on population genetics of S. ruconius and further molecular phylogenetic studies. The sample of S. ruconius in this article was collected from the East China Sea (123°56′E and 33°52′N). And the specimen is stored in the Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture. According to genes from S. ruconius, such as 12S ribosomal RNA gene (Accession: KY352498), 16S ribosomal RNA gene (Accession: EU741807), cytochrome c oxidase subunit I (COI) gene (Accession: EF609613), primers were designed, and PCR amplification and sequencing were conducted. The whole length of S. ruconius mitogenome was 16,465 bp and submitted in GenBank (Accession No. MN251862). The nucleotide composition of the heavy strand was 31.58% for A, 28.65% for C, 25.23%for T, and 14.53% for G, with a high A + T bias of 56.81%. It contains 13 protein-coding genes, 22 tRNAs and 2 rRNAs. Most genes were located on the heavy strand, but ND6 and 8 tRNA genes (tRNAGln, RNAAla, tRNAAsn, tRNACys,tRNATyr, tRNASer, tRNAGlu, tRNAPro) were encoded on the light strand. Most protein-coding genes initiated with ATG except for COI starting with GTG. It is also important to note that the majority of protein-coding genes (7 of 13 genes) is inferred to terminate with an incomplete stop codon T or TA– (ND2, COII, ATPase 6, COIII, ND3, ND4, and Cyt b); five protein-coding genes share the typical termination codon TAA (ND1, COI, ATPase 8, ND4L, and ND5); ND6 uses TAG as a stop codon. The length of 12S (located between tRNAPheand tRNAVal) and 16S (located between tRNAVal and tRNALeu) rRNA genes were 950 bp and 1692 bp, respectively. To investigate the phylogenetic relationship, we downloaded the mitochondrial genome sequences of 16 currently available Eupercaria. The concatenated sequences of 13 protein-coding genes, 2 rRNAs genes, and 22 tRNAs genes were aligned with the ClustalW program (Larkin et al. 2007). Using the maximum-likelihood (ML) method (Stamatakis 2006), the phylogenetic tree was constructed (Figure 1) by MEGA6 (Tamura et al. 2013). The best-fitting model (GTR + I + G) was obtained as the optimization model by jModelTest (Posada 2008). The result indicating that the S. ruconius, Zebrasoma flavescens, and Pristipomoides multidens were closely related (Figure 1). Figure 1. The phylogenetic tree based on the 13 protein-coding genes, 2 rRNAs genes, and 22 tRNAs genes of Secutor ruconius, Zebrasoma flavescens, Pristipomoides multidens, Carangoides equula, Macquaria australasica, Oplegnathus fasciatus, Photopectoralis bindus, Gazza minuta, Nuchequula nuchalis, Siganus guttatus, Lutjanus rivulatus, Paracanthurus hepatus, Naso lopezi, Scombrops gilberti, Platax teira, Plectorhinchus lineatus, Triacanthodes anomalus and an outgroup Protopterus annectens. The bootstrap (1000 replicates test) supports for maximum-likelihood (ML) method is indicated at each branch. Correction Statement This article has been republished with minor changes. These changes do not impact the academic content of the article. Disclosure statement The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper. ==== Refs References Larkin MA , Blackshields G , Brown NP , Chenna R , McGettigan PA , McWilliam H , Valentin F , Wallace IM , Wilm A , Lopez R , et al. 2007 Clustal W and Clustal X version 2.0 . Bioinformatics . 23 (21 ):2947 –2948 .17846036 Pinto L 1988 Population dynamics and community structure of fish in the mangroves of Pagbilao, Philippines . J Fish Biol . 33 :35 –43 . Posada D 2008 jModelTest: phylogenetic model averaging . Mol Biol Evol . 25 (7 ):1253 –1256 .18397919 Stamatakis A 2006 RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models . Bioinformatics . 22 (21 ):2688 –2690 .16928733 Tamura K , Stecher G , Peterson D , Filipski A , Kumar S 2013 MEGA6: Molecular Evolutionary Genetics Analysis version 6.0 . 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