==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1673253 1673253 Version of RecordResearch Article Mitogenome Announcement Complete mitochondrial genome sequence of nanday conure, Aratinga nenday (Psittaciformes: Psittacidae) D. W. Liu et al.Mitochondrial Dna Part B: ResourcesLiu Da-Wei abc Pan Yang d Zhou Yong-Wu abc Hou Sen-Lin abc a Nanjing Forest Police College, Nanjing, PR China; b Forensic Identification Center for Forest Police of the State Forest Bureau, Nanjing, PR China; c Key Laboratory of State Forest and Grassland Administration Wildlife Evidence Technology, Nanjing, PR China; d Ministry of Ecology and Environment, Nanjing Institute of Environmental Sciences, Nanjing, PR China CONTACT Da-Wei Liu 272756332@qq.comNanjing Forest Police College, Nanjing, PR China 4 10 2019 2019 4 2 3348 3349 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Here, we report the complete mitochondrial genome of Aratinga nenday. It was found to be a closed circular molecule of 16,983 bp, containing 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes, and 1 non-coding control region. The overall base composition was 54.03% A + T and 45.973% G + C contents. The complete mtDNA of A. nenday and other 14 species were subjected to phylogenetic analysis by the neighbour-joining method and the Kimura 2-parameter model, which showed A. nenday to be a sister to the congeneric Aratinga solstitialis. Keywords Nanday conuremitochondrial genomeAratinga nenday ==== Body Nanday conure (Aratinga nenday) belongs to the family Psittacidae (Aves, Psittaciformes). This species is native to Argentina, Bolivia, Brazil, and Paraguay (Forshaw 2010), and inhabits forest and savanna (IUCN: Red List of Threatened Species 2019). Although this species is believed to have increased in population because of the creation of new suitable habitat areas owing to the ongoing habitat degradation (BirdLife International 2019), A. nenday was listed on CITES Appendix II in view of the associated wild animal trade. In this study, we characterized and described the mitogenome of A. nenday to obtain basic genetic information of this species. A feather sample was collected from Shangqiu Zoo (34.46N°, 115.67E°) in Shangqiu, Henan Province, China. The voucher specimen (A-2019002) was stored in the Key Laboratory of Wildlife Evidence Technology State Forest and Grassland Administration. Genomic DNA was isolated using a kit (Takara, Beijing, China), and a set of primers was designed for PCR amplification. The amplified products were subjected to Sanger sequencing in order to obtain genome information. The complete mitochondrial genome of A. nenday was found to be a typical circular molecule composed of 16,983 bp (GenBank accession No. MK965540). It comprised 22 transfer RNA genes, 13 protein-coding genes, 2 ribosomal RNA genes (rrnL, rrnS), and 1 non-coding control region (D-loop). The gene order and organization of A. nenday was identical to that of other parrots (Eberhard and Wright 2016; Liu et al. 2019). The overall base composition of the mitogenome was relatively similar, with A + T content being 54.03% (A, G, C, and T was 30.30%, 14.03%, 31.94%, 23.74%, respectively). The complete mitochondrial genome of A. nenday was then subjected to phylogenetic analysis along with that of 14 other parrot species recorded in the GenBank. A phylogenetic tree was constructed using the neighbour-joining method with 1000 bootstrap replicates and the Kimura 2-parameter model in MEGA 7.0 (Kumar et al. 2016). As a result, A. nenday was placed as sister to Aratinga solstitialis of the same genus (Figure 1). The Aratinga parrots were also closely related to Ara severus (Figure 1), which is distributed in Panama, Colombia, Brazil, and Peru (Forshaw 2010). This is the first report of the complete mitochondrial DNA of A. nenday, and the molecular data obtained could contribute to the future examination of evolutionary diversification of conures. Figure 1. Neighbour-joining phylogenetic tree based on the complete mitogenomes of A. nenday and other 14 parrot species using MEGA 7.0. Disclosure statement The authors report no conflicts of interest. The sequence has been submitted to NCBI under the accession no. MK965540. ==== Refs References BirdLife International. 2019 Species factsheet: Aratinga nenday . Cambridge (UK) : BirdLife International ; [accessed 2019 Aug 18]. http://www.birdlife.org. Eberhard JR , Wright TF 2016 Rearrangement and evolution of mitochondrial genomes in parrots . Mol Phylogenet Evol . 94 :34 –46 .26291569 Forshaw JM 2010 Parrots of the world . London (UK) : A & C Black Publishers Ltd . IUCN: Red List of Threatened Species. 2019 Nanday parakeet . Brussels (Belgium) : International Union for Conservation of Nature [accessed 2019 Aug 18]. http://www.iucnredlist.org. Kumar S , Stecher G , Tamura K 2016 MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets . Mol Biol Evol . 33 :1870 –1874 .27004904 Liu HY , Ding YF , Chen R , Tong Q , Chen PX 2019 Complete mitogenomes of two Psittacula species, P. derbiana and P. Eupatria. Mitochondrial DNA B . 4 :692 –693 .