==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1674703 1674703 Version of RecordResearch Article Mitogenome Announcement Characterisation of the complete mitochondrial genome of Lucanus chengyuani (Coleoptera: Lucanidae) L.-j. Wang et al.Mitochondrial Dna Part BWang Liang-Jong a Huang Jen-Pan b Shiao Shiuh-Feng c Ko Hsin-Ping d Sung Chia-Hsuan e a Division of Forest Protection, Taiwan Forestry Research Institute, Taipei, Taiwan; b Biodiversity Research Center, Academia Sinica, Taipei, Taiwan; c Department of Entomology, National Taiwan University, Taipei, Taiwan; d Taiwan Insect Museum, Taipei, Taiwan; e Planning and Information Division, Fisheries Research Institute, Keelung, Taiwan CONTACT Chia-Hsuan Sung chsung.tfrin@gmail.comPlanning and Information Division, Fisheries Research Institute, 199, Hou-I Road, Keelung 202, Keelung, Taiwan 12 10 2019 2019 4 2 3460 3462 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract We sequenced and assembled the complete mitochondrial genome of Lucanus chengyuani, from the Alishan, Chiayi County, Taiwan. The length of the complete mitogenome of L. chengyuani is 16,926 bp and the mitogenome contains 13 protein-coding, 22 tRNA, and 2 rDNA genes. Nucleotide compositions of the whole mitogenome of L. chengyuani are 38.37% for A, 27.96% for T, 23.03% for C, and 10.637% for G. The AT and GC skewness of mitogenome sequence are 0.157 and -0.368, showing the A-skew and C-skew. The reconstructed phylogenetic relationships of 9 Lucanidae species based on 13 mitochondrial protein-coding genes are highly supported. The clade including Neolucanus maximus and Odontolabis cuvera is sister to the rest of the stag beetle clades, which contains L. chengyuani and L. mazama. Mitogenomic data from this study will provide useful information for further studies for the population genetics, speciation, biogeography, and conservation of L. chengyuani in the future. Keywords Mitochondrial genomeLucanus chengyuaniLucanidaenext-generation sequencing ==== Body Stag beetles are popular insects and draw much attention from stag beetle enthusiasts and taxonomists because of their charismatic male mandibles. The Lucanidae is a diverse family distributed worldwide with about 110 genera and about 1400 described species (Holloway 2007; Fujita 2010). Lucanus chengyuani was recently described endemic species to Taiwan (Wang and Ko 2018). It is the third species that has a diurnal mate-searching flight behaviour in the genus Lucanus in Taiwan. The habitat of L. chengyuani is the open area along forest edges with miscellaneous trees and herbaceous plants. The small body size of L. chengyuani likely represents an evolutionary consequence of feeding on low-nutritional food in the habitat (Wang and Ko 2018). This is the first report of a complete mitochondrial genome sequence for the species Lucanus chengyuani. In this study, a single specimen (paraype no. Lcy009) of L. chengyuani was collected in Alishan, Chiayi County, Taiwan in May 2018. Total genomic DNA was extracted from the thorax using the QuickExtract™ DNA Extraction Solution kit (Epicentre, Madison, WI, USA) following the supplier’s instructions. The voucher specimen (paraype no. Lcy009) was deposited in Taiwan Insect Museum, Taipei, Taiwan and the voucher specimen’s genomic DNA was deposited in the Taiwan Forestry Research Institute, Taipei, Taiwan. The complete mitogenome of L. chengyuani was sequenced using the next-generation sequencing method (Illumina MiSeq, San Diego, CA, USA) (Hahn et al. 2013). A total of 12 Gb next-generation sequencing paired-end reads were used to assemble the complete mitogenome sequence. The CLC Genomics Workbench (QIAGEN) was used for sequence quality analysis, data trimming, and de novo assembling. The locations of the protein-coding genes, ribosomal RNAs (rRNAs), and transfer RNAs (tRNAs) were predicted by using MITOS Web Server (Bernt et al. 2013) and identified by alignment with other mitogenomes of Lucanidae. The AT and GC skew were calculated according to the following formulas: AT skew = (A–T)/(A + T) and GC skew = (G–C)/(G + C) (Perna and Kocher 1995). The complete mitogenome of L. chengyuani is 16,926 bp in length (GenBank Accession No. MK878514), including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 control region. The nucleotide compositions of the L. chengyuani mitogenome were 38.37% for A, 27.96% for T, 23.03% for C, and 10.637% for G. The AT and GC skewness of mitogenome sequence were 0.157 and -0.368 showing the A-skew and C-skew. The gene rearrangement of the mitogenome in L. chengyuani is identical to the inferred ancestral insect type (Cameron 2014). We reconstructed the phylogenetic relationships of Scarabaeoidea including 9 Lucanid species and 5 Scarabaeid species, and Xylosandrus crassiusculus (Curculionidae) as outgroup using Mrbayes v. 3.2.4 (Qiagen, Shanghai, China) (Huelsenbeck and Ronquist 2001) under the molecular evolutionary model GTR + I+G (Figure 1). Nodal supports were indicated by posterior probabilities. The clade including L. chengyuani and the other eight Lucanid species received absolute support (100%). The clade including Neolucanus maximus and Odontolabis cuvera is sister to all the other stag beetle clades, which contain L. chengyuani and L. mazama. Our result of phylogenetic reconstruction is not consistent with the result from a previous study of Kim and Farrell (2015). More complete mitogenomic data from other Lucanid species are needed for further studies on the phylogeny of Lucanidae. Mitogenomic data from this study will provide useful information for further studies for the population genetics, speciation, biogeography, and conservation of L. chengyuani in the future. Figure 1. Phylogenetic tree of the 9 Lucanid species, 5 Scarabaeid species, and 1 Curculionid species based on the sequences of 13 mitochondrial protein-coding genes. The phylogenetic tree was inferred with Mrbayes v. 3.2.4 (Huelsenbeck and Ronquist 2001) under model GTR + I + G. Value on nodes indicated posterior probabilities. Cheirotonus jansoni (KC428100) (Shao et al. 2014), Dichotomius schiffleri (KY100258) (Amorim et al. 2017), Dynodorcus curvidens (MF612067) (Chen et al. 2018), Lucanus chengyuani (MK878514, in this study), Lucanus mazama (FJ613419) (Sheffield et al. 2009), Macrodorcas seguyi (MF612068) (Chen et al. 2018), Neolucanus maximus (MF401425), Odontolabis cuvera (MF908524), Osmoderma opicum (KU500641), Popillia japonica (MG971231) (Yang et al. 2018), Prosopocoilus gracilis (KP735805), Prosopocoilus astacoides (KF364622) (Kim et al. 2015), Prosopocoilus confucius (KU552119) (Lin et al. 2017), Protaetia brevitarsis (KC775706) (Kim et al. 2014), Xylosandrus crassiusculus (KX035196). Acknowledgements We are grateful to Hsieh Jui-Fan, Amy Tsai and Jer-Wei Chang for their assistance in DNA data downloading. Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Amorim IC , Melo ADS , Cruz GADS , Wallau GDL , Moura RDCD 2017 Dichotomius (Luederwaldtinia) schiffleri (Coleoptera: Scarabaeidae) mitochondrial genome and phylogenetic relationships within the superfamily Scarabaeoidea . Mitochondr DNA B . 2 (2 ):887 –888 . Bernt M , Donath A , Jühling F , Externbrink F , Florentz C , Fritzsch G , Pütz J , Middendorf M , Stadler PF 2013 MITOS: improved de novo metazoan mitochondrial genome annotation . Mol Phylogenet Evol . 69 (2 ):313 –319 .22982435 Cameron SL 2014 Insect mitochondrial genomics: Implications for evolution and phylogeny . Annu Rev Entomol . 59 (1 ):95 –117 .24160435 Chen YJ , Liu J , Cao YY , Zhou S , Wan X 2018 Two new complete mitochondrial genomes of Dorcus stag beetles (Coleoptera, Lucanidae) . Genes Genom . 40 (8 ):873 –880 . Fujita H 2010 The Lucanid beetles of the world. Mushi-sha’s iconogrpahic series of insects 6 . Tokyo : Mushi-sha . Hahn C , Bachmann L , Chevreux B 2013 Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads-a baiting and iterative mapping approach . Nucleic Acids Res . 41 (13 ):e129 .23661685 Holloway BA 2007 Lucanidae (Insecta: Coleoptera). Fauna of New Zealand 61 . Lincoln : Manaaki Whenua Press . Huelsenbeck JP , Ronquist F 2001 MRBAYES: Bayesian inference of phylogenetic trees . Bioinformatics . 17 (8 ):754 –755 .11524383 Kim MJ , Im HH , Lee KY , Han YS , Kim I 2014 Complete mitochondrial genome of the whiter-spotted flower chafer, Protaetia brevitarsis (Coleoptera: Scarabaeidae) . Mitochondr DNA . 25 (3 ):177 –178 . Kim MJ , Kim KG , Kim SR , Kim I 2015 Complete mitochondrial genome of the two-spotted stag beetle, Metopodontus blanchardi (Coleoptera: Lucanidae) . Mitochondr DNA . 26 (2 ):307 –209 . Kim SI , Farrell BD 2015 Phylogeny of world stag beetles (Coleoptera: Lucanidae) reveals a Gondwanan origin of Darwin’s stag beetle . Mol Phylogenet Evol . 86 :35 –48 .25732069 Lin ZQ , Song F , Li T , Wu YY , Wan X 2017 New mitogenomes of two Chinese stag beetles (Coleoptera, Lucanidae) and their implications for systematics . J Insect Sci . 17 (2 ):63 . Perna NT , Kocher TD 1995 Patterns of nucleotide composition at fourfold degenerate sites of animal mitochondrial genomes . J Mol Evol . 41 (3 ):353 –358 .7563121 Shao LL , Huang DY , Sun XY , Hao JS , Cheng CH , Zhang W , Yang Q 2014 Complete mitochondrial genome sequence of Cheirotonus jansoni (Coleoptera: Scarabaeidae) . Genet Mol Res . 13 (1 ):1047 –1058 .24634126 Sheffield NC , Song H , Cameron SL , Whiting MF 2009 Nonstationary evolution and compositional heterogeneity in beetle mitochondrial phylogenomics . Syst Biol . 58 (4 ):381 –394 .20525592 Wang LJ , Ko HP 2018 Description of Lucanus chengyuani sp. nov. from Taiwan, with a key to the species of Taiwanese Lucanus Scopoli (Coleoptera: Lucanidae) . Jpn J Syst Entomol . 24 :257 –263 . Yang W , Zhang Y , Feng S , Liu L , Li Z 2018 The first complete mitochondrial genome of the Japanese beetle Popillia japonica (Coleoptera: Scarabaeidae) and its phylogenetic implications for thesuperfamily Scarabaeoidea . Int J Biol Macromol . 118 :1406 –1413 .29959994