==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1669081 1669081 Version of RecordResearch Article Mitogenome Announcement The near-complete mitogenome of the critically endangered Pseudocleopatra dartevellei (Caenogastropoda: Paludomidae) from the Congo River assembled from historical museum material B. Stelbrink et al.https://orcid.org/0000-0002-7471-4992Stelbrink Björn a https://orcid.org/0000-0001-9622-0566Kehlmaier Christian b https://orcid.org/0000-0001-8263-7758Wilke Thomas a https://orcid.org/0000-0002-1490-1825Albrecht Christian a a Department of Animal Ecology and Systematics, Justus Liebig University Giessen, Giessen, Germany; b Museum of Zoology, Senckenberg Dresden, Dresden, Germany CONTACT Björn Stelbrink bjoern.stelbrink@allzool.bio.uni-giessen.deDepartment of Animal Ecology and Systematics, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32 (IFZ), 35392 Giessen, Germany 25 9 2019 2019 4 2 3229 3231 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Here, we present the first near-complete mitogenome of a member of the freshwater gastropod family Paludomidae, Pseudocleopatra dartevellei. This Congo River species is of particular importance because the sister to the Lake Tanganyika radiation is supposed to be a paludomid riverine species. We used ancient DNA (aDNA) techniques including single-stranded DNA library preparation in order to assemble the mitogenome from historical museum material collected in 1937. The mitogenome was 15,368 bp long and showed typical characteristics as identified in other freshwater gastropods. The present phylogeny shows a closer relationship between Pseudocleoptra dartevellei and another non-Tanganyikan species, Cleopatra johnstoni. Keywords AfricaDemocratic Republic of the Congofreshwater gastropodsancient DNAmuseum genomics ==== Body Lake Tanganyika is home to a famous radiation of freshwater gastropods of the family Paludomidae (17 genera; Brown 1994; Neiber and Glaubrecht 2019). There is an ongoing discussion on whether Lake Tanganyika may have acted as a refuge for ancient lineages that existed in pre-lake systems such as the Congo River (Wilson et al. 2004). However, studies aiming at uncovering the biogeographical origin of the species flock have been hampered by the inaccessibility of key areas in the Congo Basin due to civil wars and unrest (Glaubrecht and Strong 2007). Under such circumstances, material conserved in institutional collections such as museums proves very valuable. This is also the case for the critically endangered riverine genus Pseudocleopatra (see Jørgensen 2010), which occurs in western Africa and parts of the Congo basin and which represents one of the potential sister groups of the Lake Tanganyika gastropod radiation. A specimen of Pseudocleopatra dartevellei Mandahl-Barth, 1973 was collected by Edmond Dartevelle in the Congo River near Matadi, Democratic Republic of the Congo (formerly Belgian Congo; 5.8167°S 13.4667°E) in 1937 and is deposited in the Royal Museum of Central Africa, Tervuren, Belgium (RMCA 233464). The material was analyzed applying NGS protocols developed for ancient and heavily degraded DNA in the cleanroom facility of the Senckenberg Natural History Collections, Dresden. Genomic DNA was extracted from c. 3 mm3 of soft tissue using the GEN-IAL All-tissue DNA-Kit (GEN-IAL GmbH, Troisdorf, Germany) protocol for forensic material and converted into a single-indexed single-stranded Illumina sequencing library (Gansauge and Meyer 2013; Korlević et al. 2015), including an uracil-DNA glycosylase (UDG) treatment. Shotgun sequencing was conducted on an Illumina MiSeq® platform (75 bp paired-end reads). After adapter trimming, quality filtering, and duplicate removal, reads mapping to a set of non-mollusk mitogenomes were excluded using FastQ Screen 0.11.4 (Wingett et al. 2018). The reduced readpool (c. 13.4 million reads) was then mapped against Cerithidea obtusa (NC_039951) using BWA 0.7.15 (Li and Durbin 2009). The obtained fragmentary consensus was used as a seed reference for a two-step baiting and iterative mapping approach in MITObim 1.9 (Hahn et al. 2013), with an allowed mismatch value of 2, resulting in a single high-quality contig (assembled reads: 24,308; average read length: 51 bp; maximum coverage = 211; average coverage = 82). The contig was visualized and checked for coverage and assembly artifacts in Tablet 1.16.09.06 (Milne et al. 2013), and the authenticity of the mapped reads was tested with mapDamage 2.0 (Jónsson et al. 2013). The contig was finally annotated on the MITOS2 Web Server (Bernt et al. 2013; reference dataset = RefSeq 63 Metazoa). The almost complete, annotated mitogenome has 15,368 bp (with only three ambiguous sites) and is deposited at GenBank (MN082637). The base composition was biased towards a high A + T content (63.72%; A = 28.16%, T = 35.56%), whereas the G + C content was comparatively low (36.26%; G = 19.44%, C = 16.82%). The majority of protein-coding genes (PCGs) started with an ATG (i.e. AUG) codon and stopped with a TAA codon. Gene arrangement on the light strand was: tRNACys, tRNAArg, tRNAAla, tRNAAsn, tRNATrp, tRNAGlu, tRNATyr, tRNALys, COX3, tRNAMet, COB, ND6, tRNAPro, ND1, tRNALeu2, tRNALeu1, 16S-rRNA, tRNAVal, tRNAGly, tRNAThr, and 12S-rRNA. On the heavy strand, gene order was: tRNASer1, ND2, tRNAAsp, ATP8, ATP6, tRNAIle, ND3, COX1, COX2, tRNASer2, tRNAGln, ND4L, ND4, tRNAHis, ND5, and tRNAPhe. Overlaps were identified between COB and ND6 (47 bp), tRNAThr and 12S-rRNA (3 bp), ND2 and tRNAAsp (2 bp), and ND5 and tRNAPhe (1 bp). For a phylogenetic reconstruction of the Paludomidae, available mitochondrial DNA sequences were downloaded from GenBank that refers to the study of Wilson et al. (2004). A maximum-likelihood tree based on partial sequences of COX1 and 16S-rRNA was constructed (Figure 1) using RAXML-HPC BLACKBOX 8.2.10 (Stamatakis 2014; settings: GTR + Γ model, codon partition scheme) on the CIPRES Science Gateway (Miller et al. 2010). Accordingly, Pseudocleopatra dartevellei is sister to Cleopatra johnstoni from Lake Mweru, a lake in the southeastern corner of the Congo drainage system. Although intergeneric relationships are often poorly supported, the present phylogeny supports a previous study that suggested a non-monophyly of the Lake Tanganyika paludomids (see Wilson et al. 2004). Figure 1. COX1–16S-rRNA maximum-likelihood phylogeny based on GenBank data (Wilson et al. 2004). The non-Tanganyikan species Pseudocleopatra dartevellei (Congo River) and Cleopatra johnstoni (Lake Mweru, Zambia) are marked in bold; the two Melanoides species were used as outgroups. GenBank accession numbers are shown in grey (left: COX1, right: 16S-rRNA). Acknowledgements We are especially grateful to Christophe Allard (Royal Museum for Central Africa, Tervuren, Belgium) for providing material of Pseudocleopatra dartevellei and other African species. Laboratory work was conducted at the cleanroom facility of the Museum of Zoology, Senckenberg Dresden (SGN-SNSD-Mol-Lab). Disclosure statement The authors report no conflict of interest. ==== Refs References Bernt M , Donath A , Jühling F , Externbrink F , Florentz C , Fritzsch G , Pütz J , Middendorf M , Stadler PF 2013 MITOS: improved de novo metazoan mitochondrial genome annotation . Mol Phylogenet Evol . 69 :313 –319 .22982435 Brown DS 1994 Freshwater snails of Africa and their medical importance . London (UK) : Taylor and Francis . Gansauge MT , Meyer M 2013 Single-stranded DNA library preparation for the sequencing of ancient or damaged DNA . Nat Protoc . 8 :737 –748 .23493070 Glaubrecht M , Strong EE 2007 Ancestry to an endemic radiation in Lake Tanganyika? 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