==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1676173 1676173 Version of RecordResearch Article Mitogenome Announcement The complete mitochondrial genome of Pareuchiloglanis myzostoma (Teleostei, Siluriformes) L. Cui et al.Cui Lili ab Gao Haitao ab Miao Xiangjun b Li Mingli a Li Guanghua b Xu Gefeng c Wu Junjie b Hu Wei a Lu Shaoxiong a a College of Animal Science and Technology, Yunnan Agricultural University, Kunming, PR China; b Yunnan Academy of Fishery Sciences, Kunming, PR China; c Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, PR China CONTACT Shaoxiong Lu shxlu@263.netCollege of Animal Science and Technology, Yunnan Agricultural University, Kunming650000, PR China 18 10 2019 2019 4 2 3626 3627 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Pareuchiloglanis myzostoma is a key-listed protected indigenous fish species in Nujiang, Yunnan, China. In this study, we firstly reported the complete mitochondrial genome of P. myzostoma, which was 16,584 bp in length, containing 13 protein-coding genes, 22 transfer RNAs (tRNAs), 2 ribosomal RNA genes (rRNAs), and a non-coding control region (D-loop). The overall base composition of P. myzostoma was 30.7% for A, 24.2% for T, 16.0% for G, and 29.1% for C. Phylogenetic analysis showed that all Sisoridae species clustered together formed a monophyletic group. This work would provide a set of useful data on further molecular evolution studies of this precious species. Keywords Pareuchiloglanis myzostomamitochondrial genomephylogeny ==== Body Pareuchiloglanis myzostoma (Norman 1923), commonly known as flat-headed fish, which belong to order Siluriformes, family Sisoridae, and genus Pareuchiloglanis, is a unique and rare fish in Nujiang, Yunnan Province (Zhou et al. 2005). It has been listed in the Chinese Vertebrate Red List (Jiang et al. 2016) and the Nujiang Key Conservation List of Indigenous Fish (http://yunnan.mofcom.gov.cn). In this study, we determined the complete mtDNA sequence of P. myzostoma. Samples were collected from Nujiang county of Yunnan Province in China (26°53′N; 99°07′E). The specimen is stored in the Specimen Museum of Yunnan Academy of Fishery Sciences and its accession number is 20180903001. The sequencing results were assembled using NOVOPlasty (https://github.com/ndierckx/NOVOPlasty). Genomes were predicted using the MitoAnnotator (http://mitofish.aori.u-tokyo.ac.jp/annotation/input.html). The transfer RNA (tRNA) genes were identified using the programme tRNAscan-SE (Lowe and Eddy 1997). The locations of protein-coding genes were determined by comparing with the corresponding known sequences of other Pareuchiloglanis fish species. The whole mitochondrial genome length of P. myzostoma was 16,584 bp in length (GenBank accession number MK617319). It consisted of a non-coding control region (D-loop), 13 protein-coding genes, 2 ribosomal RNA genes (rRNAs), and 22 tRNAs. The contents of A, C, G, and T were 30.7%, 29.1%, 16.0%, and 24.2%. The percentage of G + C content was 45.1%, which was lower than that of A + T content (54.9%). To demonstrate the phylogenetic position of P. myzostoma, we performed MEGA version 7.0 (Arizona State University, Phoenix, State of Arizona, United States of America) (Kumar et al. 2016) to align all selected sequences and construct a neighbour-joining tree containing complete mitochondrial genome DNA of 23 species. The results from the phylogenetic analysis revealed that all Sisoridae species clustered together formed a monophyletic group and P. myzostoma has a close relationship with Pseudexostoma yunnanensis. However, the genus Pareuchiloglanis was not a monophyletic group because it was also clustered with other genus Bagarius, Pseudexostoma, Creteuchiloglanis, and Euchiloglanis (Figure 1). Figure 1. A neighbour-joining (NJ) tree of the 23 species from Siluriformes was constructed based on complete mitochondrial genome data. The analysed species and corresponding NCBI accession numbers are as follows: Bagarius yarrelli (KP342264.1), Pareuchiloglanis gracilicaudata (JQ026237.1), Pseudexostoma yunnanensis (JQ026258.1), Creteuchiloglanis gongshanensis (KP872697.1), P. myzostoma (MK617319), Pareuchiloglanis sinensis (KJ637323.1), Pareuchiloglanis longicauda (KP872693.1), Pareuchiloglanis macrotrema (KP872694.1), Oreoglanis jingdongensis (KP872691.1), Oreoglanis macropterus (JQ026261.1), Euchiloglanis kishinouyei (JQ026252.1), Pareuchiloglanis anteanalis (KP872692.1), Creteuchiloglanis kamengensis (JQ026253.1), Glyptosternon maculatum (JQ026251.1), Hara jerdoni (AP012012.1), Glyptothorax lanceatus (NC_039895.1), Glyptothorax laosensis (NC_039702.1), Hemibagrus guttatus (KJ584373.1), Mystus cavasius (KU870465.1), Horabagrus brachysoma (KU870467.1), Horabagrus nigricollaris (MG986722.1), Cyprinus carpio (NC_001606.1), and Rhodeus ocellatus kurumeus (AB070205.1). Disclosure statement The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. ==== Refs References Jiang ZG , Jiang JP , Wang YZ , Zhang E , Zhang Y , Xie F , Li L , Cai B , Cao L , Zheng G , et al. 2016 Chinese Vertebrate Red List . Biodiver Sci . 5 :501 –551 . Kumar S , Stecher G , Tamura K 2016 MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets . Mol Biol Evol . 33 :1870 –1874 .27004904 Lowe TM , Eddy SR 1997 tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence . Nucleic Acids Res . 25 :955 –964 .9023104 Norman JR 1923 LIX.-Three new fishes from Yunnan, collected by Professor JW Gregory, FRS . Ann Mag Nat Hist . 11 :561 –563 . Zhou W , Li X , Yang Y 2005 Progress of studies on the phylogeny and geographic distribution pattern of catfish glyptosternoid (Sisoridae) in China . Zool Res . 26 :673 –679 .