==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1674209 1674209 Version of RecordResearch Article Mitogenome Announcement The complete chloroplast genome sequence of Oberonia seidenfadenii (Orchidaceae), a rare plant species endemic to China M. Jiang et al.Jiang Ming a Wang Junfeng b Chen Minghui a a Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation, College of Life Science, Taizhou University, Jiaojiang, Zhejiang, China; b Lishui Institute of Forestry, Lishui, Zhejiang, China CONTACT Ming Jiang jiangming1973@139.comZhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation, College of Life Science, Taizhou University, Jiaojiang318000, Zhejiang, China 4 10 2019 2019 4 2 3362 3363 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Oberonia seidenfadenii is a rare and newly recorded plant species in Zhejiang province, China. In our present study, the complete chloroplast (cp) genome sequence of O. seidenfadenii was assembled by using high-throughput Illumina sequencing data. The plastome is 143,062 bp in size, which contains a typical quadripartite structure with a pair of inverted repeats (IR) regions (24,278 bp) separated by a small single-copy (SSC) region (10,224 bp) and a large single-copy (LSC) region (84,282 bp). The cp genome sequence contains 127 genes, including 74 protein-coding genes, 38 tRNA genes, 8 rRNA genes, and 7 pseudogenes. Phylogenetic analysis results indicated O. seidenfadenii is a sister of Oberonia japonica, with a support rate of 100%. Keywords Oberonia seidenfadeniichloroplast genomephylogenetic analysis ==== Body The genus Oberonia belongs to the family Orchidaceae and consists of 150–200 species, and the Oberonia plants are epiphytic or lithophytic (Wu et al. 2009). Oberonia is a taxonomically complex genus and some species only shows slight differences in the morphology of leaves or flowers (Li et al. 2016). There are about 33 species distributed in China, and 11 of them are characterized as endemics (Wu et al. 2009). Oberonia seidenfadenii is a tiny Orchidaceae plant species with distichous-equitant leaves, densely clustered inflorescences, and greenish flowers. Oberonia seidenfadenii is a species native to China, and it mainly distributes in Taiwan, Guangxi, Guangdong, and Zhejiang (Huang 2000; Tian et al. 2013). In Zhejiang province, its populations are extremely small, and they are found only in some counties of Taizhou and Ningbo with less than 1000 individual plants. The information on chloroplast (cp) genome sequences of Oberonia is very limited, and the complete cp genome of O. seidenfadenii has not been characterized. In our present study, we assembled the cp genome of O. seidenfadenii by using high-throughput sequencing data, and a phylogenetic tree was generated to reveal its relationship with other species. Leaf samples were collected at an altitude of 36 m on Toumen Island (28°41′35′′N, 121°46′14′′E), Linhai County, Taizhou, Zhejiang province, China. A voucher specimen (CHS2017009) is deposited at the Molecular Biology Laboratory in Taizhou University. Total genomic DNA was extracted by using the CTAB method (Doyle and Doyle 1987), and a DNA library was constructed. The library was then sequenced on the Illumina Hiseq X Ten platform (Illumina, San Diego, CA). A total of 5.5 Gb raw 150 bp paired-end reads were generated, and the filtered reads were de novo assembled by the programme NOVOPlasty (Dierckxsens et al. 2017). The cp genome was annotated by Dual Organellar GenoMe Annotator (DOGMA), tRNAscan-SE, and ARAGORN (Lohse et al. 2004; Laslett and Canback 2004; Wyman et al. 2004; Lowe and Eddy 1997). The plastome of O. seidenfadenii (GenBank accession: MN414241) is 143,062 bp in size with an overall GC content of 37.1%. The cp genome consists of two inverted repeat (IR) regions, a large single-copy (LSC) region, and a small single-copy (SSC) region, and the sizes of IR, SSC, and LSC were 24,278, 10,224, and 84,282 bp, respectively. The GC contents of O. seidenfadenii IR, LSC, and SSC are 43.7, 34.4, and 27.9%, respectively. The genome encodes 127 genes, including 74 protein-coding genes, 38 tRNA genes, 8 rRNA genes, and 7 pseudogenes. Among these genes, four rRNAs (rrn4.5, rrn5, rrn16, and rrn23), eight tRNAs (trnA-UGC, trnH-GUG, trnI-CAU, trnI-GAU, trnL-CAA, trnN-GUU, trnR-ACG, and trnV-GAC), nine protein-coding genes (ndhB, rpl2, rpl22, rpl23, rps7, rps12, rps19, ycf1, and ycf2) contain two copies. One copy each of ndhJ, ycf1, ndhD, ndhF, rpl22, and two copies of ndhB genes were identified as pseudogenes. To understand the phylogenetic relationship with other Orchidaceae species, whole-genome sequences of 26 plants were obtained from NCBI, these included five Dendrobium species (Dendrobium officinale, Dendrobium hercoglossum, Dendrobium chrysotoxum, Dendrobium aphyllum, and Dendrobium aduncum), three Holcoglossum species (Holcoglossum weixiense, Holcoglossum nagalandense, and Holcoglossum amesianum), two Neofinetia species (Neofinetia falcata and Neofinetia richardsiana), as well as other 15 species from genera of Phalaenopsis, Vanda, Pelatantheria, Masdevallia, Gastrochilus, Epipactis, Oberonia, and Cephalanthera. Burmannia disticha (Burmanniaceae) was used as an outgroup. A phylogenetic tree was constructed by the maximum-likelihood method using PhyML 3.1 (Guindon et al. 2010). The results revealed that O. seidenfadenii grouped with Oberonia japonica, a morphologically similar plant species, exhibiting bootstrap support of 100% (Figure 1). Figure 1. A maximum-likelihood tree based on the complete chloroplast (cp) genome sequences of Oberonia seidenfadenii and other 25 Orchidaceae species, with Burmannia disticha as the outgroup. The numbers next to nodes are bootstrap support values. Disclosure statement No potential conflict of interest was reported by the authors. ==== Refs References Dierckxsens N , Mardulyn P , Smits G 2017 NOVOPlasty: de novo assembly of organelle genomes from whole genome data . Nucleic Acids Res . 45 :e18 .28204566 Doyle JJ , Doyle JL 1987 A rapid DNA isolation procedure for small quantities of fresh leaf tissue . Phytochem Bull . 19 :11 –15 . Guindon S , Dufayard JF , Lefort V , Anisimova M , Hordijk W , Gascuel O 2010 New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0 . Syst Biol . 59 :307 –321 .20525638 Huang TC 2000 Flora of Taiwan . Vol. 5. Taipei (Taiwan) : Sandos Chromagraph Printing Company . 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