==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1674741 1674741 Version of RecordResearch Article Mitogenome Announcement Mitochondrial DNA sequence of the hybrid of Pelteobagrus vachelli (♀) × Pelteobagrus fulvidraco (♂) T. Pan et al.Pan Tingshuang ab Ling Jun ab Duan Guoqing ab Jiang He ab a Fisheries Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China; b Anhui Province Key Laboratory of Aquaculture & Stock Enhancement, Hefei, Anhui, China CONTACT He Jiang 249725664@qq.comFisheries Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui, China 9 10 2019 2019 4 2 3398 3399 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract The complete mitochondrial genome sequence of a hybrid, which was produced by sexual hybridization of Pelteobagrus vachelli (♀) × Pelteobagrus fulvidraco (♂), was obtained. The complete mitochondrial genome of the hybrid is 16,532 bp, containing 37 genes. 129 Site differences were found in overall length compared with P. vachelli. Keywords Pelteobagrus vachellihybridPelteobagrus fulvidraco ==== Body Pelteobagrus vachelli and Pelteobagrus fulvidraco both belong to Bagridae. In this study, a hybrid was produced by female P. vachelli and male P. fulvidraco. Moreover, the complete mitochondrial genome of the hybrid was sequenced and compared with P. vachelli and constructed a phylogenetic tree by 13 protein-coding genes. The hybrid individuals were collected from Fisheries Institute, Anhui Academy of Agricultural Sciences, Hefei, China (31°53′33″N, 117°15′9″E). Voucher specimen (HB20190530-6) was deposited in Fish Specimen Library at Fisheries Institute Anhui Academy of Agriculture Sciences, Anhui, China. We amplified mitochondrial DNA using PCR method as reported (Pan et al. 2019). The complete mitochondrial genome of the hybrid is 16,532 bp (MN365880), containing a total of 37 genes. The 13 protein-coding genes in the mitochondrial genome of the hybrid also share features in start and stop codons with those in other fishes in Bagridae (Liang et al. 2013; Liang et al. 2016). Compared with the mitochondrial sequence of P. vachelli (MK134689), 129 Site differences were found between their mitochondrial genome. About 8 site differences were located in rRNAs, 1 in tRNAs, 9 in control region, and 111 in protein coding genes. Furthermore, 111varial bases of the protein coding regions resulted in 18 variable amino acids, which were located in ND1, COX2, ATP6, COX3, ND4, and ND5. Phylogenetic analysis was performed using 13 protein-coding genes by neighbor-joining (NJ). The results showed that the hybrid of P. vachelli (♀) × P. fulvidraco (♂) were genetically closer with P. vachelli than P. fulvidraco (Figure 1). Figure 1. Neighbor-joining phylogenetic tree inferred from amino acid sequence dataset of 13 protein-coding genes. The tree shows the topology based on concatenated data of 13 mitochondrial encoded protein sequences. Reconstruction was performed by MEGA X (64-bit). Acknowledgements We thank Dr. Maocang Yan for his comments on the manuscript. Disclosure statement The authors report no conflict of interest. The authors alone are responsible for the content and writing of the paper. ==== Refs References Liang HW , Li Z , Zou GW 2013 Complete mitochondrial DNA genome of Leiocassis crassilabris (Siluriformes: Bagridae) . Mitochondrial DNA . 24 :222 –224 .23311344 Liang HW , Li Z , Zou GW , Shi MY 2016 Complete mitochondrial DNA genome of Pseudobagrus medianalis (Siluriformes: Bagridae) . Mitochondrial DNA Part A . 27 :587 –588 . Pan T , Ling J , Jiang H , Duan G , Hu Y , Zhou H , Wang H , Cui K 2019 The complete mitochondrial genome of Pelteobagrus vachellii . Mitochondrial DNA Part B . 4 :828 –829 .