==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1667913 1667913 Version of RecordResearch Article Mitogenome Announcement Mitochondrial genome of Diaphorencyrtus aligarhensis (Hymenoptera: Chalcidoidea: Encyrtidae) and phylogenetic analysis Y. Du et al.Du Yimin ab Song Xiang ab Liu Xinjun ab Zhong Balian ab a School of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi, China; b National Navel Orange Engineering and Technology Research Center, Ganzhou, Jiangxi, China CONTACT Balian Zhong zhongbl008@163.comSchool of Life Sciences, Gannan Normal University, Ganzhou341000, Jiangxi, China 23 9 2019 2019 4 2 3190 3191 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Diaphorencyrtus aligarhensis is an important natural enemy of the psyllid Diaphorina citri Kuwayama, a vector of the huanglongbing (HLB). Here, we sequenced and annotated the mitochondrial genome (mitogenome) of D. aligarhensis. This mitogenome was 16,264 bp long and encoded 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), and two ribosomal RNA unit genes (rRNAs). All 13 PCGs were initiated by the ATN (ATG, ATT, ATA, and ATC) codon. All PCGs terminate with the stop codon TAA except for cox2 and nad1 which end with the incomplete codon T−. Phylogenetic analysis showed that D. aligarhensis got together with the same family species Encyrtus infelix, and Encyrtidae had a close relationship with Agaonidae. Keywords Encyrtidaemitochondrial genomeDiaphorencyrtus aligarhensisphylogenetic analysis ==== Body Diaphorencyrtus aligarhensis (Shafee, Alam, and Argarwal) is an endoparasitoid of Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae). Diaphorencyrtus aligarhensis was originally recorded in India and was imported into many countries as biological control agents for D. citri (Shafee et al. 1975; Rohrig et al. 2011). Specimens of D. aligarhensis were collected from Puning City, Guangdong Province, China (23°21′N, 116°2′E, October 2018) and were stored in Entomological Museum of Gannan Normal University (Accession number GNU-DA017). After morphological identification, total genomic DNA was extracted from tissues using DNeasy DNA Extraction kit (Qiagen, Hilden, Germany). The mitogenome sequence of D. aligarhensis was generated using Illumina HiSeq 2500 Sequencing System (Illumina, San Diego, CA). In total, 6.9 G raw reads were obtained, quality-trimmed, and assembled using MITObim v 1.7 (Hahn et al. 2013). By comparison with the homologous sequences of other Chalcidoidea species from GenBank, the mitogenome of D. aligarhensis was annotated using software GENEIOUS R8 (Biomatters Ltd., Auckland, New Zealand). The nearly complete mitogenome of D. aligarhensis is 16,264 bp (Genbank accession, MN274569). It contains 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes, and a partial non-coding AT-rich region. The putative control region located between trnM and nad2, which was failed to sequence and was similar to other Chalcidoidea species (Su et al. 2016; Zhu et al. 2018; Tang et al. 2019). The nucleotide composition of the mitogenome was biased toward A and T, with 81.8% of A + T content (A 46.8%, T 35.0%, C 11.5%, G 6.7%). Mitogenome of D. aligarhensis exhibit dramatic mitochondrial gene rearrangement which is common in Chalcidoidea species (Chen et al. 2018; Xiong et al. 2019). All 13 PCGs of D. aligarhensis have the conventional ATN start codons for invertebrate mitochondrial PCGs (5 ATG, 5 ATT, 2 ATA, and 1 ATC). Most of the PCGs terminate with the stop codon TAA, whereas cox2 and nad1 end with the incomplete codon T. The 22 tRNA genes vary from 56 bp (trnE) to 77 bp (trnC). Two rRNA genes (rrnL and rrnS) are located at trnL1/trnA and trnA/trnV regions, respectively. The lengths of rrnL and rrnS in D. aligarhensis are 1310 and 798 bp, with the same AT contents of 86.6%. All 13 mitochondrial protein-coding genes sequences were extracted from the complete or nearly complete mitochondrial DNA sequences of 17 closely related taxa of Chalcidoidea, including two outgroup species from Cynipoidea and Proctotrupoidea. The phylogenetic tree was constructed using the maximum-likelihood method through raxmlGUI 1.5 (Silvestro and Michalak 2012). The newly sequenced species D. aligarhensis got together with the same family species Encyrtus infelix with high support value (BS = 100), and Encyrtidae had a close relationship with Agaonidae (Figure 1). In conclusion, the mitogenome of D. aligarhensis is sequenced in this study and can provide important DNA molecular data for further phylogenetic and evolutionary analysis of Chalcidoidea. Disclosure statement The authors report no conflict of interest. The authors alone are responsible for the content and writing of the article. Figure 1. Phylogenetic relationships based on the 13 mitochondrial protein-coding genes sequences inferred from RaxML. 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