==== Front Mitochondrial DNA B Resour Mitochondrial DNA B Resour Mitochondrial DNA. Part B, Resources 2380-2359 Taylor & Francis 10.1080/23802359.2019.1640647 1640647 Version of RecordResearch Article Mitogenome Announcement Analysis of the complete organellar genomes of the economically valuable kelp Lessonia spicata (Lessoniaceae, Phaeophyceae) from Chile D. Tineo et al.Tineo Daniel a* Rubio Karol B. a* Melendez Jegnes B. a* Mendoza Jani E. a* Silva Jhonsy O. a* Perez Jhordy a* Esquerre Eggleantina E. a* Perez-Alania Melissa b* Fernandez Samia L. a* Aguilar Smith E. c* Chuquizuta Fernando c* Olano Yadira M. c* Hoyos Renzo P. c* Veneros Jaris E. d* Garcia Ligia M. e* Arakaki Natalia f* Garcia-Candela Enrique g* Oliva Manuel a* Mansilla Andres g* https://orcid.org/0000-0003-3611-140XCalderon Martha S. ah* https://orcid.org/0000-0003-4053-9150Hughey Jeffery R. i* https://orcid.org/0000-0002-5979-6993Bustamante Danilo E. a* a Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza, Amazonas, Peru; b Facultad de Ciencias, Universidad Nacional Agraria La Molina, Lima, Peru; c Facultad Ingeniería Zootecnista, Agronegocios y Biotecnología (FIZAB), Universidad Nacional Toribio Rodríguez de Mendoza, Amazonas, Peru; d Facultad de Ingeniería Civil y Ambiental (FICIAM), Universidad Nacional Toribio Rodríguez de Mendoza, Amazonas, Peru; e Facultad de Ingeniería y Ciencias Agrarias (FICA), Universidad Nacional Toribio Rodríguez de Mendoza, Amazonas, Peru; f Banco de Germoplasma de Organismos Acuáticos, Instituto del Mar del Perú, Callao, Peru; g Instituto Tecnológico de la Producción, CITEacuícola Ahuashiyacu, San Martin, Peru; h Laboratorio de Ecosistemas Marinos Antárticos y Sub-antárticos (LEMAS), Universidad de Magallanes, Punta Arenas, Chile; i Division of Mathematics, Science, and Engineering, Hartnell College, Salinas, CA, USA * All authors contributed equally to the analysis and writing of this paper. CONTACT Danilo E. Bustamante ddanilobm@gmail.comInstituto de Investigación para el Desarrollo Sustentable de Ceja de Selva (INDES-CES), Universidad Nacional Toribio Rodríguez de Mendoza, Amazonas01001, Peru 16 7 2019 2019 4 2 2581 2582 © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.2019The Author(s)This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/Abstract Lessonia spicata (Suhr) Santelices is the most ecologically and economically important kelp from Pacific South America. Here, we contribute to the bioinformatics and evolutionary systematics of the species by performing high throughput sequencing on L. spicata from Valparaiso, Chile. The L. spicata complete mitogenome is 37,097 base pairs (bp) in length and contains 66 genes (GenBank accession MK965907), the complete plastid genome is 130,305 bp and has 173 genes (accession MK965908), and the data assembled 7,630 bp of the nuclear ribosomal cistron (accession MK965909). The organellar genomes are similar in structure and content to others published from the Laminariales. Keywords ChilekelpLessoniamitogenomeplastid genome ==== Body Lessonia spicata is a common intertidal to shallow subtidal kelp distributed from central (29° S) to southern (41° S) Chile (González et al. 2012). This species is characterized as having a dichotomous stipe with longitudinal splits and numerous branches of the thallus, with each branch with a single, narrow, terminal blade (von Suhr 1839; Searles 1978). Lessonia spicata is considered the most ecologically important and dominant seaweed on the Pacific South American coast (Santelices et al. 1980; González et al. 2012). It is also economically valuable and is harvested for the phycocolloid alginate (Díaz et al. 2012). To contribute to the evolutionary systematics of the Laminariales and to advance the understanding of the taxonomy of L. spicata, this study characterized the complete organellar genomes and the nuclear ribosomal cistron of L. spicata from Reñaca beach, Valparaiso, Chile. DNA was extracted from L. spicata (Specimen Voucher-DBM0003) using the Quick-DNA Plant/Seed kit (Zymo Research, California, USA) following the manufacturer’s instructions. The 150 bp PE Illumina library construction and sequencing was performed using myGenomics, LLC (Alpharetta, Georgia, USA). The genomes were assembled using default de novo settings in CLC Genomics Workbench 12.0 (QIAGEN Bioinformatics, Redwood City, CA, USA) and Geneious Prime to close gaps (Biomatters, Ltd, Auckland, New Zealand). The genes were annotated manually using blastx, NCBI ORFfinder, and tRNAscan-SE 1.21 (Schattner et al. 2005). The L. spicata mitogenome was aligned to other mitogenomes using MAFFT (Katoh and Standley 2013). The phylogenetic analysis was executed with RAxML-NG (Kozlov et al. 2018) using the GTR + gamma model and 1000 bootstraps. The tree was visualized with TreeDyn 198.3 at Phylogeny.fr (Dereeper et al. 2008). The mitogenome of L. spicata is 37,097 bp in length and contains 66 genes. It is A + T rich (67.3%) and includes 25 tRNA (trnK and trnS occur in duplicate, trnL and trnM in triplicate), 17 ribosomal proteins, three rRNA (rnl, rns, rrn5), three orfs (orf41, orf129, orf378), and 18 other genes involved in electron transport and oxidative phosphorylation. The plastid genome of L. spicata is 130,305 bp and contains 173 genes. It is A + T biased (69.1%) and includes 45 ribosomal proteins, 27 tRNA (trnA, trnG, trnI, trnR, and trnS occur in duplicate, trnM occurs in triplicate), 27 photosystem I and II, 20 ycf, eight cytochrome b/f complex, eight ATP synthase, four RNA polymerase, six rRNA, and 28 other genes. The mitogenome and plastid genome of L. spicata are similar in length, content, and organization to other Laminariales (Oudot-le secq et al. 2006; Yotsukura et al. 2010; Li et al. 2015; Qu et al. 2015; Zhang et al. 2015; Chen et al. 2019; Zheng et al. 2019). Phylogenetic analysis of the L. spicata mitogenome positions it in a clade with Laminaria digitata and L. hyperborea (Figure 1). This evolutionary relationship is similar to the most recent multigene and transcriptome analyses of the Laminariales in which the Lessoniaceae is closely allied with the Laminariaceae (Kawai 2014; Kawai et al. 2017; Starko et al. 2019). Figure 1. Maximum likelihood phylogram of Lessonia spicata (MK965907) and related Laminarialean mitogenomes. Numbers along branches are RaxML bootstrap supports based on 1000 replicates. The legend below represents the scale for nucleotide substitutions. Disclosure statement The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this article. ==== Refs References Chen J , Zang Y , Shang S , Tang X 2019 The complete mitochondrial genome of the brown alga Macrocystis integrifolia (Laminariales, Phaeophyceae) . Mitochondrial DNA B . 4 :635 –636 . Dereeper A , Guignon V , Blanc G , Audic S , Buffet S , Chevenet F , Dufayard JF , Guindon S , Lefort V , Lescot M , et al. 2008 Phylogeny.fr: robust phylogenetic analysis for the non-specialist . Nucleic Acids Res . 36 :W465 –W469 .18424797 Díaz O , Tapia Y , Muñoz O , Montoro R , Velez D , Almela C 2012 Total and inorganic arsenic concentrations in different species of economically important algae harvested from coastal zones of Chile . Food Chem Toxicol . 50 :744 –749 .22138359 González A , Beltrán J , Hiriart-Bertrand L , Flores V , de Reviers B , Correa JA , Santelices B 2012 Identification of cryptic species in the Lessonia nigrescens complex (Phaeophyceae, LAMINARIALES)(1)) . J Phycol . 48 :1153 –1165 .27011275 Katoh K , Standley DM 2013 MAFFT multiple sequence alignment software version 7: improvements in performance and usability . Mol Biol Evol . 30 :772 –780 .23329690 Kawai H 2014 Recent advances in the phylogeny and taxonomy of Laminariales, with special reference to the newly discovered basal member Aureophycus . Persp Phycol . 1 :27 –40 . Kawai H , Hanyuda T , Gao X , Terauchi M , Miyata M , Lindstrom SC , Klochkova NG , Miller KA 2017 Taxonomic revision of the Agaraceae with a description of Neoagarum gen. nov. and reinstatement of Thalassiophyllum . J Phycol . 53 :261 –270 .28078742 Kozlov AM , Darriba D , Flouri T , Morel B , Stamatakis A 2018 . RAxML-NG: A fast, scalable, and user-friendly tool for maximum likelihood phylogenetic inference. BioRxiv 447110. Li TY , Qu JQ , Feng YJ , Liu C , Chi S , Liu T 2015 Complete mitochondrial genome of Undaria pinnatifida (Alariaceae, Laminariales, Phaeophyceae) . Mitochondrial DNA . 26 :953 –954 .24409911 Oudot-Le Secq MP , Loiseaux-de Goer S , Stam WT , Olsen JL 2006 Complete mitochondrial genomes of the three brown algae (Heterokonta: Phaeophyceae) Dictyota dichotoma, Fucus vesiculosus and Desmarestia viridis . Curr Genet . 49 :47 –58 .16317568 Qu J-Q , Liu C , Wang X-M , Zhang Z-B , Chi S , Liu T 2015 Complete mitochondrial genome of Costaria costata shows conservative evolution in Laminariales . Mitochondrial DNA . 26 :919 –920 .24409887 Santelices B , Castilla JC , Cancino J , Schmiede P 1980 Comparative ecology of Lessonia nigrescens and Durvillaea antartica (Phaeophyta) in central Chile . Mar Biol . 59 :119 –132 . Schattner P , Brooks AN , Lowe TM 2005 The tRNAscan-SE, snoscan and snoGPS web servers for the detection of tRNAs and snoRNAs . Nucl Acids Res . 33 :686 –689 . Searles RB 1978 The genus Lessonia Bory (Phaeophyta, Laminariales) in southern Chile and Argentina . Br Phycol J . 13 :361 –381 . Starko S , Soto-Gomez M , Darby H , Demes KW , Kawai H , Yotsukura N , Lindstrom S , Keeling PJ , Graham SW , Martone PT 2019 A comprehensive kelp phylogeny sheds light on the evolution of an ecosystem . Mol Phylogenet Evol . 136 :138 –150 .30980936 von Suhr JN 1839 Beiträge zur Algenkunde . Flora . 22 :65 –75 . Yotsukura N , Shimizu T , Katayama T , Druehl LD 2010 Mitochondrial DNA sequence variation of four Saccharina species (Laminariales, Phaeophyceae) growing in Japan . J Appl Phycol . 22 :243 –251 . Zhang L , Wang X , Liu T , Wang H , Wang G , Chi S , Liu C 2015 Complete plastid genome of the brown Alga Costaria costata (Laminariales, Phaeophyceae) . PLoS One . 10 :e0140144 26444909 Zheng Z , Chen H , Wang H , Jiang W , Cao Q , Du N 2019 Characterization of the complete mitochondrial genome of bull kelp . Nereocystis Luetkeana Mitochondrial DNA B . 4 :630 –631 .