==== Front Onco Targets TherOnco Targets TherOncoTargets and TherapyOncoTargets and therapy1178-6930Dove Medical Press 10.2147/OTT.S168274ott-11-4651Original ResearchRelationship of PPARG, PPARGC1A, and PPARGC1B polymorphisms with susceptibility to hepatocellular carcinoma in an eastern Chinese Han population Zhang Sheng 1*Jiang Jiakai 1*Chen Zhan 2Wang Yafeng 3Tang Weifeng 4Chen Yu 567Liu Longgen 8 1 Department of General Surgery, Changzhou Third People’s Hospital, Changzhou, Jiangsu Province, China 2 Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian Province, China 3 Department of Cardiology, People’s Hospital of Xishuangbanna Dai Autonomous Prefecture, Jinghong, Yunnan Province, China 4 Department of Cardiothoracic Surgery, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China 5 Cancer Bio-immunotherapy Center, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian Province, China, 13859089836@139.com 6 Department of Medical Oncology, Fujian Cancer Hospital and Fujian Medical University Cancer Hospital, Fuzhou, Fujian Province, China, 13859089836@139.com 7 Fujian Provincial Key Laboratory of Translational Cancer Medicine, Fuzhou, Fujian Province, China, 13859089836@139.com 8 Department of Liver Disease, Changzhou Third People’s Hospital, Changzhou, Jiangsu Province, China, jsllg0519@163.comCorrespondence: Longgen Liu, Department of Liver Disease, Changzhou Third People’s Hospital, Changzhou, Jiangsu 213001, China, Email jsllg0519@163.comYu Chen, Department of Medical Oncology, Fujian Cancer Hospital, Fujian Medical University Cancer Hospital, Fuzhou 350000, China, Email 13859089836@139.com* These authors contributed equally to this work 2018 08 8 2018 11 4651 4660 © 2018 Zhang et al. This work is published and licensed by Dove Medical Press Limited2018The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.Background PPARG, PPARGC1A, and PPARGC1B polymorphisms may be implicated in the development of cancer. Participants and methods In this study, we selected PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A single-nucleotide polymorphisms to explore the relationship between these polymorphisms and hepatocellular carcinoma (HCC) risk. A total of 584 HCC patients and 923 controls were enrolled. Results We found that PPARG rs1801282 C>G polymorphism was correlated with a decreased susceptibility of HCC (CG vs CC, adjusted OR 0.47, 95% CI 0.27–0.82, P=0.007; CG/GG vs CC, adjusted OR 0.52, 95% CI 0.31–0.88, P=0.015). However, PPARG rs3856806 C>T polymorphism was a risk factor for HCC (TT vs CC, adjusted OR 2.33, 95% CI 1.25–4.36, P=0.008; TT vs CT/CC, adjusted OR 2.26, 95% CI 1.22–4.17, P=0.010). In a subgroup analysis by chronic hepatitis B virus (HBV)-infection status, age, sex, alcohol use, and smoking status, a significant association between PPARG rs1801282 C>G polymorphism and a decreased risk of HCC in male, ≥53 years, never-smoking, never-drinking, and nonchronic HBV-infection-status subgroups was found. However, we found PPARG rs3856806 C>T polymorphism increased the risk of HCC in never-smoking, never-drinking, and nonchronic HBV-infection-status subgroups. Haplotype-comparison analysis indicated that Crs1801282Trs3856806Crs2970847Grs7732671Grs17572019, Crs1801282Trs3856806Trs2970847Grs7732671Grs17572019, and Crs1801282Crs3856806Crs2970847Crs7732671Ars17572019 haplotypes increased the risk of HCC. PPARG Crs1801282Trs3856806 and Grs1801282Crs3856806 haplotypes also influenced the risk of HCC. Conclusion In conclusion, our findings suggest PPARG polymorphisms may influence the susceptibility of HCC. The PPARG, PPARGC1A, and PPARGC1B haplotypes might be associated with HCC risk. Keywords PPARGPPARGC1APPARGC1Bpolymorphismriskhepatitis B virushepatocellular carcinoma ==== Body Introduction In 2012, an estimated 782,500 new liver cancer (LC) patients and 745,500 related deaths occurred worldwide.1 China accounts for almost half the total number of LC cases and deaths annually. Hepatocellular carcinoma (HCC) is the most common subtype of LC. A large number of HCC cases are diagnosed annually, with a high mortality rate, which encourages people to explore the potential risk factors for HCC. Due to the chronic infection of hepatitis B virus (HBV), the incidence of HCC in parts of sub-Saharan Africa and Asia is much higher than other regions.2,3 However, other risk factors might also contribute to the etiology of HCC. Recently, many hereditary factors have been found to confer susceptibility to HCC. Peroxisome proliferator-activated receptors (PPARs), a cluster of important nuclear transcription factors, may be involved in the process of cellular differentiation and regulate carbohydrate/lipid metabolism and energy balance.4 There are three predominant subtypes in PPARs: PPARα, PPARβ, and PPARγ.5 PPARγ, also known as PPARG, is located on chromosome 3p25. PPARG interacts with RXR and forms a dipolymer to regulate its target genes, which are involved in adipocyte differentiation and insulin sensitization.6 It has been reported that PPARG possessed anti-inflammatory roles7,8 and can restrain the production of many inflammatory mediators, such as IL6, IL8, and TNFα.9 Several studies have found that obesity, metabolic syndrome, insulin resistance/insufficiency, type 2 diabetes mellitus (T2DM), and inflammation have a common molecular basis, in which PPARG can influence the process of these diseases and might alter the risk of cancer.10–12 Two coactivators of PPARG, PPARGC1A and PPARGC1B, are vital regulators of energy metabolism.13 In addition, Li et al reported that PPARGC1A might be a potential biomarker for lung cancer prognosis.14 Eichner et al found that miR378 was embedded within PPARGC1B, which encodes PPARGC1B, and miR378 expression correlated with progression of breast cancer in humans.15 Recently, a meta-analysis found that PPARG rs1801282 C>G single-nucleotide polymorphism (SNP) was associated with cancer risk in Asians;16 however, the studies included were limited.16 PPARG rs3856806 C>T polymorphism is believed to be related to inflammatory response17 and is associated with the development of ovarian carcinoma,18 follicular lymphoma,19 and colorectal cancer.20–22 Studies have reported that PPARGC1A rs2970847 C>T SNP increased the risk of T2DM.23,24 However, the association between this SNP and cancer risk is unknown. Martínez-Nava et al studied the association of PPARGC1B rs7732671 G>C and rs17572019 G>A SNPs with risk of breast cancer and found that the PPARGC1B rs7732671 C allele was a protective factor for breast cancer.25 In view of these previous studies, the potential role of PPARG, PPARGC1A, and PPARGC1B SNPs in determining HCC risk was unclear. Understanding the possible relationship might be beneficial for HCC prevention. Therefore, in this case–control study, we selected PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A SNPs to explore the relationship between these polymorphisms and HCC risk in an eastern Chinese Han population. Participants and methods Subjects As part of an ongoing study carried out in an eastern Chinese Han population, the first 584 incident HCC patients and 923 hospital-based controls were recruited in this study. Our case–control study was approved by Fujian Medical University Ethics Committee (Fuzhou, China). HCC cases were recruited from the Department of Hepatobiliary Surgery at Fuzong Clinical Medical College and Union Clinical Medical College of Fujian Medical University. All HCC patients were diagnosed by pathology. Major selection criteria of HCC patients were sporadic HCC cases, HCC patients without chemoradiotherapy, Chinese Han population, and living in eastern China. Corresponding exclusion criteria were HCC patients with autoimmune disease history, had received prior chemoradiotherapy, had other malignancy history, and without a pathological diagnosis. Meanwhile, a total of 923 participants who attended a physical examination in the hospitals mentioned were enrolled as controls. Additionally, criteria for control selection were healthy subjects without a history of malignancy, without autoimmune disease, without chronic liver disease, and eastern Chinese Han. HCC patients and controls matched well by age and sex. All subjects were recruited between January 2002 and December 2016 consecutively. Demographic variables and risk factors (eg, smoking, drinking, and chronic HBV-infection status) were collected by our colleagues. Written informed consent was signed by all subjects. Information is listed in Table 1. DNA extraction and genotyping Extraction of genomic DNA from EDTA anticoagulant blood samples was performed using a DNA-purification kit (Promega, Madison, WI, USA). Purity and concentration of the DNA samples obtained was assessed by spectrophotometry with the NanoDrop ND-1000 and 1.5% agarose gel electrophoresis. Genomic DNA was stored at −80°C. Genotyping of PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A SNPs was carried out with a genotyping assay (SNPscan; Genesky Biotechnologies, Shanghai, China) on a 3730XL (Thermo Fisher Scientific, Waltham, MA, USA). Data were observed using GeneMapper 4.1 software (Thermo Fisher Scientific). Sixty (4%) randomly selected samples were tested again by a different technologist. The results were not altered. Statistical analysis All statistical analyses were done with SAS 9.4 software (SAS Institute, Cary, NC, USA) using Student’s t-test, Fisher’s exact test, and χ2-test. Age was expressed as the mean ± SD. We used Student’s t-test to determine the differences in age distribution between HCC cases and controls, and χ2-test or Fisher’s exact test used to assess potential differences in age, sex, smoking status, alcohol use, chronic HBV-infection status, and genotypes. Deviation from Hardy–Weinberg equilibrium (HWE) was determined using an Internet-based calculator (http://ihg.gsf.de/cgi-bin/hw/hwa1.pl)26,27 to compare the obtained genotype frequencies in controls with the expected frequencies. Using different models of inheritance (allele, additive, homozygote, dominant, and recessive), associations between PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A SNPs and risk of HCC were determined by crude/adjusted ORs and CIs. SHEsis software (Bio-X, Shanghai, China), an online calculator, was used for construction of PPARG, PPARGC1A, and PPARGC1B haplotypes.28 P<0.05 (two-tailed) was used as the threshold for significance. In this study, Bonferroni correction was performed for multiple testing.29,30 Power and Sample Size Calculation software (http://biostat.mc.vanderbilt.edu/twiki/bin/view/Main/PowerSampleSize) was used to assess the statistical power of this study (α=0.05).31 Results Baseline characteristics HCC patients and cancer-free controls comprised 584 and 923 subjects, respectively. Mean ages were 53.17±11.76 (range 20–83) years in the HCC group and 53.72±9.97 (range 21–83) years in controls. Baseline characteristics of HCC patients and controls are given in Table 1. In addition, Table 1 shows that our study was well matched by sex and age. Corresponding SNP information for PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A polymorphisms is summarized in Table 2. The success rate of genotyping was >99% (Table 2). The minor-allele frequency in controls is listed in Table 2, and results were similar to the data for Chinese Han population. In controls, except for PPARG rs3856806 C>T, the distribution of PPARG rs1801282 C>G, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A genotype frequencies accorded with HWE. Association of PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A SNPs with HCC The frequencies of PPARG rs1801282 genotypes in HCC patients and controls are summarized in Table 3. We found that the PPARG rs1801282 G allele was associated with a decreased risk of HCC (CG vs CC, crude OR 0.47, 95% CI 0.31–0.72, P=0.001; CG/GG vs CC, crude OR 0.51, 95% CI 0.34–0.77, P=0.001; G vs C, crude OR 0.56, 95% CI 0.38–0.82, P=0.003). After adjustments for age, sex, smoking, drinking, and chronic HBV-infection status, the results were not essentially changed (CG vs CC, adjusted OR 0.47, 95% CI 0.27–0.82, P=0.007; CG/GG vs CC, adjusted OR 0.52, 95% CI 0.31–0.88, P=0.015; Table 3). Table 3 lists the frequencies of PPARG rs3856806 genotypes in HCC patients and controls. We found that the PPARG rs3856806 T allele conferred risk to HCC (TT vs CC, crude OR 2.12, 95% CI 1.31–3.44, P=0.002; TT vs CC/CT, crude OR 2.13, 95% CI 1.33–3.43, P=0.002; T vs C, crude OR 1.21, 95% CI 1.02–1.44, P=0.029). After adjustments for age, sex, smoking, drinking, and chronic HBV-infection status, the results were not materially altered (TT vs CC, adjusted OR 2.33, 95% CI 1.25–4.36, P=0.008; TT vs CT/CC, adjusted OR 2.26, 95% CI 1.22–4.17, P=0.010; Table 3). However, PPARGC1A rs2970847 C>T and PPARGC1B rs7732671 G>C and rs17572019 G>A polymorphisms were not associated with HCC risk in all genetic models (Table 3). We performed a Bonferroni correction for multiple testing. The genotype distribution of PPARG polymorphisms was still significantly different between HCC cases and controls (P=0.007 for rs1801282 C>G, P=0.008 and P=0.010 for rs3856806 C>T, respectively). We also calculated the statistical power of this study (α=0.05) using Power and Sample Size Calculation.31 For PPARG rs1801282 C>G, the power value was 0.955 in CG vs CC, 0.906 in GG/CG vs CC, and 0.859 in G vs C. For PPARG rs3856806 C>T, the power value was 0.932 in TT vs CC, 0.921 in TT vs CC/CT, and 0.584 in T vs C. Association of PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A SNPs with HCC in different subgroups Table 4 shows the relationship of PPARG rs1801282 C>G polymorphism with risk of HCC in the stratified analyses. After adjustment by logistic regression analysis, we found that the PPARG rs1801282 G allele decreased the risk of HCC (male subgroup, CG vs CC, adjusted OR 0.52, 95% CI 0.29–0.92, P=0.025 and CG/GG vs CC, adjusted OR 0.52, 95% CI 0.29–0.91, P=0.022; ≥53 years subgroup, CG vs CC, adjusted OR 0.36, 95% CI 0.17–0.74, P=0.006 and CG/GG vs CC, adjusted OR 0.38, 95% CI 0.19–0.77, P=0.007; never-smoking subgroup, CG vs CC, adjusted OR 0.32, 95% CI 0.15–0.66, P=0.002 and CG/GG vs CC, adjusted OR 0.39, 95% CI 0.20–0.77, P=0.007; never-drinking subgroup, CG vs CC, adjusted OR 0.40, 95% CI 0.21–0.76, P=0.005 and CG/GG vs CC, adjusted OR 0.47, 95% CI 0.26–0.86, P=0.015; nonchronic HBV-infection subgroup, CG vs CC, adjusted OR 0.42, 95% CI 0.20–0.89, P=0.024 and CG/GG vs CC, adjusted OR 0.46, 95% CI 0.22–0.93, P=0.030). As listed in Table 5, we found that the PPARG rs3856806 T allele was associated with a risk of HCC in some subgroups (never-smoking subgroup, TT vs CC, adjusted OR 2.24, 95% CI 1.09–4.60, P=0.028 and TT vs CT/CC, adjusted OR 2.21, 95% CI 1.09–4.49, P=0.027; never-drinking subgroup, TT vs CC, adjusted OR 2.10, 95% CI 1.05–4.19, P=0.036 and TT vs CT/CC, adjusted OR 2.08, 95% CI 1.05–4.11, P=0.035; nonchronic HBV-infection subgroup, TT vs CC, adjusted OR 2.44, 95% CI 1.22–4.88, P=0.012 and TT vs CT/CC, adjusted OR 2.34, 95% CI 1.19–4.60, P=0.014). However, PPARGC1A rs2970847 C>T and PPARGC1B rs7732671 G>C and rs17572019 G>A polymorphisms were not associated with HCC risk in any subgroup (data not shown). SNP haplotypes Using the SHESIS online calculator,28 we constructed several haplotypes of PPARG, PPARGC1A, and PPARGC1B genes (Table 6). Haplotype comparison analysis indicated that Crs1801282Trs3856806Crs2970847Grs7732671Grs17572019, Crs1801282Trs3856806Trs2970847Grs7732671Grs17572019, and Crs1801282Crs3856806Crs2970847Crs7732671Ars17572019 were associated with risk of HCC (OR 1.29, 95% CI 1.05–1.59, P=0.017; OR 1.56, 95% CI 1.08–2.25, P=0.017; and OR 1.63, 95% CI 1.11–2.39, P=0.011, respectively). In addition, PPARG Crs1801282Trs3856806 and Grs1801282Crs3856806 haplotypes also influenced the risk of HCC (OR 1.31, 95% CI 1.09–1.57, P=0.004 and OR 0.46, 95% CI 0.21–1.00, P=0.046, respectively). Discussion PPARG, PPARGC1A, and PPARGC1B genes may have an impact on inflammatory response, insulin sensitization, cell differentiation, and cellular apoptosis32–35 and alter the risk of cancer. In this study, we examined the relationship between PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C and rs17572019 G>A SNPs and HCC risk. We found that PPARG rs1801282 C>G polymorphism decreased the risk of HCC. However, PPARG rs3856806 C>T was a risk factor for HCC. In subgroup analyses, we found that the PPARG rs1801282 C>G polymorphism decreased the risk of HCC in male, ≥53 years, never-smoking, never-drinking, and nonchronic HBV-infection-status subgroups. However, PPARG rs3856806 C>T polymorphism increased the risk of HCC in never-smoking, never-drinking, and nonchronic HBV-infection-status subgroups. Results of haplotype analysis suggested that CTCGG, CTTGG, and CCCCA hap-lotypes of the order PPARG rs1801282 C>G and rs3856806 C>T, PPARGC1A rs2970847 C>T, PPARGC1B rs7732671 G>C, and PPARGC1B rs17572019 G>A polymorphisms might confer risk of HCC. PPARG has been considered a HCC suppressor that contributes to the suppression of HCC-cell growth, angiogenesis, and migration.36–39 These primary results indicate that PPARG plays an important role in tumor suppression and may be a therapeutic target in HCC.36 An SNP can lead to abnormal expression or to the generation of a defective form of the protein. Therefore, SNPs may be associated with the development of disease. PPARG rs1801282 C>G polymorphism is a missense SNP, which encodes a proline-to-alanine substitution.40 Compared to the PPARG rs1801282 C allele, the PPARG rs1801282 G allele might influence the binding affinity to DNA elements and alter expression of PPARG-target genes and could then decrease transcriptional activation of the PPARG gene in vitro.8,41 It has been suggested that PPARG rs1801282 C→G substitution could improve insulin sensitivity and decrease body mass index (BMI) and susceptibility of T2DM.42,43 As such, it is believed that PPARG rs1801282 C>G polymorphism may decrease cancer susceptibility through insulin-related mechanisms. In the present case–control study, we found that PPARG rs1801282 C>G polymorphism decreased the risk of HCC. Several meta-analyses found that this SNP decreased the susceptibility of colorectal cancer in Caucasians.44,45 Our findings were similar to those results. In future, more case– control studies are needed to confirm our findings and assess the interaction of genetic predisposition with environmental factors. Rs3856806 C>T polymorphism, located in PPARG exon 6, is correlated with higher BMI.17 A C→T substitution is a synonymous SNP that encodes a histidine amino-acid residue in PPARG protein with either the rs3856806 C or T allele. PPARG rs3856806 C>T variants could influence energy metabolism and then presumably confer risk of T2DM.46 The association between this SNP and the risk of cancer is unknown. Recently, some case–control studies found positive signals of PPARG rs3856806 C>T variants with the development of malignancy.21,47,48 The results of a meta-analysis suggested that PPARG rs3856806 C>T variants did not alter the susceptibility of cancer;49 however, only four case–control studies with small samples were included in this pooled analysis. In this study, we found that PPARG rs3856806 C>T polymorphism was associated with an increased risk of HCC. It was proposed that synonymous SNPs may affect mRNA stability/structure, splicing accuracy, and codon usage.50,51 In combination with our findings, these results showed that PPARG rs3856806 C>T variants might be a risk factor for HCC, probably through altering mRNA processing or translation and influencing the expression of PPARG. Therefore, in future, the function of PPARG rs3856806 C>T polymorphism needs to be explored further. In this study, after Bonferroni correction, genotype distributions of PPARG rs1801282 C>G and rs3856806 C>T polymorphisms were still significantly different between HCC cases and controls, which indicated that our results were reliable. In addition, the results of power analysis also confirm the stability of our findings. We constructed seven haplotypes of PPARG rs1801282 C>G, PPARG rs3856806 C>T, PPARGC1A rs2970847 C>T, PPARGC1B rs7732671 G>C, and PPARGC1B rs17572019 G>A polymorphisms to evaluate the potential inherited patterns of haplotype. We found that CTCGG, CTTGG, and CCCCA haplotypes might increase the susceptibility to HCC. In addition, PPARG Crs1801282Trs3856806 and Grs1801282Crs3856806 haplotypes also influenced the risk of HCC. To the best of our knowledge, this study is the first investigation to explore the association of haplotypes in PPARG rs1801282 C>G, PPARG rs3856806 C>T, PPARGC1A rs2970847 C>T, PPARGC1B rs7732671 G>C, and PPARGC1B rs17572019 G>A polymorphisms with HCC susceptibility. Just as with all epidemiological case–control studies, some limitations should be acknowledged. Firstly, this case–control study was hospital-based. All HCC cases and cancer-free controls were included from eastern China hospitals. Although the minor-allele frequency in controls was very close to data of Chinese populations (Table 2), the selection bias could not have been avoided. Secondly, we selected only five functional polymorphisms based on the publications, which could not represent an extensive view of these genetic predisposition in PPARG, PPARGC1A, and PPARGC1B genes. In future, a fine-mapping case–control study is needed to explore the potential relationships of PPARG, PPARGC1A, and PPARGC1B SNPs with HCC risk further. Thirdly, samples were moderate or small in some subgroups, and the power of the study might be limited in stratification analyses. Fourthly, for lack of sufficient samples, a replication study was not performed. Fifthly, as the distribution of PPARG rs3856806 C>T genotype frequencies did not accord with HWE in controls, our findings should be interpreted with much caution. Finally, due to lack of data for BMI, family history of HCC, other environmental factors, and lifestyle, these potential risk factors were not considered in our study. In future, large-sample studies with detailed individual data are needed to confirm our results. In summary, to the best of our knowledge, this study is the first to explore the relationship of PPARG, PPARGC1A, and PPARGC1B polymorphisms with HCC risk. Our findings suggest that PPARG polymorphisms may influence susceptibility to HCC. In addition, PPARG, PPARGC1A, and PPARGC1B haplotypes were associated with HCC risk. Acknowledgments We appreciate all subjects who participated in this study. We wish to thank Dr Yan Liu (Genesky Biotechnologies Inc, Shanghai, China) for technical support. This study was supported in part by the Clinical Medicine Science and Technology Development Fund of Jiangsu University (JLY20140012), the Fujian Provincial Health and Family Planning Research Talent Training Program (Grant No. 2015-CX-7, 2018-ZQN-13, 2016-1-11, 2018-1-1), the Joint Funds for the Innovation of Science and Technology, Fujian province (Grant No. 2017Y9077), and the National Clinical Key Specialty Construction Program. Disclosure The authors report no conflicts of interest in this work. Table 1 Distribution of selected demographic variables and risk factors in HCC cases and controls Variable Cases (n=584) Controls (n=923) P-valuea n % n % Mean age (years) 53.17 (±11.76) 53.72 (±9.97) 0.327 Age (years) 0.358  <53 264 45.21 395 42.80  ≥53 320 54.79 528 57.20 Sex 0.717  Male 525 89.90 835 90.47  Female 59 10.10 88 9.53 Smoking status 0.834  Never 374 64.04 596 64.57  Ever 210 35.96 327 35.43 Alcohol use <0.001  Never 414 70.89 775 83.97  Ever 170 29.11 148 16.03 Chronic HBV infection <0.001  Yes 412 70.55 85 9.21  No 172 29.45 838 90.79 BCLC classification  A 392 67.12  B 175 29.97  C 17 2.91 Note: a Two-sided χ2-test and Student’s t-test. Abbreviations: BCLC, Barcelona Clinic Liver Cancer; HBV, hepatitis B virus; HCC, hepatocellular carcinoma. Table 2 Primary information for PPARG rs1801282 C>G, rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C, rs17572019 G>A polymorphisms Genotyped SNPs PPARG rs1801282 C>G PPARG rs3856806 C>T PPARGC1A rs2970847 C>T PPARGC1B rs7732671 G>C PPARGC1B rs17572019 G>A Chromosome 3 3 4 5 5 Function Missense Coding-synonymous Coding-synonymous Missense Missense “Chr Pos” (NCBI build 37) 12393125 12475557 23815924 149212243 149212471 MAF for Chinese in database 0.07 0.25 0.28 0.09 0.07 MAF in our controls (n=923) 0.05 0.22 0.22 0.06 0.06 P-value for HWE test in our controls 0.883 0.009 0.498 0.241 0.543 Genotyping method SNP scan SNP scan SNP scan SNP scan SNP scan Percentage genotyping value 99.27% 99.27% 99.27% 99.27% 99.27% Abbreviations: MAF, minor-allele frequency; HWE, Hardy–Weinberg equilibrium; SNP, single-nucleotide polymorphism. Table 3 Logistic regression analyses of associations between PPARG rs1801282 C>G, rs3856806 C>T, PPARGC1A rs2970847 C>T, and PPARGC1B rs7732671 G>C, rs17572019 G>A polymorphisms and risk of HCC Genotype HCC cases (n=584) Controls (n=923) Crude OR (95% CI) P-value Adjusted ORa (95% CI) P-value n % n % PPARG rs1801282 C>G  CC 542 94.26 823 89.36 1.00 1.00  CG 30 5.22 95 10.31 0.47 (0.31–0.72) 0.001 0.47 (0.27–0.82) 0.007  GG 3 0.52 3 0.33 1.50 (0.30–7.45) 0.622 1.97 (0.28–14.13) 0.500  GC + GG 33 5.74 98 10.64 0.51 (0.34–0.77) 0.001 0.52 (0.31–0.88) 0.015  CC + GC 572 99.48 918 99.67 1.00 1.00  GG 3 0.52 3 0.33 1.61 (0.32–7.98) 0.563 2.07 (0.29–14.86) 0.467  C allele 1,114 96.87 1,741 94.52 1.00  G allele 36 3.13 101 5.48 0.56 (0.38–0.82) 0.003 PPARG rs3856806 C>T  CC 320 55.65 543 58.96 1.00 1.00  CT 214 37.22 346 37.57 1.03 (0.82–1.28) 0.828 1.11 (0.83–1.48) 0.483  TT 41 7.13 32 3.47 2.12 (1.31–3.44) 0.002 2.33 (1.25–4.36) 0.008  CT + TT 255 44.35 378 41.04 1.15 (0.93–1.41) 0.208 1.23 (0.93–1.62) 0.145  CC + CT 534 92.87 889 96.53 1.00 1.00  TT 41 7.13 32 3.47 2.13 (1.33–3.43) 0.002 2.26 (1.22–4.17) 0.010  C allele 854 74.26 1,432 77.74 1.00  T allele 296 25.74 410 22.26 1.21 (1.02–1.44) 0.029 PPARGC1A rs2970847 C>T  CC 356 61.91 557 60.48 1.00 1.00  CT 194 33.74 323 35.07 0.92 (0.74–1.15) 0.460 0.98 (0.73–1.31) 0.869  TT 25 4.35 41 4.45 0.93 (0.56–1.56) 0.794 1.25 (0.64–2.43) 0.520  CT + TT 219 38.09 364 39.52 0.94 (0.76–1.17) 0.580 1.02 (0.77–1.35) 0.907  CC + CT 550 95.65 880 95.55 1.00 1.00  TT 25 4.35 41 4.45 0.98 (0.59–1.62) 0.924 1.27 (0.65–2.45) 0.483  C allele 906 78.78 1,437 78.01 1.00  T allele 244 21.22 405 21.99 0.96 (0.80–1.14) 0.619 PPARGC1B rs7732671 G>C  GG 497 86.43 819 88.93 1.00 1.00  GC 77 13.39 101 10.97 1.24 (0.90–1.70) 0.188 1.27 (0.83–1.94) 0.275  CC 1 0.17 1 0.11 1.62 (0.10–26.00) 0.732 1.07 (0.02–49.52) 0.971  GC + CC 78 13.57 102 11.07 1.26 (0.92–1.73) 0.150 1.28 (0.84–1.95) 0.256  GG + GC 574 99.83 920 99.89 1.00 1.00  CC 1 0.17 1 0.11 1.60 (0.10–25.68) 0.739 1.06 (0.02–48.26) 0.977  G allele 1,071 93.13 1,739 94.41 1.00  C allele 79 6.87 103 5.59 1.25 (0.92–1.69) 0.155 PPARGC1B rs17572019 G>A  GG 496 86.26 818 88.82 1.00 1.00  GA 78 13.57 101 10.97 1.25 (0.92–1.72) 0.160 1.27 (0.83–1.94) 0.268  AA 1 0.17 2 0.22 0.81 (0.07–8.98) 0.865 0.78 (0.03–22.31) 0.885  GA + AA 79 13.74 103 11.18 1.27 (0.92–1.73) 0.142 1.28 (0.84–1.94) 0.258  GG + GA 574 99.83 919 99.78 1.00 1.00  AA 1 0.17 2 0.22 0.80 (0.07–8.85) 0.856 0.77 (0.03–21.71) 0.876  G allele 1,070 93.04 1,737 94.30 1.00  A allele 80 6.96 105 5.70 1.24 (0.92–1.67) 0.165 Note: a Adjusted for age, sex, smoking status, alcohol use, and chronic HBV infection status. Bold represents statistically significant values (P<0.05). Abbreviations: HCC, hepatocellular carcinoma; HBV, hepatitis B virus. Table 4 Stratified analyses between PPARG rs1801282 C>G polymorphism and HCC risk by chronic HBV infection, sex, age, smoking status, and alcohol consumption Case/controla Adjusted ORb (95% CI), P-value CC GC GG GC/GG CC GC GG GC/GG GG vs (GC/CC) Sex  Male 486/741 30/89 1/3 31/92 1.00 0.52 (0.29–0.92), P=0.025 0.37 (0.02–6.53), P=0.498 0.52 (0.29–0.91), P=0.022 0.39 (0.02–6.88), P=0.523  Female 56/82 0/6 2/0 2/6 1.00 – – 0.67 (0.12–3.82), P=0.651 – Age (years)  <53 244/360 14/31 2/2 16/33 1.00 0.69 (0.29–1.62), P=0.389 3.07 (0.29–32.72), P=0.352 0.80 (0.36–1.81), P=0.595 3.16 (0.30–33.49), P=0.339  ≥53 298/463 16/64 1/1 17/65 1.00 0.36 (0.17–0.74), P=0.006 1.09 (0.03–40.25), P=0.961 0.38 (0.19–0.77), P=0.007 1.19 (0.03–43.65), P=0.924 Smoking status  Never 350/532 15/59 3/3 18/62 1.00 0.32 (0.15–0.66), P=0.002 2.08 (0.30–14.42), P=0.459 0.39 (0.20–0.77), P=0.007 2.21 (0.32–15.24), P=0.421  Ever 192/291 15/36 0/0 15/36 1.00 0.87 (0.37–2.04), P=0.743 – 0.87 (0.37–2.04), P=0.743 – Alcohol use  Never 386/692 19/79 3/2 22/81 1.00 0.40 (0.21–0.76), P=0.005 3.43 (0.40–29.32), P=0.261 0.47 (0.26–0.86), P=0.015 3.64 (0.43–30.96), P=0.238  Ever 156/131 11/16 0/1 11/17 1.00 0.79 (0.26–2.35), P=0.667 – 0.75 (0.26–2.22), P=0.607 – Chronic HBV infection  Yes 380/78 22/7 2/0 24/7 1.00 0.53 (0.21–1.35), P=0.180 – 0.64 (0.25–1.61), P=0.338 –  No 162/745 8/88 1/3 9/91 1.00 0.42 (0.20–0.89), P=0.024 1.22 (0.12–12.21), P=0.867 0.46 (0.22–0.93), P=0.030 1.29 (0.13–12.89), P=0.832 Notes: a Genotyping successful in 584 (98.46%) HCC cases and 923 (99.78%) controls for PPARG rs1801282 C>G; b adjusted for age, sex, smoking status, chronic HBV infection, and alcohol use (besides stratified factors accordingly) in a logistic regression model. Bold represents statistically significant values (P<0.05). Abbreviations: HCC, hepatocellular carcinoma; HBV, hepatitis B virus. Table 5 Stratified analyses between PPARG rs3856806 C>T polymorphism and HCC risk by chronic HBV infection, sex, age, smoking status, and alcohol consumption Case/controla Adjusted ORb (95% CI), P-value CC CT TT CT/TT CC CT TT CT/TT TT vs (CT/CC) Sex  Male 285/491 196/315 36/27 232/342 1.00 1.08 (0.79–1.48), P=0.635 1.89 (0.94–3.81), P=0.074 1.17 (0.86–1.58), P=0.317 1.85 (0.93–3.69), P=0.078  Female 35/52 18/31 5/5 23/36 1.00 1.06 (0.45–2.50), P=0.888 3.99 (0.91–17.44), P=0.066 1.38 (0.62–3.07), P=0.437 3.95 (0.95–16.47), P=0.059 Age (years)  <53 143/230 99/150 18/13 117/163 1.00 1.25 (0.81–1.92), P=0.320 2.30 (0.85–6.20), P=0.100 1.36 (0.89–2.07), P=0.153 2.13 (0.80–5.63), P=0.129  ≥53 177/313 115/196 23/19 138/215 1.00 1.00 (0.68–1.47), P=0.982 2.21 (0.97–5.01), P=0.058 1.11 (0.77–1.61), P=0.577 2.23 (1.00–4.97), P=0.051 Smoking status  Never 204/347 137/222 27/25 164/247 1.00 1.06 (0.75–1.50), P=0.757 2.24 (1.09–4.60), P=0.028 1.19 (0.85–1.65), P=0.317 2.21 (1.09–4.49), P=0.027  Ever 116/196 77/124 14/7 91/131 1.00 1.27 (0.75–2.16), P=0.379 2.78 (0.73–10.55), P=0.133 1.38 (0.82–2.30), P=0.226 2.53 (0.8–9.38), P=0.164 Alcohol use  Never 230/457 149/288 29/28 178/316 1.00 1.05 (0.76–1.44), P=0.779 2.10 (1.05–4.19), P=0.036 1.16 (0.85–1.58), P=0.350 2.08 (1.05–4.11), P=0.035  Ever 90/86 65/58 12/4 77/62 1.00 1.39 (0.72–2.67), P=0.328 3.57 (0.84–15.10), P=0.085 1.54 (0.82–2.91), P=0.178 3.10 (0.76–12.70), P=0.116 Chronic HBV infection  Yes 230/52 147/29 27/4 174/33 1.00 1.01 (0.60–1.71), P=0.971 1.24 (0.40–3.85), P=0.712 1.07 (0.65–1.78), P=0.787 1.26 (0.41–3.84), P=0.686  No 90/491 67/317 14/28 81/345 1.00 1.12 (0.79–1.59), P=0.523 2.44 (1.22–4.88), P=0.012 1.24 (0.89–1.74), P=0.203 2.34 (1.19–4.60), P=0.014 Notes: a Genotyping successful in 584 (98.46%) HCC cases and 923 (99.78%) controls for PPARG rs3856806 C>T; b adjusted for age, sex, smoking status, chronic HBV infection, and alcohol use (besides stratified factors accordingly) in a logistic regression model. Bold represents statistically significant values (P<0.05). Abbreviations: HCC, hepatocellular carcinoma; HBV, hepatitis B virus. Table 6 PPARG, PPARGC1A, PPARGC1B haplotype frequency (%) in cases and controls and HCC risk Cases Controls Crude OR (95% CI) P-value n (%) n (%) PPARG  Crs1801282Crs3856806 847 (73.59) 1,403 (76.17) 1.00  Crs1801282Trs3856806 267 (23.20) 338 (18.35) 1.31 (1.09–1.57) 0.004  Grs1801282Trs3856806 29 (2.52) 72 (3.91) 0.67 (0.43–1.04) 0.069  Grs1801282Crs3856806 8 (0.70) 29 (1.57) 0.46 (0.21–1.00) 0.046 PPARGC1B  Grs7732671Grs17572019 1,070 (93.04) 1,737 (94.30) 1.00  Crs7732671Ars17572019 79 (6.87) 103 (5.59) 1.25 (0.92–1.69) 0.155 PPARGC1A  Crs2970847 906 (78.78) 1,437 (78.01) 1.00  Trs2970847 244 (21.22) 405 (21.99) 0.96 (0.80–1.14) 0.619 PPARG, PPARGC1A, and PPARGC1B  Crs1801282Crs3856806Crs2970847Grs7732671Grs17572019 618 (53.79) 1,044 (56.71) 1.00  Crs1801282Trs3856806Crs2970847Grs7732671Grs17572019 198 (17.23) 259 (14.07) 1.29 (1.05–1.59) 0.017  Crs1801282Crs3856806Trs2970847Grs7732671Grs17572019 159 (13.84) 277 (15.05) 0.97 (0.78–1.21) 0.783  Crs1801282Trs3856806Trs2970847Grs7732671Grs17572019 59 (5.13) 64 (3.48) 1.56 (1.08–2.25) 0.017  Crs1801282Crs3856806Crs2970847Crs7732671Ars17572019 56 (4.87) 58 (3.15) 1.63 (1.11–2.39) 0.011  Grs1801282Trs3856806Crs2970847Grs7732671Grs17572019 21 (1.83) 42 (2.28) 0.84 (0.50–1.44) 0.534  Crs1801282Crs3856806Trs2970847Crs7732671Ars17572019 12 (1.04) 23 (1.25) 0.88 (0.44–1.78) 0.725  Grs1801282Trs3856806Trs2970847Grs7732671Grs17572019 7 (0.61) 26 (1.41) 0.45 (0.20–1.05) 0.060  Others 19 (1.65) 48 (2.61) 0.67 (0.39–1.15) 0.142 Note: Bold represents statistically significant values (P<0.05). 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