==== Front Ann Saudi MedAnn Saudi MedAnnals of Saudi Medicine0256-49470975-4466King Faisal Specialist Hospital and Research Centre 2406072110.5144/0256-4947.2013.394asm-4-394Case ReportBrucella spp isolated from respiratory sample and grown in Mycobacterium growth indicator tube (MGIT) Somily Ali Mohammed aKambal Abdelmageed Mohamed aNaeem Tahir aBabay Hanan Ahmed aAl Hedaithy Mogbil Abdullah bAl Anazi Awadh Raheel bBarry Mazin Adnan bAl Aska Abdulkarim Ibrahim bMorshed Muhammad Golam cMurray Thomas Scot d a Department of Pathology, Microbiology, King Saud University College of Medicine and King Khalid University Hospital, Riyadh, Saudi Arabia b Department of Internal Medicine, Infectious Diseases, and, Riyadh, Saudi Arabia c Department of Pathology, Laboratory Medicine, University of British Columbia, Vancouver, Canada d Department of Laboratory Medicine, Infectious Diseases, College of Medicine, University of Yale, Connecticut, New Haven, United StatesCorrespondence: Dr. Ali Mohammed Somily, Department of Pathology, Microbiology, King Saud University College of Medicine & King Khalid University Hospital, PO Box 2925 Riyadh 11461, Saudi Arabia, ali.somily@gmail.comJul-Aug 2013 33 4 394 396 Copyright © 2013, Annals of Saudi Medicine2013This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.Brucellosis remains endemic in many countries including Saudi Arabia. The disinfection of objects and surfaces contaminated with Brucella spp is not difficult, but we encountered a situation in which the organism survived the decontamination and liquefaction procedure adopted for AFB culture. A sputum specimen from a patient was sent for TB culture and the BACTEC MGIT 960 system isolated an organism identified as Brucella spp. The blood cultures and the serological testing had confirmed this case to be brucellosis. Isolation of Brucella spp from sputum samples is rare; this case appears to be the first of its kind. As the clinical presentation of TB may mimic brucellosis and vice versa, we recommend that handling specimens from all cases of undiagnosed PUO should be done with care because of the possibility that it may contain either of these organisms. ==== Body Brucellosis is a zoonotic disease of worldwide distribution affecting mainly those working with domestic animals and/or their products.1 Although it is controlled in many developed countries, the disease remains endemic in many parts of the world including Saudi Arabia.2 The clinical presentation can vary from asymptomatic infection with seroconversion to systemic disease with fever, night sweats, and musculoskeletal manifestations. Rarely, the liver, heart, and eyes or the central nervous system may be involved.3 Although Brucella spp can survive in dust and dry environments,4 they are considered fastidious organisms because they are susceptible to routine disinfection procedures such as household bleach and 70% ethanol.5 The decontamination procedure was adapted for setting up mycobacterial cultures in the BACTEC MGIT 960 system that is designed to kill all bacteria except Mycobacterium spp.6 We describe a case in which a sputum specimen submitted for mycobacterial culture and decontaminated by the usual procedure grew Brucella spp hence showing the resistance of Brucella spp to both the recommended decontamination procedure and the antibiotics, PANTA (polymyxin B, amphotericin B, nalidixic acid, trimethoprim, azlocillin) in the MGIT tube. CASE On 23 February 2011, a 15-year-old Sudanese male presented to the Accident and Emergency Department of King Khalid University Hospital, Riyadh, Saudi Arabia complaining of fever and chills for seven days. He had history of ingesting raw camel milk on a visit to Sudan two months previously. Fever (38.4°C) and a palpable spleen were the only positive findings on physical examination. The patient had no specific respiratory symptoms or signs. Results of routine investigations were: WBCs 3400/mm3 with 73% lymphocytes and 21% polymorphonuclear leukocytes, and an erythrocyte sedimentation rate of 17 mm at the first hour. Thin and thick blood films for malaria parasites were negative, alkaline phosphatase was 193 U/L (normal range 50–180 U/L), alanine aminotranferase 67 U/L (normal range 20–65 U/L), aspartate aminotranferase 76 U/L (normal range 12–37 U/L). Brucella serology was B abortus titer was 1:2560 while B melitensis titer was 1:320. The urinanalysis was normal and there was no growth from urine culture. Three sets of specimens for blood culture (BacT/Alert System) were submitted to the laboratory. Although the patient had no specific respiratory symptoms or signs, two days later a sputum sample was collected for acid fast bacilli (AFB) smear and mycobacterial culture to rule out pulmonary tuberculosis considering the endemicity of the disease in the area. The sputum specimen was processed in accordance with the laboratory protocol for mycobacteriology culture. Decontamination and liquefaction was performed using 2% NALC-NaOH solution. On the same day a total of 12 specimens were processed using the same procedure. The specimen was then centrifuged and the sediment was inoculated into one tube of liquid MGIT medium containing PANTA supplement and one slope each of Lowenstein-Jensen LJ-glycerol and LJ-pyruvate medium. The MGIT tube was incubated in BACTEC MGIT 960 (Becton, Dickinson and Company Diagnostic Systems, Maryland USA) system and LJ slopes were incubated separately at 37°C. The smear was stained with auramine O fluorescent technique and examined under fluorescent microscope. No AFB were detected in the smear. On February 27, all three sets of blood cultures gave a positive signal. Gram-stain smears from all bottles showed gram-negative coccobacilli morphologically resembling Brucella spp. Subcultures were done onto a solid blood agar plate (BAP), a chocolate agar plate (CAP), and MacConkey agar media. The BAP and CAP yielded growth of organisms subsequently identified as Brucella spp based on positive oxidase and urease tests, along with positive serological typing as Brucella melitensis. After 9 days of incubation in the BACTEC MGIT 960 system, the MGIT tube containing the sputum specimen gave a signal of positivity. The tube was retrieved and processed for smear and subculture in accordance with the manufacturer’s instructions. Smears were stained with Zeihl-Neelsen (ZN) stain for AFB and with Gram stain. Subcultures were done on LJ slopes and on BAP and MacConkey agar plate to rule out contaminants and fast growing mycobacterial species. The ZN stained smear did not show AFB, but the Gram-stained smear showed gram-negative coccobacilli morphologically resembling Brucella spp. The subculture on BAP grew typical colonies that were identified as Brucella melitensis using the same criteria as described above. The batch set up for mycobacterial cultures done on the same day was reviewed and technical errors were excluded as all other specimens remained negative except three specimens from other patients that grew AFB subsequently identified as Mycobacterium tuberculosis. To validate the resistance of the isolate to the decontamination and PANTA, a suspension (1.0 Macfarland) was prepared and subjected to the same decontamination procedure once again. The second exposure to a decontamination procedure and antibiotics contained in the PANTA supplement failed to kill the organism. This further re-enforced our initial observation. The patient was started on streptomycin injections for two weeks and oral doxycycline for four weeks. He was discharged from the hospital after three days, being afebrile, and advised to complete antimicrobial therapy at home. The susceptibility performed on this isolate showed that it was resistant to trimethoprim, but fully susceptible to streptomycin, sulfamethoxazole, tetracycline and rifampicin. DISCUSSION The major routes of Brucella infection to humans are ingestion of dairy products, inhalation of aerosols, and inoculation through the skin or mucous membranes or contact with infected animals and/or their products.7 Although the inhalation route of entry of Brucella into the body is not an uncommon route of transmission, the disease seldom involves the respiratory system and the isolation from sputum is only rarely reported.8 N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) mixture is used for liquefaction and decontamination of the sputum samples before setting up mycobacterial cultures.6 The liquid broth medium for mycobacterial culture is known to yield better recovery and faster growth of mycobacteria.9 The MGIT contains modified Middlebrook 7H9 broth base, and enrichment. The presence of antibiotics in the MGIT tube [polymyxin B (2,000U/ml), amphotericin B (200μg/ml), nalidixic acid (800μg/ml), trimethoprim (200μg/ml), azlocillin (200μg/ml)] is necessary to suppress contamination of culture with other bacteria.10 It was iunexpected that Brucella survived the liquefaction and decontamination steps of setting up mycobacterial cultures and further survived exposure to the PANTA mixture in the MGIT tubes. It was not a case of decontamination failure because the batch of samples processed on the same day remained negative except in this case and the three other specimens that grew mycobacterium subsequently identified as M tuberculosis. In our laboratory, the decontamination process is checked regularly by quality control for each batch and our contamination rate with other respiratory flora matches the international standard. It is of interest to mention that of the five antibiotics used in PANTA, trimethoprim is a component of cotrimoxazole, a recognized therapy for brucellosis. The concentration of trimethoprim in cotrimoxazole tablets is 160 mg while in a study the MIC90 of Brucella spp was found to be 0.125μg/mL11 and the amount present in PANTA is expected to inhibit Brucella spp. In countries where brucellosis and TB remain endemic, brucellosis can mimic clinically TB and sputum specimens processed for M tuberculosis may contain Brucella spp. Brucellosis is still one of the top differential diagnoses of pyrexia of unknown origin in Saudi Arabia and treating physicians should have a high index of suspicion and discuss the case with the clinical microbiology laboratory to collect the best specimen according to the clinical syndrome to avoid a delay in the diagnosis and increased morbidity for the patient. ==== Refs REFERENCES 1 Corbel MJ Brucellosis: An overview Emerging Infectious Diseases 1997 3 213 221 9204307 2 Al-Eissa YA Brucellosis in Saudi Arabia: Past, Present and Future Annals of Saudi Medicine 1999 19 403 5 17277503 3 Young EJ Brucella Species Mandell GL Bennett JE Dolin R Principles and Practice of Infectious Diseases 6th Edition Churchill Livingstone 2005 2669 74 4 European Commission Health and Consumer Protection Directorate General. 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