==== Front Neuropsychiatr Dis TreatNeuropsychiatr Dis TreatNeuropsychiatric Disease and TreatmentNeuropsychiatric Disease and Treatment1176-63281178-2021Dove Medical Press 10.2147/NDT.S164619ndt-14-1919Original ResearchTinnitus distress is associated with enhanced resting-state functional connectivity within the default mode network Chen Yu-Chen 12Chen Huiyou 2Bo Fan 2Xu Jin-Jing 3Deng Yi 3Lv Han 4Cai Yuexin 5Xia Wenqing 6Yin Xindao 2Gu Jian-Ping 2Lu Guangming 1 1 Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China, cjr.luguangming@vip.163.com 2 Department of Radiology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China, cjr.gujianping@vip.163.com 3 Department of Otolaryngology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China 4 Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Beijing, China 5 Department of Otolaryngology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China 6 Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University, Nanjing, ChinaCorrespondence: Jian-Ping Gu, Department of Radiology, Nanjing First Hospital, Nanjing Medical University, No 68, Changle Road, Nanjing 210006, China, Tel +86 25 8772 6268, Fax +86 25 5223 6361, Email cjr.gujianping@vip.163.comGuangming Lu, Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, No 305, Eastern Zhongshan Road, Nanjing 210002, China, Email cjr.luguangming@vip.163.com2018 01 8 2018 14 1919 1927 © 2018 Chen et al. This work is published and licensed by Dove Medical Press Limited2018The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.Purpose The default mode network (DMN) has been confirmed to be involved in chronic tinnitus perception. Tinnitus distress may be associated with abnormal functional connectivity (FC) within the DMN regions. The goal of this study was to determine whether tinnitus disrupted the FC patterns within the DMN as measured by using resting-state functional magnetic resonance imaging approach. Patients and methods Resting-state functional magnetic resonance imaging scans were acquired from 40 chronic bilateral tinnitus patients and 41 healthy controls. Both were age, sex, and education well-matched with normal hearing. Two important DMN regions, the anterior cingulate cortex and posterior cingulate cortex, were chosen as seed regions to detect the FC patterns within the DMN and then determine whether these changes were linked to clinical measures of tinnitus such as tinnitus duration and tinnitus severity. Results Compared with healthy controls, chronic tinnitus patients manifested significantly enhanced FC between the anterior cingulate cortex and left precuneus, which was correlated with the tinnitus duration (r=0.451, p=0.007). Moreover, enhanced FC between the posterior cingulate cortex and right medial prefrontal cortex in tinnitus patients was positively correlated with the tinnitus distress (r=0.411, p=0.014). Conclusion Chronic tinnitus patients showed disrupted FC patterns within the DMN regions which are correlated with tinnitus distress. Increased resting-state connectivity pattern of the DMN may play a pivotal role in neuropathological features underlying chronic tinnitus. Keywords tinnitusanterior cingulate cortexposterior cingulate cortexdefault mode networkresting-state fMRI ==== Body Introduction Tinnitus is an auditory phantom percept of sound in the absence of an external source.1,2 Of the 20 million people with tinnitus in USA, 2 million are extremely bothered and debilitated by the perception.3–5 Tinnitus patients often suffer from sleep disturbances, depression, anxiety, and other comorbid conditions that significantly impair the quality of daily life.5–8 Prior studies have suggested that chronic tinnitus is associated with various neuropathological changes, such as enhanced spontaneous neuronal activity, aberrant consciousness gating, tonotopic map reorganization, increased neural synchrony, and coupling of networks involving auditory and non-auditory structures.9–14 Nevertheless, the neural mechanisms that maintain this disorder still remain poorly understood. Resting-state functional magnetic resonance imaging (fMRI) has become a novel and widely used neuroimaging technique to explore the potential pathogenesis of various neuropsychiatric disorders. It is sensitive to low-frequency (0.01–0.1 Hz) spontaneous fluctuations in the blood oxygenation level-dependent (BOLD) signal with high spatial resolution and easy application, which represents baseline spontaneous neuronal activity.15–17 Moreover, resting-state networks (RSNs) are defined as brain regions with highly correlated time courses of low-frequency (<0.1 Hz) BOLD signal fluctuations during the baseline state.18 Prior studies have applied resting-state fMRI to examine multiple whole-brain RSNs, such as auditory network, attention network, and default mode network (DMN), to study the neural mechanisms of tinnitus.14,19,20 Among them, the DMN is most active at rest and shows reduced activity when a subject enters a task-based state involving attention or goal-directed behavior, which consists of nodes in the anterior cingulate cortex (ACC), posterior cingulate cortex (PCC)/precuneus, medial prefrontal cortex (mPFC), and inferior parietal lobule (IPL).21 Because of its persistent nature, some have proposed that the generation of tinnitus may be associated with the abnormalities in the DMN.19 Schmidt et al observed that the precuneus was found to have significantly decreased connectivity with other nodes of the DMN in tinnitus patients relative to control groups.22,23 Other studies using independent component or graph theory analyses have demonstrated enhanced functional connectivity (FC) between the DMN and other brain networks in tinnitus patients.24,25 Enhanced FC was also observed between the DMN and other brain regions such as limbic system, which was associated with tinnitus distress.26 Moreover, other psychiatric disorders, such as posttraumatic stress disorder27 or Parkinson’s disease,28 have also observed the associations between elevated connectivity within the DMN and disease-related distress. Thus, it is not surprising that this specific association is implicated in tinnitus as well. Furthermore, as the core nodes of DMN, the ACC and PCC play important roles in emotional control and processing distressing information.29,30 During cognitive processing, the PCC is functionally related to ventral ACC and the DMN-related brain region.30 Thus, the FC alterations of ACC and PCC might be associated with the cognitive deficit of tinnitus patients. Our previous studies have detected increased spontaneous neural activity in specific DMN regions of tinnitus including ACC and PCC.31–33 The DMN is relatively better connected internally (ie, larger spontaneous fluctuations, indicative of stronger connectivity) when the mind is at rest and allowed to wander or daydream.21 Nevertheless, none of the current studies have specifically revealed FC alterations within the DMN in chronic tinnitus. Disruptions of the DMN are not exclusive to tinnitus. Previous studies have noted FC disruptions primarily in anterior-to-posterior connections within the DMN in Alzheimer’s disease.34,35 Moreover, results from studies of the DMN in major depressive disorder indicate a hyperactive network with increased FC changes that are associated with the process of rumination.36 However, the source of abnormal neural activity within specific DMN regions related to tinnitus still remains elusive. Considering the crucial role of the DMN in the neuropathology of tinnitus, we aimed to employ a seed-based approach to investigate the intrinsic FC within the DMN regions between tinnitus patients with normal hearing and healthy controls. We hypothesized that resting-state FC patterns within the DMN regions in tinnitus would be significantly distinct from controls and that the enhanced FC within the DMN would correlate with specific tinnitus characteristics such as tinnitus distress and tinnitus duration. Patients and methods Participants This study was approved by the Research Ethics Committee of the Nanjing Medical University. All individuals provided written informed consent before their participation in the study protocol. A total of 40 bilateral tinnitus patients were recruited for this study at the Department of Otolaryngology of Nanjing First Hospital. Forty-one age-, sex-, and education-matched healthy controls were recruited through community health screening or newspaper advertisement. All participants were right-handed and completed at least 8 years of education. None of the participants was excluded because of exceeded limits for head motion during scanning. Tinnitus distress was assessed by the Iowa version of the Tinnitus Handicap Questionnaires (THQ).37 Based on the THQ score and previously proposed guidelines,38 the severity of the tinnitus patients was categorized as mild, moderate, or severe. Twenty patients had mild tinnitus, 17 had moderate tinnitus, and three had severe tinnitus in this study. Hearing thresholds were determined by pure tone audiometry (PTA) at the frequencies of 0.25, 0.5, 1, 2, 4, and 8 kHz. None of the participants had hearing loss in any of the six measured audiometric frequencies (average PTA ≤25 dB). There were no statistically significant differences in hearing thresholds between the two groups (Figure 1; Table 1). Depression and anxiety status were assessed according to the Self-Rating Depression Scale (SDS) and Self-Rating Anxiety Scale (SAS).39,40 According to a previous study,41 the Hyperacusis Questionnaire was used to exclude patients with hyperacusis in this study. Participants were also excluded if they suffered from pulsatile tinnitus, hyperacusis, Meniere’s diseases, or had a past history of otologic surgery, ototoxic drug therapy, noise exposure, or hearing aid use, severe smoking, stroke, alcoholism, brain injury, Parkinson’s disease, Alzheimer’s disease, epilepsy, major depression, neurological or psychiatric disorders that could affect cognitive function, major medical illness (eg, anemia, thyroid dysfunction, and cancer), MRI contraindications, or severe visual loss. The characteristics of the tinnitus patients and healthy controls are summarized in Table 1. MR acquisition MRI data were acquired by using a 3.0 T MRI scanner (Ingenia; Philips Medical Systems, the Netherlands) with an eight-channel receiver array head coil. Head motion and scanner noise were alleviated by using foam padding and earplugs. The earplugs (Hearos Ultimate Softness Series, Aliso Viejo, CA, USA) were used to attenuate scanner noise by ~32 dB based on the manufacture’s data sheet. The participants were instructed to lie down quietly with their eyes closed and without falling asleep, to not think about anything in particular, and to avoid any head motion during the scan. Functional images were obtained axially by using a gradient echo-planar imaging sequence as follows: repetition time (TR) =2,000 ms; echo time (TE) =30 ms; slices =36; thickness =4 mm; gap =0 mm; field of view (FOV) =240×240 mm; acquisition matrix =64×64; and flip angle (FA) =90°. The fMRI sequence took 8 minutes and 8 seconds. All scans were acquired with parallel imaging by using sensitivity encoding (SENSE) technique, SENSE factor =2. Structural images were acquired with a three-dimensional turbo fast echo T1WI sequence with high resolution as follows: TR/TE =8.1/3.7 ms; slices =170; thickness =1 mm; gap =0 mm; FA =8°; acquisition matrix =256×256; FOV =256×256 mm2. The structural sequence took 5 minutes and 29 seconds. Data preprocessing fMRI data preprocessing was carried out by using the software tool Data Processing Assistant for Resting-State fMRI programs,42 which is based on Statistical Parametric Mapping (SPM12, http://www.fil.ion.ucl.ac.uk/spm) and the resting-state fMRI data analysis toolkit (REST, http://www.restfmri.net). The first 10 volumes were removed from each time series to account for the time it took participants to adapt to the scanning environment. Slice timing and realignment for head-motion correction were then performed for the remaining 230 images. Participant data exhibiting head motion >2.0 mm translation or >2.0° rotation were excluded from the analysis. The remaining data set was spatially normalized to the Montreal Neurological Institute template (resampling voxel size =3×3×3 mm3), smoothing with an isotropic Gaussian kernel (full width at half maximum [FWHM] =6 mm), detrending and filtering (0.01–0.08 Hz) were performed in order. Structural data analysis Structural images of each subject were processed by using the VBM toolbox software in SPM12 (http://www.fil.ion.ucl.ac.uk/spm). Briefly, the structural images were normalized and segmented into gray matter (GM), white matter (WM), and cerebrospinal fluid (CSF) by using the unified segmentation model in SPM12.43 Brain parenchyma volume was calculated as the sum of GM and WM volumes. T1 images were normalized to the MNI template by using affine linear registration followed by Gaussian smoothing (FWHM =8 mm). Functional data analysis The seed regions of interest (ROIs) of the ACC and PCC were generated from Brodmann template by using the WFU_PickAtlas software.44 Briefly, the mean time series of each ROI was obtained for reference time course. Then, the Pearson’s correlation coefficients were calculated between the mean signal change of each ROI and the time series of each voxel. Finally, a Fisher’s z-transform was used to improve the normality of the correlation coefficients.45 Six head motion parameters and mean time courses of global, WM, and CSF signals were included in the regression analysis. One-sample t-test was performed on the individual FC maps in a voxel-wise manner. Each individual’s z map was entered into the SPM12 software for a random effect one-sample t-test to determine the brain regions showing significant FC to ACC and PCC. Significant thresholds were corrected by using false discovery rate (FDR) criterion and set at p<0.001, based on the suggestion from a prior study.46 Two-sample t-tests were performed to investigate FC differences between tinnitus patients and controls by using a default whole-brain mask. Age, sex, education, GM volume, and mean hearing thresholds were used as nuisance covariates. The voxel-wise results of group difference were corrected for multiple comparisons by using FDR criterion and the significance set at p<0.001. Statistical analysis Between-group t-tests and χ2 tests were used to analyze the differences in the demographic data between tinnitus patients and healthy controls. To identify the relationship between abnormal FC and clinical variables, Pearson correlation analyses were performed in a ROI-wise manner. The mean z-values of each brain region that showed significant group differences were extracted within every subject. Pearson correlation analysis between the mean z-values and each clinical variable was performed by using SPSS 19.0 (SPSS Inc., Chicago, IL, USA). Statistical threshold was set at p<0.05. Partial correlations between extracted values and clinical variables were calculated using the SPSS software. Analyses were adjusted for age, sex, education, GM volume, mean hearing thresholds, and specific clinical variables. Bonferroni correction was applied for multiple comparisons in the correlation analyses. The Bonferroni corrected p-value was 0.0125. Since micromovements from volume to volume can influence the FC,47 framewise displacement (FD) values were computed for each subject to reflect the temporal derivative of the movement parameters. No subjects had FD >0.5 mm on more than 35 volumes in this study. No significant difference was found in the mean FD values between tinnitus patients and controls (Table 1). Results Structural results Table 1 shows no significant differences in GM and WM volumes in tinnitus patients compared with healthy controls (p>0.05). After Monte Carlo simulation correction, no suprathreshold voxel-wise difference in the GM and WM volume between tinnitus patients and healthy controls was observed. Functional analysis The ACC and PCC mainly showed positive FC within the DMN regions, including the mPFC, IPL, and precuneus in both the tinnitus patients (Figure 2A) and healthy controls (Figure 2B). Relative to healthy controls, bilateral tinnitus patients exhibited significantly enhanced FC between the ACC and left precuneus as well as increased FC between PCC and right mPFC (Figure 3; Table 2). Correlation analysis results In bilateral tinnitus patients, the enhanced FC of ACC–left precuneus was positively correlated with the tinnitus duration (r=0.455, p=0.007) (Figure 4A; Table 3), which could persist after Bonferroni correction. Furthermore, the increased FC of the PCC to right mPFC was positively associated with the THQ score (r=0.381, p=0.026) (Figure 4B; Table 3), which could not persist after Bonferroni correction, probably partly due to the relatively strict calculation. These correlations had been corrected for age, sex, education, GM volume, and mean hearing thresholds. None of the increased FC was correlated with SAS or SDS score (Table 3). Discussion In the current study, we used the seed-based method to investigate disrupted DMN patterns in patients with bilateral chronic tinnitus. Compared with healthy subjects, chronic tinnitus patients revealed significantly enhanced FC between the ACC and left precuneus, which was correlated with the tinnitus duration. Moreover, enhanced FC between the PCC and right mPFC in tinnitus patients was positively correlated with the tinnitus distress. In this study, we compared GM and WM volumes but did not detect any brain structural changes between the normal hearing tinnitus patients and matched healthy controls, which was consistent with our prior studies.26,31–33,48,49 By using VBM or surface-based morphometry, prior studies have observed decreases of GM volume in tinnitus patients in several brain regions including ACC, mPFC, inferior colliculus, hippocampus, superior frontal gyrus, occipital lobe, and hypothalamus.50–58 Increases in GM volume have also been reported in tinnitus patients in the superior and middle temporal gyrus.57 The possible explanation for this is the absence of hearing loss over the extended frequency range and absence of hyperacusis in our tinnitus patients. Moreover, the MR analytical technique could also contribute to the discrepancy. Patients with chronic tinnitus had significantly greater FC between the ACC and left precuneus, which was correlated with tinnitus duration. We suggest that the ACC is part of the core structure of anterior DMN, which may show functional dysconnectivity with the precuneus in tinnitus. Precuneus is a highly integrated structure, supposed to be involved in self-consciousness, shifting of attention, auditory memory retrieval, auditory imagery, and memory-related aspects of the tinnitus perception.59,60 Moreover, precuneus is one of the important regions in brain that are linked with tinnitus distress.19,61–63 However, in this study, the patients maintained higher connectivity with the left precuneus that was correlated with tinnitus duration, indicating preservation of normal episodic memory, despite the presence of persistent tinnitus percept.64,65 Furthermore, some research studies show that a positive correlation between the strength of inflow to the temporal cortex and tinnitus distress was found in DMN areas, especially in the precuneus.66 Despite the correlation with the tinnitus duration or tinnitus distress, these results all imply that the aberrant activity of precuneus within the DMN may be a potential marker of chronic tinnitus. Our current evidence suggests that the elevated connectivity within the DMN is a long-term effect and therefore a consequence (not a cause) from chronic tinnitus. Previous studies have also demonstrated aberrant FC changes exclusively within the DMN in patients with long-term tinnitus compared with healthy controls,22,23,67 suggesting that this disruption within the DMN is not immediate but occurs over time in tinnitus patients. In addition, the existing literature seems to indicate that tinnitus severity has a significant impact on the brain resting-state FC networks within the DMN.19,24,25,68 Besides that, the precuneus has also been suggested to be related to conscious and internal awareness and may play a key role in tinnitus generation and persistence.69,70 Therefore, consistent with the aforementioned studies, our results suggest that abnormal FC between the ACC and precuneus may be responsible for disrupting the DMN in chronic tinnitus patients. Nevertheless, the source of abnormal neural activity within specific DMN regions due to tinnitus still remains unknown. Alongside impaired connectivity in the precuneus, patients with chronic tinnitus also showed increased connectivity between the PCC and mPFC. This finding is similar to prior fMRI studies that have reported enhanced connectivity in the mPFC among tinnitus patients.14,26,33,54,71–73 The mPFC has been regarded as a pivotal region that integrates sensory and emotional aspects of tinnitus.74 Rauschecker et al developed a model to identify structural and functional differences in ventromedial prefrontal cortex which were related to tinnitus loudness, indicating the contribution of mPFC to certain perceptual features of tinnitus.71 Moreover, Araneda et al indicated that deficit in executive functions caused by mPFC alterations might be a key factor in the generation and maintenance of tinnitus.72 Ahn et al suggested that tinnitus might be related to impaired prefrontal top-down inhibitory control using resting-state electroencephalography.75 Moreover, increased FC strength between auditory and PFC regions was observed through resting-state functional near-infrared spectroscopy.76 In addition, our previous study also observed significantly enhanced FC within the executive control of attention network involving the mPFC region.32 However, in other studies with larger sample size, chronic tinnitus-related distress was not correlated with morphometric or functional changes in the PFC.50,51,58,77 The heterogeneity of the tinnitus population and the different MR techniques may contribute to the discrepancies. Thus, our findings of increased FC between the posterior DMN region and mPFC might suggest that the mPFC serves as a main cortical hub in the brain network influenced by chronic tinnitus. There were some limitations in the current study. First, this is a cross-sectional study with a limited sample size. Further longitudinal investigations are needed to confirm the relationships between aberrant FC patterns within the DMN and developing tinnitus distress in future study. Second, the scanner noise cannot be completely eliminated even with earplugs, which may reduce the differences in RSNs between tinnitus patients and controls. This confounding factor should be taken into consideration in future studies. Moreover, besides the functional dysconnectivity, more research studies are needed to demonstrate the possibility of structural connectivity within the DMN that can be detected by using the diffusion tensor imaging approach. Besides the VBM analysis, surface-based methods such as FreeSurfer or related methods may be more precise and informative for providing cortical thickness, surface area, and volume, which need to be considered in our future study. Finally, subtypes of different age, severity, and other confounding factors that could result in a deviation of tinnitus require to be divided to prevent the disordered effect of the inconformity of these subgroups. Conclusion Despite these limitations, this study demonstrated enhanced resting-state FC patterns within the DMN, which was associated with the tinnitus-related distress. These findings mainly illustrated the potential role of DMN in chronic tinnitus patients which will contribute to a better understanding of the neuropathological mechanisms in tinnitus perception. Acknowledgments This work was supported by a grant from the National Natural Science Foundation of China (no 81601477), Natural Science Foundation of Jiangsu Higher Education Institutions (no 16KJB320001), Medical Science and Technology Development Foundation of Nanjing Department of Health (no YKK16140), Youth Medical Talents of Jiangsu Province (no QNRC2016062), China Postdoctoral Science Foundation (no 2017M610337), Jiangsu Postdoctoral Science Foundation (no 1701007A), 14th “Six Talent Peaks” Project of Jiangsu Province (no YY-079), and Nanjing Outstanding Youth Fund (no JQX17006). Disclosure The authors report no conflicts of interest in this work. Figure 1 No significant differences in auditory thresholds between tinnitus and control groups. Note: Data are presented as mean±SEM. Abbreviation: SEM, standard error of the mean. Figure 2 Significant FC patterns from the ROIs of the (A) ACC and (B) PCC in whole brain by using one-sample t-test in both chronic tinnitus patients and healthy controls. Note: Significant thresholds were corrected by using FDR criterion and set at p<0.001. Abbreviations: FC, functional connectivity; ROI, region of interest; ACC, anterior cingulate cortex; PCC, posterior cingulate cortex; FDR, false discovery rate. Figure 3 Aberrant FC of the (A) ACC and (B) PCC in tinnitus patients compared with healthy controls. Note: The threshold was set at a p<0.001 (FDR corrected). Abbreviations: FC, functional connectivity; ACC, anterior cingulate cortex; PCC, posterior cingulate cortex; FDR, false discovery rate. Figure 4 Significant correlations between the enhanced FC and tinnitus characteristics. Note: (A) Positive correlation between the increased FC of the ACC–left precuneus and the tinnitus duration. (B) Positive correlation between the increased FC of the PCC–right mPFC and the THQ score. Abbreviations: FC, functional connectivity; ACC, anterior cingulate cortex; PCC, posterior cingulate cortex; mPFC, medial prefrontal cortex; THQ, Tinnitus Handicap Questionnaires. Table 1 Characteristics of tinnitus patients and healthy controls Tinnitus patients (n=40) Healthy controls (n=41) p-value Age (years) 53.6±12.5 49.5±10.5 0.110 Male/female 19/21 16/25 0.441 Education levels (years) 12.5±2.4 12.7±2.9 0.670 Tinnitus duration (months) 41.9±34.5 – – THQ score 52.1±15.9 – – SDS score 41.8±5.6 40.0±4.6 0.123 SAS score 39.6±6.5 37.0±5.6 0.066 Gray matter 578.8±25.6 577.5±22.7 0.815 White matter 534.6±21.8 526.7±25.6 0.139 Brain parenchyma 1,113.4±32.1 1,104.2±35.4 0.225 Hearing thresholds (left) 15.8±3.5 16.7±2.5 0.093 Hearing thresholds (right) 166±3.4 17.0±2.2 0.592 Hearing thresholds (average) 16.1±3.0 16.8±1.5 0.158 FD value (mm) 0.21±0.06 0.21±0.07 0.952 Note: Data are represented as mean±SD. Abbreviations: THQ, Tinnitus Handicap Questionnaire; SDS, Self-Rating Depression Scale; SAS, Self-Rating Anxiety Scale; FD, framewise displacement. Table 2 Abnormal functional connectivity of ACC and PCC in bilateral tinnitus patients compared with healthy controls Brain region BA MNI coordinates x, y, z (mm) T score Voxels ACC L precuneus 7 −17, −64, 46 3.4215 80 PCC R medial prefrontal cortex 10 15, 60, 24 3.4359 62 Note: The threshold was set at a p<0.001 (FDR corrected). Abbreviations: ACC, anterior cingulate cortex; PCC, posterior cingulate cortex; BA, Brodmann’s area; MNI, Montreal Neurological Institute; L, left; R, right; FDR, false discovery rate. Table 3 Correlation coefficients between the increased functional connectivity of ACC and PCC and different tinnitus characteristics Brain region Tinnitus duration THQ SDS SAS ACC–left precuneus r=0.455, p=0.007* r=0.078, p=0.661 r=−0.082, p=0.690 r=−0.336, p=0.094 PCC–right mPFC r=−0.115, p=0.518 r=0.381, p=0.026* r=−0.028, p=0.891 r=0.296, p=0.142 Notes: Partial correlations were controlled for age, sex, education, GM volume, and mean hearing thresholds. * p<0.05. Abbreviations: ACC, anterior cingulate cortex; PCC, posterior cingulate cortex; mPFC, medial prefrontal cortex; THQ, Tinnitus Handicap Questionnaires; SDS, Self-Rating Depression Scale; SAS, Self-Rating Anxiety Scale. ==== Refs References 1 Wegger M Ovesen T Larsen DG Acoustic coordinated reset neuromodulation: a systematic review of a novel therapy for tinnitus Front Neurol 2017 8 36 28243221 2 Møller AR Tinnitus: presence and future Prog Brain Res 2007 166 3 16 17956767 3 Henry JA Dennis KC Schechter MA General review of tinnitus: prevalence, mechanisms, effects, and management J Speech Lang Hear Res 2005 48 1204 1235 16411806 4 Hall DA Lainez MJ Newman CW Treatment options for subjective tinnitus: self reports from a sample of general practitioners and ENT physicians within Europe and the USA BMC Health Serv Res 2011 11 302 22053947 5 Langguth B Kreuzer PM Kleinjung T De Ridder D Tinnitus: causes and clinical management Lancet Neurol 2013 12 920 930 23948178 6 Reynolds P Gardner D 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