==== Front Eur J HistochemEJHEuropean Journal of Histochemistry : EJH1121-760X2038-8306PAGEPress Publications, Pavia, Italy 10.4081/ejh.2018.2956Original PaperInteraction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study Sferra Roberta 1§Pompili Simona 1§Ventura Luca 2Dubuquoy Caroline 3Speca Silvia 4Gaudio Eugenio 5Latella Giovanni 6§Vetuschi Antonella 1§1 Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, Italy2 Pathology Division, San Salvatore Hospital, L’Aquila, Italy3 Intestinal Biotech Development, Lille, France4 U995-LIRIC-Lille Inflammation Research International Center, University of Lille, France5 Department of Anatomical, Histological, Forensic Medicine and Orthopedic Sciences, Sapienza University of Rome, Italy6 Department of Life, Health and Environmental Sciences, Gastroenterology Unit, University of L’Aquila, Italy§ These authors contributed equally to this workDepartment of Biotechnological and Applied Clinical Sciences, Human Anatomy, University of L’Aquila, Via Vetoio, Coppito 2, 67100, L’Aquila, Italy. +39.0862.433574, 433504 - +39.0862.433523. roberta.sferra@univaq.itContributions: RS, study design and manuscript drafting; SP, immunohistochemical analyses and participation in the study design; LV, immunohistochemical analyses; SS, CD, experimental DSS-induced colitis and assessment of macroscopic colorectal lesions; EG, study supervision; GL, AV, study coordination, supervision and critical manuscript revision. Conflict of interest: The Authors declare no conflict of interest. 31 7 2018 24 7 2018 62 3 295618 6 2018 19 7 2018 ©Copyright R. Sferra et al., 20182018Licensee PAGEPress, ItalyThis article is distributed under the terms of the Creative Commons Attribution Noncommercial License (by-nc 4.0) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.A concomitant action of multiple profibrotic mediators appears crucial in the development and progression of fibrosis. Sphingosine kinase/sphingosine 1 phosphate and transforming growth factor- β/Smads pathways are both involved in pathogenesis of fibrosis in several organs by controlling differentiation of fibroblasts to myofibroblasts and the epithelial-to-mesenchymal transition. However, their direct involvement in chronic colitis-associated fibrosis it is not yet known. In this study we evaluated the immunohistochemical expression of some proteins implicated in sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in Dextrane Sodium Sulphate (DSS)- induced colorectal fibrosis in mice. Compared to control mice, DSS-induced chronic colitis mice developed a marked intestinal fibrosis associated with a concomitant overexpression of TGF-β, p-Smad3, α-SMA, collagen I-III, SPHK1, RhoA, PI3K, Akt, p-Akt, p-mTOR. This study highlights the relationship between the two pathways and the possible role of SPHK1 in the intestinal fibrosis. These results, if confirmed by in vitro studies, may have important clinical implications in the development of new therapeutical approaches in inflammatory bowel disease. Keywords Intestinal fibrosisepithelial tomesenchymal transitionimmunohistochemistryTGFβ/Smadssphingosine 1-phosphate ==== Body Introduction Intestinal fibrosis is described as an uncontrolled accumulation of Extracellular Matrix (ECM) proteins and represents a common and progressive response to chronic inflammation, characterized by the rearrangement of normal tissue architecture, leading eventually to strictures, stenosis and intestinal obstruction, particularly in Crohn Disease (CD). Crucial effector cells of this process are activated myofibroblasts, derived not only from resident mesenchymal cells (fibroblasts, sub-epithelial myofibroblasts and smooth muscle cells) but also from epithelial and endothelial cells via a process of Epithelial-Mesenchymal Transition (EMT)/Endothelial-Mesenchymal Transition (EndoMT).1-3 Several lines of evidence suggest that EMT is a crucial mechanism in the fibro-proliferative processes.4 Several biological mediators have been implicated in the activation of ECM producing cells, including cytokines, chemokines, growth factors, angiogenic factors and products of oxidative stress. Among these, Transforming Growth Factor- β (TGF-β) plays a central role in sustaining and amplifying tissue remodeling and fibrosis. Previous studies have demonstrated that several anti-fibrotic molecules directly interact with the TGF-β/Smad pathway to modulate intestinal fibrosis.5,6 Although TGF-β signaling is dependent on Smad family protein activity, other molecules, including those that regulate cytoskeletal function, have been implicated in fibrosis. Members of the Rho-GTPase family, in particular RhoA, play a fundamental role in TGF-β-induced α-Smooth Muscle Actin (α- SMA) expression and EMT.7,8 TGF-β also activates the Phosphoinositide 3-Kinase (PI3K)/protein kinase B (AKT)/mammalian Target of Rapamycin (m-TOR)-dependent pathway, deregulated of which contributes to chronic fibrosis in several fibro-proliferative diseases.9 In addition, recent reports also demonstrate a connection between the TGF- β/Smad and sphingosine 1 Phosphate (S1P) pathways in stimulating excessive ECM production in many organs.10 S1P is a ceramide-derived lipid messenger of the sphingolipid family, the synthesis and stabilization of which involves structural membrane components. Sphingosines are bioactive cytosolic lipids, deregulation in the synthesis and degradation of which is toxic and characterized by membrane damage or modification.11 Under normal conditions, cells control cytosolic sphingosine accumulation through the balanced action of S1P-lyase (SPL)- mediated degradation and specific S1P phosphorylate.11 SPL is a crucial regulator of intracellular S1P levels and S1P phosphorylation is catalyzed by Sphingosine Kinase 1 (SPHK1) and Sphingosine Kinase 2 (SPHK2) that exhibit ubiquitous expression but differences in subcellular localization and biochemical properties.12 S1P exhibits both intracellular and extracellular functions: it acts as a second messenger, interfering with intracellular pathways involved in variety of cellular processes but also binds specific S1P1, S1P2, S1P3, S1P4, S1P5 cell surface receptors, involved in cell growth, differentiation, survival, motility, inflammation and fibrosis,12,13 each inducing a specific cellular response to S1P binding.14 There appear to be multiple ways through which crosstalk between S1P and SPHK1 and TGF-β signaling results in organ fibrosis.15 In idiopathic pulmonary fibrosis (IPF), S1P directly stimulates airway epithelial cells by activating latent TGF-β through Rho kinase and αvβ6 integrin. 16 TGF-β activates SPHK1, Akt and AMPK, resulting S1P production.17 Interaction between S1P/SPHK1 and TGFβ-1 signaling pathways induces the transition of alveolar epithelial cells into myofibroblasts resulting in the expression of fibronectin and α-SMA and progression of lung fibrosis.18 A link between S1P and TGF-β-associated fibrosis has also been demonstrated in the cardiac muscle, in which TGF-β stimulates SPHK1, inducing S1P production and activation of cardiac fibrosis via the S1P2 receptor.19 Furthermore, the paracrine/autocrine action of S1P, produced by SPHK1 in the intracellular compartment and released into the extracellular compartment, promotes TGFβ-induced cardiac fibroblasts to myofibroblasts trans-differentiation.20 Previous reports have also associated ECM accumulation and renal fibrosis with activation of the SPHK1/S1P and TGF-β pathways.21 Furthermore, diabetic kidney and mesangial cells grown under high glucose conditions exhibit augmented expression of the S1P2 receptor, implicating the S1P-S1P2-MAPK axis in fibronectin overexpression.13 S1P receptors are also expressed by liver and stimulate hepatic stellate cells proliferation and contractility in vitro.22 A potential role for S1P and S1P2 receptors in liver fibrosis and regeneration following carbon tetrachloride (CCl4) hepatic injury has also been reported in S1P2 knockout mice, characterized by reduced hepatic myofibroblasts numbers and proliferation in response to S1P. 22 We hypothesized that the TGF-β/Smad3 pathway, SPHK1, RhoA, PI3K, Akt, p-Akt, and p-mTOR may act as a complex signaling network, with extensive crosstalk and strong effects on intestinal fibrosis. Therefore, in this study, we evaluated the in vivo expression of all these molecules in a murine model of Dextran Sulphate Sodium (DSS)-induced colorectal fibrosis, and in particular the interaction between SPHK1 and TGF-β/Smad pathways. Materials and Methods The experimental colitis was carried out at the Institution of Pasteur, Animal Care Facility (Pasteur Institute de Lille, France) as per the governmental guidelines and approved by the Ethic Committee for Animal Experimentation of the region Nord-Pas de Calais-France (Comite d’Ethique en Experimentation Animale Nord-Pas de Calais; CEEA n.75). Animals Twenty C57BL/6 wild-type mice were included in the study (Janvier, Le Genest- St-Isle, France). All animals were kept in a pathogen-free environment at constant room temperature, with 12h/12h light/darkness cycle and food and water ad libitum. Experimental design, induction and evaluation of chronic colitis The mice were randomly divided into two groups: control (H2O) n=5 and DSS n=15. Chronic colitis and fibrosis were induced in mice by oral administration of 2.5% (w/v) DSS (MW: 36,000-44,000, purchased from TdB Consultancy, Uppsala, Sweden) solubilized in autoclaved tap water and administered ad libitum for three cycles (5 days DSS followed by 7 days of tap water) while control groups received only tap water. Animals were monitored daily for fluid intake, weight changes, and examined for signs of colitis such as weight loss, diarrhea (scored on a 0-2 scale, as follows: 0, absence; 1, mild; 2, severe), rectal bleeding, assessed with the ColoScreen III Lab Pack (Elitech, Salon-de-Provence, France) and rectal prolapse, (scored as 0, absence; 1, presence). Samples recovery and preparation Four days after the last DSS cycle administration, the animals of each group were sacrificed through cervical dislocation after CO2 anaesthesia. Following laparotomy, colon and rectum were identified and rapidly excised. On a scale from 0 to 2, the presence of adhesions between the colonrectum and adjacent organs was assessed.23 The length and the weight of colon and rectum were measured and then scored for macroscopic lesions. Then the colorectal samples were fixed in 4% buffered formaldehyde and embedded in paraffin for histological and immunohistochemical analysis. Macroscopic and microscopic evaluation of colonic injury In order to score the macroscopic lesions, the colonic length and weight were measured. Macroscopic colonic lesions were scored on a 0-2 scale, as follows: colonic adhesions (0, absence; 1, mild/focal zonal; 2, severe/diffuse); colonic dilation (0, absence; 1, mild; 2, severe); colonic thickness (0, normal; 1, mild increase; 2, marked increase, >3 mm).23 The total macroscopic score was expressed as the sum of the individual scores of colonic alterations. Bowel fragments were subjected to conventional histological processing procedures, fixation in 4% buffered formalin in Phosphate Buffer Saline (PBS) (pH 7.4 for 3 h at room temperature) and paraffin embedding. Serial 3-μm sections were staining using Haematoxylin and Eosin (H&E), in order to highlight the degree of inflammation, and Masson’s Trichrome to evaluate the deposition of connective tissue and fibrosis. The stained sections were then observed under an Olympus BX51 Light Microscope (Olympus Optical Co. Ltd., Tokyo, Japan). Two pathologists (AV and RS) independently examined and scored all histological sections of the colonic samples in double-blind, according to the presence of ulcerations (0, absent; 1, small ulcers; 2, big ulcers), degree of inflammation (0, absent; 1, mild; 2, moderate; ad 3, severe), depth of the lesions (0, absent; 1, lesions extending in the submucosa; 2, lesions in the muscolaris propria; 3, lesions in the serosa) and degree of fibrosis (0, absent; 1, mild; 2, moderate; 3, severe). The sum of these scores was expressed as total microscopic score as previously reported.23 Intestinal inflammation was evaluated as absent, mild, moderate or severe according to the density and the extent of the inflammatory infiltrate, the loss of goblet cells, and the bowel wall thickening. Masson’s Trichrome allowed to stage intestinal fibrosis as mild, moderate or severe, based on the density and the extent of connective tissue and disruption of tissue architecture.23 Immunohistochemistry Colonic samples, were also promptly fixed in 4% buffered formalin in PBS for 3 h at room temperature, dehydrated in graded ethanols and embedded in low-melting paraffin. Sections of 3-μm in thickness were incubated for 40 min in methanol and in 3% hydrogen peroxide solution for 5 min. The specimens were incubated overnight at 4°C with specific antibodies (Table 1). The samples were washed in PBS for 5 min and incubated with streptavidinbiotin- peroxidase conjugated secondary antibody (K0675, Dako-Cytomation, Milano, Italy). After rinsed in PBS for 10 min the sections were incubated with 3,3- diaminobenzidine-tetrahydrochloride for 1-3 min. Finally, the sections were counterstained with Mayer’s Hematoxylin, mounted and observed under the Olympus BX51 Light Microscope (Olympus, Optical Co. Ltd., Tokyo, Japan). To control the specificity of the immune reaction, negative and positive controls were performed for all immunoreactions (Table 1). For negative controls the sections were incubated omitting the primary antibody. Quantitative digital image analysis of immunohistochemical staining and statistical analyses Quantitative comparison of immunohistochemical staining was measured by ImageJ, a digital image analysis public domain software (U.S. National Institutes of Health, Bethesda, MD; available at: https://imagej.nih.gov/ij/) for all antibody tested in the study. Five microscopic fields were selected from control and DSS groups, photographed at the same magnification and then subjected to IHC profiler software analysis. The immunopositivity was expressed as a percentage of the total softwareclassified areas and the data obtained were plotted as histograms. Results were expressed as means ±SD; a P-value <0.05 was considered statistically significant. Results Clinical and macroscopic findings of chronic colitis induced by DSS Administration of DSS has led to a moderate body-weight loss and mortality in 13% of the animals. The colon of DSS treated mice appeared increased in weight (referred to the last 8 cm of the distal large bowel) whereas colon length was reduced compared to control mice (Figure 1). In DSS group the ratio weight/length of the colon and the major macroscopic findings of fibrosis as dilation, thickness, stenosis and adhesion were significantly increased compared to untreated mice (Table 2). Microscopic features of colitis In DSS treated mice histological evaluation assessed by H&E and Masson’s Trichrome staining showed marked signs of inflammation and evidence of fibrosis (Figure 2 B,D), compared to controls (Figure 2 A,C). Mucosal and submucosal layers displayed inflammatory infiltrate and a reduction of goblet cells, associated with a marked distortion of crypt architecture and presence of erosions and ulcerations. Furthermore, a significant increase in connective tissue deposition was found in submucosa and serosa. Immunohistochemistry The main fibrotic markers Collagen IIII and α-SMA resulted mild expressed in control mice (Figure 3 A,D), while there was a significant increase in immunopositivity in DSS mice (Figure 3 B,E) as confirmed by quantitative data (Figure 3 C,F). In particular, in DSS treated group, α-SMA staining was not limited to the smooth muscle cells of the muscularis mucosa and muscularis propria but it was also present in lamina propria, submucosa and serosa (Figure 3E). In control group the expression was restricted to typical layers (Figure 3D). Immunopositivity for TGF-β and phosphorylated Smad3 resulted slightly expressed in untreated control mice (Figure 4 A,D), while they were markedly increased in DSS group (Figure 4 B,E). Concerning Smad7 inhibitor protein, the immunopositivity was significantly higher in control respect to DSS (Figure 4 G,H). These data were confirmed by quantitative analysis (Figure 4 C,F,I). Immunohistochemical and quantitative analysis for Sphingosine Kinase 1 (SPHK1) showed an increased expression in DSS mice (Figure 5 B,C) compared to control group (Figure 5 A,C), according to the fact that TGF-β is able to activate and upregulate this molecule in fibrotic condition. Above all, SPHK1 positivity was predominantly localized in mucosa layer and in submucosa of colon from DSS mice. RhoA and PI3K staining resulted slightly expressed in control mice (Figure 5 D,G), while was highlightly increase in DSS treated group, mainly in mucosa (strongly in the ephitelium, Supplementary Figure 1), but also in submucosa and in serosa (Figure 5 E,H). These data were confirmed by quantitative analysis (Figure 5 F,I). A marked increase of both Akt and phosphorilated Akt was observed in mucosa, submucosa and serosa of DSS treated mice (Figure 6 B,E) compared to untreated mice (Figure 6 A,D) has also shown by quantitative assessment (Figure 6 C,F). Phosphorilated m-TOR staining was mildly expressed in mucosa of control mice (Figure 6G), while was increased in mucosa (specially in the epithelial cells, Supplementary Figure 1), in submucosa and in serosa of mice receiving DSS (Figure 6H). The different levels of immunopositivity between the two groups were also highlighted by quantitative analysis (Figure 6I). Discussion The activation of SPHK1 and generation of S1P, the bioactive lipid of sphingomielin, plays an important role in a variety of physiologic and pathologic cellular processes in many organs.9,13,17 Several evidences suggest a pro-fibrogenic role of SPHK1 pathway carried out through different mechanisms including the influence on epithelial barrier function, the inflammarory cell recruitment and the activation of myofibroblast (migration, proliferation, differentiation and contraction). In the present study we investigate the in vivo interaction between TGF-β and SPHK1 signaling in a mouse model of DSS-induced chronic colitis, in order to verify whether SPHK1 and its effectors were able to interact with TGF-β/Smad signaling and if they were involved in the induction and progression of the intestinal fibrosis. Clinical signs observed in DSSreceiving mice were coherent with the development of colonic fibrosis, with a significant increase of the total macroscopic score and histological parameters, compared to control mice. Immuno - histochemical evaluations demonstrated overexpression of the main marker of myofibroblasts activation, with consequent accumulation of collagen in lamina propria and submucosal layer altering the normal architecture of colonic wall with respect to the normal morphology of the control group. According to other research group, which have previously shown the potential profibrotic role of SPHK1 in pulmonary, cardiac, liver and kidney fibrosis, we demonstrate a close relationship between TGF-β and SPHK1 expressions in mice developing intestinal fibrosis. A concomitant upregulation of TGF-β-1, p-Smad3, SPHK1, RhoA, PI3K was found in DSS mice compared to controls. These findings demonstrated that SPHK1 and RhoA are mediators of inflammation and fibrosis. As it occurs in other organs,24,25 the increasing expression of SPHK1 could influence the upregulation of RhoA responsible for inflammatory gene expression subsequently leading to activation of autocrine inflammatory intestinal signaling. We have also observed an increased expression of PI3K, AKT, p-AKT and p-m-TOR in all colonic layers of DSS treated mice compared to controls. TGF-β can activate PI3K/AKT/m- TOR signaling and RhoA may be an upstream mediator of AKT activation that can be also involved in the EMT. 26,27 SPHK1 appears to facilitate also in the intestine the action of the critical pro-fibrotic factor TGF-β as occur in other organs, promoting transformation of fibroblasts and epithelial cells into activated myofibroblasts. 28 These in vivo data support the hypothesis that TGF-β stimulates production of S1P via increased SPHK1 activity which is then released and acts on the S1P2 receptor to promote pro-fibrotic cellular transformation and function. Therefore, the development of intestinal fibrosis could be influenced not only by TGF-β/Smad pathway but also by crosstalk between TGF-β/SPHK1/S1P signaling by influencing the expression of profibrotic gene as collagen and α-SMA levels (Figure 7). Despite more detailed investigations, such as in vitro studies, are needed to elucidate the involvement of the different S1P receptors in chronic intestinal inflammation and fibrosis, these preliminary data support the hypothesis of a possible interaction between the above-mentioned molecules in the development of colorectal fibrosis in mice. Development of molecules to regulate the synthesis of S1P and to selectively block or stimulate different S1P receptor subtypes could be an attractive target for the development of new pharmacological treatments of intestinal fibrosis.29 Figure 1. Macroscopic aspects of large bowel in DSS and control mice. Figure 2. H&E and Masson Trichrome staining (original magnification 10x). DSS treated mice (B,D) show marked signs of inflammation (B) and several evidences of fibrosis (D) while in control specimens (A,C) a normal architecture of colonic wall is present. Figure 3. Immunohistochemistry (original magnification 10x). Collagen I-III and α-SMA are mild expressed in control mice (A,D), while there is a significant increase in immunopositivity in DSS mice (B,E). Figure 4. Immunohistochemistry (original magnification 10x). TGF-β, p-Smad3, are significantly increased in DSS mice (B,E) while they are mild expressed in control mice (A,D). The inhibitor protein Smad7 shows a lower positivity in DSS mice (H) respect to control (G). Figure 5. Immunohistochemistry (original magnification 10x). The immunopositivity of SPHK1, RhoA and PI3K is prevalent in DSS mice (B,E,H) respect to control (A,D,G). In particular, in DSS mice, SPHK1 expression is predominantly localized in mucosa layer and in submucosa of colon (B). In the same group RhoA and PI3K staining results highly expressed (E,H) in mucosa, especially in the ephitelium (supplementary Figure 1), but also in submucosa and in serosa. Figure 6. Immunohistochemistry (original magnification 10x). A marked increase of both Akt and phosphorilated Akt is observed in mucosa, submucosa and serosa of DSS treated mice (B,E) compared to controls (A,D). Phosphorilated m-TOR staining is mild expressed in mucosa of control mice (G), while the immunopositivity is increased in epithelial cells (supplementary Figure 1), in submucosa and in serosa of DSS treated mice (H). Figure 7. Schematic representation of TGF-β dependent signaling implicated in fibrosis process. TGF-β upon biding its receptors, activates both canonical Smads pathway and noncanonical pathway (SPHK1/S1P/mTOR), inducing transcription of pro-fibrotic target genes. Table 1. Antibodies used with their sources and dilutions together with the positive controls. Antibodies Sources Dilutions Positive controls TGF-β (Sc-146) Santa Cruz Biotecnology Inc., Santa Cruz, CA, USA 1:400 Intrinsic in the sample p-Smad3 (Sc-130218) Santa Cruz Biotecnology Inc., CA, USA 1:100 Intrinsic in the sample Smad7 (Sc-365846) Santa Cruz Biotecnology Inc., Santa Cruz, CA, USA 1:400 Intrinsic in the sample α-SMA (Sc-32251) Santa Cruz Biotecnology Inc., Santa Cruz, CA, USA 1:400 Vessel wall Collagen I-III (Sc-8784; sc-8781) Santa Cruz Biotecnology Inc., Santa Cruz, CA, USA 1:400 Intrinsic in the sample SPHK1 (PA1996) BosterBio, Pleasanton, CA, USA 1:100 Human kidney cancer RhoA (SA2326231) ThermoFisher Scientific, Milan, Italy 1:100 Human kidney cancer PI3K (Sc- 2355932) ThermoFisher Scientific, Milan, Italy 1:100 Human prostate cancer Akt (ENTO-185) ElabScience Biotecnology Co., Houston, TX, USA 1:100 Human prostate cancer p-Akt (44621-G) ThermoFisher Scientific, Milan, Italy 1:100 Human prostate cancer p-mTOR (F17-65R) SignalChem, Richmond, BC, Canada 1:100 Human prostate cancer Table 2. Clinical findings and macroscopic features of DSS-induced chronic colitis. Parametres H2O DSS Duration of DSS administration (days) 0 36 Number of mice 5 15 Mortality (n.mice) 0 2 Body weight (gr) 28.44±0.51 26.20±0.31** Colon weight (gr) 0.187±0.006 0.305±0.008** Colon lenght (cm) 8.78±0.015 6.8±0.19** Colon weight/size ratio (fold change) 1±0.042 2.113±0.08** Dilatation np 1.23±0.22** Tickness np 0.76±0.2* Stenosis np 0.92±0.23* Adhesions np 0.73±0.22 np Total macroscopic score np 3.64±0.435** Data are expressed as mean±SEM; np, not present *P<0.05 vs H2O **P<0.01 vs H2O. ==== Refs References 1. Fiocchi C Lund PK Themes in fibrosis and gastrointestinal inflammation . Am J Physiol Gastrointest Liver Physiol 2011 ;300 :G677 -83 .21415411 2. Rieder F Fiocchi C. Mechanisms of tissue remodeling in inflammatory bowel disease . Dig Dis 2013 31 :186 -93 .24030223 3. Lawrance IC Rogler G Bamias G Breynaert C Florholmen J Pellino G Cellular and molecular mediators of intestinal fibrosis . 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