==== Front Ann Saudi MedAnn Saudi MedAnnals of Saudi Medicine0256-49470975-4466King Faisal Specialist Hospital and Research Centre 10.5144/0256-4947.2008.59asm-1-59LettersThe use of cercariae infection of the Bulinus truncatus snail for evaluation of schistosomiasis control in Iran Farahnak Ali Mobedi Iraj Eshraghian Mohammad Reza Correspondence address: Associate Professor Department of Parasitology and Mycology, School of Public Health, Medical Sciences University of Tehran, Tehran, Iran, T: 98 21 8951583, F: 98 21 6462267, PO Box:14155-6446, Tehran, Iran, farahnak@sina.tums.ac.irJan-Feb 2008 28 1 59 59 Copyright © 2008, Annals of Saudi Medicine2008This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. ==== Body To the Editor: Urinary schistosomiasis caused by Schistosoma haematobium has been reported from many countries in Africa and Asia.1 Many studies have been conducted on the distribution, ecology, biology and host-parasite relationships of the snail in Khuzestan during the 1970s by the staff of the Schistosomiasis Control Project.2–5 Because of political changes in the neighboring country of Iraq where urinary schistosomiasis was prevalent, and because of the constant travel of Iraqi citizens to these areas, local and national health authorities wanted an evaluation of the current situation of the disease from the viewpoint of parasite transmission from snails to the local inhabitants.6 Therefore, the present study was conducted to determine the presence of Schistosoma cercariae in local Bulinus snails as criteria for evaluation of the schistosomiasis control program in the rural area of the southwest of Iran. A total of 2400 B. truncatus snails were randomly collected from southern and eastern districts of the Dezful region and transferred to a health research centre. Collected snails were examined for shedding cercariae by using artificial light, leaving over night or by crushing snails in a glass plate. Cercariae were identified by systematic key references.7 Of the total of Bulinus snails examined for schistosome cercariae, 52 (2%) were found to be infected with Amphistome cercariae, and 15 (0.05%) with Strigea cercariae (Figures 1, 2, 3). Not a single Schistosoma cercaria was obtained from the snails. Evaluation of the progress of schistosomiasis control programs, by case detection, when the infection rate becomes extremely low, is expensive, time consuming and difficult. For these reasons, the method of examination of the snail intermediate host for the presence of cercariae of the parasite can be used as a new approach for evaluation of the transmission status of the infection. The abundance of snail hosts in this area, as well as the visiting of Iraqi citizens who may come from schistosomiasis-infested areas of Iraq, may cause reinfection of the local snails with the parasite. Various cercariae species including Strigea cercariae and Amphistome cercariae obtained from B. truncatus by shedding or crushing methods, can be easily distinguished from cercariae of schistosomes, which has no pharynx. During the summer, Khuzestan province is very hot. For this reason, people, especially children, swim and play in the rivers or canals, which are used for agricultural purposes. In addition, many young children work on the agricultural farms without any protection on their hands or feet where S. haematobium as well as animal schistosome cercariae can readily penetrate the skin.8 Lack of S. haematobium cercariae in our samples in this region revealed that snails are free from this parasite and support the theory that transmission of schistosomes cecaria does not occur in the districts and therefore this disease is still under control, which is good news for inhabitants of the region as well as for local health authorities. Because of the presence of Bulinus in the other parts of province, use of this protocol for evaluating the transmission status of S. haematobium by finding cercariae of the parasite among local B. truncatus is recommended. This study was supported by a grant (240/802) from the School of Public Health and the Institute of Public Health Research at Medical Sciences/ University of Tehran. Special thanks to Professor Freidon Arfaa for review and his comments on the article. The authors would like to thank the Mr. R. Pourshojaei and Mr. M. Jahanbani for collection of snails. Thanks also Miss M. Rohnavaz for maintaining of snails in the lab. ==== Refs REFERENCES 1 WHO Expert Committee Prevention and control of schistosomiasis and soil-transmitted helminthiasis World Health Organ Tech Rep Ser 2002 912 1 57 2 Chu KY Massoud J Arfaa F Distribution and ecology of Bulinus truncatus in Khuzestan, Iran Bull World Health Organ 1968 39 607 37 5304436 3 Chu KY Massoud J Sabbaghian H Host-parasite relationship of Bulinus truncatus and Schistosoma haematobium in Iran. 4. Effect of month of infection on cercarial-incubation periods of S. haematobium and S. bovis Bull World Health Organ 1966 34 135 40 5295560 4 Chu KY Arfaa F Massoud J The survival of Bulinus truncatus buried in mud under experimental outdoor conditions Ann Trop Med Parasitol 1967 61 6 10 6069306 5 Massoud J Arfaa F Farahmandian I Ardalan A Mansoorian A Progress in the national schistosomiasis control programme of Iran Bull World Health Organ 1982 60 577 82 6982780 6 Youssef AR Cannon JM Al Juburi AZ Cockett AT Schistosomiasis in Saudi Arabia, Egypt, and Iraq Urology 1998 51 5A Suppl 170 4 9610576 7 Schell SC The trematodes WmC Brown Company Publishers 1970 18 34 8 Farahnak A Essalat M A study on cercarial dermatitis in Khuzestan province, south western Iran BMC Public Health 2003 3 www.biomedcentral.com/1471-2458/3/35