==== Front Ann Saudi MedAnn Saudi MedAnnals of Saudi Medicine0256-49470975-4466King Faisal Specialist Hospital and Research Centre 1676144710.5144/0256-4947.2006.110asm-2-110Original ArticleCorrelation of leptin and sex hormones with endocrine changes in healthy Saudi women of different body weights Al-Harithy Rowyda N. Al-Doghaither Huda Abualnaja Khalid From the Department of Biochemistry, King Abdulaziz University, Jeddah, Saudi ArabiaCorrespondence and reprint requests: Rowyda N. Al-Harithy, PhD, Biochemistry Department, King Abdul Aziz University, Jeddah, P.O. Box 40288, Jeddah 21499, Kingdom of Saudi Arabia. Mobile: +966-505 444238, Fax: +966-2-639 3640, ralharithy@yahoo.comMar-Apr 2006 26 2 110 115 01 12 2005 Copyright © 2006, Annals of Saudi Medicine2006This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.BACKGROUND A relationship between estrogen and leptin has been described during the follicular phase of both spontaneous menstrual cycles and cycles stimulated with exogenous follicle-stimulating hormone (FSH), which suggest that leptin has either a direct effect on or is regulated by gonadal steroids in the human ovary. To examine the changes in plasma leptin levels during the menstrual cycle, we studied the association between plasma leptin and reproductive hormones in young, healthy Saudi women. SUBJECTS AND METHODS Sixty-five young women between 19 to 39 years of age, with a normal menstrual cycle, were grouped into 33 overweight and obese females of BMI >25 kg/m2, and 32 lean females of BMI <25 kg/m2. Anthropometrics measurements were made at the time of the collection. Samples were analyzed for leptin, progesterone, estradiol (E2), FSH, luteinizing hormone (LH), cortisol, and testosterone concentrations. RESULTS Overweight and obese women, compared with lean, tended to have a significantly higher plasma leptin levels (11.38± 4.06 vs. 6.22± 2.87 ng/mL; P=0.05). In overweight and obese subjects, circulating leptin concentrations showed a direct correlation with BMI (r=0.53; P=0.002), hip circumference (r=0.32; P=0.005), waist-hip ratio (r=0.37; P=0.042), weight (r=0.41; P=0.021), and E2 on day 3 (r=0.35; P=0.048). In all correlation analyses, leptin levels did not correlate with cortisol or testosterone. In lean subjects, a bivariate correlation analysis showed that plasma leptin concentrations were directly correlated to hip circumference (r=0.43; P=0.012). Moreover, a direct correlation was found with progesterone on day 10 (r=0.43; P=0.014) and E2 on day 24 (r=0.47; P=0.007). CONCLUSION There is a link between plasma leptin and progesterone concentrations during the menstrual cycle, and the variation in circulating estradiol concentrations may have an influence on circulating leptin in female subjects. ==== Body Leptin is a multifunctional adipostatic hormone that is produced by the obese (ob) gene.1 Although white fat cells are its main producers, it is now known that it is made by several other kinds of cells as well.2 Leptin plays a key role in regulation of food intake and energy expenditure.3 Studies in humans and animals have shown that leptin concentrations in the blood correlate with the amount of adipose tissue in the body, acting as a sensing hormone in a negative feedback control from adipose tissue to the hypothalamus.4 There is considerable interindividual variation in plasma leptin concentrations among individuals with a comparable degree of obesity, suggesting that other factors are involved in the regulation of leptin production. Furthermore, human subjects with leptin gene mutations are markedly obese5 and lose weight in response to exogenous administration of leptin.6 However, human obesity is not usually associated with leptin deficiency, but largely with leptin resistance.7 Recent investigations have shown that leptin receptors are widely distributed in a variety of tissues, and that any defects in the genes encoding leptin receptors lead to a spectrum of manifestations, including diabetes.8,9 In humans, there is also evidence that leptin is involved in reproduction function, including puberty, and with the maintenance of normal menstrual cycles.10 A number of studies have proposed that if leptin is the signal that there are adequate fat stores to start and maintain ovulation and menstruation, it may account for these changes through its effects on the ovary11–13 or on the brain.14 Another study suggested that, at the level of the central nervous system, leptin may stimulate gonadotropin-releasing hormone (GnRH) release from the hypothalamus, and luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release from the pituitary, probably by acting on its own receptor and promoting nitric oxide release.15 Studies have found that high leptin levels during the luteal phase of spontaneous menstrual cycles are correlated with LH levels.16–19 Also, a relationship between estrogen and leptin has been described during the follicular phase of both spontaneous cycles and cycles stimulated with exogenous FSH.20 This suggests that leptin either has a direct effect or is regulated by gonadal steroids in the human ovary. Given this background, we sought to examine the changes in plasma leptin levels during the menstrual cycle and study the association between plasma leptin, body fat mass (using body mass index, BMI), fat distribution (waist circumference, hip circumference, waist-hip ratio, WHR), and reproductive hormones in young, healthy Saudi women. Subjects and Methods Experiments were carried out in 65 healthy female volunteers, aged 19–39 years, who had regular menstrual cycles between 27 and 30 days in length (Table 1). Their ovulatory cycles were assessed according to the information recorded about their last eight menstrual cycles. None had received drugs known to interfere with hormonal concentrations for at least 3 months before the study, and women with polycystic ovary syndrome (PCOS) were excluded. All subjects in the study provided informed consent for all procedures. Blood samples were collected after an overnight fast, on day 3, 10, 17, and 24 from the beginning of the menstruation from all the participants. All blood samples were taken between 08:00 and 10:00 AM after an overnight fast. At each time of collection, information was recorded for all subjects, including weight, height, waist, and hip circumference. BMI was calculated by dividing the body weight (in kilograms) by the square of the height (in meters). The waist circumference was taken as the largest standing horizontal circumference between the ribs and the iliac crest; the hip circumference was taken as the largest horizontal circumference of the buttocks. WHR was calculated by dividing the waist by the hip circumference. The concentration of leptin in plasma was determined by a solid-phase sandwich ELISA with an affinity-purified polyvalent antibody immobilized in microtiter wells using a commercial Direct ELISA human leptin kit according to the procedure provided by the company (Diagnostics Biochem, Canada). Assays were performed in duplicate. The concentration of cortisol was determined in duplicate using a commercial DRG ELISA kit (DRG Diagnostics, Germany). Serum progesterone, estradiol, FSH, LH, and testosterone were measured by electrochemiluminescence ECLIA methods (Roche Diagnostics, Mannheim, Germany) on the Roche Elecsys 2010 Immunoassay Analyzer (Tokyo, Japan). Statistical analysis was performed using SPSS 10 for Windows. One-way ANOVA, paired-sample and independent-sample t tests were applied where appropriate. Correlations were studied by Pearson’s method. Multivariate analysis was performed using a multiple linear regression model. All comparisons or regression parameters were considered statistically significant at a P level less than 0.05. Results Compared with lean women, overweight and obese women tended to have a significantly higher mean BMI, WHR, body weight, waist, serum cortisol, and plasma leptin levels (Table 1). There were no significant differences in hip circumference, height, and testosterone between the two groups. Overweight and obese individuals had a serum leptin level between 7.05 and 21.73 ng/mL and lean women had a serum leptin between 2.04 and 15.61 ng/mL. There was a physiological fluctuation in leptin concentrations during the menstrual cycle, with the lowest values during the early follicular phase and the highest during the luteal phase in the two groups (Table 2). In the overweight and obese groups, the mean plasma leptin level on day 3 of the menstrual cycle was 10.58± 5.47 ng/mL, which varied significantly with day 10 (P= 0.02), 17 (P= 0.009) and 24 (P=0.001). In the same group, estradiol, FSH, and LH fluctuated with a significant increase (P= 0.0001) during the ovulating phase (day 17) with respect to day 3. Leptin concentration peaks during the luteal phase (day 24) were coincident with maximal progesterone levels on day 24. Progesterone showed significant variations on day 17 (P=0.052) and day 24 (P=0.0001) with respect to day 3. To provide an overview of the relationships between leptin, body composition and hormones, a bivariate correlation was performed. Table 3 shows bivariate and partial correlations between leptin levels and specific variables in both groups. In the partial analyses, successive adjustments were made for BMI and hip circumference in separate analyses. In overweight and obese subjects, circulating leptin concentrations showed a direct correlation with BMI (r=0.53; P=0.002), hip (r=0.32; P=0.005), WHR (r=0.37; P=0.042), weight (r=0.41; P=0.021), and E2 on day 3 (r=0.35; P=0.048). In all correlation analysis, leptin levels did not correlate with cortisol or testosterone. In lean subjects, bivariate correlation analysis showed that plasma leptin concentrations were directly correlated to hip circumference (r=0.43; P=0.012). Moreover, a direct correlation was found with progesterone on day 10 (r=0.43; P=0.014) and E2 on day 24 (r=0.47; P=0.007). Progesterone and E2 did not differ significantly in the bivariate analysis after adjustment for BMI and hip circumferences. A correlation appeared between leptin and progesterone on day 24 after adjusting for hip circumferences (r=0.36; P=0.048). The finding from the above bivariate correlation analysis were further explored using stepwise multiple linear regression analysis with leptin concentration as the dependent variable. Factors that correlated with leptin in the bivariate analysis were introduced into the model. Hip circumferences and E2 on day 24 remained significant predictors, explaining 61.1% of the variation in leptin levels in lean subjects. BMI and hip circumferences were significant predictors, explaining 68.4% of the variation in leptin levels in the other group. Discussion The data reported in this study are derived from a representative sample of the general population in Saudi Arabia. The volunteers were healthy women between the ages of 19 and 39 years. This study clearly shows a strong and highly significant positive association between plasma leptin concentrations and BMI as has been reported in numerous scientific studies.21–24 This relationship between plasma leptin levels and relative adiposity appears to be consistent across broad ranges of body size and composition represented by individuals from different countries, regardless of environmental differences. An interesting result in this study is that individuals with a BMI higher than 30 kg/m2 have almost three times the circulating level of leptin compared with those with a BMI less than 25 kg/m2. This result may indicate that at a certain BMI level, plasma leptin tends to increase rapidly; this may explain the complications related to obesity. Apart from the function of leptin in the central nervous system on the regulation of food intake and body energy balance, it may well be one of the hormonal factors that signal the body’s readiness for reproduction. It has been known that menstrual function is preserved after leptin reaches a threshold level.25 Anorexic women showed lower LH and leptin levels than menstruating women with low body mass indexes. Women athletes have also shown menstrual disorders and reproductive disruption when energy availability (dietary energy intake minus exercise energy expenditure) falls below a threshold between 20 and 30 kcal × kg LBM (−1) × d (−1).26 On the other hand, obese women often have menstrual irregularities that are corrected by a modest amount of weight loss.27 Thus, leptin seems to be an important determinant for reproduction, but the mechanisms mediating this effect are still obscure. Results from a recent study suggest that leptin replacement on pituitary hormone regulation in patients with severe lipodystrophy improved menstrual abnormalities, increased serum E2 level and decreased testosterone concentrations.28 These results add to the growing body of evidence that leptin plays a role in neuroendocrine regulation. In this study, the results of the physiological fluctuations in leptin concentrations during the menstrual cycle confirm previously published results.29–31 The lowest leptin values were detected during the early follicular phase and the highest during the luteal phase of the cycle, in both groups. The evaluation of the menstrual cycle showed a significant difference in leptin levels between the phases. This result is in agreement with previous studies20,32,33 and in disagreement with one other study.30 The observed variation in leptin levels between phases seems to be influenced by a factor or factors on leptin expression. Moreover, increased leptin concentrations in the luteal phase may help prepare the body for the metabolic demands of pregnancy. The variations in plasma leptin concentrations during the menstrual cycle do not appear to be related to cortisol and testosterone. In comparing the pattern of hormonal fluctuations throughout the menstrual cycle, leptin and progesterone show similar patterns of fluctuation, with a significant increase in serum concentrations on days 10, 17 and 24. This suggests a common stimulation factor for progesterone and leptin secretion. A previous study had suggested a stimulatory effect of progesterone on leptin secretion, which may be responsible for the fluctuations in leptin levels during the menstrual cycle.20 Mannucci et al.29 showed no correlation and suggested that progesterone has no effect on leptin secretion. The literature on the relationship between leptin and progesterone is inconsistent and the relationship appears to be complex. On the other hand, a stimulatory action of E2 appears to be most likely, as the increase in E2 concentrations precedes that of leptin. A previous study had suggested that the relation between leptin and E2 may depend on other factors.34 There are some limitations to this study. The design of this study did not permit us to confirm the stimulatory action of E2 and progesterone on leptin secretion. Further studies in vitro and in vivo are needed to explore these relationships. Second, there may be a selection bias of enrolled subjects due to the relatively small sample size in this study. In conclusion, the results suggest that there is a link between plasma leptin and progesterone concentrations during the menstrual cycle, and the variation in circulating estradiol concentrations may have an influence in circulating leptin in female subjects. The challenging question is: Does leptin need additional molecules and hormones to regulate energy metabolism and reproduction? Undoubtedly, the years to follow will increase our understanding of the relationship between leptin and sex hormones. Table 1 Characteristics of the study subjects. Variables Lean (n=33) Obese (n=32) P (t test) Body weight (kg) 53.70±6.64 79.87±11.72 0.003 Height (m) 1.60± 0.06 1.61±0.06 NS BMI (kg/m2) 20.94±2.14 30.57±3.90 0.004 Hip circumference (cm) 96.07±5.37 112.80±7.49 NS Waist (cm) 67.24±4.64 85.19±9.69 0.002 WHR 0.70± 0.03 0.76± 0.07 0.0001 Serum cortisol (ηmol/L) 357.86±210.8 331.68±145.0 0.023 Serum testosterone (ηmol/L) 0.61±0.35 0.56±0.41 NS Plasma leptin (ng/mL) 6.22±2.87 11.38±4.06 0.05 Data are mean±SD. Plasma and serum concentrations were measured during the fasting state. Body mass index (BMI). Waist-to-hip ratio (WHR). Non-significant (NS). Table 2 Values (mean ± S.D.) of follicle-stimulating hormone (FSH), luteinizing hormone (LH), estradiol, and progesterone during the menstrual cycle of obese and lean women. Hormones Days of the menstrual cycle Day 3 Day 10 Day 17 Day 24 FSH (mIU/mL)  Obese 5.99±1.47 5.02±1.85 4.34±2.18* 2.74±1.45**  Lean 7.19±2.93 5.79±3.07** 4.99±2.16** 4.35±2.62** LH (mIU/mL)  Obese 4.19±1.91 8.56±3.94** 15.86±14/53** 8.74±7.74*  Lean 5.88±2.68 9.79±11.73* 15.21±13.42** 9.34±9.05* Progesterone (ηmol/L)  Obese 4.52±7.61 1.21±1.33** 4.64±9.38 7.89±6.73**  Lean 3.15±5.25 2.37±1.01 5.56±6.13 9.83 ±7.72** Estradiol (ηmol/L)  Obese 222.63±174.56 487.75±421.74** 829.05±606.75** 739.06±448.06**  Lean 138.08±79.02 425.32±287.04** 857.11±571.33** 632.53±314.26** Leptin (ng/mL)  Obese 10.58±5.47 7.65±2.99* 9.60±4.09** 12.67±13.57**  Lean 6.70±4.27 8.07±5.83** 7.77±3.80** 10.01±5.18** All comparisons with day 3 values, two-tailed test; * P<0.05; ** P<0.01. Table 3 Bivariate and partial correlations of mean leptin concentration with selected variables. Variables Lean (n=33) Obese (n=32) Bivariate correlation (r) Partial correlations adjusted for BMI Partial correlations adjusted for hip Bivariate correlation (r) Partial correlations adjusted for BMI Partial correlations adjusted for hip Body mass index (BMI) 0.26 - −0.15 0.53* - 0.60 Weight 0.22 −0.01 −0.32 0.41* −0.13 0.31 Hip circumference 0.43* 0.39 - 0.32* −0.21 - Height −0.01 −0.02 −0.22 −0.06 −0.1 −0.23 Waist circumference 0.25 0.09 −0.11 0.50 0.11 0.41 Waist to hip ratio (WHR) −0.14 −0.19 −0.11 0.37* 0.18 0.39 Fasting serum cortisol 0.21 0.14 0.11 0.01 0.05 0.06 Fasting serum testosterone 0.16 0.64 −0.01 −0.03 −0.11 −0.04 Progesterone on day 3 0.01 0.02 0.04 0.06 −0.05 0.18 Progesterone on day 10 0.43* 0.42* 0.35* −0.07 0.21 −0.21 Progesterone on day17 −0.31 −0.31 −0.31 0.18 −0.11 −0.13 Progesterone on day 24 0.26 0.29 0.36* −0.39 −0.35 −0.5 Estradiol on day 3 −0.09 −0.07 0.01 0.35* −0.09 0.16 Estradiol on day 10 −0.31 −0.35 −0.35 0.12 −0.09 0.06 Estradiol on day17 0.27 0.22 0.24 −0.27 −0.08 −0.33 Estradiol on day 24 0.47** 0.49** 0.47** −0.17 −0.18 −0.36 FSH on day 3 −0.09 −0.15 −0.21 −0.35 −0.18 −0.21 FSH on day 10 −0.09 −0.11 −0.08 −0.01 0.2 −0.05 FSH on day 17 0.04 0.03 0.03 0.25 0.42 0.19 FSH on day 24 −0.12 −0.22 −0.23 0.43 0.49* 0.34 LH on day 3 −0.12 −0.07 −0.04 −0.4 0.043 −0.46 LH on day 10 −0.1 −0.09 −0.05 0.17 0.16 0.06 LH on day 17 0.23 0.21 0.19 −0.12 0.05 −0.25 LH on day 24 0.24 0.18 0.11 0.41 0.46* 0.32 Two-tailed test: * P<0.05; ** P<0.01. ==== Refs References 1 Zhang Y Proenca R Maffei M Barone M Leopold L Friedman JM Positional cloning of the mouse obese gene and its human homologue Nature 1994 372 425 431 7984236 2 Whitifield JF Leptin: brains and bones Exp Opin Invest Drugs 2001 10 1617 1622 3 Halaas JL Gajiwala KS Maffei M Cohen SL Chait BT Rabinowitz D Lallone RL Burley SK Friedman JM Weight reducing effects of the plasma protein encoded by the obese gene Science 1995 269 543 546 7624777 4 Caro JF Kolacznski JW Nyce MR Ohannesian JP Opentanova I Goldman WH Lynn RB Zhang PL Sinha MK Considine RV Decreased cerebrospinal-fluid/serum leptin ratio in obesity: a possible mechanism for leptin resistance Lancet 1996 348 159 161 8684156 5 Strobel A Issad T Camoin L Ozata M Strosberg AD A leptin missense mutation associated with hypogonadism and morbid obesity Nat Genet 1998 18 213 215 9500540 6 Farooqi IS Jebb SA Langmack G Lawrence E Cheetham CH Prentice AM Hughes IA Mc-Camish MA O’Rahilly S Effects of recombinant leptin therapy in a child with congenital leptin deficiency N Engl J Med 1999 341 879 884 10486419 7 Considine RV Sinha MK Heiman ML Kriaciunas A Stephens TW Nyce MR Ohannesian JP Marco CC McKee LJ Bauer TL Serum immunoreactive leptin concentrations in normal weight and obese humans N Engl J Med 1996 334 292 295 8532024 8 Ahrén B Plasma leptin and insulin in C57BI/6J mice on a high-fat diet: relation to subsequent changes in body weight Acta Physiol Scand 1999 165 233 240 10090336 9 Ghizzoni L Mastorakos G Interactions of leptin, GH, and cortisol in normal children Ann N Y Acad Sci 2003 977 56 63 10 Mantzoros CS Role of leptin in reproduction Ann NY Acad Sci 2000 900 174 183 10818404 11 Karlsson C Lindell K Svensson E Bergh C Lind P Billing H Carlsson LM Carlsson B Expression of functional leptin receptors in the human ovary J Clin Endoccrinol Metab 1997 82 4144 4148 12 Spicer L Francisco C The-adipose obese gene product-leptin: evidence of a direct inhibitory role in ovarian function Endocrinology 1997 138 3374 3379 9231790 13 Caprio M Fabbrini E Isidori AM Aversa A Fabbri A Leptin in reproduction Trends Endocrinol Metab 2001 12 65 72 11167124 14 Yu WH Klimura M Walczewska A Karanth S McCann SM Role of leptin in hypothalamicpituitary function Proc Natl Acad Sci USA 1997 94 1023 1028 9023376 15 Yu WH Walczewska A Karanth S McCann S Nitric oxide mediates leptin - 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