==== Front Invest Ophthalmol Vis SciInvest. Ophthalmol. Vis. SciiovsInvest Ophthalmol Vis SciIOVSInvestigative Ophthalmology & Visual Science0146-04041552-5783The Association for Research in Vision and Ophthalmology 10.1167/iovs.18-24558iovs-59-08-07IOVS-18-24558R1RetinaRhythmic Regulation of Photoreceptor and RPE Genes Important for Vision and Genetically Associated With Severe Retinal Diseases Daily Regulation of Visual GenesVancura Patrick 1Csicsely Erika 1Leiser Annalisa 1Iuvone P. Michael 2Spessert Rainer 1 1 Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany 2 Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia, United States Correspondence: Rainer Spessert, Department of Functional and Clinical Anatomy, University Medical Center of the Johannnes Gutenberg University Mainz, Saarstrasse 19-21, Mainz 55099, Germany; spessert@uni-mainz.de.8 2018 59 10 3789 3799 13 4 2018 11 6 2018 Copyright 2018 The Authors2018This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.Purpose The aim of the present study was to identify candidate genes for mediating daily adjustment of vision. Methods Genes important for vision and genetically associated with severe retinal diseases were tested for 24-hour rhythms in transcript levels in neuronal retina, microdissected photoreceptors, photoreceptor-related pinealocytes, and retinal pigment epithelium-choroid (RPE-choroid) complex by using quantitative PCR. Results Photoreceptors of wildtype mice display circadian clock-dependent regulation of visual arrestins (Arr1, Arr4) and the visual cycle gene Rdh12, whereas cells of the RPE-choroid exhibit light-dependent regulation of the visual cycle key genes Lrat, Rpe65, and Rdh5. Clock-driven rhythmicity of Arr1, Arr4, and Rdh12 was observed also in rat pinealocytes, to persist in a mouse model of diabetic retinopathy (db/db) and, in the case of Arr1, to be abolished in retinae of mice deficient for dopamine D4 receptors. Therefore, the expression rhythms appear to be evolutionary conserved, to be unaffected in diabetic retinopathy, and, for Arr1, to require dopamine signaling via dopamine D4 receptors. Conclusions The data of the present study suggest that daily adjustment of retinal function combines clock-dependent regulation of genes responsible for phototransduction termination (Arr1, Arr4) and detoxification (Rdh12) in photoreceptors with light-dependent regulation of genes responsible for retinoid recycling (Lrat, Rpe65, and Rdh5) in RPE. Furthermore, they indicate circadian and light-dependent regulation of genes genetically associated with severe retinal diseases. circadian regulationvisual cycleretinavisual arrestin ==== Body The mammalian retina has the ability to adjust its function to the marked daily changes in the environmental lighting conditions.1,2 This involves the adjustment of photoreception and visual processing3 that manifests in circadian changes in the retinal electrical responses to light, which can be measured using the ERG.4 Daily regulation of the retina might also be important to comply with daily changes in the occurrence of toxic light (elevated during the day) and oxidative stress (elevated at night). Accordingly, circadian output has been seen to affect photoreceptor viability and to promote ganglion cell survival during aging conditions.1 Daily adaptation of retina and photoreceptors is partly driven by the retina's own circadian clock system,5,6 where circadian clocks are localized in various types of retinal neurons, including photoreceptors.7–9 The circadian regulation of visual function involves the neurohormones melatonin acting on MT1 and MT2 receptors,10,11 and dopamine acting on D4 receptors12–15 as biochemical transducers of night and day, respectively. Vision in all vertebrates is initiated by the absorption of photons by the photoreceptive pigments rhodopsin (in rods) and opsin (in cones) located in the photoreceptor outer segments.16 In general, photons induce the conversion of the photopigment's covalently bound 11-cis retinal to all-trans-retinal, inducing a structural switch that activates the photopigment. The reactions responsible for inactivation of photoexcited photopigment include phosphorylation of the photopigment by rhodopsin kinase followed by the binding of arrestin1 (Arr1) in rods and probably arrestin4 (Arr4, also referred to as cone arrestin) in cones.17–21 The maintenance of vision requires the regeneration of 11-cis retinal.22,23 To meet this requirement, all-trans-retinal is reisomerized to 11-cis retinal, a process that is performed by a multistep enzyme pathway called the visual (retinoid) cycle. The first catalytic step of the visual cycle, namely the reduction of all-trans-retinal to all-trans-retinol takes place in photoreceptors and is performed by five isoforms of the enzyme retinoid dehydrogenase/reductase (RDH)24 that are Rdh8 (prRdh), Rdh11, Rdh13, Rdh14, and Dhrs3 (retSdr1). Detoxification of retinaldehydes that exceed the reductive capacity of the outer segment compartment of the visual cycle is conducted by another isoform of Rdh, Rdh12, and a retina-specific ABC transporter (Abca4).25,26 The remaining steps of the visual cycle are performed in cells of the adjacent retinal pigment epithelium (RPE) that are (1) the esterification of all trans-retinol to all trans-retinyl esters by Lrat, (2) the hydrolyzation and isomerization of all trans-retinyl esters to 11-cis retinol by Rpe65, and (3) the oxidation of 11-cis retinol to 11-cis retinal by the Rdh isoforms Rdh5, Rdh10, and Rdh11. Additional key players of the visual cycle are Rlbp1 (also referred to as Cralbp) and Rbp1 (also referred to as Crbp), which catalyze the transport of 11-cis retinal and retinol within the RPE compartment.26 Due to the compartmentalization of the visual cycle into different cell types, retinoid intermediates have to translocate across the interphotoreceptor matrix, a process mediated by the interphotoreceptor retinoid-binding protein (encoded by the gene Rbp3), which is synthesized and secreted by photoreceptors. Daily adjustment of vision involves 24-hour changes in the expression of Arr1 and Kcnv2, a channel essential for vision.27–30 The data included in the present study suggest that it also involves rhythmic regulation of cone Arr4 and key genes of the visual cycle in photoreceptors and RPE. Materials and Methods Animals Adult male and female mice (see below) and rats (Sprague Dawley) with intact photoreceptors not carrying rd mutations were used in this study. With the exception of the mouse model for diabetic retinopathy (C57BL/6Jb db/+, C57BL/6Jb db/db), the mice used were melatonin-proficient (C3H/f+/+, C3H/f+/+Drd4+/+ and C3H/f+/+Drd4−/−). Where indicated, mice deficient for dopamine D4 receptors (Drd4−/−) were used. Mice were genotyped by PCR analysis of genomic DNA. Diabetic (db/db) and nondiabetic (db/+) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA). They were checked for body-weight and blood glucose level by tail vein sampling using Accu-Check Aviva reagent strips (Roche Diagnostics, Mannheim, Germany) at the age of 10 weeks. Diabetic mice displayed enhanced values of blood glucose (397 ± 14 mg/dl) and bodyweight (46 ± 3 g) as compared with nondiabetic mice (blood glucose level, 138 ± 4 mg/dl; bodyweight, 25 ± 1 g). Animals were kept under light:dark (LD) 12:12 cycles for 3 weeks under standard laboratory conditions (illumination with 200 lux white light at cage level during the day and dim [<5 lux] red light during the night, 20°C ± 1°C, water and food ad libitum) and sacrificed at the age of 13 to 15 weeks at 3-hour intervals over a period of 24 hours by decapitation following anesthesia with carbon dioxide. In order to determine the putative clock-dependent regulation of genes, mice previously adapted to LD were housed in constant darkness (DD) for one cycle and sacrificed during the next cycle in DD. Animal experimentation was carried out in accordance with the National Institutes of Health Guide on the Care and Use of Laboratory Animals and the ARVO Statement for the Use of Animals in Ophthalmic Vision Research and approved by the Institutional Animal Care and Use Committees of Morehouse School of Medicine, Emory University, and the European Communities Council Directive (86/609/EEC). Retina and RPE-Choroid Sampling Eyes of mice were enucleated and, after removal of the optic nerve and connective tissue, were incised around the dentate border of the retina (ora serrata). After discarding the lens and vitreous, the neural retinas and adjacent RPE-choroid complexes were carefully separated and immediately frozen or, in the case of retinas, processed for laser microdissection and pressure catapulting (LMPC). The purity of the preparations obtained were verified by using specific gene markers of the RPE, namely Lrat and Rdh5, and the retina, namely Arr1 and Rdh12. In comparison to retina, enrichment of Lrat and Rdh5 was more than 150-fold in the RPE-choroid complex. In comparison to that of the RPE-choroid complex, enrichment of Arr1 and Rdh12 was more than 100-fold in retina. All dissections during the dark phase were done under dim red light. In order to prepare the neural retinas for LMPC, the HEPES-glutamic acid buffer-mediated organic solvent protection effect (HOPE) technique (DCS, Hamburg, Germany) was applied for fixation. In this procedure, fresh retinas were fixed in HOPE I (DCS) at 0°C to 4°C for 48 hours. Subsequently, dehydration of the retinas was performed with a mixture of HOPE II solution (DCS) and acetone for 2 hours at 0°C to 4°C, followed by dehydration in pure acetone at 0°C to 4°C (repeated twice). Tissues were then embedded with low-melting point paraffin (Tm, 52°C–54°C) and sectioned (10 μm) on membrane-mounted slides (DNase/RNase free PALM MembraneSlides; P.A.L.M. Microlaser Technologies GmbH, Bernried, Germany). Subsequently sections were deparaffinized with isopropanol (2 × 10 minutes each at 60°C), stained using cresyl violet (1% w/v cresyl violet acetate in 100% ethanol), briefly washed in 70% and 100% ethanol, and then air-dried. Isolation of Photoreceptor Cells To isolate photoreceptors (rod and cones) from the stained sections in a contact and contamination-free manner, LMPC was performed with a PALM MicroBeam system (Zeiss MicroImaging, Munich, Germany) running PALM RoboSoftware (P.A.L.M. Microlaser Technologies GmbH) as described previously.8 In brief, these cells were selected, cut and catapulted into the caps of 0.5-ml microfuge tubes with an adhesive filling (PALM AdhesiveCaps; P.A.L.M. Microlaser Technologies GmbH) by using a pulsed UV-A nitrogen laser under the 10× objective. To reach total average sample sizes of 4,000,000 μm2 per tube, smaller areas of the sections were pooled. The purity of the preparations obtained were verified by using specific gene markers of photoreceptors, namely neural retina leucine zipper (Nrl) as a marker for rods,31 as well as of inner retinal neurons, namely tyrosine hydroxylase (Th) as a marker for amacrine cells.32 In comparison with whole retina preparations, in photoreceptors collected by LMPC, the ratio of Nrl to Th was increased 84-fold. RNA Extraction, Reverse Transcription (RT), and Quantitative PCR (qPCR) Using the RNeasy Micro kit (Qiagen, Hilden, Germany), RNA was extracted from the tissue samples as described.33 The amount of extracted RNA was determined by measuring the optical density at 260 and 280 nm. Subsequently, single-stranded cDNA was synthesized by using the Verso cDNA Kit (Abgene, Hamburg, Germany), following the manufacturer's instructions. Briefly, 4 μl RNA solution was reverse transcribed using anchored oligo-dT primers in a final volume of 20 μl. Following dilution of the obtained cDNA sample in RNase-free water (1:4), qPCR, with aliquots of 5 μl being used, was performed. PCR amplification and quantification were carried out in duplicate using an i-Cycler (BioRad, Munich, Germany) according to the following protocol: denaturation for 30 seconds at 95°C, followed by 45 cycles of 5 seconds at 95°C and 30 seconds at 60°C. By using agarose gel electrophoresis, the generated amplicons for all genes under examination were shown to possess the predicted sizes (Table 1). The amount of mRNA in the samples was calculated from the measured threshold cycles (Ct) by using an internal standard curve with 10-fold serial dilutions (101–108 copies/μl). Expression levels of each transcript were normalized with respect to the amount of Gapdh mRNA and 18S rRNA present. Table 1 Primer Sequences Used for qPCR Statistical Analysis All data are expressed as the mean ± standard error of the mean (SEM) of four qPCR experiments from four independent tissue samples. Transcript levels were calculated relative to average expression of each dataset throughout 24 hours to plot temporal expression. Cosinor analysis was used to evaluate variations among the groups in the 24-hour profile and to fit sine-wave curves to the circadian data to mathematically estimate the time of peaking gene expression (acrophase) and to assess the amplitude.34,35 The model can be expressed according to the following equation: f(t) = A + B cos [2π (t + C)/T], with the f(t) indicating relative expression levels of target genes, t specifying the time of sampling (hours after light-on), A representing the mean value of the cosine curve (mesor; midline estimating statistic of rhythm), B indicating the amplitude of the curve (half of the sinusoid), and C indicating the acrophase (point of time, when the function f[t] is maximum). T gives the time of the period, which was fixed at 24 hours for this experimental setting. Significance of daily regulation was defined by showing a P < 0.05. Results Visual Arrestins and Rdh12 are Under Circadian Regulation in Neuronal Retina and Photoreceptors To investigate whether the neural retina (devoid of the RPE-choroid) and photoreceptors of mice display daily regulation of genes important for visual processing, 24-hour profiling of the mRNA levels of visual arrestins and enzymes of the photoreceptor compartment of the visual cycle was performed. Among the genes tested, Arr1, Arr4, and Rdh12 were seen to display significant daily rhythms in neural retina and photoreceptors of mouse (Fig. 1, blue and red lines; for statistical analysis, see Table 2). Peak expression occurred for Arr1 in retina at Zeitgeber time (ZT) 8.2 and in photoreceptors at ZT7.7, for Arr4 in retina at ZT12.5 and in photoreceptors at ZT11.4, and for Rdh12 in retina at ZT9.4 and in photoreceptors at ZT7.8. No daily periodicity was observed for the visual cycle genes Rdh8, Rdh11, Rdh13, Rdh14, Dhrs3, Abca4, and Rbp3. Figure 1 Daily rhythmicity of Arr1, Arr4, and Rdh12 in retina and photoreceptors in LD and under constant darkness. Transcript levels of the genes were monitored in comparison to the clock-controlled gene E4bp4 under LD 12:12 in mouse retina (blue lines) and in isolated mouse photoreceptors (red lines), and under constant darkness in mouse retina (black lines) by using qPCR. The mRNA levels are plotted as a function of ZT and circadian time (CT). The lines represent the periodic sinusoidal functions determined by cosinor analysis (solid line for P < 0.05 in cosinor analysis). Data represent a percentage of the average value of the transcript amount during the 24-hour period. Statistical analysis of transcriptional profiling is provided in Table 2. The value of ZT0 is plotted twice at both ZT0 and ZT24. The solid bars indicate the dark period. Each value represents mean ± SEM (n = 4; each n represents one animal [two retinas] for whole retina preparations and two animals [four retinas] for photoreceptor preparations). Table 2 Statistical Analysis of Transcriptional Profiling Illustrated in Figures 1 to 4 The daily rhythmicity of Arr1, Arr4, and Rdh12 may be driven by a true circadian clock or light/dark transitions. To test circadian regulation, 24-hour profiling of the genes was performed in mice kept for one cycle under constant darkness (Fig. 1, black lines; for statistical analysis, see Table 2). Consistent with the concept that daily rhythmicity of the genes is promoted by a true circadian clock, daily changes of the genes under investigation persisted in the absence of light/dark transitions. To test the validity of the experimental system used, the clock-controlled gene E4bp4 was recorded in the same transcriptomes as those used for analyzation of the other genes. Consistent with the validity of the results obtained, E4bp4 transcript amount was observed to be rhythmic in retina and photoreceptors, under LD 12:12 and DD (Fig. 1; for statistical analysis, see Table 2). Visual Arrestins and Rdh12 Are Also Rhythmic in Rat Pineal Gland Mammalian photoreceptors and pinealocytes phylogenetically and ontogenetically descend from a common ancestor cell type.36 To investigate whether rhythmicity of the genes is phylogenetically conserved, the 24-hour profiling of the genes was compared in retina and pinealocytes. Because the pineal gland is much larger in rat than in mice, rat tissue was used to obtain sufficient mRNA levels for this purpose. Arr1, Arr4, and Rdh12 were rhythmically expressed in rat pinealocytes with similar profiles as those observed in rat retina (Fig. 2, blue and red lines; for statistical analysis, see Table 2). Figure 2 Daily rhythmicity of Arr1, Arr4, and Rdh12 in rat pineal gland and rat retina. Transcript levels of the genes were monitored in comparison to the clock-controlled gene E4bp4 under LD 12:12 in pineal gland (blue lines) and retina (red lines) of rats by using qPCR. The mRNA levels are plotted as a function of ZT and the lines represent the periodic sinusoidal functions determined by cosinor analysis (solid line for P < 0.05 in cosinor analysis). Data represent a percentage of the average value of the transcript amount during the 24-hour period. Statistical analysis of transcriptional profiling is provided in Table 2. The value of ZT0 is plotted twice at both ZT0 and ZT24. The solid bars indicate the dark period. Each value represents mean ± SEM (n = 4; each n represents two retinas and a pineal gland of one animal). Consistent with the validity of the results obtained, the clock-controlled gene E4bp4 was observed to be rhythmic in the same transcriptomes as those used for profiling the other genes (Fig. 2, blue and red lines; for statistical analysis, see Table 2). Expression of Arr1 Is Arrhythmic in Dopamine D4 Receptor–Deficient Mice In order to evaluate the contribution of dopamine to circadian regulation, 24-hour profiling of the genes was performed in mice deficient for the D4 receptor (Fig. 3, blue versus red lines; for statistical analysis, see Table 2). Daily regulation of Arr1 was not observed in retina of Drd4-deficient mice, but Arr4 and Rdh12 were rhythmically expressed. This suggests that circadian regulation of Arr1, but not that of Arr4 and Rdh12, requires dopamine signaling via D4 receptors. Figure 3 Daily rhythmicity of Arr1, Arr4, and Rdh12 in retina deficient for dopamine D4 receptors. Transcript levels of the genes were recorded in wildtype mice (blue lines) versus mice deficient for dopamine D4 receptors (red lines) in preparations of the whole retina under LD 12:12 by using qPCR. The mRNA levels are plotted as a function of ZT. The lines represent the periodic sinusoidal functions determined by cosinor analysis (solid and broken line for P < 0.05 and P > 0.05). Data represent a percentage of the average value of the transcript amount during the 24-hour period. Statistical analysis of transcriptional profiling is provided in Table 2. Note that rhythmicity of Arr1 and E4bp4 is not observable in mice deficient for dopamine D4 receptors. The value of ZT0 is plotted twice at both ZT0 and ZT24. The solid bars indicate the dark period. Each value represents mean ± SEM (n = 4; each n represents one animal [two retinas]). As expected for a clock-controlled gene, E4bp4 rhythmicity was evident in the same transcriptomes as those used for analyzation of the other genes. Interestingly, E4bp4 rhythmicity mitigates in mice deficient for D4 receptors (Fig. 3, blue versus red lines; for statistical analysis, see Table 2). Because E4bp4 transcription is known to be directed by clock gene products,37 abolished E4bp4 rhythmicity may mirror an influence of dopamine signaling on the retinal clock function. Circadian Regulation of Visual Arrestins and Rdh12 Expression Persists in Diabetic Retinopathy In diabetic retinopathy, visual function is impaired. To investigate whether disturbed circadian control of Arr1, Arr4, and Rdh12 plays a role in this context, the db/db mice, a worldwide applied model of type II diabetes and diabetic retinopathy,38 was used. Irrespective of the melatonin deficiency of the db/db mouse (C57BL/6Jb background), the nondiabetic phenotype (db/+) was seen to display daily rhythms in Arr1, Arr4, and Rdh12 mRNA levels (Fig. 4, blue lines; for statistical analysis, see Table 2). This suggests that circadian regulation does not require melatonin signaling. Moreover, rhythmicity of the genes persisted in diabetic (db/db) mice (Fig. 4, blue versus red lines; for statistical analysis, see Table 2). Therefore, circadian regulation of Arr1, Arr4, and Rdh12 appears not to be affected in diabetic retinopathy. Figure 4 Daily rhythmicity of Arr1, Arr4, and Rdh12 in diabetic retina. Transcript levels of the genes were monitored in nondiabetic (db/+) mice (blue lines) versus diabetic (db/db) mice (red lines) in preparations of the whole retina under LD 12:12 by using qPCR. The mRNA levels are plotted as a function of ZT. The lines represent the periodic sinusoidal functions determined by cosinor analysis (solid and broken line for P < 0.05 and P > 0.05). Data represent a percentage of the average value of the transcript amount during the 24-hour period. Statistical analysis of transcriptional profiling is provided in Table 2. The value of ZT0 is plotted twice at both ZT0 and ZT24. The solid bars indicate the dark period. Each value represents mean ± SEM (n = 4; each n represents one animal [two retinas]). Consistent with the validity of the experimental system used, E4bp4 was observed to be rhythmic in the nondiabetic phenotype (db/+). The daily profile in the E4bp4 transcript was statistically arrhythmic in diabetic (db/db) mice, although it resembled that in nondiabetic (db/+) mice (Fig. 4, blue versus red lines; for statistical analysis, see Table 2). Visual Cycle Genes Are Under Daily Regulation in the RPE-Choroid Because the different steps of the visual retinoid cycle are diversified on photoreceptors and the adjacent RPE, and the visual cycle gene Rdh12 was seen to be rhythmic in mouse photoreceptors, possible daily fluctuations in the expression of genes of the RPE part of the visual cycle were investigated in preparations of the RPE-choroid obtained from mice. Among the genes tested, Lrat, Rpe65, and Rdh5 were seen to undergo daily rhythms with peaks at the early daytime (Lrat, ZT4.2; Rpe65, ZT1.3; Rdh5, ZT1.9) (Fig. 5, blue lines; for statistical analysis, see Table 3). No daily periodicity was evident for the visual cycle genes Rbp1, Rdh10, Rdh11, and Rlbp1. Figure 5 Daily rhythmicity of the visual cycle genes in RPE-choroid. Transcript levels of the visual cycle genes Lrat, Rpe65, and Rdh5 were monitored in comparison to the clock-controlled gene E4bp4 under LD 12:12 (blue lines) and constant darkness (black lines) in mouse RPE-choroid by using qPCR. The mRNA levels are plotted as a function of ZT and circadian time (CT). The lines represent the periodic sinusoidal functions determined by cosinor analysis (solid and broken line for P < 0.05 and P > 0.05). Data represent a percentage of the average value of the transcript amount during the 24-hour period. Statistical analysis of transcriptional profiling is provided in Table 3. The value of ZT0 is plotted twice at both ZT0 and ZT24. The solid bars indicate the dark period. Each value represents mean ± SEM (n = 4; each n represents one animal [two retinal pigment epithelia]). Table 3 Statistical Analysis of Transcriptional Profiling Illustrated in Figure 5 To check clock-dependent regulation of Lrat, Rpe65, and Rdh5, 24-hour profiling of the genes was performed in mice kept for one cycle under DD (Fig. 5, black lines; for statistical analysis, see Table 3). Consistent with the concept that daily rhythmicity of Lrat, Rpe65, and Rdh5 expression does not depend on a true circadian clock but requires LD transitions, daily periodicity of the transcripts vanished under DD. Consistent with the presence of a true circadian clock in RPE-choroid2,39 and the validity of the experimental system used, the clock-controlled gene E4bp4 was seen to display periodicity not only under LD 12:12 but also under DD (Fig. 5, blue versus black lines; for statistical analysis, see Table 3). Discussion In the present study, the genes Arr1, Arr4, Lrat, Rdh5, Rpe65, and Rdh12 were observed to display daily rhythms in either photoreceptors (Arr1, Arr4, and Rdh12) or RPE-choroid (Lrat, Rpe65, and Rdh5). Since they encode indispensable components of either the phototransduction pathway (Arr1, Arr4),16 or the visual retinoid cycle (Rdh12, Lrat, Rpe65, and Rdh5),22,23 two processes essential to vision, daily regulation appears to contribute to daily adjustment of vision to comply with 24-hour changes in lighting conditions. Remarkably, rhythmic regulation of the photoreceptor genes (Arr1, Arr4, and Rdh12) was seen to be driven by a circadian clock, whereas that of the RPE genes (Lrat, Rpe65, and Rdh5) was observed to depend on LD transitions. This suggests that daily adjustment of visual processing combines clock-driven gene regulation in photoreceptors with light-driven gene regulation in RPE. Circadian regulation of photoreceptor genes should derive from clocks located in photoreceptors, inner retinal neurons, and RPE-choroid39 but not from the master clock within the suprachiasmatic nucleus (SCN).2 Arr1 is abundant in rod and cone photoreceptors.18–21 Due to the limitation of the present study that photoreceptor transcript preparations derive from both rods and cones, Arr1 rhythms could reflect average rod and cone values and may not necessarily be valid for each type of photoreceptor. However, in the rod-dominant mouse retina, the observed 24-hour changes in Arr1 expression may mainly derive from rods. The phenotype of Arr1−/− mice suggests the necessity of this protein for phototransduction shutoff and light adaptation of rods.19,40 Therefore, circadian regulation of Arr1 may contribute to the reported daily changes in light adaptation.19 Different from Arr1, Arr4 expression is restricted to cone photoreceptors20,21 and, consequently, circadian regulation of Arr4 should occur in cones. Arr4−/− mice display visual defects, including decreased contrast sensitivity and visual acuity.20,21 This suggests that circadian regulation of the gene contributes to the observed 24-hour changes of these visual parameters.2,14,15 Cone Arr4 is evolutionarily conserved and its function is not redundant with that of Arr1.20,21 Accordingly, both types of visual arrestin appear to play complementary roles in the daily adjustment of retinal function. In rod and cone photoreceptors, protein formation occurs in the cell body and the inner segment. Therefore, increased transcription of the visual arrestins during the day may contribute to the accumulation of arrestin protein in these cell compartments at night. In response to light, both arrestins are translocated to the outer segment,41–45 where they influence phototransduction.46 Hence, circadian regulation of Arr1 and Arr4 may allow rods and cones to prepare an arrestin reservoir at night, ready for translocation to the outer segment in response to light. Lrat, Rpe65, and Rdh5 encode the key enzymes of the RPE compartment of the visual cycle.22 Therefore, concurrent upregulation of the genes during the daytime (this study) may result in a daytime peak in the capacity of the RPE to perform chromophore regeneration. This suggests that the 24-hour rhythms of Lrat, Rpe65, and Rdh5 complies with the requirement of the RPE to increase chromophore regeneration during light exposure/daytime. Rdh12 is localized to the inner segment of both rod and cone photoreceptors.47 It does not play a significant role in visual cycle function but is necessary to protect photoreceptors from toxic retinaldehydes that exceeds the reductive capacity of the outer segment compartment of the photoreceptor cells.48–50 Therefore, upregulation of the gene at the earlier time of day may fulfill the demand to protect the photoreceptors from increasing concentrations of retinaldehydes released during light exposure/daytime.51–53 Interestingly, the release of retinaldehydes during light exposure/daytime and thus the requirement for protection depends on Rpe65 activity.54,55 Therefore, upregulation of Rdh12 during the daytime may be necessary to compensate for an Rpe65-dependent increase in retinaldehyde release. The genes observed to be under daily regulation in the present study are important for maintaining vision and for protecting photoreceptors from cytotoxic byproducts of the visual pathways. Accordingly, mutations of these genes have been genetically linked to various forms of severe retinal diseases. Not only is Arr1 genetically associated to Oguchi disease56,57 and retinitis pigmentosa,58 but also are Lrat, Rpe65, and Rdh12 to Leber's congenital amaurosis.59 Mutations of Rdh5 are associated with fundus albipunctatus.60 Moreover, mice deficient for Arr1, Arr4, Rpe65, and Rdh12 suffer from dystrophy of rods (Arr1,61 Rpe65,62 and Rdh1252) and/or cones (Arr1,19 Arr4,20,21 Rpe65,63 and Rdh1252). These findings indicate that the abundance of each of the gene products is a prerequisite for retinal health. Thus, correct upregulation of the genes during early (Lrat, Rpe65, Rdh5, and Rdh12) or late (Arr1 and Arr4) daytime might be essential for retinal health. Rhythmicity of Arr1, Arr4, and Rdh12 persisted in the db/db mouse, a mouse model of diabetic retinopathy. Therefore, daily regulation of these genes might also be unaffected in diabetic retinopathy of humans, one of the most common causes of blindness in Europe and United States.64 Accordingly, the pathogenesis of diabetic retinopathy appears not to derive from disturbed circadian regulation of visual arrestins or Rdh12. Circadian regulation of Arr1 appears to be mediated by dopamine signaling via D4 receptors. This follows from the present observation that Arr1 periodicity is disrupted in retinas deficient of functional D4 receptors. D4 receptor-dependent control of photoreceptors12 appears to derive from the clock-driven release of dopamine from amacrine cells in the inner retina2 and/or from circadian expression of Drd4, the gene that encodes the dopamine D4 receptor.13 Therefore, circadian regulation of Arr1 in photoreceptors may be promoted by a molecular clock located within amacrine cells and photoreceptor cells. Daily regulation of Arr1, Arr4, and Rdh12 was seen in the present study to also occur in rat pineal gland, a neuroendocrine transducer of the circadian system.27,65 In mammalian pineal gland, rhythmicity of gene expression is driven by the master clock in the SCN.66 This suggests that regulation of the visual genes is circadian in retina and pineal gland but depends on different clocks, viz. the intraretinal clock system and the master clock in the SCN. Mammalian photoreceptors and pinealocytes phylogenetically and ontogenetically descend from a common ancestor cell type even if pinealocytes have lost direct photoreception and endogenous clock function during evolution.67 Therefore, the circadian regulation of the visual genes under investigation appears to be evolutionary conserved. In conclusion, the data of the present study suggest that genes important for phototransduction shutoff and retinoid renewal are not only important for maintaining vision, but also for mediating adjustment of vision to comply with 24-hour changes in lighting conditions. As a consequence, mutations of the respective genes might impair daily adjustment of the retina and this deficiency might contribute to the pathogenesis of the respective gene associated retinal disorders. Moreover, circadian regulation of Rdh12 may adjust the detoxification capacity of photoreceptors to changing amounts of cytotoxic byproducts of visual pathways. Therefore, Rdh12 is a candidate gene for mediating the positive influence of the retinal clock on photoreceptor survival.68 Acknowledgments The authors thank Ute Frederiksen and Kristina Schäfer for their excellent technical assistance, Susanne Rometsch and Bettina Wiechers-Schmied for secretarial help, and Russell G. Foster for providing us with C3H/f+/+ (rd++) mice. The data contained in this study are included in the theses of Patrick Vancura and Annalisa Leiser as a partial fulfillment of their doctoral degree and of Erika Csicsely as a partial fulfillment of her bachelor's degree at the Johannes Gutenberg University, Mainz, Germany. Supported in part by grants from the National Institutes of Health (R01EY004864, R01EY027711, and P30EY006360 to PMI) and by an unrestricted departmental grant from Research to Prevent Blindness to Emory Ophthalmology. Disclosure: P. Vancura, None; E. Csicsely, None; A. Leiser, None; P.M. Iuvone, None; R. Spessert, None ==== Refs References 1. Iuvone PM Tosini G. Pozdeyev N. Haque R. Klein DC Chaurasia SS. Circadian clocks, clock networks, arylalkylamine N-acetyltransferase, and melatonin in the retina Prog Retin Eye Res 2005 24 433 456 15845344 2. McMahon DG Iuvone PM Tosini G. Circadian organization of the mammalian retina: from gene regulation to physiology and diseases Prog Retin Eye Res 2014 39 58 76 24333669 3. Storch KF Paz C. Signorovitch J. Intrinsic circadian clock of the mammalian retina: importance for retinal processing of visual information Cell 2007 130 730 741 17719549 4. Cameron MA Barnard AR Hut RA Electroretinography of wild-type and Cry mutant mice reveals circadian tuning of photopic and mesopic retinal responses J Biol Rhythms 2008 23 489 501 19060258 5. Tosini G. Menaker M. The clock in the mouse retina: melatonin synthesis and photoreceptor degeneration Brain Res 1998 789 221 228 9573370 6. Kamphuis W. Cailotto C. Dijk F. Bergen A. Buijs RM. Circadian expression of clock genes and clock-controlled genes in the rat retina Biochem Biophys Res Commun 2005 330 18 26 15781226 7. Tosini G. Davidson AJ Fukuhara C. Kasamatsu M. Castanon-Cervantes O. Localization of a circadian clock in mammalian photoreceptors FASEB J 2007 21 3866 3871 17621597 8. Schneider K. Tippmann S. Spiwoks-Becker I. Unique clockwork in photoreceptor of rat J Neurochem 2010 115 585 594 20722965 9. Sandu C. Hicks D. Felder-Schmittbuhl MP. Rat photoreceptor circadian oscillator strongly relies on lighting conditions Eur J Neurosci 2011 34 507 516 21771113 10. Baba K. Pozdeyev N. Mazzoni F. Melatonin modulates visual function and cell viability in the mouse retina via the MT1 melatonin receptor Proc Natl Acad Sci U S A 2009 106 15043 15048 19706469 11. Sengupta A. Baba K. Mazzoni F. Localization of melatonin receptor 1 in mouse retina and its role in the circadian regulation of the electroretinogram and dopamine levels PLoS One 2011 6 e24483 21915336 12. Pozdeyev N. Tosini G. Li L. Dopamine modulates diurnal and circadian rhythms of protein phosphorylation in photoreceptor cells of mouse retina Eur J Neurosci 2008 27 2691 2700 18547251 13. Jackson CR Chaurasia SS Hwang CK Iuvone PM. Dopamine D(4) receptor activation controls circadian timing of the adenylyl cyclase 1/cyclic AMP signaling system in mouse retina Eur J Neurosci 2011 34 57 64 21676039 14. Jackson CR Ruan GX Aseem F. Retinal dopamine mediates multiple dimensions of light-adapted vision J Neurosci 2012 32 9359 9368 22764243 15. Hwang CK Chaurasia SS Jackson CR Chan GC Storm DR Iuvone PM. Circadian rhythm of contrast sensitivity is regulated by a dopamine-neuronal PAS-domain protein 2-adenylyl cyclase 1 signaling pathway in retinal ganglion cells J Neurosci 2013 33 14989 14997 24048828 16. Ingram NT Sampath AP Fain GL. Why are rods more sensitive than cones? J Physiol 2016 594 5415 5426 27218707 17. Chan S. Rubin WW Mendez A. Functional comparisons of visual arrestins in rod photoreceptors of transgenic mice Invest Ophthalmol Vis Sci 2007 48 1968 1975 17460248 18. Nikonov SS Brown BM Davis JA Mouse cones require an arrestin for normal inactivation of phototransduction Neuron 2008 59 462 474 18701071 19. Brown BM Ramirez T. Rife L. Craft CM. Visual arrestin 1 contributes to cone photoreceptor survival and light adaptation Invest Ophthalmol Vis Sci 2010 51 2372 2380 20019357 20. Deming JD Pak JS Brown BM Visual cone arrestin 4 contributes to visual function and cone health Invest Ophthalmol Vis Sci 2015 56 5407 5416 26284544 21. Deming JD Pak JS Shin JA Arrestin 1 and cone arrestin 4 have unique roles in visual function in an all-cone mouse retina Invest Ophthalmol Vis Sci 2015 56 7618 7628 26624493 22. Kiser PD Golczak M. Maeda A. Palczewski K. Key enzymes of the retinoid (visual) cycle in vertebrate retina Biochim Biophys Acta 2012 1821 137 151 21447403 23. Kiser PD Palczewski K. Retinoids and retinal diseases Annu Rev Vis Sci 2016 2 197 234 27917399 24. Parker RO Crouch RK. The interphotoreceptor retinoid binding (IRBP) is essential for normal retinoid processing in cone photoreceptors Adv Exp Med Biol 2010 664 141 149 20238012 25. Moradi P. Mackay D. Hunt DM Moore AT. Focus on molecules: retinol dehydrogenase 12 (RDH12) Exp Eye Res 2008 87 160 161 18599039 26. Liu X. Chen J. Liu Z. Li J. Yao K. Wu Y. Potential therapeutic agents against retinal diseases caused by aberrant metabolism of retinoids Invest Ophthalmol Vis Sci 2016 57 1017 1030 26962698 27. Craft CM Whitmore DH Donoso LA. Differential expression of mRNA and protein encoding retinal and pineal S-antigen during the light/dark cycle J Neurochem 1990 55 1461 1473 2213004 28. Agarwal N. Nir I. Papermaster DS. Loss of diurnal arrestin gene expression in rds mutant mouse retinas Exp Eye Res 1994 58 1 8 8157095 29. McGinnis JF Austin BJ Stepanik PL Lerious V. Light-dependent regulation of the transcriptional activity of the mammalian gene for arrestin J Neurosci Res 1994 38 479 482 7932878 30. Holter P. Kunst S. Wolloscheck T. The retinal clock drives the expression of Kcnv2, a channel essential for visual function and cone survival Invest Ophthalmol Vis Sci 2012 53 6947 6954 22969075 31. Mears AJ Kondo M. Swain PK Nrl is required for rod photoreceptor development Nat Genet 2001 29 447 452 11694879 32. Osborne NN Beaton DW Vigny A. Neuhoff V. Localization of tyrosine-hydroxylase immunoreactive cells in rabbit retinal cultures Neurosci Lett 1984 50 117 120 6149500 33. Kunst S. Wolloscheck T. Holter P. Transcriptional analysis of rat photoreceptor cells reveals daily regulation of genes important for visual signaling and light damage susceptibility J Neurochem 2013 124 757 769 23145934 34. Refinetti R. Lissen GC Halberg F. Procedures for numerical analysis of circadian rhythms Biol Rhythm Res 2007 38 275 325 23710111 35. Cornelissen G. Cosinor-based rhythmometry Theor Biol Med Model 2014 11 16 24725531 36. Rath MF Rohde K. Klein DC Moller M. Homeobox genes in the rodent pineal gland: roles in development and phenotype maintenance Neurochem Res 2013 38 1100 1112 23076630 37. Mitsui S. Yamaguchi S. Matsuo T. Ishida Y. Okamura H. Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism Genes Dev 2001 15 995 1006 11316793 38. Yang Q. Xu Y. Xie P. Retinal neurodegeneration in db/db mice at the early period of diabetes J Ophthalmol 2015 2015 757412 25821591 39. Baba K. Sengupta A. Tosini M. Contreras-Alcantara S. Tosini G. Circadian regulation of the PERIOD 2::LUCIFERASE bioluminescence rhythm in the mouse retinal pigment epithelium-choroid Mol Vis 2010 16 2605 2611 21151601 40. Xu J. Dodd RL Makino CL Simon MI Baylor DA Chen J. Prolonged photoresponses in transgenic mouse rods lacking arrestin Nature 1997 389 505 509 9333241 41. Broekhuyse RM Tolhuizen EF Janssen AP Winkens HJ. Light induced shift and binding of S-antigen in retinal rods Curr Eye Res 1985 4 613 618 2410196 42. Mirshahi M. Thillaye B. Tarraf M. de Kozak Y. Faure JP. Light-induced changes in S-antigen (arrestin) localization in retinal photoreceptors: differences between rods and cones and defective process in RCS rat retinal dystrophy Eur J Cell Biol 1994 63 61 67 8005106 43. Zhu X. Li A. Brown B. Weiss ER Osawa S. Craft CM. Mouse cone arrestin expression pattern: light induced translocation in cone photoreceptors Mol Vis 2002 8 462 471 12486395 44. Zhang H. Cuenca N. Ivanova T. Identification and light-dependent translocation of a cone-specific antigen, cone arrestin, recognized by monoclonal antibody 7G6 Invest Ophthalmol Vis Sci 2003 44 2858 2867 12824223 45. Haire SE Pang J. Boye SL Light-driven cone arrestin translocation in cones of postnatal guanylate cyclase-1 knockout mouse retina treated with AAV-GC1 Invest Ophthalmol Vis Sci 2006 47 3745 3753 16936082 46. Calvert PD Strissel KJ Schiesser WE Pugh EN Jr, Arshavsky VY. Light-driven translocation of signaling proteins in vertebrate photoreceptors Trends Cell Biol 2006 16 560 568 16996267 47. Kurth I. Thompson DA Ruther K. Targeted disruption of the murine retinal dehydrogenase gene Rdh12 does not limit visual cycle function Mol Cell Biol 2007 27 1370 1379 17130236 48. Chrispell JD Feathers KL Kane MA Rdh12 activity and effects on retinoid processing in the murine retina J Biol Chem 2009 284 21468 21477 19506076 49. Marchette LD Thompson DA Kravtsova M. Ngansop TN Mandal MN Kasus-Jacobi A. Retinol dehydrogenase 12 detoxifies 4-hydroxynonenal in photoreceptor cells Free Radic Biol Med 2010 48 16 25 19686838 50. Chen C. Thompson DA Koutalos Y. Reduction of all-trans-retinal in vertebrate rod photoreceptors requires the combined action of RDH8 and RDH12 J Biol Chem 2012 287 24662 24670 22621924 51. Maeda A. Golczak M. Maeda T. Palczewski K. Limited roles of Rdh8, Rdh12, and Abca4 in all-trans-retinal clearance in mouse retina Invest Ophthalmol Vis Sci 2009 50 5435 5443 19553623 52. Maeda A. Maeda T. Imanishi Y. Retinol dehydrogenase (RDH12) protects photoreceptors from light-induced degeneration in mice J Biol Chem 2006 281 37697 37704 17032653 53. Mackay DS Dev Borman A. Moradi P. RDH12 retinopathy: novel mutations and phenotypic description Mol Vis 2011 17 2706 2716 22065924 54. Maiti P. Kong J. Kim SR Sparrow JR Allikmets R. Rando RR. Small molecule RPE65 antagonists limit the visual cycle and prevent lipofuscin formation Biochemistry 2006 45 852 860 16411761 55. Kiser PD Zhang J. Badiee M. Rational tuning of visual cycle modulator pharmacodynamics J Pharmacol Exp Ther 2017 362 131 145 28476927 56. Nakamachi Y. Nakamura M. Fujii S. Yamamoto M. Okubo K. Oguchi disease with sectoral retinitis pigmentosa harboring adenine deletion at position 1147 in the arrestin gene Am J Ophthalmol 1998 125 249 251 9467455 57. Hayashi T. Tsuzuranuki S. Kozaki K. Urashima M. Tsuneoka H. Macular dysfunction in Oguchi disease with the frequent mutation 1147delA in the SAG gene Ophthalmic Res 2011 46 175 180 21447990 58. Nakazawa M. Wada Y. Tamai M. Arrestin gene mutations in autosomal recessive retinitis pigmentosa Arch Ophthalmol 1998 116 498 501 9565049 59. Chacon-Camacho OF Zenteno JC. Review and update on the molecular basis of Leber congenital amaurosis World J Clin Cases 2015 3 112 124 25685757 60. Skorczyk-Werner A. Pawlowski P. Michalczuk M. Fundus albipunctatus: review of the literature and report of a novel RDH5 gene mutation affecting the invariant tyrosine (p.Tyr175Phe) J Appl Genet 2015 56 317 327 25820994 61. Song X. Seo J. Baameur F. Rapid degeneration of rod photoreceptors expressing self-association-deficient arrestin-1 mutant Cell Signal 2013 25 2613 2624 24012956 62. Metrailler S. Schorderet DF Cottet S. Early apoptosis of rod photoreceptors in Rpe65(-/-) mice is associated with the upregulated expression of lysosomal-mediated autophagic genes Exp Eye Res 2012 96 70 81 22227450 63. Rohrer B. Lohr HR Humphries P. Redmond TM Seeliger MW Crouch RK. Cone opsin mislocalization in Rpe65-/- mice: a defect that can be corrected by 11-cis retinal Invest Ophthalmol Vis Sci 2005 46 3876 3882 16186377 64. Stitt AW Lois N. Medina RJ Adamson P. Curtis TM. Advances in our understanding of diabetic retinopathy Clin Sci (Lond ) 2013 125 1 17 23485060 65. Hastings MH Maywood ES Reddy AB. Two decades of circadian time J Neuroendocrinol 2008 20 812 819 18601704 66. Bailey MJ Coon SL Carter DA Night/day changes in pineal expression of >600 genes: central role of adrenergic/cAMP signaling J Biol Chem 2009 284 7606 7622 19103603 67. Maronde E. Stehle JH. The mammalian pineal gland: known facts, unknown facets Trends Endocrinol Metab 2007 18 142 149 17374488 68. Ait-Hmyed O. Felder-Schmittbuhl MP Garcia-Garrido M. Mice lacking Period 1 and Period 2 circadian clock genes exhibit blue cone photoreceptor defects Eur J Neurosci 2013 37 1048 1060 23351077