==== Front BMJ OpenBMJ OpenbmjopenbmjopenBMJ Open2044-6055BMJ Publishing Group BMA House, Tavistock Square, London, WC1H 9JR bmjopen-2017-02060910.1136/bmjopen-2017-020609PaediatricsResearch150617191334Establishment of blood pressure nomograms representative for Egyptian children and adolescents: a cross-sectional study El-shafie Ali M 1El-Gendy Fady M 1Allhony Dalia M 1Abo El Fotoh Wafaa Moustafa M 1Omar Zein A 1Samir Mohamed A 1Bahbah Wael A 1Abd el naby Sameh Abdallah 1El Zayat Rania S 1Abd El Hady Nahla M Said 1El Gazar Basim A 1Zannoun Mohamed A 2Kasemy Zeinab A 3El-Bazzar Ahmed N 4Abd El-Fattah Mohamed Abd El-nour 4Abd El-monsef Amir A 4Kairallah Amir M 4Raafet Hythem M 4Baz Ghada M 4Salah Amany Gaber 4Galab Walaa S 4 1 Department of Pediatrics, Faculty of Medicine, Menoufia University, Shebin El-Kom, Egypt 2 Department of Paediatrics, Faculty of Medicine, Al-Azhar University, Cairo, Egypt 3 Department of Public Health and Community Epidemiology and Biostatistics Division, Faculty of Medicine, Menoufia University, Shebin El-Kom, Egypt 4 Ministry of Health Hospitals, Cairo, Egypt Correspondence to Professor Wafaa Moustafa M Abo El Fotoh; wafaamoustafa60@yahoo.com2018 25 7 2018 8 7 e02060915 11 2017 06 6 2018 12 6 2018 © Author(s) (or their employer(s)) 2018. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.2018This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.Objective To define nomograms for blood pressure in Egyptian children and adolescents. Methods and study design A total of 60 025 Egyptian children from birth to 19 years were enrolled in this cross-sectional randomised study from December 2015 to March 2017. They were selected from diverse geographical districts in Egypt. Healthy children who fulfilled the inclusion criteria, which included good nutritional history, absence of fever or documented underlying disease at the time of examination, no evidence of haemodynamically significant illness, and no antihypertensive drugs or other chronic drug administration, were included in the study. Body weight, recumbent length (for less than 24 months) and height (from 2 years to 19 years), and blood pressure were measured using standard mercury sphygmomanometers. Results Blood pressure increases with age in both boys and girls. The 90th percentile of systolic and diastolic blood pressure among Egyptian children was different from other ethnic populations (American and Turkish children) in both sexes. Systolic and diastolic blood pressure showed a positive correlation with weight and height in both sexes (p<0.001). Conclusion We assumed that normal blood pressure curves should be used cautiously during childhood, and it is recommended that every population have its own normal standard curve to define measured blood pressure levels in children. These centiles increased our knowledge and awareness of normal blood pressure among Egyptian children and adolescents. The percentiles will distinguish children and young adolescents with increased blood pressure and will be of value to both medical practice and scientific research. EgyptianBlood pressureNomogramsChildrenAdolescencespecial-featureunlocked ==== Body Strengths and limitations of this study The study has appropriate sample size estimation. Another strength of the study is its multicentre representation of diverse geographical districts in Egypt. The study used standard accurate mercury sphygmomanometers to record blood pressure (BP). BP measurements were obtained in usual settings for children such as school and home, overcoming the phenomenon of white coat hypertension. This is the first study to specify BP values and curves in Egyptian boys and girls. Introduction Blood pressure (BP) is regulated by an array of physiological mechanisms, including neuronal and hormonal impulses of the heart, blood vessels, brain, renal system and digestive tissues.1 Age is considered one of the main determinants of BP values. BP increases more and more with age and more precipitously during puberty.2 The additional contributing factor is sex, as the occurrence of hypertension is higher among boys than girls. BP values, particularly beyond 12–14 years, are greater in boys than girls, and this is similar to the findings in adults.3 As with adults, BP in children is determined by both height and weight; however, in the paediatric setting, the fraction attributable to height may be physiological, and may not be influenced by excess weight.4 There is considerable proof that BP measured in childhood predicts future BP. Those with BP levels in the higher sections of the distribution curve have a tendency to sustain this level over time, which is uncovering of BP tracking.5 Hence, BP estimation has to be included in regular physical assessments during childhood and elucidated according to the standards of the normal childhood BP distribution curve.6 The distribution of BP levels and hypertension differs in various racial and ethnic clusters. This difference is determined by multiple factors, both genetic and environmental determinants. Grounded on these disparities, reference standards established for one population may not be appropriate in others.2 7–12 Regional native reference norms are needed to evaluate estimated BP values. Thus, this work aimed to establish representative BP percentile charts for Egyptian children and adolescents for the early detection and accurate recognition of BP fluctuations and/or abnormalities. Methods and design Participants Egypt is a great country consists of 27 governorates. The multistage random sampling technique was used to conduct the study. Out of the 27 governorates, 2 big governorates from Upper Egypt and 6 big governorates from Lower Egypt were chosen. Then 20 districts and cities were randomly chosen out of the previously chosen governorates. Within the districts and cities, facilities including a nursery, Primary care units (PCU), vaccination centres, basic education schools and secondary training schools were counted, and 160 facilities in the selected 8 governorates were randomly chosen, as demonstrated in the sampling flow chart (online supplementary file). The study was conducted 3 days a week. The crowded days were taken in order to obtain and gather more information. In the nursery, primary health care (PHC) units and vaccination centres, the study was conducted in the morning in private rooms immediately after receiving medical advice or vaccination. In schools, the measurements were taken between the educational courses, during breaktime. The total number of children who were eligible for the study was 64 500. After applying the inclusion and exclusion criteria, 4475 children were excluded, giving a final total sample of 60 025. The children who fulfilled the criteria of the study from birth to 19 years were examined from December 2015 to March 2017, and are representative of the children in Egypt. Verbal consents were obtained from parents or guardians, and they were informed about the objectives of the study, its benefits and the absence of any risk associated with the participation of their children. 10.1136/bmjopen-2017-020609.supp1Supplementary file 1 Inclusion and exclusion criteria Healthy children who fulfilled the inclusion criteria, which included good nutritional history, absence of fever or documented underlying disease at the time of examination, no evidence of haemodynamically significant illness such as congenital heart disease, or thoracic surgery, and no antihypertensive drugs or other chronic drug administration, were included. Children and adolescents with obesity were excluded. There were 807 obese children: 338 boys (9 from birth to 1 year and 329 from 1 year to 19 years) and 469 girls (12 from birth to 1 year and 457 from 1 year to 19 years). Measurements and data collection Records were assembled by qualified medical personnel. Weight, recumbent length (for less than 24 months) and height (from 2 years to 19 years) were measured, as well as BP. All BP measuring instruments were calibrated on a daily basis. Standard mercury sphygmomanometers (Model 1002/Presameter, Riester, Germany) with different cuff sizes were used for all measurements.13 In the present study, 60 025 children were examined in 160 facilities in 8 governorates using the standard mercury sphygmomanometer. Training was conducted with the working team for 3 days, along with invited children of different age and gender. This training was followed by testing to assess the degree of response to training and the quality of the measurement. The mean differences among the working observer team on 100 children of different age and sex as a pilot study were small (±0–3 mm Hg). The evaluation of the effectiveness of site visits was concluded, and the use of an electronic device for internal standardisation was described. In all facilities, reliability was high and comparable with the observers at the coordinating centre. All the participants were in a comfortable sitting position (infants in supine), in a wakeful state with their right arm fully exposed and resting on a supportive surface at the heart level. A cuff bladder was selected with a width that covered at least two-thirds of the upper arm (the distance between the olecranon and acromion) and a length beyond at best 80% of the biceps’ circumference. The cuff was inflated to a level that occluded the pulse at the wrist, the stethoscope was placed over the antecubital fossa, and then the cuff was deflated. The onset of the first Korotkoff (K1) sound was used as a measure for systolic blood pressure (SBP); K4 diastolic blood pressure (DBP) was used as the standard for infants and children 3–12 years of age; and K5 DBP was used as the standard for adolescents 13–19 years of age. Two readings were recorded with an interval of 5–10 min in-between and the mean was calculated for the final analysis. Patient and public involvement To improve the relevance of research, research orientation that includes patients and the public is vital. The authors aimed to identify the most important research priorities of patients, caregivers and healthcare providers (paediatricians, nurses and dietitians) for BP measurement. A paper-based survey asked patients, caregivers and care providers to submit their unanswered questions on BP measurement. A priority-setting process then ranked the final top 4 research priorities during an inperson meeting. There were 200 respondents who submitted 360 questions after exclusions. Of the respondents, 50% were patients or caregivers, 60% lived in urban areas, the caregivers were aged 23–45 years, and 85% were women. The 360 questions were reduced to 20 unique questions, and from this list the top 4 research questions that were prioritised included charts that can easily determine normal and abnormal levels of BP, education tools and technologies to improve patient motivation and health behaviours, evaluation of the optimal role of different healthcare providers and caregivers in supporting patients with abnormal change in BP, and development of healthy lifestyle modifications to reduce changes in BP. In areas that are of high priority for BP measurement, these priorities can be used to guide clinicians, researchers and funding bodies for patients, caregivers and healthcare providers. This also highlights priority areas for improved communication, knowledge transfer and delivery of patient-centred care. The children (n=100) of different age and sex who were subjected to BP measurement by observers to assess the degree of response to training and quality of measurement participated in setting the priorities of the research either on their own or by their caregiver. Some of these children, as well as caregivers, in addition to a large number of children from different sites in different governorates, helped us distribute the brochures that encourage BP measurement and invite their peers, for example, from schools, clubs and others, to come to the facilities where BP measurements were conducted. Also, we responded to some of the opinions of the caregivers, especially in addressing concerns with the great governorates and how to make communication with every single governorate, especially that for some of us it was the first time to visit some of the involved governorates. Their help will never be forgotten, so besides our role in the dissemination of the results through telephone calls to inform them about their BP measurements, we will also organise an event or a special day in every governorate to thank them and to address the main health problems they have. Statistical analysis With a view to accomplishing the reference standard curves (smoothed percentiles), logarithmic and semilogarithmic adjustments of SBP and DBP were statistically valued by regression analysis for different percentiles (50th, 75th, 90th and 95th), and the correlation coefficients of SBP and DBP with weight and height were processed. The statistical analysis was accomplished using IBM SPSS Statistics V.20 and ages were verified in complete years. The benchmarks used to certify the normal (50th–90th percentile), high-normal (90th–95th percentile) and high BP (>95th percentile) were comparable with the Report of the Second Task Force on Blood Pressure Control in Children in 1987.13 Results A total of 60 025 Egyptian children, 31 603 boys (52.6%) and 28 422 girls (47.4%), from birth to 19 years of age were examined in this study on SBP and DBP (table 1). Various smoothed percentile charts of SBP and DBP for both sexes according to age are shown in figures 1–8. In the present study, SBP and DBP rose steadily with age in both boys and girls. The mean annual increase in SBP for boys was 1.68 mm Hg and 1.53 mm Hg for girls (tables 2–5). The SBP increment was 2 mm Hg up to the age of 11 years in girls, and then decreased to 1 mm Hg from 12 to 19 years as the girls showed a sudden increase in height at puberty (table 5). Table 1 Age groups with the mean weight and height for both boys and girls Age (years) Boys Girls n Mean weight (kg) Deviations Mean height (cm) Deviations n Mean weight (kg) Deviations Mean height (cm) Deviations 1 1344 10 0.10991 75 2.43733 1348 9 0.12203 73 2.78564 2 5716 13 0.11425 87 3.29913 5685 12 0.12523 85 3.57202 3 2406 15 0.12023 95 3.75950 2333 15 0.12899 94 3.77889 4 2223 17 0.12603 103 4.13071 2148 17 0.13145 102 4.28939 5 2199 19 0.13555 109 4.77980 2135 18 0.14005 109 4.65815 6 1259 21 0.14399 115 4.96748 1096 21 0.15255 114 5.31711 7 1288 23 0.15487 121 5.34940 1082 23 0.16145 120 5.39606 8 1290 25 0.16588 127 5.86112 1050 25 0.17122 126 5.76132 9 1244 28 0.17211 132 5.94979 1026 29 0.17455 132 6.03576 10 1283 31 0.17781 137 6.28514 1088 32 0.17933 138 6.26325 11 1305 35 0.18022 142 6.93480 1030 35 0.18015 144 7.22371 12 1254 39 0.18253 148 7.11191 1047 40 0.18244 151 6.91346 13 1284 44 0.18972 155 7.43915 1063 44 0.18475 156 7.02548 14 1247 48 0.18881 162 7.69325 1053 47 0.18952 159 6.93250 15 1299 50 0.19035 168 7.64829 1036 49 0.19134 161 6.82588 16 1261 52 0.19683 172 7.88363 1049 52 0.19735 162 6.52740 17 1248 53 0.19874 175 7.51475 1.073 52 0.20175 162 6.39211 18 1210 53 0.20051 175 7.31851 1034 54 0.24520 162 6.15977 19 1243 54 0.20472 176 7.25872 1046 54 0.25780 162 6.47949 Total 31 603 – – 28 422 – – Figure 1 Smoothed percentile of systolic blood pressure (BP) measurements in Egyptian boys from birth to 24 months of age. Figure 2 Smoothed percentile of systolic blood pressure (BP) measurements in Egyptian boys from 1 to 19 years of age. Figure 3 Smoothed percentile of systolic blood pressure (BP) measurements in Egyptian girls from birth to 24 months of age. Figure 4 Smoothed percentiles of systolic blood pressure (BP) measurements in Egyptian girls from 1 to 19 years of age. Figure 5 Smoothed percentiles of diastolic blood pressure (BP) measurements in Egyptian boys from birth to 24 months of age. Figure 6 Smoothed percentiles of diastolic blood pressure (BP) measurements in Egyptian boys from 1 to 19 years of age. Figure 7 Smoothed percentiles of diastolic blood pressure (BP) measurements in Egyptian girls from birth to 24 months of age. Figure 8 Smoothed percentiles of diastolic blood pressure (BP) measurements in Egyptian girls from 1 to 19 years of age. Table 2 Exact percentile values for systolic blood pressure (SBP) measurements in Egyptian boys from birth to 24 months of age Age (month) Percentile values for SBP (mm Hg) 50% 75% 90% 95% 0 88 94 101 104 1 88 94 101 104 2 88 94 101 104 3 88 94 101 104 4 89 95 102 105 5 89 95 102 105 6 89 95 102 105 7 89 95 102 105 8 90 96 103 106 9 90 96 103 106 10 90 96 103 106 11 90 96 103 106 12 91 97 104 107 13 91 97 104 107 14 91 97 104 107 15 91 97 104 108 16 92 98 105 108 17 92 98 105 108 18 92 98 105 108 19 92 98 105 109 20 93 98 106 109 21 93 99 106 109 22 93 99 106 110 23 93 99 106 110 24 93 99 106 110 Table 3 Exact percentile values for systolic blood pressure (SBP) measurements in Egyptian boys from 1 to 19 years of age Age (year) Percentile values for SBP (mm Hg) 50% 75% 90% 95% 1 91 97 104 107 2 93 99 106 110 3 95 102 108 112 4 97 103 110 114 5 99 105 112 115 6 101 107 113 117 7 102 108 115 119 8 103 110 116 120 9 105 111 118 121 10 107 113 120 123 11 109 115 122 125 12 110 116 123 126 12 111 117 124 127 14 112 118 125 129 15 114 120 127 130 16 116 122 128 132 17 118 124 131 135 18 121 127 133 137 19 123 129 135 139 Table 4 Exact percentile values for systolic blood pressure (SBP) measurements in Egyptian girls from birth to 24 months of age Age (month) Percentile values for SBP (mm Hg) 50% 75% 90% 95% 0 88 95 100 104 1 88 95 100 104 2 88 95 101 105 3 88 95 101 105 4 89 96 101 105 5 89 96 102 106 6 89 96 102 106 7 89 96 102 106 8 90 97 102 107 9 90 97 103 107 10 90 97 103 107 11 90 97 103 107 12 91 98 104 108 13 91 98 104 108 14 91 98 104 108 15 91 98 104 108 16 92 99 105 109 17 92 99 105 109 18 92 99 105 110 19 92 99 106 110 20 93 100 106 110 21 93 100 106 110 22 93 100 107 111 23 93 100 107 111 24 93 100 107 111 Table 5 Exact percentile values for systolic blood pressure (SBP) measurements in Egyptian girls from 1 to 19 years of age Age (year) Percentile values for SBP (mm Hg) 50% 75% 90% 95% 1 91 98 104 108 2 93 100 107 111 3 96 103 109 115 4 98 105 112 117 5 100 107 114 119 6 102 109 115 121 7 103 111 117 123 8 105 112 119 124 9 107 114 120 126 10 109 116 122 128 11 111 118 124 130 12 112 119 125 131 12 113 120 126 132 14 114 121 127 133 15 115 122 128 134 16 116 123 129 135 17 117 124 130 136 18 118 125 131 137 19 119 125 132 137 The average annual increases in DBP for boys and girls were 0.95 mm Hg and 0.74 mm Hg, respectively. There was a sharp increase in DBP from 17 to 19 years of age (tables 6–9). Table 6 Exact percentile values for diastolic blood pressure (DBP) measurements in Egyptian boys from birth to 24 months of age Age (month) Percentile values for DBP (mm Hg) 50% 75% 90% 95% 0 52 58 63 67 1 52 58 63 67 2 52 58 63 67 3 52 58 63 67 4 53 59 64 68 5 53 59 64 68 6 53 59 64 68 7 53 59 64 68 8 54 60 65 69 9 54 60 65 69 10 54 60 65 69 11 54 60 65 69 12 55 61 66 70 13 55 61 66 70 14 55 61 66 70 15 55 61 66 70 16 56 62 67 71 17 56 62 67 71 18 56 62 67 71 19 56 62 67 71 20 57 63 68 71 21 57 63 68 72 22 57 63 68 72 23 57 63 68 72 24 57 63 68 72 Table 7 Exact percentile values for diastolic blood pressure (DBP) measurements in Egyptian boys from 1 to 19 years of age Age (year) Percentile values for DBP (mm Hg) 50% 75% 90% 95% 1 57 63 68 72 2 55 61 66 70 3 58 64 70 73 4 60 66 71 75 5 61 67 72 76 6 62 68 73 77 7 63 69 74 78 8 64 70 75 79 9 65 71 76 79 10 65 71 77 80 11 66 72 77 80 12 66 72 78 81 13 67 73 78 81 14 67 73 79 82 15 68 74 79 82 16 68 74 80 83 17 69 75 80 84 18 70 76 81 86 19 71 77 82 88 Table 8 Exact percentile values for diastolic blood pressure (DBP) measurements in Egyptian girls from birth to 24 months of age Age (month) Percentile values for DBP (mm Hg) 50% 75% 90% 95% 0 52 58 63 67 1 52 58 64 67 2 52 58 64 67 3 52 59 64 67 4 53 59 65 68 5 53 59 65 68 6 53 59 65 68 7 53 59 66 68 8 54 60 66 69 9 54 60 66 69 10 54 60 66 69 11 54 60 66 69 12 55 61 67 70 13 55 61 67 70 14 55 61 67 70 15 55 61 67 70 16 56 61 68 70 17 56 62 68 71 18 56 62 68 71 19 56 62 68 71 20 57 62 69 71 21 57 63 69 72 22 57 63 69 72 23 57 63 69 72 24 57 63 69 72 Table 9 Exact percentile values for diastolic blood pressure (DBP) measurements in Egyptian girls from 1 to 19 years of age Age (year) Percentile values for DBP (mm Hg) 50% 75% 90% 95% 1 55 61 67 70 2 57 63 69 72 3 59 65 71 74 4 61 67 73 76 5 62 68 74 77 6 63 69 75 78 7 64 70 76 79 8 65 70 77 80 9 65 71 77 80 10 66 71 78 81 11 66 72 78 81 12 66 72 78 81 12 67 72 79 82 14 67 73 79 82 15 67 73 79 82 16 68 73 80 83 17 68 74 80 83 18 69 74 81 83 19 69 75 81 84 There was a strong positive correlation between SBP and DBP with height and weight in both sexes. Concerning boys, the coefficients of correlation of SBP and DBP with height were 0.98 (p<0.001) and 0.98 (p<0.001) and with weight 0.98 (p<0.001) and 0.95 (p<0.001), respectively, while in girls the coefficients of correlation of SBP and DBP with height were 0.99 (p<0.001) and 0.98 (p<0.001) and with weight 0.97 (p<0.001) and 0.91 (p<0.001), respectively. The 90th percentile of SBP and DBP in the present study was compared for each age with the results reported in the study of the Second Task Force and Turkish children13 and the Report of the Second Task Force on Blood Pressure Control in Children in the USA.14 In boys, the 90th percentiles of SBP in Egyptian children were higher than in American children in the first 13 years, then became lower. Turkish children were higher than Egyptian children from 3 to 11 years, then became lower. The 90th percentiles for SBP in Egyptian girls were higher than in both American and Turkish girls of all ages. The DBP of American boys was lower than the Egyptians until the age of 16 years, then the DBP became higher, while the DBP of Turkish boys was lower than the Egyptians until the age of 5 years, then disclosed a significant rise. Both American and Turkish girls showed decreased DBP when compared with Egyptian girls, but this decrease exhibited an increase at the age of 12 years in American girls and at the age of 8 years in Turkish girls (figures 9–12). Figure 9 Comparison of the 90th percentile of systolic blood pressure (SBP) measurements of Egyptian (EGY) boys with the values of American (USA) and Turkish (TUR) boys. Figure 10 Comparison of the 90th percentile of systolic blood pressure (SBP) measurements of Egyptian (EGY) girls with the values of American (USA) and Turkish (TUR) girls. Figure 11 Comparison of the 90th percentile of diastolic blood pressure (DBP) measurements of Egyptian (EGY) boys with the values of American (USA) and Turkish (TUR) boys. Figure 12 Comparison of the 90th percentile of diastolic blood pressure (DBP) measurements of Egyptian (EGY) girls with the values of American (USA) and Turkish (TUR) girls. Discussion Hypertension is considered one of the main health problems all over the world due to its high occurrence and its causal relationship with morbidity and mortality.15 Estimation of BP in children and adolescents should be a chief vital domain of global healthcare. BP in children varied with age and physical mass. BP values should be matched with normal percentile curves before concluding its normality. The distribution of BP levels and the frequency of hypertension vary in different ethnic groups.10 11 The inclusion of BP measurements in the regular paediatric examination besides the establishment of national norms in children promotes discovery of asymptomatic hypertension attributable to previously unidentified disorders. In addition, it confirms that a slight increase in BP during childhood more commonly occurred than previously realised, predominantly in adolescents.16 The present study demonstrates that BP increases with age; however, this increase varied with different ages and from boys to girls, especially with the onset of adolescence. These findings are consistent with previous population-based studies.17 18 There was a sharp increase in DBP from 17 to 19 years, which may be attributed to the stress frequently encountered during the period. The rise in BP with increasing age is most probably caused by the growth of the child. Many researchers believed that the most powerful determinants of normal BP are chronological age and body size influenced by height and weight.19 Research and challenges to appreciating the field of racial variations and discrepancies in the circulatory standards are encumbered by the little knowledge foundation. In the present study, the trend of SBP and DBP rise was different from that reported by the Turkish and American Second Task Force. The latter Task Force values were based on nine different populations, including African and Mexican Americans. They used the first BP reading and not the average of two readings as in our study. Because of the absence of local data, reference standards based on American children have often been used worldwide and in most low-income and middle-income countries including Egypt. Hence, the standardisation of the American norms can be misleading and BP measurements in children should be compared with local normative BP data. Comparing BP values of African–Americans (AAs) with those of European descent, or European Americans, AAs have higher nocturnal BP as well as a smaller difference between daytime pressures. Also the lack of night-time drop in BP seen in AAs at a very young age and that accelerates during the adolescent years may be associated with increased vascular disease frequencies in AAs and may explain the frequent early onset of vascular disease, which may be attributed to the interaction of multiple influences including genetics, lifestyle regimen and deprivation.20 It is hard to define and classify race and ethnicity. The racial differences in BP measurements between Egyptian children compared with Turkish and American values may be driven by multiplex determinants including gene to gene, environment to environment, and gene to environment interfaces. Environmental aspects may include stress, body weight, and sodium and potassium consumption. Among the environmental influences are social concerns that may induce stress and possibly affect many physiological operations that are difficult to be evaluated and result in many biological upsets. Other social issues such as access to insurance coverage, income, education, physical activities and other sociodemographic variables such as age, sex and weight (body mass index) may have less interaction with biology, but these factors have certain influences on the outcome.20 21 Some researchers used an automated oscillometric device as it was easy to be used in children and to overcome the possibility of human error, but unfortunately it usually overestimates the BP matched with mercury-based sphygmomanometry. The definition of normal BP values in children is based on mercury sphygmomanometry, so in our study mercury sphygmomanometer was used to estimate BP measurements.22 Clinical BP recordings lean to be greater than home recordings, known as white coat hypertension.23 To record the real BP of subjects, the measurements were obtained in the usual settings for children, such as school and home. This variation in BP observed between different countries will postulate notable new suggestion on the variability of BP thresholds for the diagnosis and monitoring of hypertension between different ethnic clusters. Until now there are no available BP nomograms for children and adolescents in Egypt. So we are one of the few academic scientific research teams to specify BP values and curves for Egyptian girls and boys. In our study, we performed multicentric representation of diverse geographical districts in Egypt with different environments (rural and urban) and large sample size, and used the standard accurate mercury sphygmomanometers for BP records in the usual settings for children, such as school and home, overcoming the phenomenon of white coat hypertension and the inclusion of neonatal age and infancy, which were considered limitations in other studies.24 However, the exclusion of obese children in this study may create some sort of a selection bias, which may be attributed to BP variations between populations. Recent recommendations on the identification of high BP in children and adolescents offer BP standards based on sex, age, weight and height. Therefore, it may be essential to consider these variables during the establishment of reference BP standards in future work. Conclusion This analysis supports the hypothesis that regional differences, eating habits, differences in morphometric characteristics and other cultural factors may account for differences in BP levels during childhood. Therefore, every population needs to apply its own norms to delineate a measured BP level in childhood. The BP measurements of Egyptian children and adolescents can be evaluated using the standards displayed in this scientific paper. Supplementary Material Reviewer comments Author's manuscript No words could express our deep feelings towards our patients and teamwork. Contributors: Idea and design: AME-S, FME-G, DMA, WMMAEF, SAAen, MAZ. Participant enrolment and data collection: ANE-B, MAE-nAEF, AAAE-M, AMK, HMR, GMB, AGS, WSG. Data interpretation: ZAO, MAS, WAB. Manuscript writing: ZAO, MAS, WAB, WMMAEF. Statistical analysis: ZAK. Manuscript revision: RSEZ, NMSAEH, BAEG. Final approval of the submitted manuscript: all authors. Funding: This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors. Competing interests: None declared. Patient consent: Not required. Ethics approval: All procedures performed in the study were approved and were in accordance with the ethical standards of Menoufia University institutional research committee. Provenance and peer review: Not commissioned; externally peer reviewed. Data sharing statement: No additional data are available. ==== Refs References 1. Ogedegbe G , Pickering T Principles and techniques of blood pressure measurement . Cardiol Clin 2010 ;28 :571 –86 . 10.1016/j.ccl.2010.07.006 20937442 2. Jackson LV , Thalange NK , Cole TJ Blood pressure centiles for Great Britain . Arch Dis Child 2007 ;92 :298 –303 . 10.1136/adc.2005.081216 16905566 3. Rosner B , Cook NR , Daniels S , et al Childhood blood pressure trends and risk factors for high blood pressure: the NHANES experience 1988-2008 . Hypertension 2013 ;62 :247 –54 . 10.1161/HYPERTENSIONAHA.111.00831 23856492 4. Barba G , Buck C , Bammann K , et al Blood pressure reference values for European non-overweight school children: the IDEFICS study . Int J Obes 2014 ;38 Suppl 2 (Suppl2 ):S48 –S56 . 10.1038/ijo.2014.135 5. Falkner B , Gidding SS , Portman R , et al Blood pressure variability and classification of prehypertension and hypertension in adolescence . Pediatrics 2008 ;122 :238 –42 . 10.1542/peds.2007-2776 18676538 6. Rosner B , Prineas RJ , Loggie JM , et al Blood pressure nomograms for children and adolescents, by height, sex, and age, in the United States . J Pediatr 1993 ;123 :871 –86 . 10.1016/S0022-3476(05)80382-8 8229519 7. Hohn AR , Dwyer KM , Dwyer JH Blood pressure in youth from four ethnic groups: the Pasadena Prevention Project . J Pediatr 1994 ;125 :368 –73 . 10.1016/S0022-3476(05)83278-0 8071743 8. Menghetti E , Virdis R , Strambi M , et al Blood pressure in childhood and adolescence: the Italian normal standards. Study Group on Hypertension' of the Italian Society of Pediatrics' . J Hypertens 1999 ;17 :1363 –72 .10526895 9. Doll S , Paccaud F , Bovet P , et al Body mass index, abdominal adiposity and blood pressure: consistency of their association across developing and developed countries . Int J Obes Relat Metab Disord 2002 ;26 :48 –57 . 10.1038/sj.ijo.0801854 11791146 10. Goonasekera CD , Dillon MJ Measurement and interpretation of blood pressure . Arch Dis Child 2000 ;82 :261 –5 . 10.1136/adc.82.3.261 10685936 11. Al Salloum AA , El Mouzan MI , Al Herbish AS , et al Blood pressure standards for Saudi children and adolescents . Ann Saudi Med 2009 ;29 :173 –8 . 10.5144/0256-4947.51787 19448364 12. Ataei N , Aghamohammadi A , Tayebi M , Yousefi E , et al Blood pressure nomograms for school children in Iran . Pediatric Nephrology 2004 ;19 :164 –8 . 10.1007/s00467-003-1275-1 14685836 13. Tümer N , Yalçinkaya F , Ince E , et al Kara N, Ozkaya N, Ensari C, Onder S. Blood pressure nomograms for children and adolescents in Turkey . PediatrNephrol 1999 ;13 :438 –43 . 14. Report of the Second Task Force on Blood Pressure Control in Children -1987. Task Force on Blood Pressure Control in Children. National Heart, Lung, and Blood Institute, Bethesda, Maryland . Pediatrics 1987 ;79 :1 –25 .3797155 15. Chow CK , Teo KK , Rangarajan S , et al Prevalence, awareness, treatment, and control of hypertension in rural and urban communities in high-, middle-, and low-income countries . JAMA 2013 ;310 :959 –68 . 10.1001/jama.2013.184182 24002282 16. Dekkers JC , Snieder H , Van Den Oord EJ , et al Moderators of blood pressure development from childhood to adulthood: a 10-year longitudinal study . J Pediatr 2002 ;141 :770 –9 . 10.1067/mpd.2002.128113 12461492 17. Ataei N , Hosseini M , Fayaz M , et al Blood pressure percentiles by age and height for children and adolescents in Tehran, Iran . J Hum Hypertens 2016 ;30 :268 –77 . 10.1038/jhh.2015.52 26016596 18. Banker A , Bell C , Gupta-Malhotra M , et al Blood pressure percentile charts to identify high or low blood pressure in children . BMC Pediatr 2016 ;16 :98 10.1186/s12887-016-0633-7 27430884 19. National High Blood Pressure Education Program Working Group on Hypertension Control in Children and Adolescents . Update on the 1987 Task Force report on high blood pressure in children and adolescents: A working group report from the National High Blood Pressure Education Program . Pediatrics 1996 ;98 :649 –58 .8885941 20. Jones DW , Hall JE Racial and ethnic differences in blood pressure: biology and sociology . Circulation 2006 ;114 :2757 –9 . 10.1161/CIRCULATIONAHA.106.668731 17179032 21. Frist WH Overcoming disparities in U.S. health care . Health Aff 2005 ;24 :445 –51 . 10.1377/hlthaff.24.2.445 22. Frese EM , Fick A , Sadowsky HS Blood pressure measurement guidelines for physical therapists . Cardiopulm Phys Ther J 2011 ;22 :5 –12 . 23. Vaindirlis I , Peppa-Patrikiou M , Dracopoulou M , et al "White coat hypertension" in adolescents: increased values of urinary cortisol and endothelin . J Pediatr 2000 ;136 :359 –64 . 10.1067/mpd.2000.103410 10700693 24. Cantinotti M , Giordano R , Scalese M , et al Strengths and limitations of current pediatric blood pressure nomograms: a global overview with a special emphasis on regional differences in neonates and infants . Hypertens Res 2015 ;38 :577 –87 . 10.1038/hr.2015.45 25876830