==== Front BMJ OpenBMJ OpenbmjopenbmjopenBMJ Open2044-6055BMJ Publishing Group BMA House, Tavistock Square, London, WC1H 9JR bmjopen-2018-02239410.1136/bmjopen-2018-022394EpidemiologyResearch15061692Prospective investigation of folic acid supplements before and during early pregnancy and paediatric and adult cancers in the Chinese children and families cohort: a pilot study in a sample of rural and urban families Linet Martha S 1Wang Linhong 2Wang Ning 2Berry Robert J 3Chao Ann 4Hao Ling 5Li Zhu 6Fang Liwen 2Yin Peng 2Potischman Nancy 7Sun Xin 8Meng Fanweng 9Yang Ruilan 10Cong Shu 2Fan Jing 2Kitahara Cari M 1Liang Xiaofeng 11Liu Fang 12Lu Xiaojun 13Lv Fan 14Mu Chunhua 15Sampson Joshua 1Tang Yongmin 16Wan Weiqing 17Wang Baohua 2Wang Hongsheng 18Zhang Leping 19Wang Yu 11 1 Division of Cancer Epidemiology and Genetics, National Cancer Institute, Bethesda, Maryland, USA 2 National Center for Chronic and Noncommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China 3 National Center on Birth Defects and Developmental Disabilities, Centers for Disease Control and Prevention, Atlanta, Georgia, USA 4 Center for Global Health, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA 5 CDC Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, USA 6 Independent Consultant, Beijing, China 7 Office of Dietary Supplements, National Institutes of Health, Bethesda, Maryland, USA 8 National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China 9 Department of Surgery, Laoting County Maternal and Child Health Hospital, Laoting, China 10 Taicang County Maternal and Child Hospital, Taicang, China 11 Chinese Center for Disease Control and Prevention, Beijing, China 12 Center of Pediatric Blood Diseases, Tianjin Hematology Institute and Hospital, Tianjin, China 13 Department of Surgery, Taicang First People’s Hospital, Taicang, China 14 Department of Pediatric Oncology, Shanghai Xinhua Hospital, Shanghai, China 15 Laoting County Hospital, Laoting, China 16 Department of Hematology, Zhejiang University School of Medicine Children’s Hospital, Hangzhou, China 17 Department of Neurosurgery, Tiantan Hospital, Beijing, China 18 Department of Hematology/Oncology, Fudan University Children’s Hospital, Shanghai, China 19 Department of Pediatric Hematology/Oncology, Peking University Renmin Hospital, Beijing, China Correspondence to Dr Martha S Linet; linetm@mail.nih.gov2018 30 7 2018 8 7 e02239414 2 2018 08 5 2018 21 6 2018 © Author(s) (or their employer(s)) 2018. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.2018This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.Objective To determine the feasibility of long-term prospective follow-up and ascertainment of cancer in offspring and mothers from the 1993–1995 Chinese Community Intervention Program that provided folic acid supplements before and during early pregnancy to reduce neural tube defects. Design Feasibility pilot study for a prospective cohort study. Setting Families residing during 2012–2013 in one rural and one urban county from 21 counties in 3 provinces in China included in the Community Intervention Program campaign. Participants The feasibility study targeted 560 families, including 280 from the rural and 280 from the urban county included in the large original study; about half of mothers in each group had taken and half had not taken folic acid supplements. Intervention The planned new study is observational. Primary and secondary outcome measures Primary: incidence of paediatric cancers in offspring; secondary: other chronic diseases in offspring and chronic diseases in mothers Results Only 3.4% of pilot study families could not be found, 3.9% had moved out of the study area and 8.8% refused to participate. Interviews were completed by 82% of mothers, 79% of fathers and 83% of offspring in the 560 families. Almost all mothers and offspring who were interviewed also participated in anthropometric measurements. We found notable urban–rural differences in sociodemographic and lifestyle characteristics of the parents, but fewer differences among the offspring. In eight catchment area hospitals, we identified a broad range of paediatric cancers diagnosed during 1994–2013, although paediatric brain tumours, lymphomas and rarer cancers were likely under-represented. Conclusions Overall, 20 years after the original Community Intervention Program, the pilot study achieved high levels of follow-up and family member interview participation, and identified substantial numbers of paediatric malignancies during 1994–2013 in catchment area hospitals. Next steps and strategies for overcoming limitations are described. folic acid supplementspediatric cancerpilot studyepidemiologyChinese Center for Disease Control and Prevention (internal funding)Cooperative Agreements from the U.S. Centers for Disease Control and PrevneitonIntramural Research Program of the National Institutes of Health, National Cancer Institute, and the U.S. Public Health Service of the Department of Health and Human Servicesspecial-featureunlocked ==== Body Strengths and limitations of this study In the pilot study, high proportions of Chinese Community Intervention Program (CIP) mothers, fathers and offspring were traced, identified and participated in interviews and anthropometric measurements. Incomplete ascertainment of incident paediatric cancers in the CIP offspring is likely in the absence of long-standing population-based cancer registries of high quality in the geographic region where the intervention was carried out. Reduction of the small proportion of the CIP pilot study subjects who could not be traced or identified, or would not participate in interviews or anthropometric measurements will be challenging, particularly for those pilot study offspring (and parents) who have moved out of the CIP catchment area. Introduction Environmental factors and gene–environment interactions during the periconceptional, prenatal and early-life periods are increasingly linked with cancer and other serious diseases during childhood, adolescence and adulthood, but research is limited and mechanisms are poorly understood.1 2 Support for the in utero origin of paediatric leukaemia, mostly acute lymphoblastic leukaemia, has been provided by observations on twins showing that the chromosomal rearrangements seen in leukaemia cases could occur in utero, coupled with work demonstrating that certain chromosomal translocations are present in the blood spot cards of children who later developed childhood acute lymphoblastic leukaemia with the same translocations.3 The only established risk factors for paediatric cancer (eg, ionising radiation, a few genetic or congenital syndromes and birth weight)4 explain <20% of occurrence.5 6 Modest increased7–10 or reduced risks11 12 have recently been linked with other exposures in pooled paediatric leukaemia case–control studies. Prospects for prevention are limited to avoidance of ionising radiation or high birth weight, although more recently periconceptional folic acid (FA) supplements have been linked with reduced risks in a large pooled analysis.11 However, results for the association of periconceptional or prenatal FA supplements and paediatric leukaemia have been inconsistent in individual studies, and these inconsistencies and the modest associations observed in the pooled analysis may be due in part to differential or inaccurate long-term recall,13 14 selection bias15 and declining participation rates, and growing socioeconomic differences between control and case subjects.16–18 Prospective large epidemiological studies could overcome many of these limitations. A unique opportunity to evaluate the potential role of FA supplements before and during early pregnancy in reducing risk of paediatric leukaemia in offspring was identified 20 years after a 1993–1995 Community Intervention Program (CIP) in which FA supplements before and during early pregnancy were provided in 21 Chinese counties to reduce neural tube defects (NTD). Among offspring of 130 142 women who took 400 µg FA supplements compared with offspring of the 117 689 women who did not take FA supplements, there was a 79% and 41% reduction in the prevalence of NTD in high (northeast) and lower (southeast) rate regions, respectively.19 Extensive information had been collected during 1993–1995 on maternal sociodemographic, lifestyle, reproductive, medical conditions and treatments, occupational and other factors during the periconceptional and prenatal periods, and on offspring during the early neonatal period. We conducted a pilot study to assess the feasibility of: (1) establishing a large cohort (designated the Children and Families Cohort Study (CFCS)) from the CIP families for planned prospective epidemiological studies evaluating paediatric leukaemia and other health outcomes in offspring and mothers; (2) collecting data from two generations of family members by interview and anthropometric measurements; and (3) identifying and validating paediatric cancers diagnosed during 1994–2013 in the CIP catchment area among children who are potentially eligible CIP offspring. Methods Study team organisation and approvals In 2012–2013, investigators from the Chinese Center for Disease Control and Prevention (China CDC), US Centers for Disease Control and Prevention (US CDC), the US National Cancer Institute (NCI), the Maternal and Child Health (MCH) Hospital leaders of each county and clinicians of the hospitals selected for identification of paediatric cancers conducted data collection. The protocol was approved by the Chinese provincial, city, and county health bureaus, county MCH hospitals and other participating hospitals, and by the Chinese and US CDCs and US NCI ethics review committees (see details in Ethics Approval section following the Discussion). Study sample Five hundred CIP families were targeted from two CIP geographic regions, for example, a rural county in the northeast and an urban county in the southeast. From the pregnancy-monitoring registration system, 280 families from each county were selected with eligibility criteria including: (1) mother resided and underwent a premarital or prenatal physical examination in the specified county during 1993–1995; (2) the pregnancy resulted in a live, single birth in the county by December 1996; (3) sex of the infant was known and (4) high-quality data on maternal use of FA supplements before and during the pregnancy were available. Tracing and contacting families County MCH staff verified the names, vital status, current addresses and phone numbers of eligible mothers and children selected by comparing the lists with township and village health service and New Rural Cooperative Insurance rosters. To improve recruitment, the county-level MCH, township hospital, village or community health service centre physicians, assisted by the village development and community/street committee staff, made initial contact with the families. A letter describing the study purpose, questionnaire topics, physical measurements, confidentiality, benefits, potential risks, incentives and opportunity to opt out of all or part of the data collection was mailed or delivered in person. After letter delivery, the village or Township doctor contacted the mother/caretaker to answer questions and arranged a home visit where consent was obtained. Interviewer training After a didactic 2-day training, interviewers practised questionnaire administration and taking anthropometric measurements. The training concluded with instructions on manual review and data editing. Approximately 1 month later, supervisory Chinese CDC staff again evaluated staff and retrained as needed. Data collection: questionnaires One of two maternal questionnaires assessed family residential characteristics and maternal sociodemographic characteristics, lifestyle, reproductive and medical history, and job history. If the child resided with a caretaker, a modified shorter questionnaire was administered. A second maternal/caretaker questionnaire asked about the offspring’s date of birth, vital status, history of cancer or birth defects, diagnosis of autism, developmental milestones, highest level of education and job history. The offspring’s questionnaire asked date of birth, self-rating of physical health, source of medical care, number of non-routine health visits in the past 12 months, cigarette smoking and secondhand smoke history, use of alcohol and history of pubertal changes. The father’s questionnaire inquired about date of birth, education, marital status, job history, smoking and secondhand smoking, alcohol use and medical history. Other data collection (results reported separately) After the interviews, offspring and mothers were invited to participate in anthropometric measurements at the Township hospitals. At the end of questionnaire and anthropometric measurements data collection, the participants received appreciation certificates and incentives. A letter was also provided indicating that the participant might be contacted for a second study to assess diet, nutrition, physical activity and ultraviolet radiation exposure. Identification and validation of paediatric cancer cases In consultation with hospitals and community leaders, eight hospitals providing different levels of services were selected from study regions and major cities near to the catchment area where CIP children might have been referred for diagnosis and treatment of paediatric cancer. A workshop was held with experts in paediatric haematology, oncology and neurosurgery from these hospitals to develop the methods for identification and validation of the paediatric cancer outcomes. The medical record review process included: (1) searching for medical records to identify all paediatric patients with cancer born during 1994–1996 who were diagnosed and/or treated during 1994–2013; (2) developing a list of all potentially eligible patients (born during 1994–1996 in the 21 counties included in the CIP or, if no specific place of birth was listed, those born in the three provinces in which the 21 counties were geographically located); (3) obtaining and abstracting medical records of these patients; and (4) independent reviewing of the abstract forms by two clinical experts with resolution of differences through discussion to determine a final diagnosis. For quality control purposes, each hospital was visited at least once by China CDC staff to evaluate procedures. In the absence of re-identification of the entire CIP cohort and comprehensive population-based cancer registries, it was not possible to link the paediatric cancer cases identified in hospitals with the cohort. Data management A data management training workshop was held in Beijing for China CDC staff followed by a detailed review and editing of the data collected. All forms were coded, checked, entered twice and data verified for accuracy. Range and logic sequences were verified against original paper forms and corrections made as required. Statistical analysis In data analysis conducted during 2016–2017, cross-tabulation was used to evaluate the distribution of demographic, socioeconomic, lifestyle, developmental and medical history characteristics by county. Fisher’s exact tests were used to test for urban versus rural geographic differences by covariates among mothers, offspring and fathers. Subjects and public involvement Subjects and the public were not involved in development of the research question, the study design, determination of the outcome measures to be studied, recruitment or conduct of the study. Subjects were informed about results of anthropometric measures and blood chemistry laboratory tests (methods for the laboratory test components of a second pilot study are reported elsewhere). Subjects also received physical activity monitors at the end of the physical activity monitoring component of the second pilot study (methods reported elsewhere). If the full-scale study is funded, the findings would be shared with the subjects, public health officials and medical professionals through newsletters and other types of communication. Results Of the 560 families selected for the pilot study, only 3% (2% in the rural and 5% in the urban centre) could not be found, 4% of families reportedly moved away (6% in the rural and 2% in the urban centre) and 9% (8% in the rural and 9% in the urban centre) refused to be contacted (table 1). Overall, 82% of mothers, 79% of fathers and 83% of offspring completed interviews of the 560 families selected. Among the 469 families (84% of the 560 selected) targeted for interviews (after excluding those who could not be found, had moved away or had refused to be contacted), the interview participation was very high, for example, 98% of mothers, 95% of fathers and 99% of offspring. Almost all mothers and offspring who were interviewed also participated in the anthropometric measurements (table 1). The proportion of mothers contacted who took FA supplements (49% in the rural centre and 52% in the urban centre) was similar to the 50% in each centre who took FA in the samples originally selected. Table 1 Population targeted for pilot study, description of participants, reasons for non-participation, and residential and sociodemographic characteristics of participating families Rural area N (%) Urban area N (%) Both areas N (%) P value* Population targeted, description of participants and reasons for non-participation in pilot study Number of families selected for pilot 280 280 560 Families selected but not included 46 (16.4%) 45 (16.1%) 91 (16.3%) Reason not included  Moved away 17 (6.1%) 5 (1.8%) 22 (3.9%)  Not found 6 (2.1%) 13 (4.6%) 19 (3.4%)  Refused 23 (8.2%) 26 (9.3%) 49 (8.8%)  Mother deceased 0 1 (0.3%) 1 (0.1%) Families targeted for interviews† 234 (83.6%) 235 (83.9%) 469 (83.8%) Mothers who consented among families targeted for interview 229 (97.9%) 231 (98.3%) 460 (98.1%) Mothers  Completed interview‡ 229 (81.8%) 231 (82.5%) 460 (92.1%)  Physical measurements§ 228 (99.6%) 230 (99.6%) 458 (99.6%) Fathers  Completed interview‡ 232 (82.9%) 212 (75.7%) 444 (79.3%) Children  Completed interview‡ 233 (83.2%) 229 (81.8%) 462 (82.5%)  Physical measurements§ 231 (99%) 229 (100%) 460 (99.6%) Caretakers  Completed interview¶ 6 9 15 Residential and sociodemographic characteristics of participating families Number of years at current home  ≤10 23 (10%) 111 (48%) 134 (29%) <0.0001  11–19 138 (59%) 76 (33%) 214 (46%)  20+ 71 (31%) 45 (19%) 116 (25%)  Unknown 0 1 (0.4%) 1 (0.2%) Own or rent home  Own 224 (97%) 216 (93%) 440 (95%) 0.0572  Rent 7 (3%) 17 (7%) 24 (5%)  Unknown 1 (0.4%) 0 1 (0.2%) Highest number of persons residing in residence in the past year  ≤3 100 (43%) 97 (42%) 197 (42%) <0.0001  4 83 (36%) 26 (11%) 109 (23%)  ≥5 49 (21%) 110 (47%) 159 (34%) *Fisher’s exact test for rural versus urban differences. †Targeted for interviews were those who had not moved away, could be found, did not refuse or mother not deceased. ‡Per cents shown are the proportion completing interviews of those selected for the pilot study. §Per cents are the proportions who completed physical measurements of those who were interviewed. ¶If mother not available, caretaker information was used. Nearly half of urban families resided 10 or fewer years in their current home, whereas 90% of rural families had lived >10 years in the current home (table 1). More than 93% of rural and urban families owned their current home. Close to half of the urban families compared with 21% of rural families had five or more persons recently residing in their residence. Urban mothers were younger at the age of marriage registration, birth of the CIP offspring, and at the age of current interview completion (table 2). Rural mothers reported less education, held fewer jobs and a slightly higher proportion were married compared with urban mothers. Almost none of the rural and urban mothers reported smoking cigarettes. Rural mothers (43%) were somewhat more likely than urban mothers (33%) to report being exposed to secondhand smoke during their pregnancies for ≥3 days per week. Rural mothers (45%) were more likely than urban mothers (35%) to report that their offspring had been exposed to secondhand smoke ≥ 3 days per week before age 5 years. Few of the mothers (5% overall, 8% urban versus 1% rural) reported drinking alcohol. Rural mothers were more likely to report onset of menstrual periods at older ages, and 30% of rural compared with 16% of urban mothers reported only one pregnancy. Table 2 Maternal sociodemographic, lifestyle and reproductive characteristics, Chinese CFCS questionnaire, 2013 Characteristics Rural area n=229 (%) Urban area n=231 (%) Both areas n=460 (%) P value* Age at CIP enrolment/marriage registration  ≤22 84 (37%) 126 (55%) 210 (46%) 0.0004  23–24 66 (29%) 59 (26%) 125 (27%)  ≥25 79 (34%) 46 (20%) 125 (27%) Age at first birth  ≤23 88 (38%) 132 (57%) 220 (48%) <0.0001  24–25 58 (25%) 57 (25%) 115 (25%)  ≥26 83 (36%) 42 (18%) 125 (27%) Age at CFCS interview completion  ≤39 70 (31%) 116 (50%) 186 (40%) <0.0001  40–41 72 (31%) 67 (29%) 139 (30%)  ≥42 87 (38%) 48 (21%) 135 (29%) Highest level of education  None 3 (1%) 1 (0.4%) 4 (1%) <0.0001  Some or all elementary school 42 (18%) 17 (7%) 59 (13%)  Completed middle school 144 (63%) 143 (62%) 287 (62%)  Completed high school/technical school 39 (17%) 46 (20%) 85 (18%)  Completed some or all college/university 1 (0.4%) 24 (10%) 25 (5%) Current marital status  Married 227 (99%) 216 (94%) 443 (96%) 0.0002  Widowed 2 (1%) 1 (0.4%) 3 (1%)  Divorced 0 12 (5%) 12 (3%)  Other 0 2 (1%) 2 (0.4%) Current number of jobs after completed school  None 0 1 (0.4%) 1 (0.2%) <0.0001  1 158 (69%) 21 (9%) 179 (39%)  2 48 (21%) 68 (29%) 116 (25%)  3+ 23 (10%) 141 (61%) 164 (36%) Smoking/secondhand smoke  Never smoked cigarettes regularly 227 (99%) 230 (100%) 457 (99%) 0.6225  Exposed to secondhand smoke at home >10 years before age 20 91 (40%) 79 (34%) 170 (37%) 0.2872  Exposed to secondhand smoke at home >10 years after age 20 125 (55%) 122 (53%) 247 (54%) 0.5299  Exposed to secondhand smoke during CIP pregnancy for three or more days per week 99 (43%) 77 (33%) 176 (38%) 0.0689 Child exposure to secondhand smoke before age 5 years  None 101 (44%) 105 (45%) 206 (45%) 0.0088  <3 days per week 22 (10%) 43 (19%) 65 (14%)  3+days per week 103 (45%) 80 (35%) 183 (40%)  Unknown 3 (1%) 3 (1%) 6 (1%) Ever drink alcohol  No/less than once per  month 226 (99%) 213 (92%) 439 (95%) 0.0012  Yes 3 (1%) 18 (8%) 21 (5%) Age menstrual periods started, years  12–13 29 (13%) 47 (20%) 76 (17%) 0.0497  14–15 120 (52%) 122 (53%) 242 (53%)  16+ 78 (34%) 62 (27%) 140 (30%)  Unknown 2 (1%) 0 2 (0.4%) Number of pregnancies  1 68 (30%) 37 (16%) 105 (23%) 0.0002  2 95 (41%) 86 (37%) 181 (39%)  3 53 (23%) 74 (32%) 127 (28%)  4+ 13 (6%) 34 (15%) 47 (10%) *Fisher’s exact test for rural versus urban differences. CFCS, Children and Families Cohort Study; CIP, Community Intervention Program. Rural fathers were older at the time of marriage registration, birth of their offspring and at the current interview compared with urban fathers (table 3). Rural fathers reported less education and fewer jobs compared with urban fathers. A higher proportion of rural (33%) compared with urban (19%) fathers reported never smoking, although rural fathers were more likely than urban fathers to be exposed to secondhand smoke for over 10 years before age 20. Urban (75%) compared with rural (60%) fathers were more likely to be currently smoking. Use of alcohol was similar between rural (63%) and urban (65%) fathers, but a somewhat higher proportion of urban (61%) than rural (57%) fathers reported alcohol use for ≥15 years. Table 3 Paternal sociodemographic and lifestyle characteristics, Chinese CFCS questionnaire, 2013 Characteristics Rural area n=232 (%) Urban area n=212 (%) Both areas n=444 (%) P value* Age at CIP enrolment/marriage registration  ≤22 77 (33%) 85 (40%) 162 (36%) 0.0030  23–24 56 (24%) 69 (33%) 125 (28%)  ≥25 99 (43%) 58 (27%) 157 (35%) Age at birth of child  ≤23 71 (31%) 91 (43%) 162 (36%) 0.0002  24–25 59 (25%) 67 (32%) 126 (28%)  ≥26 102 (44%) 54 (25%) 156 (35%) Age at CFCS interview completion  ≤39 58 (25%) 77 (36%) 135 (30%) 0.0038  40–41 68 (29%) 69 (33%) 137 (31%)  ≥42 106 (46%) 66 (31%) 172 (39%) Highest level of education  None 1 (0.4%) 0 1 (0.2%) <0.0001  Some or all elementary school 43 (19%) 16 (8%) 59 (13%)  Completed middle school 153 (66%) 125 (59%) 278 (63%)  Completed high school/technical school 32 (14%) 49 (23%) 81 (18%)  Completed some or all college/university 3 (1%) 22 (10%) 25 (6%) Number of jobs after completed school  1 146 (63%) 28 (13%) 174 (39%) <0.0001  2 63 (27%) 65 (31%) 128 (29%)  3+ 23 (10%) 119 (56%) 142 (32%) Currently smoking cigarettes  Never smoked 77 (33%) 40 (19%) 117 (26%) 0.0008  No 16 (7%) 13 (6%) 29 (7%)  Yes 139 (60%) 159 (75%) 298 (67%) Secondhand smoke  Exposed to secondhand smoke at home >10 years before age 20 103 (44%) 73 (34%) 176 (40%) 0.0275  Exposed to secondhand smoke at home >10 years after age 20 53 (23%) 56 (26%) 109 (25%) 0.7922 Use of alcohol  No/less than once per month 86 (37%) 74 (35%) 160 (36%) 0.6924  Yes 146 (63%) 138 (65%) 284 (64%) Use of alcohol in years  Never 86 (37%) 74 (35%) 160 (36%) <0.0001  <15 1 (0.4%) 2 (1%) 3 (1%)  15–19 8 (3%) 36 (17%) 44 (10%)  20+ 125 (54%) 94 (44%) 219 (49%)  Unknown 12 (5%) 6 (3%) 18 (4%) *Fisher’s exact test for rural versus urban differences. CFCS, Children and Families Cohort Study; CIP, Community Intervention Program. Urban versus rural differences in sociodemographic factors were less apparent among offspring (table 4). The two groups were similar in birth year, age at interview, and sex distribution, but somewhat more urban than rural offspring reported living with their parents. Most in each group were full-time students, but urban offspring were more likely to report attending trade or technical school. Most offspring in both groups reported never smoking cigarettes regularly; a somewhat higher proportion of urban than rural offspring reported secondhand smoke exposure at home for ≥1 days per week and alcohol use for ≥1 days since birth. Table 4 Offspring sociodemographic and lifestyle characteristics, Chinese CFCS questionnaire, 2013 Characteristics Rural area n=233 (%) Urban area n=229 (%) Both areas n=462 (%) P value* Year of birth  1994 67 (29%) 70 (31%) 137 (30%) 0.7667  1995 106 (45%) 96 (42%) 202 (44%)  1996 60 (26%) 62 (27%) 122 (26%)  Unknown 0 1 (0.4%) 1 (0.2%) Age at interview completion, years  <16 88 (38%) 82 (36%) 170 (37%) 0.5264  16 93 (40%) 102 (45%) 195 (42%)  17+ 52 (22%) 44 (19%) 96 (21%)  Unknown 0 1 (0.4%) 1 (0.2%) Sex  Female 120 (52%) 112 (49%) 232 (50%) 0.6418  Male 113 (48%) 117 (51%) 230 (50%) Living arrangements  Living with parents 211 (91%) 221 (97%) 432 (94%) 0.0001  Living with grandparents or other adult guardian 1 (0.4%) 3 (1%) 4 (1%)  Other 15 (6%) 0 15 (3%)  Unknown 6 (2.6%) 5 (2.2%) 11 (2.4%) Current activity: school, work, other  Full-time student 215 (92%) 208 (91%) 423 (92%) 0.2205  Working part-time and attending school part-time 0 3 (1%) 3 (1%)  Working 6 (3%) 3 (1%) 9 (2%)  Not working and not attending school 7 (3%) 10 (4%) 17 (3.7%)  Unknown 5 (2%) 5 (2.4%) 10 (2.2%) Highest level of education  Junior high school 43 (18%) 18 (8%) 61 (13%) <0.0001  Senior high school 123 (53%) 94 (41%) 217 (47%)  Trade or technical school 49 (21%) 99 (43%) 148 (32%)  Unknown 18 (8%) 18 (8%) 36 (8%) Ever smoked cigarettes regularly  No 223 (96%) 215 (94%) 438 (95%) 0.4085  Yes 10 (4%) 14 (6%) 24 (5%) Number of days exposed to secondhand smoke where you live in past week  0 158 (68%) 143 (62%) 301 (65%) 0.0269  1–4 33 (14%) 55 (24%) 88 (19%)  5+ 38 (16%) 30 (13%) 68 (15%)  Unknown 4 (2%) 1 (0.4%) 5 (1%) Number of days ever drank alcohol since birth  Never 199 (85%) 133 (58%) 332 (72%) <0.0001  1–2 14 (6%) 40 (17%) 54 (12%)  3–9 11 (5%) 29 (13%) 40 (9%)  10–19 2 (1%) 10 (4%) 12 (3%)  ≥20 5 (2%) 13 (6%) 18 (4%)  Unknown 2 (1%) 4 (2%) 6 (1%) *Fisher’s exact test for rural versus urban differences. CFCS, Children and Families Cohort Study. Eight hospitals selected included county and provincial hospitals, children’s medical centres and referral hospitals in the nearby cities of Beijing (treating brain tumours), Tianjin (haematological disorders) and Shanghai (paediatric oncology) to which children would have been referred for definitive diagnosis and treatment of paediatric cancer (table 5). A broad range of incident paediatric malignancies and related disorders were identified in the eight hospitals; of the total cancers, 77% were designated by type and 23% were unspecified (table 4). Of the 137 paediatric cancers identified, 37% were leukaemias. Only 10% (13/137) of the paediatric neoplasms were brain tumours and 7% (9/137) were lymphomas, although similar numbers were identified in the earlier (1994–2003) and later (2004–2013) time periods. No retinoblastomas were identified. Table 5 Paediatric cancers diagnosed 1994–2013* among children residing at birth in the CFCS catchment area Types of paediatric cancers Number of cases diagnosed 1994–2003 Number of cases diagnosed 2004–2013 Total (%) Leukaemia 16 34 50 (36.5%)   Acute lymphoblastic leukaemia 13 18 31   Acute myeloid leukaemia 3 13 16   Myelodysplastic syndrome 0 2 2   Other types of leukaemia 0 1 1 Lymphoma 5 4 9 (6.6%)   Hodgkin lymphoma 1 1 2   non-Hodgkin’s lymphoma 3 3 6   Other lymphoma 1 0 1 Brain tumours 7 6 13 (9.5%)   Astrocytoma 3 3 6   Medulloblastoma 0 0 0   Primitive neuroectodermal tumour 0 0 0   Other brain tumours 4 3 7 Retinoblastoma 0 0 0 Renal Tumours 8 1 9 (6.6%)   Wilms’ tumours 7 1 8   Other renal tumours 1 0 1 Hepatic tumours 3 1 4 (2.9%)   Hepatoblastoma 3 0 3   Other hepatic tumours 0 1 1 Malignant bone tumours 1 2 3 (2.2%)   Osteosarcomas 1 1 2   Other malignant bone tumours 0 1 1 Soft tissue sarcomas 2 1 3 (2.2%)   Rhabdomyosarcoma 2 0 2   Other soft tissue sarcomas 0 1 1 Germ cell tumours 2 10 12 (8.8%)   Testicular cancer 1 0 1   Ovarian tumours 0 4 4   Other germ cell tumours 1 6 7 Carcinomas 1 2 3 (2.2%)   Thyroid cancer 1 2 3   Melanoma 0 0 0   Other types of carcinoma 0 0 0 Unspecified cancers 15 16 31 (22.6%) Total cancers 60 77 137 Other related conditions   Aplastic anaemia 12 26 38   Fanconi anaemia 0 1 1 *Diagnosed in eight hospitals. CFCS, Chinese Children and Families Cohort Study. Discussion In the pilot study of 580 CIP families, we located all but 3.4% of the families and only 8.7% refused to participate. Overall, 82% of mothers, 79% of fathers and 83% of offspring selected of the 560 families chosen completed interviews. More than 90% of those who agreed to be contacted completed interviews, and 99% of the mothers and offspring completed anthropometric measurements. We found notable differences in sociodemographic and lifestyle characteristics between rural and urban parents, but fewer differences in offspring. We identified a broad range of incident (1994–2013) paediatric cancers in the diverse hospitals, but there appeared to be incomplete ascertainment of paediatric brain tumours, lymphomas and certain others (retinoblastoma), and 23% were of unspecified subtype. Supporting our findings of notable rural versus urban differences in parents, data since the 1990s from the National Bureau of Statistics of China have consistently shown significant differences between rural and urban areas in gross domestic product (GDP), per capita income and educational attainment.20 The two pilot study centres differed significantly in geography and urbanicity (rural northeast vs urban southeast regions), GDP (higher in southeast), principal industry (agricultural in the northeast vs high technology industry in the southeast) and greater in-migration in the southeast. Recently, household income, education and health status have been shown to account for the lower use of preventive care services by rural compared with urban residents.21 We also note significant differences in reported parity and gravidity, consistent with data reported at CIP enrolment in 1993–1995. The lack of urban–rural differences reported by offspring may reflect adolescent lifestyle changes over time, as illustrated by a recent report on obesity among children in Shandong Province, China.22 The higher urban compared with rural prevalence of childhood overweight and obesity in Shandong during 1985–2005 no longer existed in 2014 due to the increasing prevalence of overweight and obesity among rural children over time, a change attributed to economic development and lifestyle changes in rural areas. Strengths and limitations Compared with many cohort studies, the proportion of families refusing to participate was relatively small, but strategies should be developed to further minimise refusals and entice higher participation. New strategies are needed to locate offspring who are increasingly likely to move away from their childhood residences for educational or employment opportunities. The ideal approach for identifying incident paediatric cancers in the Chinese Children and Families Cohort Study would have been linkage with population-based cancer registries, but there have been no long-standing population-based cancer registries of high quality in the geographic regions where the original CIP was carried out. Although the pilot study included different types of hospitals where children with paediatric cancer might be initially seen and treated, the likely incomplete ascertainment of certain cancer outcomes, a known problem described in compendia of international population-based cancer registry data,23 suggests the need for learning more about referral patterns for paediatric cancers. Focus groups of hospital and medical professional society leaders would be useful to discuss strategies for improving identification of paediatric cancer cases, particularly those diagnosed in earlier time periods. In the absence of comprehensive coverage of the geographic catchment area by high-quality population-based cancer registries, it is difficult to assess the likely success of matching cohort members with the paediatric cancer cases identified through the hospital component of the pilot study. A more expensive strategy would be to add active follow-up all offspring and parents to identify and verify incident paediatric cancers and to review death certificates to confirm those reported as deceased. In the absence of death certificates, particularly in the early time periods, verbal autopsy interviews may be needed. Overall, the pilot study revealed positive results for re-identifying, tracing, interviewing and obtaining anthropometric measurements in the original CIP families to establish the new CFCS cohort. A larger feasibility study over a broader geographic region is needed to verify that the strategies used in the current pilot study would continue to yield success in tracing, locating and obtaining the high participation levels needed for cohort follow-up. The large number of paediatric cancers identified is also promising, but further work is needed to improve the level of ascertainment, and test the feasibility of matching paediatric cancers to the CIP children. If a larger feasibility study is successful, we propose to carry out a prospective record linkage CFCS to compare risk of paediatric leukaemia, brain tumours and other paediatric cancers in offspring of 130 142 Chinese mothers who took vs offspring of 117 689 mothers who did not take FA supplements before and during early pregnancy in the 1993–1995 CIP to complement case–control study findings of reduced risks of childhood leukaemia associated with mothers’ periconceptional use of FA. In addition, the new CFCS cohort would be a valuable scientific resource that could evaluate a wide range of exposures and outcomes, and address many important research questions once assembled. Supplementary Material Reviewer comments Author's manuscript The authors are indebted to the county and township-level Maternal and Child Health hospitals nurses and physicians who interviewed the family members; the Maternal Child Health township hospitals, village and community development and community/street committee staff who made the initial contact with the selected families; the hospital and community leaders who identified the eight hospitals included in the pilot study; and the hospital directors, department chairs, senior haematologists, oncologists, neurosurgeons and numerous other medical staff who supported the hospital component with identification and abstraction of medical records of incident pediatric cancer cases. They also thank the families who participated in the feasibility study. They express appreciation to IMS, Inc. computer programmers for preparation of data files and computing support. MSL and LW contributed equally. Contributors: MSL, LW, RJB, AC, LH, LF, ZL, NP, CMK, XL, JS and YW: study concept and design, oversight of all aspects of the study, reviewed data and wrote manuscript. NW, PY and XS: overall supervision of field work and training, and development of operational field manuals. FM: supervised field work and training of interviewers in rural country. RY: supervised field work and training of interviewers in urban county. NW, LF, PY and BW: conducted quality control supervisory visits and other quality control efforts; JF, RJB, AC, LF, MSL and NP: oversaw data entry, manual and electronic data review, and conducted data analysis. LH, LF, SC, MSL and RJB: responsible for financial supervision. FL, XL, FLv, CM, YT, WW, HW and LZ: provided key assistance with access and collaboration of eight hospitals, developed and reviewed the field manual for identifying eligible paediatric cancer cases, oversaw efforts to identify eligible pediatric cancer cases, oversaw abstracting of medical records, reviewed medical record abstracts and determined final diagnoses of paediatric cancer cases. Funding: All funding for this project was provided by the Intramural Research Program of the National Institutes of Health, National Cancer Institute, and the US Public Health Service of the Department of Health and Human Services; cooperative agreements from the US Centers for Disease Control and Prevention (U01 GH0000029 and 5U 19 GH000636); and internal funding from the Chinese Center for Disease Control and Prevention. Competing interests: None declared. Ethics approval: The protocol was approved by the institutional ethics review committees including: the National Cancer Institute Special Studies Institutional Review Board (NCI SSIRB - 11CN165 for original and annual renewals); the US Centers for Disease Control and Prevention, which relied on the NCI SSIRB original and annual IRB approvals (CDC protocol 6140); and the Chinese Center for Disease Control and Prevention (CCDC protocol 201110 approved August 8, 2011). Informed written consent was obtained for all aspects of data collection (interviews and anthropometric measurements) from all subjects aged ≥18. Written assent was obtained from children under age 18. The participants all received certificates of appreciation; mothers received vouchers for purchasing groceries (US$ value = $15); children received memory sticks (US$ value = $7.60. Identification of pediatric cancers diagnosed during 1994-2013 in the catchment area of the original CIP study was carried out within each hospital and data collected were stripped of all personal identifying information before being provided to the Chinese CDC and other collaborators. Provenance and peer review: Not commissioned; externally peer reviewed. Data sharing statement: The study protocol, data collection instruments, field and coding manuals are available from the corresponding author upon request. If the full-scale study is funded, the findings would be shared with the subjects, public health officials and medical professionals through newsletters and other types of communication. The main results of the study will also be reported in peer-reviewed scientific journals, and at professional society meetings and international conferences. 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