==== Front JAAD Int JAAD Int JAAD International 2666-3287 Elsevier S2666-3287(23)00073-1 10.1016/j.jdin.2023.04.011 Research Letter Evaluation of hair regrowth after minoxidil and dutasteride tattooing in men with androgenetic alopecia Ragi Sara D. MS a Ghanian Soha MD a Rogers Nicole MD b Peterson Danielle M. MD c Johnson Luke S. MD d Wambier Carlos G. MD, PhD carlos_wambier@brown.edu @WambierMD a∗ a Department of Dermatology, The Warren Alpert Medical School of Brown University, Rhode Island Hospital, Providence, Rhode Island b Hair Restoration of the South, Metairie, Louisiana c Department of Dermatology, Yale School of Medicine, New Haven, Connecticut d Department of Dermatology, University of Utah, Salt Lake City, Utah ∗ Correspondence to: Carlos Gustavo Wambier, MD, PhD, Department of Dermatology, The Warren Alpert Medical School of Brown University, Rhode Island Hospital, 593 Eddy Street, Jane Brown Building, 1st floor, Room 115, Providence, RI 02903 carlos_wambier@brown.edu@WambierMD 20 5 2023 9 2023 20 5 2023 12 103104 © 2023 by the American Academy of Dermatology, Inc. Published by Elsevier Inc. 2023 American Academy of Dermatology, Inc. https://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Key words alopecia androgenetic alopecia antiandrogens baldness drug delivery dutasteride hair loss male pattern hair loss microneedling minoxidil minoxidil sulfate tattoo machine ==== Body pmcTo the Editor: Topical minoxidil and oral 5-α-reductase inhibitors have been the usual care for male androgenetic alopecia (AGA) for decades. Such drugs have been adopted through intralesional injections for the treatment of AGA.1,2 Drug delivery with tattoo equipment3,4 has also been adopted with such medications to the balding scalp.5 The aim of this study was to evaluate the clinical outcome of 3 monthly sessions of minoxidil-dutasteride tattooing (MDT). We conducted a retrospective analysis of patients who underwent MDT from 2016 to 2020 because of a lack of response to topical minoxidil and oral 5-α-reductase inhibitors for >6 months. We included all male patients who had received 3 monthly sessions. Out of 73 patients with at least 1 session, a total of 15 men were included. Deidentified pooled photographs (baseline and 4 months of follow-up) were randomized for top-quadrant Severity of Alopecia Tool (SALT) blinded scoring by 3 evaluators via an online survey. The primary outcome was defined as achieving >10% top scalp area regrowth (TSAR), calculated based on the difference in the top-quadrant SALT scores. The secondary outcomes included response rate per presence of vellus hair and scalp photoaging at the baseline. The patients gave consent for their photographs and medical information to be published in print and online and with the understanding that this information may be publicly available. After cleaning the scalp with 70% isopropanol, a rotatory tattoo machine (Cheyenne, MT.DERM) set at 120 Hz using a 27-needle cartridge (3240 perforations/s) at 1.5-mm needle exposure was used to deliver sterile-compounded 0.5% minoxidil sulfate (1 mL) and 0.1% dutasteride (1 mL) (Pineda). The patients were authorized to continue oral 5-α-reductase inhibitors and instructed to resume topical minoxidil after a week after the procedure. The median age was 49 years (range, 24-69 years), 9 patients (60%) exhibited signs of scalp photoaging, 8 (53%) had a high density of vellus hair, 1 (7%) previously underwent scalp microblading to conceal AGA, and 1 (7%) underwent hair transplant. The baseline median top SALT was 60% (interquartile range, 38%-78%) and 40% (interquartile range, 19%-67%) at 4 months (Wilcoxon signed-rank test, P < .001). Eight patients (53%) achieved a mean TSAR of >10%, and 4 (27%) achieved a mean TSAR of >20% (Fig 1). Overall, the median TSAR was 10% (interquartile range, 5%-20%) (Supplementary Fig 1, available via Mendeley at https://data.mendeley.com/datasets/6r865wbgc7).Fig 1 Androgenetic alopecia. Photographs with the same hair length. Illustrative standardized cross-polarized overhead images of the top quadrant utilizing Reveal Imager (Canfield Scientific) for top-quadrant Severity of Alopecia Tool scoring by evaluators. (A) Before, the top Severity of Alopecia Tool score was 53%. (B) After 4 months, the top Severity of Alopecia Tool score was 28%. A successful 25% top scalp area regrowth was observed after 3 sessions of minoxidil and dutasteride drug delivery through tattooing. In patients with vellus hair at the baseline, 63% of the evaluations achieved >10% TSAR compared with 22% in those with no vellus hair (Fisher exact test, P = .014). In patients with scalp photoaging at the baseline, 26% achieved >10% TSAR compared with 78% in those with no scalp photoaging (P = .001). All patients reported scalp desquamation for 2 to 3 days in the first week after MDT. Infection, scarring, or worsened alopecia did not develop in any patient. Procedural pain was managed with topical anesthetics or neural blocks. Most patients experienced significant hair growth, as measured based on TSAR, which was derived from the top-quadrant SALT scores using top standardized photography only (Fig 1). Histology or trichoscopy evaluations were not performed. A lack of response to MDT may be explained by a combination of factors, including low vellus hair density and scalp photoaging (such as pigmentation and solar lentigines) at the baseline. Our findings provide preliminary evidence of the use of MDT for male AGA and useful information for future prospective double-blinded, randomized, placebo-controlled trials of drug delivery. Conflicts of interest Dr Wambier has served as an adviser for Applied Biology, Chemistry Rx, Daniel Alain, and Young Pharmaceuticals and as an investigator for Concert Pharmaceuticals, Incyte, Eli Lilly, Pfizer, Sun Pharma, and UCB. Dr Peterson has served as investigator for Concert Pharmaceuticals, Incyte, Eli Lilly, Pfizer, and AnaptysBio. The other authors have no potential conflict of interest to disclose. Funding sources: None. IRB approval status: The Brown University’s IRB Human Research Protection Program approved the author’s determination that the submitted study #003208/2022 does not meet the definition of human subjects’ research because the data source had been previously stripped of identifiers. Presented as a poster in the American Academy of Dermatology Annual Meeting (February 2018), San Diego, CA, and World Congress of Hair Research (April 2019), Barcelona, Spain, and as a poster and oral presentation at the Society for Investigative Dermatology (May 2021) Virtual Meeting. ==== Refs References 1 Villarreal-Villarreal C.D. Boland-Rodriguez E. Rodríguez-León S. Le Voti F. Vano-Galvan S. Sinclair R.D. Dutasteride intralesional microinjections in combination with oral minoxidil vs. oral minoxidil monotherapy in men with androgenetic alopecia: a retrospective analysis of 105 patients J Eur Acad Dermatol Venereol 36 7 2022 e570 e572 10.1111/jdv.18066 35279887 2 Uzel B.P. Takano G.H. Chartuni J.C. Intradermal injections with 0.5% minoxidil for the treatment of female androgenetic alopecia: a randomized, placebo-controlled trial Dermatol Ther 34 1 2021 e14622 10.1111/dth.14622 3 Ghanian S. Wambier C.G. Response to “microneedling with autologous platelet-rich plasma versus microneedling with topical insulin in the treatment of postacne atrophic scars: a simultaneous split-face comparative study” J Am Acad Dermatol 85 6 2021 e395 e396 10.1016/j.jaad.2021.05.070 34403715 4 Arbache S. Godoy C. Microinfusion of drugs into the skin with tattoo equipment Surg Cosmet Dermatol 5 1 2013 70 74 5 Contin L.A. Male androgenetic alopecia treated with microneedling alone or associated with injectable minoxidil by microinfusion of drugs into the skin Surg Cosmet Dermatol 8 2 2016 158 161 10.5935/scd1984-8773.201682782