==== Front Transl Neurodegener Transl Neurodegener Translational Neurodegeneration 2047-9158 BioMed Central London 37381042 365 10.1186/s40035-023-00365-x Research Head-to-head comparison of plasma and PET imaging ATN markers in subjects with cognitive complaints Lu Jiaying 12 Ma Xiaoxi 23 Zhang Huiwei 12 Xiao Zhenxu 23 Li Ming 1 Wu Jie 23 Ju Zizhao 12 Chen Li 4 Zheng Li 23 Ge Jingjie 12 Liang Xiaoniu 23 Bao Weiqi 12 Wu Ping 12 Ding Ding 23 Yen Tzu-Chen 5 Guan Yihui guanyihui@hotmail.com 12 http://orcid.org/0000-0002-8856-7217 Zuo Chuantao zuochuantao@fudan.edu.cn 126 Zhao Qianhua qianhuazhao@fudan.edu.cn 237 on behalf of the Shanghai Memory Study (SMS)Chen Keliang Shi Langfeng Wu Wanqing Zhou Yan Zhang Yan Pei Fang 1 grid.8547.e 0000 0001 0125 2443 Department of Nuclear Medicine and PET Center, Huashan Hospital, Fudan University, Shanghai, China 2 grid.8547.e 0000 0001 0125 2443 National Clinical Research Center for Aging and Medicine and National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai, China 3 grid.8547.e 0000 0001 0125 2443 Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China 4 grid.8547.e 0000 0001 0125 2443 Department of Ultrasound, Huashan Hospital, Fudan University, Shanghai, China 5 APRINOIA Therapeutics Co., Ltd, Suzhou, China 6 grid.8547.e 0000 0001 0125 2443 Human Phenome Institute, Fudan University, Shanghai, China 7 grid.8547.e 0000 0001 0125 2443 MOE Frontiers Center for Brain Science, Fudan University, Shanghai, China 29 6 2023 29 6 2023 2023 12 3419 2 2023 2 6 2023 © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/ Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Background Gaining more information about the reciprocal associations between different biomarkers within the ATN (Amyloid/Tau/Neurodegeneration) framework across the Alzheimer’s disease (AD) spectrum is clinically relevant. We aimed to conduct a comprehensive head-to-head comparison of plasma and positron emission tomography (PET) ATN biomarkers in subjects with cognitive complaints. Methods A hospital-based cohort of subjects with cognitive complaints with a concurrent blood draw and ATN PET imaging (18F-florbetapir for A, 18F-Florzolotau for T, and 18F-fluorodeoxyglucose [18F-FDG] for N) was enrolled (n = 137). The β-amyloid (Aβ) status (positive versus negative) and the severity of cognitive impairment served as the main outcome measures for assessing biomarker performances. Results Plasma phosphorylated tau 181 (p-tau181) level was found to be associated with PET imaging of ATN biomarkers in the entire cohort. Plasma p-tau181 level and PET standardized uptake value ratios of AT biomarkers showed a similarly excellent diagnostic performance for distinguishing between Aβ+ and Aβ− subjects. An increased tau burden and glucose hypometabolism were significantly associated with the severity of cognitive impairment in Aβ+ subjects. Additionally, glucose hypometabolism – along with elevated plasma neurofilament light chain level – was related to more severe cognitive impairment in Aβ− subjects. Conclusion Plasma p-tau181, as well as 18F-florbetapir and 18F-Florzolotau PET imaging can be considered as interchangeable biomarkers in the assessment of Aβ status in symptomatic stages of AD. 18F-Florzolotau and 18F-FDG PET imaging could serve as biomarkers for the severity of cognitive impairment. Our findings have implications for establishing a roadmap to identifying the most suitable ATN biomarkers for clinical use. Supplementary Information The online version contains supplementary material available at 10.1186/s40035-023-00365-x. Keywords ATN biomarkers PET imaging Plasma Clinical severity http://dx.doi.org/10.13039/501100001809 National Natural Science Foundation of China 81971641 82071200 82272039 82021002 Zuo Chuantao Zhao Qianhua STI2030-Major Project2022ZD0211600 Zuo Chuantao http://dx.doi.org/10.13039/501100008750 Shanghai Hospital Development Center SHDC2020CR1038B SHDC2020CR4007 Zuo Chuantao Zhao Qianhua National Key R&D Program of China2022YFC2009902 2022YFC2009900 Zuo Chuantao Medical Innovation Research Project of Shanghai Science and Technology Commission21Y11903300 Ge Jingjie issue-copyright-statement© Ruijin Hospital, Shanghai Jiao Tong University 2023 ==== Body pmcIntroduction With the increase of the aging population worldwide, cognitive impairment is posing a tremendous burden on our society. In addition to dementia [1], subjective cognitive decline (SCD) [2] and mild cognitive impairment (MCI) [3] are two stages of cognitive decline that frequently occur in advanced age. Alzheimer’s disease (AD), the most common form of dementia, is a multifaceted disease with different pathological and mechanistic substrates. The biological definition of AD, that is, the ATN (Amyloid/Tau/Neurodegeneration) framework, aiming for more precise and early disease identification, has gained substantial attraction in research settings [4]. The ATN biomarkers come in three major forms: cerebrospinal fluid (CSF), plasma, and imaging biomarkers. The development of highly specific immunoassays for CSF and plasma biomarkers and recent advances in the field of positron emission tomography (PET) imaging have largely improved the diagnostic accuracy. Notably, there is accumulating evidence supporting complimentary roles for different sets of biomarkers. For example, a rise of CSF or plasma tau species appears to precede abnormal tau PET imaging during the course of AD [5]. This calls for a better understanding of the reciprocal interrelationships between different biomarker matrices within the ATN framework. There is also an unmet need to standardize and validate a strategic roadmap for routine application of ATN biomarkers in memory clinics [6]. Meanwhile, the assessment of relationship between ATN biomarkers and cognitive symptoms (C) is important given that a clinical-biological rather than a purely biological diagnosis of AD is recommended in clinical settings [7]. In recent years, much has been learned on the diagnostic performances of traditional CSF and imaging A (β-amyloid [Aβ] PET, CSF Aβ42, and CSF Aβ42/Aβ40 ratio), T (tau PET and CSF phosphorylated tau [p-tau]), and N (anatomic magnetic resonance imaging [MRI], 18F-fluorodeoxyglucose [18F-FDG] PET, and CSF total tau [t-tau]) biomarkers [4, 7]. Although plasma as a potential source of ATN markers has been increasingly explored to reduce the use of invasive lumbar punctures [8], much validation work remains to be done. Regarding the association between fluid and imaging biomarkers, CSF (Aβ42 for A, p-tau for T, neurofilament light chain [NfL] for N) and imaging (18F-flutemetamol PET for A, 18F-flortaucipir PET for T, anatomic MRI for N) biomarkers are reported to be not interchangeable and the optimal approach varies by clinical stage [9]. Recently, plasma p-tau biomarkers (p-tau181, p-tau217, p-tau231) have been suggested to be valid indicators of amyloid and tau PET in clinical and community populations [10–16], although multiple comorbidities may affect the interpretation of these biomarkers [16]. Meanwhile, similar to the non-commutable correlations between CSF and imaging biomarkers, plasma p-tau (p-tau181, p-tau217, p-tau231) and tau PET (18F-flortaucipir, 18F-RO948, 18F-MK6240) biomarkers are thought to reflect different stages of tau pathology progression [17–19]. In this study, we present a comprehensive head-to-head comparison of plasma (Aβ42/Aβ40 for A, p-tau181 for T, as well as NfL and t-tau for N) and PET imaging (18F-florbetapir for A, 18F-Florzolotau for T, and 18F-FDG for N) ATN biomarkers in a hospital-based cohort of patients with cognitive complaints admitted to a memory clinic. The Aβ status (positive versus negative) and the severity of cognitive impairment served as the main outcome measures for assessing biomarker performances. The four plasma biomarkers included in the current study are relatively well-established and more readily available than other newly developed ones. The FDA-approved amyloid radiotracer 18F-florbetapir plays a cornerstone role in the diagnosis of AD [20]. The second-generation tau ligand 18F-Florzolotau (also known as 18F-APN-1607 or 18F-PM-PBB3) could overcome the limitations of first-generation tau PET tracers and reduce off-target binding [21]. 18F-FDG is the most used PET tracer in nuclear medicine and its accessibility is significantly higher than that of any A and T PET imaging. The current study therefore provides a deeper insight into the comparability of plasma and PET imaging ATN biomarkers, which may be helpful for clinical and research applications. The outstanding strengths of the current study were that all participants were consecutively recruited from a real-life memory clinic, and the aforementioned biomarkers were available to all participants. Methods Participants All procedures and visits occurred at the Memory Clinic of the Department of Neurology, Huashan Hospital, Fudan University (Shanghai, China) and the study was conducted as part of the hospital-based Shanghai Memory Study (SMS) [22]. Patients consecutively enrolled in the SMS were considered eligible if they presented with cognitive complaints and agreed on venous blood sampling (n = 260). After exclusion of subjects who refused multiple PET examinations due to radiation concerns (n = 114), the remaining 146 patients underwent assessment of PET ATN biomarkers using three different tracers (18F-florbetapir for A, 18F-Florzolotau for T, and 18F-FDG for N). After the additional exclusion of patients who had contraindications to structural MRI (n = 9), the final population of interest consisted of 137 subjects. The study participants were finally categorized as being either Aβ-positive (Aβ+) or Aβ-negative (Aβ−) as described below. A flowchart of patient recruitment is shown in Additional file 1: Figure S1. Variables collected for all participants included age, sex, years of education, and the presence of at least one apolipoprotein E (APOE) ε4 allele. Diagnostic criteria All clinical diagnoses were reached by consensus, following clinical interview and review of neuropsychological and biomarker data. Dementia was diagnosed according to the DSM-IV criteria [23], whereas the diagnosis of MCI was made according to the Petersen’s criteria [3]. When a subject did not meet the criteria for MCI or any dementia but reported subjective experience of cognitive decline on one or more cognitive domains, an SCD label was assigned [24]. The clinical diagnosis of AD was based on the NINCDS-ADRDA criteria [25] along with Aβ PET findings [4]. According to the ATN framework [4], all participants with positive findings in 18F-florbetapir amyloid PET (Aβ+) were within the AD spectrum (cognitive impairment due to AD) while those with negative findings (Aβ−) were ruled out from the AD continuum (cognitive impairment not due to AD). Evaluation of cognitive impairment severity Clinical Dementia Rating (CDR) is a semi-structured inventory covering six cognitive, behavioral, and functional aspects. The neurologists need to score each of the above aspects with reference to information collected from participants and proxy. The global CDR score was calculated and the severity of cognitive impairment was assessed using a 5-point scale (0, 0.5, 1.0, 2.0, and 3.0) based on the Washington University CDR-assignment algorithm, with higher levels indicating higher severity [26, 27]. Neuropsychological testing The study participants underwent extensive neuropsychological testing to assess global cognition, instrumental activities of daily living, as well as memory, visuospatial, language, attention, and executive functions. Global cognition was assessed using the Mini-Mental State Examination (MMSE) and the Montreal Cognitive Assessment (MOCA). Instrumental activities of daily living were investigated using the Functional Assessment Questionnaire (FAQ) questionnaire. Raw scores of the Auditory Verbal Learning Test, Rey-Osterrieth Complex Fig test, Boston Naming Test, Trail Making Test, Clock Drawing Test, Verbal Fluency Test, Symbol Digit Modalities Test, and Similarity Test were collected and Z-transformed based on previously reported normative data [28]. Z-scores for each test were grouped according to specific cognitive domains (i.e., memory, visuospatial function, language, attention, and executive functions) and averaged for subsequent analyses [28]. Quantification of plasma ATN biomarkers Whole blood collected into spray-coated K2EDTA tubes was centrifuged at 1000 rpm for 15 min at 4 °C. The plasma fraction was transferred to a new 1.5-ml tube (Eppendorf, Hamburg, Germany) and stored at − 80 °C until use. Plasma levels of Aβ40 (A), Aβ42 (A), p-tau181 (T), t-tau (N), and NfL (N) were measured simultaneously on a Simoa® HDx analyzer (Quanterix, Billerica, MA), according to the manufacturer’s instructions. The Aβ42/Aβ40 ratio was subsequently determined and used as the plasma Aβ biomarker (A). Laboratory personnel were blinded to clinical information and imaging data. A detailed protocol has been reported elsewhere [29]. Image acquisition The mean (standard deviation) interval from PET imaging to blood collection was 2.6 (5.0) weeks. PET imaging ATN biomarkers were assessed using the following tracers: 18F-florbetapir for A (18F-AV-45; 50 − 70 min post-injection), 18F-Florzolotau for T (90 − 110 min post-injection), and 18F-FDG for N (60 − 70 min post-injection). Static images were acquired on different days on a Biograph mCT Flow PET/CT system (Siemens, Erlangen, Germany). High-resolution structural MRI images obtained with a 3.0-T horizontal magnet scanner (Discovery MR750; GE Medical Systems, Milwaukee, WI) were used for spatial normalization. The protocols used for acquisition have been described previously in detail [30–32]. Assessment and classification of Aβ PET images Raw Aβ PET images were visually interpreted using dedicated software (Siemens syngo.via) by two independent neuroradiologists (CZ, more than 20 years of experience; JL, more than 5 years of experience) blinded to clinical and laboratory data. Each participant was classified as either Aβ-positive (Aβ+) or Aβ-negative (Aβ−) according to the criteria proposed previously [33]. A third expert (HZ, more than 10 years of experience) was invited to review images in case of discrepancies; the final classification was based on majority voting. Image processing and semi-quantitative analysis Images were processed via Statistical Parametric Mapping 12 (SPM12; http://www.fil.ion.ucl.ac.uk/spm/software/spm12/) implemented in MATLAB (version 2018b, MathWorks, Natick, MA). Prior to smoothing (full-width at half-maximum: 8 mm), raw PET images were first co-registered to the concurrent structural MRI images and then spatially normalized to the Montreal Neurological Institute standard space using the transformation matrices of segmented individual structural MRI images. The following reference regions were selected: whole cerebellum for 18F-florbetapir PET [34], cerebellar grey matter for 18F-Florzolotau PET [31], and pons for 18F-FDG PET [35]. Standardized uptake value ratio (SUVR) images were obtained after reference region-based intensity normalization. Aβ SUVR values were quantified in the following regions of interest (ROIs) defined according to the Automated Anatomical Labelling Atlas three (AAL3) [36]: bilateral frontal lobes, anterior cingulate gyrus, posterior cingulate gyrus, lateral parietal gyrus and lateral temporal gyrus. In accordance with previous methodology [34], the unweighted average SUVR value of the ROIs located above was considered as the global Aβ SUVR. Tau SUVR values were determined in the following ROIs according to the Desikan-Killiany Atlas [37]: bilateral entorhinal cortex, amygdala, middle temporal gyrus, and inferior temporal gyrus. In keeping with a previous study [38], the unweighted average SUVR value of the first two ROIs and the weighted average SUVR value of the last two ROIs were considered as the medial temporal lobe (MTL) and temporal neocortex (NEO-T) SUVR values for tau, respectively. 18F-FDG SUVR values were quantified in the following ROIs defined according to the AAL3 [36]: bilateral angular gyri, posterior cingulate gyrus, and inferior temporal gyrus. The unweighted average SUVR value of all ROIs was considered as the meta-analytically derived region of interest (metaROI) SUVR for determining an abnormal glucose metabolic activity [39]. Statistics The Kolmogorov–Smirnov test was used to test the normal distribution of continuous variables. Intergroup comparisons between Aβ+ and Aβ− subjects were performed using independent Student’s t-tests (normally distributed continuous variables), Mann–Whitney U tests (skewed continuous variables), and chi-square tests (categorical variables), as appropriate. The reciprocal associations between plasma and PET imaging ATN biomarkers were investigated at both voxel and region levels. For voxel-wise analysis, the multiple regression model implemented in SPM12 was applied to the entire cohort, as well as separately to Aβ+ and Aβ− subjects. Age, sex, and the interval from PET imaging to blood collection (expressed in weeks) were entered as covariates, with the statistical threshold being set at a family wise error (FWE)-corrected P value (PFWE) < 0.05. For region-level assessments, partial correlation analysis after adjustment for age, sex, and the interval from PET imaging to blood collection was applied to the entire cohort, as well as separately to Aβ+ and Aβ− subjects. Generalized linear models (GLMs) adjusted for age and sex were used to compare the performances of plasma and PET imaging ATN biomarkers in Aβ+ and Aβ− subjects. The ability of biomarkers in distinguishing the Aβ status was determined by receiver operating characteristic (ROC) curve analysis. Agreement and Cohen’s kappa were calculated to assess the concordance between biomarkers. The optimal cutoff values for biomarker-based classification were determined with the greatest Youden’s index based on the ROC curve analysis. The associations between plasma and PET imaging ATN biomarkers and the severity of cognitive impairment (CDR scores) were investigated using GLMs adjusted for age and sex in the entire cohort, as well as separately to Aβ+ and Aβ− subjects. Finally, age- and sex-adjusted partial correction analysis was implemented to investigate the correlations between biomarkers and the results of neuropsychological testing. Data were analyzed using SPSS, version 23 (IBM, Armonk, NY), unless otherwise indicated. Bonferroni-corrected P values (Pc) were used to adjust for multiple comparisons. Two-tailed P values < 0.05 were considered statistically significant, unless otherwise indicated. Further adjustments for education and APOE ε4 were also made for all analysis where applicable and relevant results are presented in Additional file 1: Fig. S2, Fig. S4–S6, Table S1–S4. Results Participants The final study cohort consisted of 137 patients with cognitive complaints who were classified as either Aβ-positive (Aβ+; n = 90) or Aβ-negative (Aβ−; n = 47) based on visual interpretation of 18F-florbetapir PET imaging findings. The Aβ+ group comprised 29 patients with MCI due to AD and 61 with AD dementia, whereas the Aβ− group included 10 subjects with SCD, 26 patients with MCI not due to AD and 11 with dementia not due to AD. There were no intergroup differences in terms of age, years of education, and interval from PET imaging to blood collection (Table 1); however, the Aβ+ group showed more severe cognitive impairment and included a higher proportion of women and APOE ε4 allele-carries.Table 1 General characteristics of the study participants stratified according to the Aβ status Aβ+ subjects Aβ− subjects P value Number of subjects 90 47 – Age, years 65.5 (9.6) 66.1 (7.9) 0.696 a Sex, female, % 61.1 42.6 0.047 b Education, years 10.6 (3.9) 11.7 (4.6) 0.165 a APOE ε4 carries, % e 61.8 21.3  < 0.001 b Interval from PET imaging to blood collection, weeks 2.3 (4.6) 3.2 (5.6) 0.318 a Neuropsychological tests CDR, number of subjects CDR = 0, 0 CDR = 0.5, 29 CDR = 1, 45 CDR = 2, 13 CDR = 3, 3 CDR = 0, 8 CDR = 0.5, 30 CDR = 1, 6 CDR = 2, 3 CDR = 3, 0  < 0.001 c* MMSE 22.0 [19.0, 25.0] 26.0 [24.0, 28.0]  < 0.001 c* MOCA e 16.0 [9.5, 19.0] 19.0 [16.0, 24.0]  < 0.001 c* FAQ 12.5 [8.8, 18.0] 7.0 [4.0, 9.0]  < 0.001 c* Memory e, f − 2.1 [− 2.5, − 1.7] − 1.6 [− 2.1, − 0.6]  < 0.001 c* Visuospatial function e, f − 1.7 [− 6.9, 0.1] 0.0 [− 2.4, 0.7] 0.002 c* Language e, f − 1.6 [− 2.8, − 0.6] − 0.9 [− 1.7, − 0.2] 0.004 c* Attention e, f − 2.1 [− 6.3, − 0.6] − 0.7 [− 1.4, 0.0]  < 0.001 c* Executive function e, f − 3.8 [− 10.1, − 1.3] 0.1 [− 2.5, 2.5]  < 0.001 c* PET ATN biomarkers A: Global SUVR 1.4 [1.3, 1.5] 1.2 [1.1, 1.2]  < 0.001 d* T: MTL SUVR 1.6 [1.4, 1.8] 1.1 [1.0, 1.2]  < 0.001 d* T: NEO-T SUVR 1.5 [1.3, 1.8] 1.0 [1.0, 1.1]  < 0.001 d* N: metaROI SUVR 1.2 [1.1, 1.4] 1.4 [1.4, 1.6]  < 0.001 d* Plasma ATN biomarkers A: Aβ42/Aβ40 ratio 0.04 [0.04, 0.06] 0.05 [0.04, 0.06] 0.054 d T: P-tau181, pg/ml 4.8 [3.9, 6.0] 2.5 [1.8, 2.9]  < 0.001 d* N: T-tau, pg/ml 3.4 [2.3, 5.4] 3.2 [2.1, 4.6] 0.238 d N: NfL, pg/ml 18.0 [14.5, 21.6] 13.6 [8.8, 20.7] 0.064 d Data are presented as mean (standard deviation) or median [quartile 1, quartile 3], unless otherwise indicated Each participant was classified as either Aβ-positive (Aβ+) or Aβ-negative (Aβ−) based on 18F-florbetapir PET imaging findings. Unadjusted P values are presented, and those surviving multiple comparisons (Bonferroni’s correction, Pc < 0.05) are marked with an asterisk (*) aIndependent Student’s t-test. bChi-square test. cMann–Whitney U test. dGeneralized linear model adjusted for age and sex. eOne patient had missing data. fZ-score transformed APOE Apolipoprotein E, PET Positron emission tomography, CDR Clinical Dementia Rating, MMSE Mini-Mental State Examination, MOCA Montreal Cognitive Assessment, FAQ Functional Activities Questionnaire, A/T/N Amyloid/Tau/Neurodegeneration, SUVR Standardized uptake value ratio, MTL Medial temporal lobe, NEO-T Temporal neocortex, metaROI Meta-analytically derived region of interest, t-tau Total tau, NfL Neurofilament light chain Reciprocal associations between plasma and PET imaging ATN biomarkers The reciprocal associations between plasma and PET imaging ATN biomarkers analyzed on a voxel-wise level (PFWE < 0.05) are shown in Fig. 1. In the entire cohort, the plasma p-tau181 level showed positive correlations with (1) Aβ PET SUVR value throughout the entire cortex – with only exception in the MTL as well as the precentral and postcentral gyrus (Fig. 1a) and (2) tau PET SUVR value throughout the entire cortex, with exception in the precentral and postcentral gyrus (Fig. 1b). Therefore, the findings of Aβ and tau PET imaging in the MTL differed significantly. Besides, the strength of the associations was lower for Aβ PET compared with tau PET imaging. On analyzing the 18F-FDG PET data, the plasma p-tau181 level was negatively correlated with SUVR values measured in the angular gyrus, precuneus, inferior parietal gyrus, as well as middle and posterior cingulate gyrus (Fig. 1c). No other significant associations between plasma and PET imaging ATN biomarkers were observed in the entire study cohort. In addition, all correlation analyses yielded negative results when Aβ+ and Aβ− patients were separately considered (Fig. 1). On a region-level basis (Table 2), the results were largely similar to those observed at the voxel-level. Consistent findings were seen when further adjusted for education and APOE ε4 (Additional file 1: Fig. S2, Table S1).Fig. 1 Reciprocal associations between plasma and PET imaging ATN biomarkers: voxel-wise analysis. Voxel-wise regression analysis of standardized uptake value ratios from 18F-florbetapir PET for A (a), 18F-Florzolotau PET for T (b), and 18F-FDG PET for N (c) in relation to plasma ATN biomarkers (Aβ42/Aβ40 ratio, p-tau181, t-tau, NfL) adjusted for age, sex, and the interval from PET imaging to blood collection; calculations were performed in the entire cohort, as well as in Aβ+ and Aβ− subjects. The statistical threshold was set at a family wise error (FWE)-corrected P value < 0.05. The positive correlations are displayed in orange-red color scale. The negative correlations are displayed in cyan-blue color scale. PET Positron emission tomography, t-tau, total tau, NfL Neurofilament light chain, FWE Family-wise error Table 2 Region-level reciprocal associations between plasma and PET imaging ATN biomarkers A Aβ42/Aβ40 ratio T p-tau181, pg/ml N t-tau, pg/ml N NfL, pg/ml r P r P r P r P Entire cohort (n = 137) A: Global SUVR − 0.044 0.617 0.528  < 0.001*** 0.039 0.653 0.036 0.679 T: MTL SUVR − 0.018 0.836 0.613  < 0.001*** 0.078 0.373 0.172 0.046 T: NEO-T SUVR − 0.035 0.686 0.623  < 0.001*** 0.081 0.350 0.204 0.018 N: metaROI SUVR 0.026 0.767 − 0.491  < 0.001*** − 0.013 0.885 − 0.189 0.029 Aβ+ (n = 90) A: Global SUVR 0.070 0.520 0.143 0.186 − 0.046 0.669 − 0.044 0.683 T: MTL SUVR 0.122 0.259 0.254 0.018 − 0.047 0.665 0.063 0.563 T: NEO-T SUVR 0.123 0.257 0.274 0.010 − 0.043 0.693 0.142 0.188 N: metaROI SUVR − 0.146 0.178 − 0.217 0.043 0.171 0.112 − 0.042 0.700 Aβ− (n = 47) A: Global SUVR 0.187 0.223 0.242 0.113 − 0.094 0.545 − 0.260 0.089 T: MTL SUVR 0.324 0.032 0.362 0.016 − 0.108 0.484 0.090 0.561 T: NEO-T SUVR 0.220 0.151 0.380 0.011 − 0.102 0.509 0.062 0.691 N: metaROI SUVR − 0.013 0.931 -0.033 0.831 0.032 0.838 − 0.239 0.118 Partial correction analysis adjusted for age, sex, and the interval from PET imaging to blood collection was undertaken to assess the reciprocal associations between plasma and PET imaging ATN biomarkers. The reported P values are unadjusted. Significant P values (P < 0.05) are marked in bold, those surviving multiple comparisons (Bonferroni’s correction) are marked with asterisks (***, Pc < 0.001) PET Positron emission tomography, A/T/N Amyloid/Tau/Neurodegeneration, SUVR Standardized uptake value ratio, MTL Medial temporal lobe, NEO-T Temporal neocortex, metaROI Meta-analytically derived region of interest, t-tau Total tau, NfL Neurofilament light chain Plasma and PET imaging ATN biomarkers in relation to the Aβ status Compared with Aβ− participants, the Aβ+ participants consistently showed an increased pathological burden as reflected by higher PET SUVR values for AT biomarkers and lower PET SUVR value for N biomarker (Pc < 0.001; Table 1). Similar findings were observed for plasma biomarkers, with significantly higher p-tau181 level (Pc < 0.001) and a trend towards lower Aβ42/Aβ40 ratio (P = 0.054) and higher NfL level (P = 0.064) in the Aβ+ participants. However, plasma t-tau concentrations showed no intergroup difference. When further adjusted for education and APOE ε4 status, the differences in all PET biomarkers and plasma p-tau remained between Aβ+ and Aβ− subjects (Pc < 0.001) and there was still no difference in plasma t-tau (P = 0.308); however, the difference in plasma NfL reached the level of statistical significance (Pc < 0.039). On analyzing the areas under the ROC curve (AUC) for distinguishing between Aβ+ and Aβ− subjects (Table 3), we found excellent diagnostic performances of the following biomarkers: global SUVR value for A (AUC = 0.93), MTL SUVR value for T (AUC = 0.94), NEO-T SUVR value for T (AUC = 0.95), and plasma p-tau181 level for T (AUC = 0.93). The accuracy of the metaROI SUVR value for N was less remarkable (AUC = 0.83), whereas plasma biomarkers for A and N lacked discriminatory ability (AUC ≤ 0.65). We next examined the agreement between plasma and PET imaging ATN biomarkers that were found to distinguish between Aβ+ and Aβ− patients. As expected (Fig. 2), plasma p-tau181 level for T and PET SUVR value for T showed a fairly high agreement (plasma p-tau181 level versus MTL SUVR value for T: Cohen’s kappa = 0.65; plasma p-tau181 level versus NEO-T SUVR value for T: Cohen’s kappa = 0.72). In addition, plasma p-tau181 level for T showed moderate agreement with both PET SUVR value for A (Cohen’s kappa = 0.59) and PET SUVR value for N (Cohen’s kappa = 0.45). Additional file 1: Fig. S3 shows a pairwise analysis of the observed agreement between different PET imaging ATN biomarkers – which ranged from substantial (A and T: Cohen’s kappa = 0.61 [global SUVR value for A versus MTL SUVR value for T], 0.70 [global SUVR value for A versus NEO-T SUVR value for T]) to moderate (T and N: Cohen’s kappa = 0.48 [NEO-T SUVR value for T versus metaROI SUVR value for N], 0.50 [MTL SUVR value for T versus metaROI SUVR value for N]) and fair (A and N: Cohen’s kappa = 0.33).Table 3 Performance of plasma and PET imaging ATN biomarkers for predicting the Aβ status AUC (95% CI) P value Sensitivity (%) Specificity (%) Optimal cutoff PET ATN biomarkers A: Global SUVR 0.93 (0.88–0.97)  < 0.001*** 86.7 89.4 1.25 T: MTL SUVR 0.94 (0.82–0.96)  < 0.001*** 82.2 95.7 1.30 T: NEO-T SUVR 0.95 (0.91–0.99)  < 0.001*** 86.7 97.9 1.23 N: metaROI SUVR 0.83 (0.76–0.90)  < 0.001*** 80.9 72.2 1.37 Plasma ATN biomarkers A: Aβ42/Aβ40 ratio 0.63 (0.54–0.73) 0.011 80.9 45.6 0.04 T: P-tau181, pg/ml 0.93 (0.88–0.98)  < 0.001*** 93.3 85.1 3.23 N: T-tau, pg/ml 0.56 (0.46–0.66) 0.265 17.8 97.9 5.76 N: NfL, pg/ml 0.65 (0.54–0.75) 0.005* 81.1 51.1 13.63 Each participant was classified as either Aβ-positive (Aβ+) or Aβ-negative (Aβ−) based on 18F-florbetapir PET imaging findings. Each plasma and PET imaging ATN biomarker was examined by receiver operating characteristic (ROC) curve analysis in relation to its ability to predict the Aβ status. The optimal cutoff for each biomarker was selected as the point that maximized the Youden’s index according to the ROC curve analysis; the corresponding sensitivity and specificity were subsequently calculated. Unadjusted P values are presented. Significant P values (P < 0.05) are marked in bold, and those surviving multiple comparisons (Bonferroni’s correction) are marked with asterisks (***, Pc < 0.001; *, Pc < 0.05) PET Positron emission tomography, A/T/N Amyloid/Tau/Neurodegeneration, SUVR Standardized uptake value ratio, MTL Medial temporal lobe, NEO-T Temporal neocortex, metaROI meta-analytically derived region of interest, p-tau181 Tau phosphorylated at threonine 181, t-tau Total tau, NfL Neurofilament light chain, AUC Area under the curve, CI Confidence interval Fig. 2 Agreement between plasma p-tau181 levels and PET imaging ATN biomarkers for predicting the Aβ status. The concordance rates are based on the established thresholds for the biomarkers. The sums of negative and positive concordance rates are presented. Color bars summarize the concordance rates between plasma p-tau181 level and different PET imaging ATN biomarkers in the entire cohort (upper row) as well as Aβ+ subjects (intermediate row) and Aβ− subjects (lower row). Negative (− −) and positive (+ +) agreement are denoted in green and orange, respectively. Disagreement is reported in magenta (+ −; positive SUVR value on PET and negative p-tau181 level) or in blue (− + ; negative SUVR value on PET and positive p-tau181 level). A/T/N, Amyloid/Tau/Neurodegeneration, SUVR Standardized uptake value ratio, MTL Medial temporal lobe, NEO-T Temporal neocortex; metaROI, meta-analytically derived region of interest; p-tau181, tau phosphorylated at threonine 181 Plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment We next analyzed plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment; to this aim, the study participants were categorized in different CDR categories. The Aβ+ subjects were divided into three categories (CDR = 0.5, n = 29; CDR = 1, n = 45; CDR ≥ 2, n = 16). Group-average SUVR maps for each CDR category are presented in Fig. 3a. MTL SUVR value for T, NEO-T SUVR value for T and metaROI SUVR value for N showed that the tau burden increased and the glucose metabolism decreased in a stepwise fashion with increased CDR. Neither global SUVR value for A (Fig. 3a) nor plasma ATN biomarkers (Fig. 3b) showed such associations. On analyzing Aβ− subjects, we found that the CDR categories did not show significant associations with either plasma or PET imaging ATN biomarkers (Additional file 1: Fig. S4), with the only exceptions being metaROI SUVR value and plasma NfL level (both for N). Specifically, glucose hypometabolism showed a trend towards a higher frequency in patients with more severe cognitive impairment, although the difference did not persist after correcting for multiple comparisons. Plasma NfL level was significantly higher in patients with a CDR ≥ 1 than in those with a CDR = 0.5 (Pc < 0.01). The results from the entire cohort are shown in Additional file 1: Fig. S5. Similar results were obtained after corrections for education and APOE ε4 status (Additional file 1: Tables S2-S4).Fig. 3 Plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment in Aβ+ subjects. Average SUVR maps for PET imaging A (left), T (middle), and N (right) biomarkers in relation to different CDR categories (a; upper row). Generalized linear models after adjustment for age and sex were applied to analyze the values of PET (a; lower row) and plasma (b) ATN biomarkers in relation to the severity of cognitive impairment. Unadjusted P values are presented for differences between the three CDR categories, whereas those that remained significant after correcting for multiple comparisons (Bonferroni’s correction) are marked with asterisks (***, Pc < 0.001; **, Pc < 0.01). The thick solid line, the thin solid lines, and the dots denote the median, the 25th and 75th percentiles, and individual values, respectively. CDR Clinical Dementia Rating, PET Positron emission tomography, A/T/N Amyloid/Tau/Neurodegeneration, SUVR Standardized uptake value ratio, MTL Medial temporal lobe, NEO-T Temporal neocortex, metaROI Meta-analytically derived region of interest, p-tau181 tau phosphorylated at threonine 181, t-tau total tau, NfL Neurofilament light chain Plasma and PET imaging ATN biomarkers in relation to neuropsychological tests On analyzing the entire study cohort, we found consistent associations between the results of neuropsychological tests and MTL SUVR value for T, NEO-T SUVR value for T, as well as metaROI SUVR value for N (Fig. 4a) – with statistical significance remaining after correcting for multiple comparisons. Similar significant associations were observed between plasma p-tau181 level for T and the results of the FAQ, as well as memory, visuospatial function, attention, and executive functioning. The plasma NfL level for N was significantly associated with the results of MOCA, as well as memory, language, and executive functioning. The results in the Aβ+ group (Fig. 4b) were generally consistent with those obtained in the entire cohort. However, no significant associations were observed when ATN biomarkers were analyzed in relation to neuropsychological tests in Aβ− subjects (Fig. 4c). Further corrections for education and APOE ε4 did not change the findings (Additional file 1: Fig. S6). The only exception was that in Aβ− subjects, the nonsignificant associations between plasma NfL level and scores of neuropsychological tests reached the significance after further adjustment for education and APOE ε4.Fig. 4 Associations of plasma and PET imaging ATN biomarkers with neuropsychological tests. Partial correction analysis after adjustment for age and sex was applied to evaluate the associations between plasma and PET imaging ATN biomarkers and the results of neuropsychological tests in the entire cohort (a) as well as in Aβ+ (b) and Aβ− (c) subjects. Unadjusted P values are presented with asterisks (***, P < 0.001; **, P < 0.01; *, P < 0.05) whereas those that remained significant after correcting for multiple comparisons (Bonferroni’s correction, Pc < 0.05) are marked with solid frames. The results of neuropsychological testing on different cognitive domains were transformed to Z-scores. The color bars denote partial correlation coefficients (r). MMSE Mini-Mental State Examination, MOCA Montreal Cognitive Assessment, FAQ Functional Activities Questionnaire, PET Positron emission tomography, SUVR Standardized uptake value ratio, MTL Medial temporal lobe; NEO-T Temporal neocortex, metaROI Meta-analytically derived region of interest, t-tau total tau, NfL Neurofilament light chain Discussion The present study has three main findings. First, plasma p-tau181 level was found to be significantly associated with PET imaging ATN biomarkers in the entire study cohort, although this association did not persist when Aβ+ and Aβ− subjects were analyzed separately. Second, we identified four biomarkers (global SUVR value for A, MTL SUVR value for T, NEO-T SUVR value for T, and plasma p-tau181 level for T) with similar and good performance in distinguishing between Aβ+ and Aβ− subjects. Third, we found that an increasing tau burden (as reflected by higher MTL and NEO-T SUVR values on 18F-Florzolotau PET) and a decreasing glucose metabolism (as reflected by lower metaROI SUVR value on 18F-FDG PET) were significantly associated with the severity of cognitive impairment in Aβ+ subjects. Glucose hypometabolism, along with elevated plasma NfL level, was also related to more severe cognitive impairment in Aβ− subjects. Taken together, while both 18F-Florzolotau tau PET and plasma p-tau181 are interchangeable markers for 18F-florbetapir amyloid PET on detecting the presence of amyloid pathology in unselected patients with cognitive complaints, plasma p-tau181 is preferred in screening considering the cost-effectiveness. Further, our study provides scoping information about the potential usefulness of 18F-Florzolotau PET and 18F-FDG PET as markers of clinical severity, and none of the plasma biomarkers included in the current study could be used interchangeably in this regard. Notably, since all participants had cognitive complaints and were recruited from a real-life memory clinic, the current findings may only apply to the symptomatic population. In addition to the established imaging and CSF markers (Aβ PET imaging and CSF Aβ42/Aβ40 ratio) [40], plasma-based biomarkers have frequently been investigated for their ability to identify subjects with amyloid pathology – with most studies focusing on the plasma Aβ42/Aβ40 ratio. However, reliable studies have shown that the Aβ42/Aβ40 ratio in plasma generally underperforms the established CSF and imaging A biomarkers [41] and is prone to significant analytical variation [42]. By relying on the visual interpretation of 18F-florbetapir PET images to achieve a dichotomous classification of the Aβ status, our current findings further support the view that the plasma Aβ42/Aβ40 ratio has a limited value as an A biomarker [43]. Additionally, we found that neither plasma nor PET imaging A biomarkers were significantly associated with the severity of cognitive impairment. This finding is consistent with previous observations showing that cerebral Aβ accumulation reaches a plateau during the prodromal stage [44] and that the plasma amyloid biomarker profile does not correlate with cognitive function across the clinical spectrum of AD [45]. The agreement between PET, CSF, and plasma T biomarkers varies widely from 66% to 95% [46] and can be influenced by differences in assays and laboratory procedures [47]. The association of plasma p-tau181 (T) with CSF p-tau181 (T) as well as 18F-flortaucipir PET (T) and 18F-flutemetamol PET (A) has been previously reported for Aβ+ subjects and for an unselected sample comprising both Aβ+ and Aβ− individuals – but not for Aβ− subjects analyzed separately [11]. These findings are also consistent with an analysis by Thijssen et al. who reported significant associations of 18F-flortaucipir PET (T) with both plasma p-tau181 (T) and p-tau217 (T) [48]. In our study, associations of plasma p-tau181 (T) with 18F-Florzolotau PET (T), 18F-florbetapir PET (A), and 18F-FDG PET (N) were examined. However, they reached the threshold for statistical significance only in the entire study cohort. On analyzing Aβ+ individuals separately, our results were not consistent with previous studies possibly because we included only subjects with MCI and dementia due to AD but not those in the preclinical stage, as other authors did [11, 48]. Our data also showed that the plasma p-tau181 level (T) was more closely associated with 18F-Florzolotau PET (T) than 18F-florbetapir PET (A), although Thijssen et al. found that the plasma p-tau biomarkers were mainly related to PET imaging A biomarkers (18F-AZD4694, 18F-florbetapir) than T biomarkers (18F-MK6240, 18F-flortaucipir) [48]. This discrepancy might be because that the present study used a different tau PET tracer (18F-Florzolotau) and did not include subjects in the preclinical stage. It is worth noting that 18F-Florzolotau has shown favorable affinity to all types of tau aggregates [21] and is able to detect tau deposition in vivo in the brains of patients with different tauopathies (i.e., three- and four-repeat (3R/4R) tau in AD [49–51], 4R-tau in progressive supranuclear palsy [31, 52], 4R and 3R/4R-tau in frontotemporal lobar degeneration with tauopathy caused by microtubule-associated protein tau mutations [53]) while 18F-MK6240 and 18F-flortaucipir have relatively low affinity for non-AD tauopathies [54–56]. Plasma levels of p-tau biomarkers have been reported to be strongly correlated with both CSF and PET imaging T biomarkers [11, 14, 15, 46, 48, 57]. In line with these findings, the present study found similar patterns for plasma p-tau181 concentrations and PET imaging results with a second-generation tau tracer (18F-Florzolotau). Interestingly, these markers also appeared to have an excellent discriminatory ability to distinguish between Aβ+ and Aβ− individuals. Research investigating plasma (p-tau181, p-tau217, and p-tau231) and PET imaging (18F-RO948, 18F-MK-6240, and 18F-flortaucipir) T biomarkers has generally detected an increasing tau burden from the preclinical stage to clinically overt dementia. Aside from evidence that plasma p-tau levels tend to increase in a less pronounced fashion in symptomatic patients [11, 15, 45, 58–61], the correlations of plasma T biomarkers with the results of neuropsychological testing are generally moderate [17, 45, 48]. The pathophysiological cascade of Aβ- and tau-related processes is not constant during disease progression, that is, as opposed to early in the disease, in the advanced stages such as AD dementia when Aβ fibrils and soluble p-tau levels have stabilized, cognitive decline is associated with the accumulation rate of insoluble tau aggregates [62]. Our data add to previous evidence by demonstrating that only SUVR values on 18F-Florzolotau PET imaging – and not plasma T biomarker – increased in a stepwise fashion with the increasing severity of cognitive impairment. This result suggests that plasma and PET imaging T biomarkers may convey information that is at least in part not overlapping, with plasma p-tau181 concentrations being more closely related to Aβ pathology and tau PET imaging findings being mainly a reflection of the cognitive impairment severity [46]. However, this possibility requires confirmation given the non-linear increase in plasma p-tau181 concentrations during the course of AD [63]. Another prospective research with different T biomarkers from those of our study consistently indicated that the soluble tau as reflected by elevated plasma p-tau217 and the insoluble tau aggregates as reflected by elevated tau PET (18F-RO948 and 18F-flortaucipir) signals, are optimal predictors for longitudinal tau accumulation in the brains of patients with AD at preclinical and prodromal phases, respectively [18]. Future work in this area should also validate different plasma T biomarkers, which may have differential roles for identifying amyloid pathology [64]. Moreover, since comorbidities such as chronic kidney disease are reported to have a non-negligible impact on the interpretation of plasma p-tau181 and p-tau217 levels [16], further studies exploring their potential impact on tau PET biomarkers are warranted. Given that neurodegeneration is the final consequence of various pre-existing pathological alterations, research has generally explored the association of N biomarkers with the severity of cognitive impairment and clinical trajectories over time [65]. However, 18F-FDG PET as an imaging N biomarker also shows diagnostic value among patients present to memory clinics with an uncertain diagnosis [66]. In line with prior studies [14, 67], metaROI SUVR value on 18F-FDG PET imaging was the only N biomarker capable of distinguishing between Aβ+ and Aβ− individuals, although it underperformed both A and T biomarkers. There is also evidence that, different from other N biomarkers, glucose hypometabolism on 18F-FDG PET may predict a steeper cognitive decline trajectory; therefore, the traditional classification of 18F-FDG PET imaging as an N biomarker has been put into question [68]. Interestingly, we found that 18F-FDG PET outperformed NfL – a plasma N biomarker – in reflecting the severity of cognitive impairment in Aβ+ individuals. However, the plasma NfL level was superior to 18F-FDG PET as a marker of disease severity in Aβ− individuals – a finding which calls for additional investigations. While we are not aware of any other study that has provided a head-to-head comparison of plasma and PET imaging ATN biomarkers in relation to the presence of amyloid pathology and the severity of cognitive impairment across the AD spectrum, several design limitations should be acknowledged. Since this single-center investigation was cross-sectional, it is not possible to establish the causal nature or the directionality of the observed associations. We did not obtain longitudinal measures of cognitive impairment, which restricts the prognostic impact of our findings. Meanwhile, the sample size of the final study cohort was limited, and attention needs to be paid to potential sources of bias. We have only enrolled patients presented to a memory clinic and consequently we were unable to include subjects in the preclinical stage. Our findings may be most applicable and generalizable to those with MCI or dementia due to AD. Another limitation is the uneven distribution of amyloid pathology, resulting in more participants within the Aβ+ group. The uneven distributions of different severities of clinical cognitive impairment, a common drawback of the serial-enrollment design when the study sample size is limited, also requires attention. The present analysis did not include measurements of recently developed plasma T biomarkers (i.e., p-tau217, p-tau231), as well as of neuroinflammatory markers. Because the availability of 18F-Florzolotau PET imaging is still limited, our results are not conducive to establishing a definitive equivalence between this imaging modality and the combination of plasma p-tau181 and 18F-FDG PET. Besides, two atlases were used for PET imaging analysis, which may have attenuated some of the results although preceding data rendered such effects likely to be minimal [69]. Last but not least, we took the visual assessment of 18F-florbetapir PET imaging as a ground truth for Aβ status. As semi-quantitative binary cutoffs (i.e., a global SUVR greater than 1.1 indicates positive Aβ accumulation) [34, 70] have been recommended for 18F-florbetapir, it is necessary to further replicate our findings using semi-quantitative measurements as the ground truth. Conclusion The results from the present study raise the possibility that 18F-florbetapir PET imaging (A), 18F-Florzolotau PET imaging (T), and plasma p-tau181 (T) can be considered as interchangeable biomarkers in the assessment of Aβ status in both MCI and dementia due to AD. The findings on 18F-Florzolotau PET (T) and 18F-FDG PET (N) could also serve as imaging markers for the severity of cognitive impairment. Supplementary Information Additional file 1 Fig. S1. Study flowchart. Fig. S2. Reciprocal associations between plasma and PET imaging ATN biomarkers: voxel-wise analysis. Fig. S3. Agreement between different PET imaging ATN biomarkers for predicting the Aβ status. Fig. S4. Plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment in Aβ− subjects. Fig. S5. Plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment in the entire cohort. Fig. S6. Associations of plasma and PET imaging ATN biomarkers with neuropsychological tests. Table S1. Region-level reciprocal associations between plasma and PET imaging ATN biomarkers. Table S2. Plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment in Aβ+ subjects. Table S3. Plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment in Aβ− subjects. Table S4. Plasma and PET imaging ATN biomarkers in relation to the severity of cognitive impairment in the entire cohort. Abbreviations AAL3 Automated Anatomical Labelling Atlas three Aβ β-Amyloid AD Alzheimer’s disease APOE Apolipoprotein E CDR Clinical dementia rating CSF Cerebrospinal fluid FAQ Functional Assessment Questionnaire FDG Fluorodeoxyglucose FWE Family wise error GLM Generalized linear model MCI Mild cognitive impairment metaROI Meta-analytically derived region of interest MMSE Mini-Mental State Examination MOCA Montreal Cognitive Assessment MRI Magnetic resonance imaging MTL Medial temporal lobe NEO-T Temporal neocortex NfL Neurofilament light chain PET Positron emission tomography p-tau Phosphorylated tau SCD Subjective cognitive decline SPM12 Statistical Parametric Mapping 12 SUVR Standardized uptake value ratio Acknowledgements We would like to thank all staff members of the Shanghai Memory Study (SMS) (full list presented in the Consortium list below). We would like to express our sincere appreciation to the study participants and their relatives. We are grateful to APRINOIA Therapeutics for providing the 18F-Florzolotau precursor. Keliang Chen1,2, Langfeng Shi1,2, Wanqing Wu1,2, Yan Zhou1,2, Yan Zhang1,2, Fang Pei1,2 1National Clinical Research Center for Aging and Medicine & National Center for Neurological Disorders, Huashan Hospital, Fudan University, Shanghai, China, 2Department of Neurology, Huashan Hospital, Fudan University, Shanghai, China Author contributions Study concept and design: QZ, CZ, YG, JL and XM; acquisition, analysis, or interpretation of data: all authors; drafting of the manuscript: JL and XM; critical revision of the manuscript for important intellectual content: QZ, CZ, YG, TZY, HZ, ZX, ML, ZJ, JW, LC, LZ, JG, XL, WB, PW, and DD. Funding This work was supported by grants from the National Natural Science Foundation of China (81971641, 82071200, 82272039, and 82021002), the STI2030-Major Project (2022ZD0211600), the Clinical Research Plan of Shanghai Hospital Development Center (SHDC2020CR1038B, SHDC2020CR4007), the National Key R&D Program of China (2022YFC2009902, 2022YFC2009900), and Medical Innovation Research Project of Shanghai Science and Technology Commission (21Y11903300). Availability of data and materials Fully anonymized data will be shared upon request from qualified investigators, subject to approval by the China Human Genetic Resources Administration Office. Data transfer will have to comply with the Regulations of the People’s Republic of China. Declarations Ethics approval and consent to participate This study was approved by the Institutional Review Board of Huashan Hospital. Written informed consent was obtained from all participants or legal guardians. Consent for publication Not applicable. 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