
==== Front
Plast Reconstr Surg Glob Open
Plast Reconstr Surg Glob Open
GOX
Plastic and Reconstructive Surgery Global Open
2169-7574
Lippincott Williams & Wilkins Hagerstown, MD

GOX-D-24-00774
00044
10.1097/01.GOX.0001063984.58514.f7
3
PSTM Abstract Supplement
PSTM Top Abstracts/Posters 2024
Piezo Inhibition Prevents and Rescues Scarring by Targeting the Adipocyte to Fibroblast Transition
Griffin Michelle MD 1
Wan Derrick MD 1
Longaker Michael MD, MBA, FACS 1
Stanford University, CA
9 2024
18 9 2024
12 Suppl 33-34Copyright © 2024 The Authors. Published by Wolters Kluwer Health, Inc. on behalf of The American Society of Plastic Surgeons. All rights reserved.
2024
https://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

OPEN-ACCESSTRUE
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pmcBACKGROUND: While studies have suggested that plasticity exists between dermal fibroblast and adipocytes, it remains unknown whether fat contributes to fibrosis. We hypothesized that mature adipocytes directly participate in wound repair via conversion into fibroblasts, and that adipocyte-derived fibroblasts (ADFs) contribute to skin scarring.

METHODS: AdipoqCreERT transgenic driver mice were crossed to R26mTmG reporter mice to generate AdipoqCreERT;ROSA26mTmG mice to perform lineage tracing of mature adipocytes. To achieve local adipocyte ablation AdipoqCre;ROSA26mTmG;R26tm1(HBEGF)Awai mice were generated and wounded, and diphtheria toxin (DT) was injected into the wound base. DT- and vehicle control-treated wounds underwent histologic analysis. Lastly, we performed scRNAseq, Visium gene spatial analysis, and CODEX protein analysis on wounded and unwounded tissue to identify fibrotic ADF subpopulations.

RESULTS: Using our AdipoqCre;ROSA26mTmG adipocyte lineage-tracing model, we identified significantly greater number of adiponectin lineage-positive cells (GFP+) within wounds at post-operative day-14 (POD-14) compared to unwounded skin (*P<0.05, n=12). Compared to typical subcutaneous adipocytes, the GFP+ cells exhibited upregulation of fibroblast markers and downregulation of adipocyte markers. FACS further confirmed that the GFP+ cells were fibroblasts and increased to 10% at POD-14. DT-induced ablation of Adipoq lineage-positive cells exhibited reduced scar thickness and collagen deposition at POD-14 compared to control wounds (*P<0.05, n=12). Using an in-vitro collagen gel system we establish that mechanics alone is sufficient to drive the adipocyte-to-fibroblast conversion. scRNAseq and spatial gene and protein analysis revealed distinct mechanical sensitive subpopulations of ADFs expressing high levels of Piezo1 and Piezo2. We further show that Piezo1 or Piezo2 -inhibition yields regenerative healing by preventing adipocytes’ activation to fibroblasts, in both mouse-wounds using small molecule Piezo inhibitors and Piezo1/Piezo2 genetic knockouts. In addition, Piezo1 inhibition via P1i overcomes skin scarring in a novel human-xenograft-wound model. Lastly, we show that Piezo1 inhibition can was sufficient to induce near-complete wound regeneration of 1- month, 2.5 month and 4 month established mouse scars with return of hair follicles, including full recovery of unwounded-like extracellular architecture, compared to untreated wounds which remained scar-like on histology (n=30).

CONCLUSION: Our findings strongly suggest that mature adipocytes in the skin undergo conversion to pro-fibrotic fibroblasts in response to injury. Importantly, Piezo1 -inhibition induced wound regeneration even in pre-existing established scars, a finding that suggests a role for adipocyte-to-fibroblast transition in wound remodeling, the least-understood phase of wound healing.
