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Sci Rep
Sci Rep
Scientific Reports
2045-2322
Nature Publishing Group UK London

39294188
71815
10.1038/s41598-024-71815-3
Author Correction
Author Correction: Melatonin-mediated mitophagy protects against early brain injury after subarachnoid hemorrhage through inhibition of NLRP3 inflammasome activation
Cao Shenglong
Shrestha Sudeep
Li Jianru
Yu Xiaobo
Chen Jingyin
Yan Feng
Ying Guangyu
Gu Chi
Wang Lin
Chen Gao d-chengao@zju.edu.cn

https://ror.org/059cjpv64 grid.412465.0 Department of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China
18 9 2024
18 9 2024
2024
14 21823© The Author(s) 2024
2024
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pmcCorrection to: Scientific Reports 10.1038/s41598-017-02679-z, published online 25 May 2017

This Article contains errors. Due to errors in figure assembly, the GAPDH western blot panels in Figures 2A, 2B, 3A, 3B, 4A, 5A, and 5B do not correspond to the same experimental replicates as the proteins of interest shown in the respective figures. Multiple replicates for each experimental set were run alongside on the same SDS-PAGE; the GAPDH bands were incorrectly selected from adjacent replicates rather than from the same experimental set as the proteins of interest.

The corrected Figure 2 and its accompanying legend appear below.Figure 2 Effect of melatonin treatment on autophagy. (A) The Western blot relative band density of LC3; n = 6 per group. (B) The Western blot relative band density of Atg5; n = 6 per group. The cropped bands had been run under the same experimental conditions. (C) Representative micrographs showing double immunofluorescence labeling of Beclin-1 with NeuN (neuronal marker), GFAP (astrocyte marker) and Iba-1 (microglia marker) in the ipsilateral basal cortex at 24 h after SAH. White arrows indicated double-labeled cells. Scale bar = 50 μm. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.

The corrected Figure 3 and its accompanying legend appear below.Figure 3 Effect of melatonin treatment on mitophagy and ROS generation. (A) The Western blot relative band density of Parkin; n = 6 per group. (B) The Western blot relative band density of PINK1; n = 6 per group. The cropped bands had been run under the same experimental conditions. (C) Ultrastructural changes in mitochondria in the ipsilateral basal cortex at 24 h after SAH induction. (D) The quantification of ROS levels in the ipsilateral basal cortex at 24 h after SAH; n = 6 per group. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.

The corrected Figure 4 and its accompanying legend appear below.Figure 4 Melatonin treatment inhibits NLRP3 inflammasome activation. (A) Representative Western blot bands of NLRP3, ASC, and cleaved caspase-1. (B–D) The relative band densities of NLRP3, ASC, and cleaved caspase-1; n = 6 per group. The cropped bands had been run under the same experimental conditions. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.

The corrected Figure 5 and its accompanying legend appear below.Figure 5 Melatonin treatment inhibits microglia activation and pro-inflammatory cytokine secretion. (A) The Western blot relative band density of IL-1β; n = 6 per group. (B) The Western blot relative band density of IL-18; n = 6 per group. The cropped bands had been run under the same experimental conditions. (C) Upper: representative micrographs showing microglial activation in the lesion area at 24 h after SAH, with immunofluorescence for Iba-1. Upper scale bar = 50 μm, lower scale bar = 10 μm. Lower: quantification of Iba-1-positive cells. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.

In addition, there is an error in Figure 6A. Due to an error during figure assembly, an image from the SAH(Mel + 3-MA) group was selected for the SAH(-) group. The corrected Figure 6 and its accompanying legend appear below.Figure 6 Effect of melatonin treatment on neuronal cell death. (A) Representative micrographs showing TUNEL, PANT and Fluoro-Jade C staining in the ipsilateral basal cortex at 24 h after SAH. Scale bar = 50 μm. (B–D) The quantification of TUNEL-positive cells, PANT-positive cells, and Fluoro-Jade C-positive cells. *P < 0.05 versus sham group, #P < 0.05 versus SAH + vehicle group, and &P < 0.05 versus SAH + Mel group.
