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Proc Natl Acad Sci U S A
Proc Natl Acad Sci U S A
PNAS
Proceedings of the National Academy of Sciences of the United States of America
0027-8424
1091-6490
National Academy of Sciences

202416561
10.1073/pnas.2416561121
correctCorrection428
438
Correction
Correction for Bao et al., Sugar status in preexisting leaves determines systemic stomatal development within newly developing leaves
6 9 2024
10 9 2024
6 9 2024
121 37 e24165611212024
https://creativecommons.org/licenses/by-nc-nd/4.0/ This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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pmcPlant Biology Correction for “Sugar status in preexisting leaves determines systemic stomatal development within newly developing leaves,” by Qin-Xin Bao, Xin-Rong Mu, Chen Tong, Cong Li, Wen-Zhe Tao, Sheng-Ting Zhao, Yu-xin Liu, Wan-Ni Wang, Yu-ting Wei, Fu-Huan Yu, Jing-wen Wang, Zhi-Lan Sun, Bing-Ling Fan, Jia Sun, Chen Wang, Gary J. Loake, and Lai-Sheng Meng, which published June 5, 2023; 10.1073/pnas.2302854120 (Proc. Natl. Acad. Sci. U.S.A. 120, e2302854120).

The authors note that published Fig. 5 and SI Appendix, Fig. S1 appeared incorrectly. The corrected Fig. 5 and its legend appear below. The online version has been corrected, and the SI Appendix has been corrected online.

Fig. 5. SUC2 expression in mature cotyledons is sufficient to promote stomatal formation in young leaves. (A) Images illustrating the 3rd or 4th leaves (as sink leaves) harvested from either 16-, 18-, or 20-d-old ER::SUC2- 3×FLAG/suc2-5 seedlings. Mature cotyledons (as source leaves) (but not other organs) of 15-d-old ER::SUC2-3×FLAG/suc2-5 seedlings were treated with 20 μM estradiol for 1 h or mock treated. Subsequently, the 3rd or 4th leaves of these seedlings were photographed. The size of these 3rd or 4th leaves was not visibly different between the estradiol or mock treated lines. iER represents an inducible transgene ER::SUC2- 3×FLAG/suc2-5. (B) Images illustrating abaxial epidermal stomata of the given leaves in A. (Bars, 50 μm.) (C) Images illustrating the abundance of SUC2 in mature cotyledons of either 16-, 18-, or 20-d-old ER::SUC2-3×FLAG/suc2-5 seedlings. SUC2 accumulation was detected using an anti-FLAG antibody. Tubulin was used as a loading control. Similar findings were found in at least three biological repeats. (D) Images illustrating the abundance of SUC2 in 16-d-old ER::SUC2-3×FLAG/suc2-5 seedlings. Lane 1 represents cotyledons treated by estradiol (positive control), lane 2 represents the 3rd/4th leaf following cotyledons treated by estradiol, and lane 3 represents the 3rd/4th leaf following cotyledons treated by mock (negative control). SUC2 accumulation was detected using an anti-FLAG antibody. Tubulin was used as a loading control. Similar findings were found in at least three biological repeats. (E) Bar graph illustrating differential expression of SUC2 in cotyledons of either 16-, 18-, or 20-d- old ER::SUC2-3×FLAG/suc2-5 seedlings. Quantification at 16 d following mock treatment was set as 1 in qPCR. (F) Bar graph illustrating Suc content in phloem exudates from cotyledons of either 16-, 18-, or 20-d-old ER::SUC2-3×FLAG/suc2-5 seedlings. (G) Bar graph illustrating the given Suc contents in A. (H) Bar graph illustrating the quantification of the given abaxial stomata in B. Student’s t test (**P < 0.01; ***P < 0.001). Error bars represent SD (n = 3).
