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RSC Adv
RSC Adv
RA
RSCACL
RSC Advances
2046-2069
The Royal Society of Chemistry

d4ra03725a
10.1039/d4ra03725a
Chemistry
Suzuki–Miyaura cross-coupling of unprotected ortho-bromoanilines with benzyl, alkyl, aryl, alkenyl and heteroaromatic boronic esters†
† Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4ra03725a

Lubaev Alexandra E. a
https://orcid.org/0000-0003-4984-8435
Marvin Christopher C. b
https://orcid.org/0000-0001-9596-263X
Dombrowski Amanda W. b
https://orcid.org/0009-0004-5553-6424
Qureshi Zafar b
a Department of Chemistry and Biochemistry, Baylor University Waco Texas 76706 USA
b AbbVie, Inc. 1 N. Waukegan Road North Chicago Illinois 60064 USA zafar.qureshi@abbvie.com

16 9 2024
12 9 2024
16 9 2024
14 40 2918429188
20 5 2024
14 8 2024
This journal is © The Royal Society of Chemistry
2024
The Royal Society of Chemistry
https://creativecommons.org/licenses/by-nc/3.0/ This article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.
A Suzuki–Miyaura cross-coupling reaction was developed on unprotected ortho-bromoanilines. This operationally simple reaction was developed for the diversification of glucocorticoid receptor modulators (GRMs), showed compatibility to various boronic esters featuring unique functionalities, and was demonstrated on a gram scale.

An efficient Suzuki–Miyuara reaction was developed on unprotected ortho-bromoanilines providing good to excellent yields on a wide variety of substrates. The robustness of the reaction was showcased on gram scale.

AbbVie 10.13039/100006483 Unassigned pubstatusPaginated Article
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pmcThe widely used Suzuki–Miyaura cross-coupling reaction employs the use of a palladium catalyst to generate carbon–carbon bonds between an organohalide or triflate and an organoboron nucleophile.1 Its wide use can be attributed to its mild reaction conditions, low toxicity, and commercial availability of starting materials.2 Of note, is the application towards natural product synthesis, pharmaceuticals, and fine chemical industries.3Ortho-substituted anilines are a key structural element in several pharmacologically active compounds. Examples include inhibitors of fatty acid amide hydrolase (FAAH), phosphodiesterase-4 (PDE4), compounds for the prevention of nonalcoholic fatty liver disease (NAFLD), and angiotensin II receptor antagonists (Fig. 1a).4 Although the Suzuki–Miyaura reaction has been widely utilized, substrates with unprotected ortho-anilines are less common. Particularly challenging are sp2–sp3 couplings. And thus, the development of a cross-coupling method focusing on unprotected ortho-bromoanilines is of wide interest to the broader chemistry community. Reported methods for this type of coupling typically require protection of the free amine.5 Alternatively, methods exist with direct reactivity on various 2-haloanilines but with limitations on the substrates investigated and functionalities that can be tolerated.6 Our work focuses on expanding the scope of the reactivity of ortho-bromoanilines, with a focus on developing an operationally simple method (Fig. 1b).

Fig. 1 (a) Examples of pharmacologically active compounds containing ortho-substituted anilines. (b) Our work on coupling unprotected ortho-bromoanilines with benzyl, alkyl, aryl, alkenyl and heteroaromatic boronic esters.

As a part of a program to identify glucocorticoid receptor modulators (GRMs),7 the Suzuki cross-coupling of 1a was attempted as the diversification step for the preparation of a compound library.8 Using Pd(dppf)Cl2 as the catalyst with K2CO3 as base with boronate 2a in 10 : 1 dioxane/H2O at 90 °C, desired product 3a was isolated in 11% yield (Fig. 2). The low isolated yield was consistent with conversion by HPLC.

Fig. 2 Initial attempt with GRM substrate.

Since this result suggested a lack of catalyst turnover, a high throughput screen was conducted using ChemBeads9 to identify an optimal catalyst/ligand/base system with the goal of improving the yield. The best conditions identified from the screen used the preformed CataCXium A palladacycle10 with Cs2CO3 as the base in dioxane/H2O at 80 °C. These results were repeated on a 0.1 mmol scale at a 0.1 M concentration of 1a with 2 equiv. of boronate to give 51% yield of the desired product by NMR (Table 1, entry 1). Switching the solvent from dioxane to EtOAc or PhMe did not give any appreciable increases in yield (entries 2 and 3). The biggest increase in yield came from switching to 2-MeTHF as the solvent. The product was isolated in 95% yield (entry 4). To confirm the unique reactivity of the CataCXium A palladacycle, these optimized conditions were tested using several additional catalysts, all yielding little to no product (entries 5–10). Decreasing the catalyst loading to 5 mol% also had a detrimental effect on the yield (entry 11). Decreasing the boronate loading to 1.5 equiv. and increasing the reaction concentration to 0.3 M gave 91% yield. We chose to move forward with these conditions since they would be most amenable to scaling up the reaction.

Reaction optimizationa

	
Entry	Catalyst	Solvent (conc.)	2a equiv.	NMR yieldb		
1	CataCXium A Pd G3	Dioxanes (0.1)	2	51%		
2	CataCXium A Pd G3	EtOAc (0.1)	2	56%	
3	CataCXium A Pd G3	PhMe (0.1)	2	56%	
4	CataCXium A Pd G3	2-MeTHF (0.1)	2	95%c	
5	Pd(Amphos)Cl2	2-MeTHF (0.1)	2	22%	
6	SPhos Pd G4	2-MeTHF (0.1)	2	0%	
7	Pd(OAc)2	2-MeTHF (0.1)	2	0%	
8	Pd(dbe)3	2-MeTHF (0.1)	2	0%	
9	XPhos Pd G3	2-MeTHF (0.1)	2	0%	
10	(PPh3)2PdCl2	2-MeTHF (0.1)	2	46%	
11d	CataCXium A Pd G3	2-MeTHF (0.1)	2	42%	
12	CataCXium A Pd G3	2-MeTHF (0.1)	1.5	91%c	
a All reactions were carried out under an atmosphere of N2 using 0.1 mmol of starting material.

b NMR yields were calculated on the crude reaction mixtures, using 0.05 mmol of mesitylene as an internal standard.

c Isolated yield.

d 5 mol% catalyst used.

The final optimized conditions were tested on a gram scale on substrate 1a with the desired product obtained in 80% yield. We also applied these conditions to two analogs, one bearing an electron rich aromatic ring and the other bearing an electron deficient aromatic ring. Our optimized conditions produced both products in excellent isolated yields (Fig. 3).

Fig. 3 Scale-up and limited scope of GRM substrate1.

To further explore our reaction, we chose to continue with a more generic substrate (1). To accommodate a wide variety of substrates, minor modifications were made to the optimized conditions including decreasing the catalyst loading to 5 mol%, decreasing the temperature to 70 °C and decreasing the concentration to 0.1 M (Fig. 4). With these modified conditions, substrate 3d, was isolated in 91% yield on a 0.5 mmol scale. Boronates containing electronically neutral and electron-donating substituents were compatible with these conditions and isolated in synthetically useful yields. An alkyl boronate with unprotected alcohol was also amenable in the reaction giving 3g in 82% yield, however sterically hindered alkyl boronates isopropylboronic acid pinacol ester and cyclohexylboronic acid pinacol ester did not give the desired product. Arylated substrates (3h–3n) were compatible with these conditions with the products isolated in up to 97% yield. Ketone containing compound 3h reacted smoothly in 80% yield. Aryl chloride and free alcohol substrates reacted in moderate yields (3i–3j). Both electron-rich and electron-poor aryl boronates afforded the coupled products in high yield (3k–3l). The nitrated substrate 3l precipitated out of solution after completion of the reaction and simple filtration led to the isolation of pure product without the need for chromatographic purification. Substrates 3m and 3n provide differentially protected anilines to aid in downstream synthesis. Alkenyl substrates also reacted smoothly giving good yields for the coupled products 3o–3u. The reaction was tolerant of benzoxazole, furan, isoquinoline, pyrazole, carbazole, indole and pyridine motifs (3v–3ab). Ortho-bromoanilines with different substituents were also tested. Methoxy and fluorinated analogs 3ac–3af were produced in good yields. Alternative electron withdrawing groups such as nitrile, nitro and sulfone were also tolerated in the reaction (3ag–3ai). Finally, the reaction was also successful in coupling an ortho/ortho’ substituted analog giving product 3aj in 40% yield.

Fig. 4 Substrate scope1.

In conclusion, we identified CataXCium A Pd G3 as a uniquely effective catalyst system for Suzuki–Miyaura cross-couplings on GRM substrate 1a. This method facilitates coupling of benzyl, alkyl, aryl, alkenyl, and heteroaromatic substituents on model substrate 1. Diverse functional group tolerability has been exemplified with support of electron-rich and electron-poor groups, halogenated aryls, protected and unprotected amines, and various heterocycles.

Data availability

The data supporting this article have been included as part of the ESI.†

Conflicts of interest

There are no conflicts to declare.

Supplementary Material

RA-014-D4RA03725A-s001

Authors CCM, AWD and ZQ are employees of AbbVie. AEL is a student in the Department of Chemistry and Biochemistry at Baylor University. The design, study conduct, and financial support for this research were provided by AbbVie. AbbVie participated in the interpretation of data, review and approval of the publication. The authors thank the following AbbVie scientists for their technical support of this project: Leena Bhatt, Rick Yarbrough and Ian Marsden. The authors also thank Chau-Wen Chou from the Proteomics and Mass Spectrometry Core Facility at the University of Georgia for high resolution mass spectrometry analysis. AbbVie paid for HRMS analysis on a per sample basis.
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