
==== Front
bioRxiv
BIORXIV
bioRxiv
2692-8205
Cold Spring Harbor Laboratory

10.1101/2024.09.01.610697
preprint
1
Article
Improved method for detecting protein-protein interactions using proximity ligation assay
Tower Zach http://orcid.org/0009-0008-4067-6271

Chang Hao http://orcid.org/0000-0002-4208-9088

02 9 2024
2024.09.01.610697https://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
http://biorxiv.org/lookup/doi/10.1101/2024.09.01.610697
nihpp-2024.09.01.610697.pdf
ABSTRACT

Proximity ligation assay has been widely used to detect protein-protein interaction in cells and tissues. While with great sensitivity, its specificity was often neglected. Here, we report the existence of varying levels of false positives observed with this assay and provide suggestions to minimize false positives for more accurate detection of protein-protein interactions, especially for membrane proteins.
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pmc
