
==== Front
Food Waterborne Parasitol
Food Waterborne Parasitol
Food and Waterborne Parasitology
2405-6766
Elsevier

S2405-6766(24)00023-4
10.1016/j.fawpar.2024.e00241
e00241
Research Article
Prevalence of Entamoeba histolytica/dispar and Giardia lamblia infections and their associated factors among schoolchildren in the Amhara region, Northwest Ethiopia
Hailu Tadesse tadessehailu89@gmail.com
⁎
Alemu Getaneh
Department of Medical Laboratory Science, College of Medicine and Health Sciences, Bahir Dar University, P. O. Box: 79, Ethiopia
⁎ Corresponding author. tadessehailu89@gmail.com
13 8 2024
9 2024
13 8 2024
36 e0024124 6 2024
9 8 2024
12 8 2024
© 2024 The Authors. Published by Elsevier Inc. on behalf of International Association of Food and Waterborne Parasitology.
2024

https://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Globally, intestinal protozoa E. histolytica/dispar and Giardia lamblia are the cause of amoebiasis and giardiasis, respectively. Despite their important medical importance and common occurrence in Ethiopia, they are minimally addressed in terms of their prevalence, sensitive diagnostic methods, and associated risk factors. Infections with E. histolytica/dispar and G. lamblia are often misdiagnosed and underreported in impoverished countries. Thus, the purpose of this study was to ascertain the prevalence of Giardia lamblia and E. histolytica/dispar infections as well as related variables among schoolchildren in the Amhara region. A cross-sectional study was conducted among 844 schoolchildren in the Amhara region from April to December 2019. A stool sample was collected from each study participant and processed via the formol ether concentration technique (FECT) and spontaneous tube sedimentation techniques (STST). Data were entered in EpiData and analysed by SPSS statistical software. The prevalence of E. histolytica and G. lamblia infections using each diagnostic method and composite reference was determined by descriptive statistics. The association of risk factors with E. histolytica/dispar and G. lamblia infections was analysed by logistic regression and variables with p < 0.05 were considered to have statistical significance. From the total, 243 (28.8%) schoolchildren were found to be infected by at least one of E. histolytica/dispar or G. lamblia. The prevalence of E. histolytica/dispar and G. lamblia infections was 201 (23.8%) and 62 (7.3%), respectively. The co-infection prevalence with both E. histolytica/dispar and G. lamblia was 22 (2.6%). The sensitivity (78.6%) and negative predictive value of STST (19.6%) were higher than FECT sensitivity (65.4%) and negative predictive value (13.1%). Children in 10–14 years of age (AOR = 1.66;95%CI: 1.16–2.38), lived in the rural (AOR = 1.97;95%CI: 1.12–3.49), used latrine improperly (AOR = 1.49;95%CI: 1.04–2.13), did not wash hands before meal (AOR = 2.10; 95%CI:1.08–4.10), and after latrine (AOR = 1.51;95%CI: 1.05–2.19), ate unwashed raw vegetables (AOR = 1.85;95%CI:1.26–2.70), and played with soil (AOR = 1.48;95%CI:1.06–2.06) were associated with E. histolytica/dispar and G. lamblia infection. These findings revealed high prevalence of E. histolytica/dispar and G. lamblia infections was high in the Amhara region. Therefore, proper implementation of water, sanitation and hygiene should be advocated at the community and school levels to mitigate the disease burden.

Keywords

Prevalence
Schoolchildren
Protozoa
Entamoeba histolytica
Giardia lamblia
Amhara region
Ethiopia
==== Body
pmc1 Introduction

Intestinal protozoa infections caused by Entamoeba histolytica and Giardia lamblia are common worldwide, especially in developing countries. Ninety percent of those affected are carriers of amoebiasis without any symptoms, according to Stanley (2003). The silent infection of E. histolytica seems to be more dangerous than the symptomatic illness because neglect might lead to severe amoebic dysentery. Trophozoites in the intestinal lumen (a non-invasive infection) usually only affect carriers who do not exhibit any symptoms (Zaki, 2022). The California Department of Public Health estimated that 411 cases of amoebiasis had been documented in this state alone in 2007 and that the prevalence of E. histolytica infection nationwide was roughly 4% (California MSR, 2007). Protozoa of the genus G. lamblia are comparable to this and are capable of infecting a wide range of animals. The prevalence of G. lamblia is at least 30% in poorer nations, compared to the 2%–5% range in industrialized nations (Tektook et al., 2019).

Consuming food or water contaminated with the parasites' is the commonly route to transmit infections with E. histolytica and G. lamblia. They can also be directly transferred from person to person by polluted water, fresh produce, soil, and residing in endemic areas (Mama and Alemu, 2016; Callixte et al., 2019). Poor nutrition, a lack of health services, and poverty are key risk factors for the high occurrence of E. histolytica and G. lamblia infections, according to studies by Bahrami et al. (2018) and Callixte et al. (2019).

Most amoebiasis and giardiasis diagnoses are obtained from stool samples by molecular, serological, or parasitological techniques (Saidin et al., 2019; Hooshyar et al., 2019). The formol ether concentration (FECT) and spontaneous tube sedimentation technique (STST) are two alternative parasitological diagnostic methods for intestinal parasitic infections (Allam et al., 2021; Tello et al., 2012). While STST is inexpensive, easy to use in remote areas, and has a better sensitivity, it is not widely used as a routine diagnostic method for intestinal parasitic infections.

Countries like Ethiopia deal with a variety of public health challenges, such as the prevalence of intestinal parasitic infections and the dearth of reliable diagnostic instruments. Increasing the detection rates of intestinal parasites in people living in severe poverty is an urgent need. Nevertheless, in order for a diagnostic technique to be successful in a low-income society, it must be highly sensitive and reasonably priced. In clinical settings, intestinal parasite infection is diagnosed via direct saline microscopy. As a result, infections with G. lamblia and E. histolytica/dispar are underdiagnosed and underreported nationwide. Therefore, it's essential to search for new user-friendly, cost-effective, and sensitive diagnostic methods to identify giardiasis and amoebiasis. Additionally, the advantages of establishing water, sanitation, and hygiene (WASH) in areas where G. lamblia and E. histolytica/dispar are endemic are poorly understood. This study attempted to examine the field performance of FECT and STST in identifying both protozoan species, as well as the prevalence and variables related to infection with G. lamblia and E. histolytica/dispar among schoolchildren in the Amhara region.

2 Materials and methods

2.1 Study design, period and area

From April to December 2019, schoolchildren in the Amhara region participated in a school-based cross-sectional study to find out how common illnesses with G. lamblia and E. amoeba/dispar were. The region's yearly mean temperature ranges from 15 °C to 21 °C. There is 1145 mm of rain on average every year. In the Amhara region, June to August and October to May are the wettest and driest months, respectively.

2.2 Sample size and sampling technique

To calculate the sample size, a 95% confidence interval (CI), a 5% margin of error, p = 50%, the design effect 2 and 10% of the non-response were used since there was no previous prevalence research on amoebiasis or giardiasis in the Amhara region. The total sample size was 844.

The districts, primary schools, and schoolchildren in the Amhara region were selected by using a multistage sampling technique. Seven districts (Toledere, Gosamen, Dangila Zuria, Fogera, Bahir Dar, Metema, and Jawi) were randomly selected. Within the seven districts, thirteen primary schools (Bededo, Korke, Wonka, Gultabeshikan, Dokemit, Nora, Zege, Robit, Aftit, Azobahir, and Workmeda) were also selected by a simple random sampling technique. The study comprised 6–14-year-old students whose parents or guardians volunteered and gave their consent. Students who had taken anti-protozoa medications within the three months preceding the data collection period were not allowed to participate in the research. Each primary school's study courses were distributed proportionately to the total number of students enrolled in the institution. Lastly, students who were willing to participate in the study were chosen by a rigorous random sampling process.

2.3 Data collection

Parents provided demographic and environmental data via questionnaires that were gathered by trained health workers. Stool cups were used to collect fresh stool samples from research participants. The samples were then brought to the medical facility and processed using modified FECT and STST to identify G. lamblia and E. histolytica/dispar.

Modified FECT: Approximately 0.5 g of stool, 1 ml of ethyl acetate and 1.5 ml of 10% formalin were added to the collection tube and well mixed. The mixture was thoroughly combined, and was spun at 123 g, and 3 min. One drop of the sediment was placed on a microscope slide, covered with a cover slip, and examined under a microscope to look for G. lamblia and E. histolytica/dispar (Young et al., 1979; Jember et al., 2022).

STST: About 10 ml of saline solution and 3 g of stool samples were mixed and filtered in a 50 ml Falcon tube. The Falcon tube was tightly sealed, loaded with more saline solution, vigorously shaken and left to stand for 45 min. One drop of sediment from the bottom was put on the microscope slide, coved with a cover slip, and inspected under a microscope to check for G. lamblia and E. histolytica/dispar cysts and trophozoites (Tello et al., 2012; Jember et al., 2022).

2.4 Performance evaluation of diagnostic methods

The performance of FECT and STST was evaluated against the composite reference using the following metrics: sensitivity (SN), specificity (SP), positive predictive value (PPV), and negative predictive value (NPV). This is because there was no available “Gold” standard diagnostic method for the detection of E. histolytica/dispar and G. lamblia. The diagnostic agreement of the techniques was evaluated using the Kappa value (McHugh, 2012).

2.5 Data quality assurance and data analysis

Laboratory staff and health officers received training on how to collect data prior to data collection. During sample collection, the quantity of stool samples was verified and stool cups were appropriately labelled. In <1 h after collection, the sample was delivered to the laboratory of the adjacent medical facility. For every laboratory test, a standard operating protocol was followed to. Microscopic examination of stool slides was performed by two laboratory technologists independently in order to remove observer bias. The findings of their observations were noted on different sheets for future comparison. The inconsistent outcomes were re-examined. Generally, data quality was checked during the pre-, analytical, and post-analytical phases.

The Statistical Package for Social Sciences (SPSS) version 23 was used to analyze the data once they were entered into the EpiData program. A combination of descriptive statistics and Chi-square was used to determine the overall prevalence of giardiasis and amoebiasis. Both univariate and multivariate logistic regression models were used to calculate the degree of correlation between the dependent and independent variables. In the multivariate analysis, variables with p < 0.05 were deemed statistically significant.

2.6 Ethical considerations

The Ethical Review Committee at Science College, Bahir Dar University granted ethical approval. The Amhara Public Health Institute, the Amhara National Regional Education Bureau, and the zonal education offices provided supportive correspondence. Letters of authorization were obtained from every elementary school and district education office that were chosen. Written informed consent was acquired from schoolchildren's parents or guardians. Individuals in the study who tested positive for any intestinal parasite were referred to medical professionals for treatment.

3 Results

3.1 Socio-demographic characteristics of the study participants

In all, 844 students took part in the research. Participants in the study ranged in age from 6 to 14 years old, with a mean age of 10.3. Of the 591 study participants, the majority (70.0%) were in the age range of 10 to 14 years. There were more men 436 (51.7%) than women among the participants. The majority of research participants, 745 (88.3%), 683 (80.9%), 735 (87.1%), and 573 (67.9%), were educated to a formal level, had five to nine family members, resided in rural areas, and earned >1000 Birr per month for their families (Table 1).Table 1 Socio-demographic characteristics of schoolchildren with their E. histolytica/dispar and G. lamblia infections in Amhara region, 2019.

Table 1Characteristics	Total n (%)	E. histolytica/dispar and G. lamblia	χ2/ p-value	
Positive n (%)	Negative n (%)	
Age (year)	6–9	253 (30.0)	54 (21.3)	199 (78.7)	10.06/0.001	
10–14	591 (70.0)	189 (32.0)	402 (68.0)	
Sex	M	436 (51.7)	147 (33.7)	289 (66.3)	9.86/0.001	
F	408 (48.3)	96 (23.5)	312 (76.5)	
Residence	Rural	745 (88.3)	227 (30.5)	518 (69.5)	7.52/0.003	
Urban	99 (11.7)	16 (16.2)	83 (83.8)	
Family size	1–4	161 (19.1)	47 (29.2)	114 (70.8)	0.01/0.50	
5–9	683 (80.9)	196 (28.7)	487 (71.3)	
Family educational status	Illiterate	109 (12.9)	35 (32.1)	74 (67.9)	062/0.24	
Educated	735 (87.1)	208 (28.3)	527 (71.7)	
Family income in birr	<1000	271 (32.1)	76 (28.0)	195 (72.0)	0.15, 0.38	
≥1000	573 (67.9)	167 (29.1)	406 (70.9)	
Total		844 (100)	243 (28.8)	600 (71.1)		

3.2 Prevalence of E. histolytica/dispar and G. lamblia

From the total, 243 (28.8%) schoolchildren were infected at least by one of the E. histolytica/dispar or G. lamblia parasites. High prevalence of E. histolytica/dispar and G. lamblia (189 (32.0%), 147 (33.7%), and 227 (30.5%) were obtained in the respective 10–14 age groups, male participants, and rural dwellers. The prevalence rate of E. histolytica/dispar and G. lamblia co-infections was 22 (2.6%) (Table 1).

Using a combination of FECT and STST, the prevalence of G. lamblia and E. histolytica/dispar was 62 (7.3%) and 201 (23.8%), respectively. Using FECT and STST, the corresponding prevalences of E. histolytica/dispar were 141 (16.7%) and 165 (19.5%). The FECT and STST detection rates for G. lamblia infection were 34 (4.0%) and 47 (5.6%), respectively. When it came to the identification of G. lamblia and E. histolytica/dispar, there was a significant difference (p < 0.01) between FECT and STST (Table 2).Table 2 The detection of E. histolytica/dispar and G. lamblia by formol-ethyl acetate test, spontaneous tube sedimentation test and combined diagnostic methods among primary schoolchildren in Amhara region, 2019.

Table 2Characteristics	Total (n)	FECT [n (%)]	STST [n (%)]	Combined total n [(%)]	
E. h/d	G. l	E. h/d	G. l	E. h/d	G. l	
Age (year)	6–9	253	35 (13.8)	7 (2.8)	32 (12.6)	14 (5.5)	45 (17.9)	16 (6.3)	
10–14	591	106 (17.9)	27 (4.6)	133 (22.5)	33 (5.6)	156 (26.4)	46 (7.8)	
Sex	M	436	86 (19.7)	24 (5.5)	88 (20.2)	32 (7.3)	118 (27.1)	40 (9.2)	
F	408	55 (13.5)	10 (2.5)	77 (18.9)	15 (3.7)	83 (20.3)	22 (5.4)	
Residence	Rural	745	131 (17.6)	31 (4.2)	152 (20.4)	44 (5.9)	187 (25.1)	57 (7.7)	
Urban	99	10 (10.1)	3 (3.0)	13 (13.1)	3 (3.0)	14 (14.1)	5 (5.1)	
Total	844	141 (16.7)	34 (4.0)	165 (19.5)	47 (5.6)	201 (23.8)	62 (7.3)	
FECT = formol-ethyl acetate, STST = spontaneous tube sedimentation test, E. h/d = E. histolytica/dispar, G. l = G. lamblia.

3.3 Performance of E. histolytica/dispar and G. lamblia diagnostic methods

High sensitivity (78.6%) and negative predictive value (19.6%) in at least one of amoebiasis or giardiasis detection were obtained by STST by utilizing the combination of FECT and STST procedures as composite references. STST yielded higher amoebiasis detection sensitivity (80.1%) and negative predictive value (20.8%). In the detection of G. lamblia, STST yielded improved sensitivity (74.2%), specificity (99.9%), and negative predictive value (16.9%). The agreements of STSTS were almost perfect in both amoebiasis and giardiasis detections. FECT also had a substantial agreement with amoebiasis and giardiasis detections (Table 3).Table 3 The performance of formol-ethyl acetate test, spontaneous tube sedimentation test in E. histolytica/dispar and G. lamblia detection compared to the composite reference, 2019.

Table 3Methods	Composite reference to amoebiasis or giardiasis	
	Pos
(N)	Neg
(N)	SN (95%CI)	SP (95%CI)	PPV (95%CI)	NPV (95%CI)	K value	
FECT	159	7	65.4 (59.1–71.4)	98.8 (97.6–99.5)	99.91 (99.80–99.96)	13.1 (11.2–15.2)	0.710	
	84	596						
STST	191	7	78.6 (72.9–83.6)	98.8 (97.6–99.5)	99.92 (99.84–99.96)	19.6 (16.0–23.6)	0.820	
	52	594						
	Composite reference to amoebiasis	
FECT	140	1	69.7 (62.8–75.9)	99.8 (99.1–100.0)	99.99 (99.92–100.0)	14.8 (12.3–17.6)	0.774	
	61	642						
STST	161	4	80.1 (73.9–95.4)	99.4 (98.4–99.8)	99.96 (99.89–99.98	20.8 (16.6–25.8)	0.847	
	40	639						
	Composite reference to giardiasis	
FECT	32	2	51.6 (38.6–64.5)	99.7 (99.1–100.0)	99.87 (99.89–99.99)	9.8 (7.7–12.3)	0.648	
	30	780						
STST	46	1	74.2 (61.5–84.4)	99.9 (99.3–100.0)	99.99 (99.94–100.0)	16.9 (11.8–23.7)	0.834	
	16	781						
Pos (N) = positive (number), Neg (N) = negative (number), SN = sensitivity, CI = confidence interval, SP = specificity, PPV = positive predictive value, NPV = negative predictive value.

3.4 Factors associated with E. histolytica/dispar and G. lamblia infections

The probability of acquiring G. lamblia and E. histolytica/dispar infections was 1.66 times (AOR = 1.66;95%CI: 1.16–2.38) higher in children between the ages of 10 and 14. Compared to children in urban regions, those in rural areas had 1.97 times (AOR = 1.97;95%CI: 1.12–3.49) higher risk of acquiring G. lamblia and E. histolytica/dispar infections. Similarly, compared to their peers, children who used the latrine inappropriately had a 1.49-fold (AOR = 1.49;95%CI: 1.04–2.13) higher risk of contracting G. lamblia and E. histolytica infections. In a similar way, schoolchildren who were unable to wash their hands prior to meals had a 2.10 (95% CI: 1.08–4.10) increased risk of infection with G. lamblia and E. histolytica/dispar compared to those who could. Similarly, children who could not wash their hands after using the restroom were 1.51 times (AOR = 1.51;95%: 1.05–2.19) more likely to be infected with G. lamblia and E. histolytica/dispar than those who could. Children who consumed raw, unwashed veggies were 1.85 times (AOR = 1.85;95%CI:1.26–2.70) more likely to become sick than children who did not. Children who played in soil had 1.48 times higher infection rates than those who did not (AOR = 1.48;95%CI:1.06–2.06) (Table 4).Table 4 Univariate and multivariate analyses of factors associated with E. histolytica/dispar and G. lamblia infections among schoolchildren in the Amhara region, 2019.

Table 4Variables	Number (n)	COR (95%CI)	P-value	AOR (95%CI)	P-value	
Pos	Neg	
Age	6–9	54	199	1				
10–14	189	402	1.73 (1.23–2.45)	0.002	1.66 (1.16–2.38)	0.006	
Sex	M	147	290	1.64(1.21–2.22)	0.001	1.28 (0.92–1.78)	0.139	
F	96	311	1				
Residence	Rural	227	518	2.27 (1.30–3.97)	0.004	1.97 (1.12–3.49)	0.019	
Urban	16	83	1				
Proper utilization of latrine	No	75	124	1.72 (1.23–2.40)	0.002	1.49 (1.04–2.13)	0.028	
Yes	168	477	1				
Handwashing before meal	No	21	25	2.18 (1.20–3.97)	0.010	2.10 (1.08–4.10)	0.029	
Yes	222	576	1				
Handwashing after latrine	No	82	150	1.53 (1.11–2.12)	0.01	1.51 (1.05–2.19)	0.028	
	Yes	161	451	1				
Eating raw vegetables	Yes	191	416	1.63 (1.15–2.32)	0.006	1.85 (1.26–2.70)	0.002	
	No	52	185	1				
Play with soil	Yes	155	303	1.73 (1.28–2.35)	<0.001	1.48 (1.06–2.06)	0.021	
	No	88	298	1				
COR = crude odds ratio, AOR = adjusted odds ratio.

4 Discussion

In the present study, 28.8% of schoolchildren were infected at least by one of the E. histolytica/dispar or G. lamblia parasites. The current results are in agreement with a previous 25.8% prevalence finding in Africa (Hajissa et al., 2022a, Hajissa et al., 2022b), but higher than previous findings of 20% in northwest Ethiopia (Hailu and Ayele, 2021) and 13.64% in Iraq (Jaeffer, 2011). In the current study, the prevalence of E. histolytica/dispar was 23.8%, which is higher than prior findings of 18.1% in northwest Ethiopia (Hailu and Ayele, 2021), 10.9% in northern Ethiopia (Yeshitila et al., 2020), 13.17% in southern Ethiopia (Abate et al., 2023), 16.7% in Ecuador (Tapia-Veloz et al., 2023), and 7.7% in Nepal (Gupta et al., 2020). However, the 7.3% prevalence of G. lamblia in this study was lower than the 15.4% found in Nepal (Gupta et al., 2020) and the 27.1% in southern Ethiopia (Hajare et al., 2022). The difference in geography, personal hygiene circumstances of the study subjects, diagnostic techniques utilized, and the application of water, sanitation, and hygiene (WASH) all contribute to the variances in the findings from the previous studies.

In the present study, STST outperformed FECT in terms of detection rates and sensitivity for both G. lamblia and E. histolytica/dispar. Previous investigations (Tello et al., 2012; Gonçalves et al., 2014) made similar conclusion. The rationale might be that STST used a larger stool sample (three gram) than FECT, which only used half a gram of stool. It seems that the likelihood of finding parasites improves when a larger sample size is used. Although it enables detection of motile trophozites, the direct saline wet mount microscopy is poorly sensitive in detecting intestinal protozoa, particularly E. histolytica/dispar and G. lamblia (Obaid, 2022) Therefore, it is crucial in developing nations to use the best available diagnostic techniques, like STST. As an alternate diagnostic approach for intestinal parasitic infections, STST can be employed in this case since it is less expensive, easy to use, and more sensitive to intestinal helminths and protozoa (trophozoite and cysts) than direct saline microscopy and FECT.

In the current study, male and female pupils aged 10 to 14 were, respectively, 1.66 and 1.39 times more likely to be infected with E. histolytica/dispar and G. lamblia than were lower-aged and female participants. A possible explanation could be that pupils become more independent in using the toilet as they get older and participate in more outdoor activities, both of which may raise the risk of E. histolytica/dispar and G. lamblia infections. Additionally, compared to females, males participate in more outdoor activities, including irrigating, caring for pets, and playing games, which could raises their risk of contracting intestinal parasites like E. histolytica/dispar and G. lamblia.

Intestinal parasitic infection is common among people who practice open defecation and in rural areas (Muluneh et al., 2020). In the present study, schoolchildren who lived in rural areas and improperly utilized latrine facilities were 1.97 and 1.49 times more likely to be exposed to E. histolytica/dispar and G. lamblia infections, respectively. These results are in line with those of earlier research findings, where the prevalence was higher among rural participants than urban participants (40.0% vs. 30.0%, p = 0.005) and a higher prevalence of 42.9% was reported among participants who had latrines compared to 29.8% among participants who defecated in the open field (p = 0.04) (Gupta et al., 2020). Similarly, participants who did not have latrine were 2.33 times more likely to be infected than their counterparts, according to a previous study in Ethiopia (Sitotaw et al., 2019). This can be explained by the fact that rural areas have limited knowledge on how to construct and use latrine and that most people there practice open defecation when engaging in outdoor activities.

The spread of intestinal parasitic diseases in children is influenced by poor sanitation and hygiene (Gebru et al., 2023). In the present investigation, E. histolytica/dispar and G. lamblia infections were 2.10 times more likely to occur in schoolchildren who did not wash their hands before meals. This result is in line with research conducted in Jawi town (AOR = 5; 95%CI: 1.34–14.94, p < 0.001) (Sitotaw et al., 2019) and Rama town, Ethiopia (AOR = 2.3; 95%CI: 1.32–4.0, p = 0.001) (Tektook et al., 2019). Similarly, E. histolytica/dispar and G. lamblia infections were 1.48 times more likely to develop in children who played in the soil. These results are consistent with those from an earlier research (Sitotaw et al., 2019). This is understandable given that E. histolytica/dispar and G. lamblia cysts spread through stool and remain contagious up to a few months in soil and water. Playing in the dirt, not washing hands before eating, not washing hands after using the latrine, and drinking contaminated water are all typical problems that increase the risk of contracting E. histolytica/dispar and G. lamblia infections. Consumption of raw vegetables and fruits acts as a potential source for the spread of various parasitic diseases (Alemu et al., 2020). In the current study, children who ate unwashed raw vegetables and fruits were 1.85 times more likely to develop E. histolytica/dispar and G. lamblia infections. This is consistent with previous results (Shahrul Anuar et al., 2012; Sitotaw et al., 2019). The rationale could be that fruits and vegetables are more likely to be contaminated with protozoa cyst stages when they are sold in open markets (on the street) in impoverished nations. Fruits and vegetables typically become contaminated from infected person either directly by stool or indirectly via soil or water. Furthermore, since access to clean water is a major challenge in developing nations, wash water could be a source of contamination for fruits and vegetables. When asked about the source of water for drinking, most participants responded that they used both tap water and surface water (streams, wells, rivers). So, we were unable to analyze the association between intestinal protozoan infection and drinking water sources.

In conclusion, infections with E. histolytica/dispar and G. lamblia were very common in the Amhara region. However, we were unable to distinguish E. histolytica from non-pathogentic species of amoeba, particularly E. dispar and E. moshkovskii. Similarly, we were unable to identify G. lamblia assemblages circulating in humans in the region. We recommend that future molecular studies estimate the actual prevalence of E. histolytica and types of G. lamblia in the study area. Compared to FECT, STST demonstrated a greater detection rate and sensitivity for E. histolytica and G. lamblia infections. Risk factors for E. histolytica/dispar and G. lamblia infections include children between the ages of 10 and 14, living in rural areas, improper latrine use, lack of hand washing before meals and after using the latrine, eating raw vegetables and fruits, and playing in soil. Thus, promoting potable water, proper implementation of WASH, personal hygiene, good latrine use and a one health approach at the community and school levels may aid in reducing E. histolytica/dispar and G. lamblia infections.

CRediT authorship contribution statement

Tadesse Hailu: Writing – review & editing, Writing – original draft, Visualization, Validation, Supervision, Software, Resources, Project administration, Methodology, Investigation, Funding acquisition, Formal analysis, Data curation, Conceptualization. Getaneh Alemu: Writing – review & editing, Software, Methodology, Investigation, Data curation.

Declaration of competing interest

Dear editor, We declare that there have no competing interests.
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