
==== Front
bioRxiv
BIORXIV
bioRxiv
2692-8205
Cold Spring Harbor Laboratory

39091835
10.1101/2024.07.17.603924
preprint
2
Article
An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes
Furlanis Elisabetta
Dai Min
Leyva Garcia Brenda
Vergara Josselyn
Pereira Ana
Pelkey Kenneth
Tran Thien
Gorissen Bram L.
Vlacho Anna
Hairston Ariel
Huang Shuhan
Dwivedi Deepanjali
Du Sarah
Wills Sara
McMahon Justin
Lee Anthony T.
Chang Edward F.
Razzaq Taha
Qazi Ahmed
Vargish Geoffrey
Yuan Xiaoqing
Caccavano Adam
Hunt Steven
Chittajallu Ramesh
McLean Nadiya
Hewit Lauren
Paranzino Emily
Rice Haley
Cummins Alex C.
Plotnikova Anya
Mohanty Arya
Tangen Anne Claire
Shin Jung Hoon
Azadi Reza
Eldridge Mark A.G.
Alvarez Veronica A.
Averbeck Bruno B.
Alyahyay Mansour
Reyes Vallejo Tania
Soheib Mohammed
Vattino Lucas G.
MacGregor Cathryn P.
Banks Emmie
Olah Viktor Janos
Naskar Shovan
Hill Sophie
Liebergall Sophie
Badiani Rohan
Hyde Lili
Xu Qing
Allaway Kathryn C.
Goldberg Ethan M.
Nowakowski Tomasz J.
Lee Soohyun
Takesian Anne E.
Ibrahim Leena A.
Iqbal Asim
McBain Chris J.
Dimidschstein Jordane
Fishell Gord
Wang Yating
06 9 2024
2024.07.17.603924https://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator.
http://biorxiv.org/lookup/doi/10.1101/2024.07.17.603924
nihpp-2024.07.17.603924.pdf
In recent years, we and others have identified a number of enhancers that, when incorporated into rAAV vectors, can restrict the transgene expression to particular neuronal populations. Yet, viral tools to access and manipulate fine neuronal subtypes are still limited. Here, we performed systematic analysis of single cell genomic data to identify enhancer candidates for each of the cortical interneuron subtypes. We established a set of enhancer-AAV tools that are highly specific for distinct cortical interneuron populations and striatal cholinergic neurons. These enhancers, when used in the context of different effectors, can target (fluorescent proteins), observe activity (GCaMP) and manipulate (opto- or chemo-genetics) specific neuronal subtypes. We also validated our enhancer-AAV tools across species. Thus, we provide the field with a powerful set of tools to study neural circuits and functions and to develop precise and targeted therapy.
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