
==== Front
RSC Adv
RSC Adv
RA
RSCACL
RSC Advances
2046-2069
The Royal Society of Chemistry

d4ra05230g
10.1039/d4ra05230g
Chemistry
Trapping of thermally generated ortho- and para-quinone methides by imidazoles and pyrazoles: a simple route to green synthesis of benzopyrone-azole hybrids and their evaluation as α-glucosidase inhibitors†
† Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4ra05230g

https://orcid.org/0009-0007-3673-120X
Myshko Andrii S. ab
https://orcid.org/0000-0002-0370-0630
Mrug Galyna P. a
https://orcid.org/0000-0002-9429-3507
Bondarenko Svitlana P. c
https://orcid.org/0000-0001-9191-9933
Kondratyuk Kostyantyn M. a
https://orcid.org/0000-0003-4370-7041
Kobzar Oleksandr L. a
https://orcid.org/0000-0003-2999-9826
Buldenko Vladyslav M. a
Kozytskiy Andriy V. bd
https://orcid.org/0000-0001-6167-076X
Vovk Andriy I. a
https://orcid.org/0000-0003-3133-601X
Frasinyuk Mykhaylo S. ab
a V. P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine 1 Academician Kukhar Str. Kyiv 02094 Ukraine mykhaylo.frasinyuk@ukr.net

b Enamine Ltd. 78 Winston Churchill Str. Kyiv 02094 Ukraine
c National University of Food Technologies Kyiv 01601 Ukraine
d ChemBioCenter, Taras Shevchenko National University of Kyiv 64 Volodymyrska Str. Kyiv 01601 Ukraine
2 9 2024
29 8 2024
2 9 2024
14 38 2780927815
18 7 2024
27 8 2024
This journal is © The Royal Society of Chemistry
2024
The Royal Society of Chemistry
https://creativecommons.org/licenses/by-nc/3.0/ This article is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.
An efficient green approach for the trapping of in situ generated ortho-and para-quinone methide intermediates by imidazoles and pyrazoles has been developed. A wide range of quinone methide precursors based on simple phenols are compatible with the experimental protocol under mild thermal conditions. This methodology was demonstrated to be suitable for the synthesis of methylene-linked benzopyrone-azole hybrids using naturally occurring coumarin and chromone Mannich bases. In most cases, the products were isolated in good to excellent yields without chromatographic purification. In vitro studies showed that some of the synthesized compounds exhibit inhibitory activity towards α-glucosidase.

We demonstrate a protocol for the synthesis of benzopyrone-azole hybrids in the reaction of pyrazoles and (benz)imidazole with phenolic Mannich bases. The target compounds were studied as inhibitors of α-glucosidase.

National Academy of Sciences of Ukraine 10.13039/501100004742 0124U001926 pubstatusPaginated Article
==== Body
pmcIntroduction

Chemical properties of five-membered nitrogen-containing heterocycles with two or more heteroatoms commonly named “azoles” depend on the number, nature, and positions of heteroatoms in their skeleton. Among them, imidazoles and pyrazoles with isomeric ring systems containing two nitrogen atoms show the most distinct amphoteric properties, which make it possible to afford a wide range of derivatives using various reactions. The importance of these aromatic ring systems is reflected by their presence in naturally occurring histidine, histamine, purines, and several classes of pharmaceuticals. Recent advances in the synthesis and biological activity of compounds based on these heterocyclic cores are discussed in chapters of a monography1 and a book2 as well as references cited herein.

A common and widely used method for the N-alkylation of azoles includes the action of alkyl halides, pseudo halides, or sulfates with azole metal salts or azoles in the presence of a strong base. However, applying these procedures for the synthesis of target compounds bearing phenolic fragments requires the protection of at least the phenolic groups.

On the other hand, the reaction of phenols, azoles, and formaldehyde can be considered as a Mannich reaction whereas azoles play the role of the amino component. Probably due to low nucleophilicity of azoles, the application of only imidazole with simple phenols was reported in the literature,3 and other azoles could be involved in the reaction using 2-naphthol as a very active CH-component.4 However, direct imidazolylmethylation of simple phenols has limitations due to the possible formation of bis-imidazolylmethyl phenols3d or the formation of methylene-bis-phenols.3c

Natural compounds have played a significant role as sources of new drugs over nearly four decades.5 Among them, flavonoids and coumarins are recognized as privileged scaffolds in medicinal chemistry.6 In the framework of benzopyrone-azole hybrids, imidazolylmethylflavones were identified as aromatase inhibitors,7 inhibitors of corticosteroid biosynthesis,8 and antiproliferative agents,9 whereas various 2-azolylmethylchromones were studied as kinase modulators.10 Their synthetic protocols were developed by using the intermediate halomethyl derivatives of benzopyrones and subsequent reaction with azoles. Protection of phenolic groups was required for the obtaining of target hydroxylated derivatives.

Quinone methides with general structures 1 (Fig. 1) are highly polarized and hence reactive under nucleophilic and electrophilic conditions.

Fig. 1 Structural features of ortho- and para-quinone methides.

A few attempts were reported for the synthesis of imidazolylmethylphenols via Michael's addition of imidazole to in situ-generated quinone methides. This route was used for the synthesis of 1H-5-hydroxybenzimidazole,11 6-hydroxyquinoline,12 and 5-hydroxyindole13 derivatives with anti-hepatitis activity, as well as for modification of camptothecin14 for the design of topoisomerase I inhibitors (Fig. 2).

Fig. 2 Biologically active compounds synthesized by Michael's addition of imidazoles/adenine to in situ-generated quinone methides.

Considering the obvious multiple advantages of applying quinone methides in the context of the development of synthetic procedures, we embarked on a study of the feasibility of these intermediates for the conjugation with azaheterocycles in environmentally benign conditions. Herein, we report the realization of this goal; specifically, we describe using dimethyl carbonate for the generation of ortho- and para-quinone methides followed by their trapping by azoles. This protocol proved to be effective for using different quinone methide precursors based on simple phenols and naturally occurring benzopyrones.

Results and discussion

Synthetic and commercial availability of quinone methide precursors could facilitate the development of the conjugation protocols of phenols and azoles. Using them has some lacks and advantages and choosing each should be appreciated in a strategy of the synthesis. As ortho-quinone methide precursors, ortho-dimethylaminomethylphenols are easily accessible using Mannich reaction contrary to para-dimethylaminophenols which can be synthesized by reductive amination often with poor yield. Both precursors such as 2-hydroxymethylphenols and 4-hydroxymethylphenols can be synthesized by the reduction of numerous commercially available substituted hydroxylated aldehydes, but their use for the synthesis of azolylmethylphenols requires high temperatures.15 Moreover, using 2- or 4-methoxymethylphenols requires additional steps for their synthesis, but has an advantage in the case of catechols16 or the synthesis derivatives from natural phenolic compounds bearing methoxymethyl group.17

As a model reaction for the construction of benzopyrone-azole hybrids, we chose a reaction of ortho- and para-substituted phenols bearing dimethylaminomethyl, hydroxymethyl, or methoxymethyl groups 2a–2c and 3a–3c as possible precursors18 for generation of quinone methides with subsequent trapping them with imidazole, pyrazole, and related benzimidazole or 3,5-dimethylpyrazole (as analogs with low nucleophilicity). However, the reported procedure for the capture by azoles of quinone methides in aqueous conditions19 was not applicable for benzopyrone derivatives due to their poor solubility.

In the first stage, to determine the role of solvents and temperature, we investigated the reaction of ortho-substituted phenol 2a–2c with imidazole at various conditions (Table 1). According to these data, dimethyl carbonate (DMC) was chosen as the most acceptable solvent for further development of phenol–azole conjugate synthesis. However, these data were insufficient for elucidation of the most useful leaving group (LG) for the reaction with other azoles, especially in cases of using naturally occurring compound derivatives. Our next experiments were focused on further optimization of the reaction conditions of compounds 2 and 3 with some azoles.

Development and optimization for the synthesis of compound 4aa

	
Compd. (LG)	Solvent	Temp., C	Conversionb, %	Purityc, %	
2a (NMe2)	EtOH	80	n.r.	—	
2a (NMe2)	(MeO)2CO	90	83	85	
2a (NMe2)	1,4-Dioxane	100	53	78	
2a (NMe2)	Toluene	110	50	85	
2a (NMe2)	(EtO)2CO	126	78	82	
2a (NMe2)	DMF	154	72	78	
2b (OH)	EtOH	80	n.r.	—	
2b (OH)	(MeO)2CO	90	43	91	
2b (OH)	1,4-Dioxane	100	39	89	
2b (OH)	Toluene	110	35	90	
2b (OH)	(EtO)2CO	126	38	93	
2b (OH)	DMF	154	53	86	
2c (OMe)	EtOH	80	n.r.	—	
2c (OMe)	(MeO)2CO	90	73	92	
2c (OMe)	1,4-Dioxane	100	45	83	
2c (OMe)	Toluene	110	48	85	
2c (OMe)	(EtO)2CO	126	69	90	
2c (OMe)	DMF	154	67	75	
a A mixture of quinone methide precursors 2a–2c (2 mmol) and imidazole (4 mmol) in an appropriate solvent (5 mL) was refluxed for 24 h.

b Was determinate by LCMS spectra.

c Calculated by LCMS spectra excluding intact reagents.

Phenol-methylene azoles 4 and 5 were synthesized by refluxing compounds 2a–2c and 3a–3c with [benz]imidazole and pyrazoles in dimethyl carbonate for 36 h.

Using 2-dimethylaminomethylphenole 2a was successful for the synthesis of imidazoles 4a, 5a and benzimidazoles 4b, 5b. In the cases of 2- and 4-hydroxybenzylphenols 2b, 3a, and 3c, the target hybrids were synthesized with acceptable to good yield with all azoles. However, the addition of acids such as trifluoroacetic or methane sulfonic was needed to complete the reactions. It should be noted that the reaction of 2-hydroxymethylphenol with imidazole requires high temperature.15a The reactions of imidazole or benzimidazole with 2-methoxymethylphenol 2c and 4-methoxymethylphenol 3b were completed in 24 h whereas conversion of these methoxymethyl derivatives with pyrazole or 3,5-dimethylpyrazole was approximately 40% and was not completed after 72 h.

Thus, imidazole derivatives 4a, 5a, and 5e can be synthesized from all precursors with poor to good yield, whereas applying hydroxymethylphenols 2b, 3a, and 3c was more suitable for the synthesis of benzimidazole derivatives 4b, 5b, and 5f. The more effective conditions for the synthesis of related pyrazole and 3,5-dimethylpyrazole derivatives were achieved using hydroxymethyl phenols 2b, 3a, and 3c in the presence of acids (Table 2).

Scope of substrates in the reaction of phenol derivatives with azolesa

	
a Isolated yield.

b In the presence of 10 mol% of CF3COOH.

Our findings were extended to the application of [benz]imidazole and pyrazoles for the synthesis of azole-phenol hybrids bearing a coumarin or chromone fragment, as attractive compounds which represented naturally occurring bioactive phenols. Thus, umbelliferone (7-hydroxycoumarin, 6a), 4-methylumbelliferone (6b), 2,3-dimethylchromone (6c), soybean isoflavonoids daidzein (7,4′-dihydroxyisoflavone, 6d), formononetin (7-hydroxy-4′-methoxyisoflavone, 6e), cladrin (7-hydroxy-3′,4′-dimethoxyisoflavone, 6f), pseudobaptigenin (7-hydroxy-3′,4-methylenedioxyisoflavone, 6g), 2-methylformononetin (6h), their fluorinated derivatives 2-trifluoroformononetin (6i) and 7-hydroxy-4′-trifluoromethoxyisoflavone (6j) were used for the synthesis of hybrids with the mentioned azoles.

Taking into account that the efficiency for the generation of ortho-quinone methides 7a–7j with fused pyrone ring from complex compounds can be significantly different from simple phenol derivatives 2 or 3, we carried out the reaction of 8-substituted formononetin derivatives with imidazole. As a result, target formononetin-imidazole hybrid 9e was isolated from reaction imidazole with 8-dimethylaminomethylformononetin 8e, (82% yield, 100% purity), 8-hydroxymetylformononetin20 (78% yield, 95% purity), and 8-methoxymethylformononetin20 (84% yield, 95% purity). According to these data and synthetic availability of 7-hydroxycoumarin or 7-hydroxychromone derivatives for the generation of ortho-quinone methides, we used their 8-dimethylaminomethyl derivatives 8a–8j in reaction with azoles in dimethyl carbonate at reflux. As a result, a series of coumarin- and chromone-azole hybrids were synthesized with good to excellent yield and 95–99% purity at the reflux of Mannich bases 8a–8c and 8e–8j with 2-fold excess of azoles in dimethyl carbonate. However, using of 1,4-dioxane was needed in the case of daidzein Mannich base 8d due to its poor solubility in dimethyl carbonate and the formation of by-pass products (Table 3).

Scope of substrates in the reaction of benzopyrone derivatives with azoles

	
a Reaction was performed in 1.4-dioxane.

The reaction of 7-hydroxycoumarin or formononetin Mannich bases 8a, 8e with indazole led to the formation of both possible isomers, 8-(1-indazolylmethyl)-7-hydroxybenzopyrones 13a, 13b and 8-(2-indazolylmethyl)-7-hydroxybenzopyrones 14a, 14b (Scheme 1) with excess of compounds 14.

Scheme 1 Synthesis of indazole-benzopyrone hybrids 13 and 14.

The structures of synthesized compounds 13 and 14 were elucidated using 1D NOESY, HSQC, and HMBS techniques (Fig. 3).

Fig. 3 Key correlations for determination of structures 13 and 14.

We presume that using asymmetrical azoles should afford mixture of both possible N-alkylated regioisomers in the ratio, which can depend on various factors, mainly steric hindrance of substituents and reaction time. In our opinion, a steric hindrance determines the key role, which was demonstrated by indazole use.

The naturally occurring flavonoids, their derivatives, and related compounds are widely studied as α-glucosidase inhibitors.21 The various synthetic heterocycle-containing coumarins and chromones were identified as promising inhibitors of this enzyme.22 In the case of isoflavone derivatives, a substituent at position 8 can influence their inhibitory potency.23 Among the compounds synthesized in this study, the sixteen benzopyrone-azaheterocycle hybrids inhibited α-glucosidase activity by 42–99% at the concentration of 25 μM. The determined IC50 values were in the range of 5.8 μM to 27.4 μM (Table 4). The 4′-trifluoromethoxy derivatives 10h and 12i bearing 1-benzimidazolylmethyl or 3,5-dimethyl-1-pyrazolylmethyl groups at position 8 of the isoflavonoid skeleton were found to be the most effective inhibitors with IC50 values of 9.5 μM and 5.8 μM, respectively. At the same time, 8 non-substituted 4′-trifluoromethoxy isoflavonoid 6j exhibited much lower inhibitory activity (39% at 25 μM) against this enzyme.

Inhibition of α-glucosidase by synthesized benzopyrone-azaheterocycle hybridsa

Compound	Inhibition at 25 μM (%)	IC50, μM	
9b	53.4	23.6 ± 1.8	
9d	42.0	27.0 ± 0.2	
9i	73.7	20.0 ± 2.0	
10b	69.3	21.1 ± 0.7	
10d	64.6	20.6 ± 2.1	
10f	48.6	25.5 ± 7.9	
10g	79.6	15.9 ± 4.7	
10h	99.1	9.5 ± 1.6	
11d	62.5	16.2 ± 3.4	
11j	84.1	14.3 ± 2.0	
12c	42.8	27.4 ± 1.9	
12d	67.3	20.9 ± 2.5	
12h	66.9	21.2 ± 0.7	
12i	94.0	5.8 ± 0.2	
13b	73.0	17.1 ± 2.8	
14b	50.3	23.9 ± 2.3	
Acarboseb		760.6 ± 120.3	
a IC50 values are shown as average value ± standard deviation.

b Reference compound.

Conclusions

In summary, we have demonstrated that ortho- and para-quinone methides can be efficiently generated under thermal conditions in dimethyl carbonate. In contrast to most known methodologies, this protocol allows using water-insoluble compounds and does not require the use of high temperatures and harmful solvents. Various precursors for the generation of quinone methides showed high efficiency in trapping these intermediates by azoles. Also, coumarin and chromone Mannich bases were used for the synthesis of benzopyrone-azole hybrids in good to excellent yield which makes our protocol well-suited for large-scale syntheses. Our protocol could also be implemented for the generation and trapping of ortho-quinone methide intermediates from various naturally occurring 2-hydroxymethyl- or 2-methoxymethylphenols. Several of the synthesized methylene-linked benzopyrone-azaheterocycle hybrids were found to be α-glucosidase inhibitors with IC50 values of 5.8–27.4 μM.

Experimental

Chemistry

1H, 13C, and 19F NMR spectra were recorded on Bruker AVANCE DRX 500 (500/125/470 MHz) or AVANCE III 400 (400/100/376 MHz) spectrometers in CDCl3 [residual CHCl3 (δH = 7.26 ppm) or CDCl3 (δC = 77.16 ppm) as internal standard] or DMSO-d6 [residual SO(CD3)(CD2H) (δH = 2.50 ppm) or SO(CD3)2 (δC = 39.52 ppm) as internal standard]. 2D NMR spectra were recorded on Agilent ProPulse 600 MHz. Melting points were determined in open capillary tubes using the Buchi B-535 apparatus and were uncorrected. IR spectra were recorded on a Bruker Vertex 70. Mass spectra were obtained using an Agilent 1100 spectrometer using APCI (atmospheric-pressure chemical ionization). Elemental analysis was performed on a vario MICRO cube automated CHNS-analyzer. Column chromatography was performed using Macherey-Nagel Silica 60, 0.04–0.063 mm silica gel.

Synthesis of Mannich bases 8a,248b,258e–8i (ref. 26) was described previously. Inhibition of α-glucosidase by compounds 4–5 and 9–14, characteristics of synthesized compounds, and copies of their NMR spectra are provided in ESI†.

General procedure for the synthesis of compounds 4 and 5

A mixture of appropriate compounds 2a–2c, 3a–3c (1 mmol), azole 1a–1d (2 mmol) in the presence of 0.025 mL of trifluoroacetic or methanesulfonic acid (in case of compounds 2b, 3a, or 3c) in 5 mL of dimethyl carbonate was refluxed for 36 h. The reaction mixture was cooled, evaporated, diluted with 20 mL of water, and filtered off. The solid was purified by recrystallization from the ethanol–water mixture.

7-Hydroxy-3-[4-(trifluoromethoxy)phenyl]-4H-chromen-4-one (7j) was synthesized according to typical procedure.27

General procedure for the synthesis of Mannich bases 8c, 8d, and 8i

To a stirred suspension of isoflavonoids 6c, 6d, or 6j (2 mmol) in 10 mL of isopropanol was added 0.3 mL (2.2 mmol, 1.1 eq) of bis(N,N-dimethylamino)methane at 70 °C. The mixture was heated at 80 °C for 2 h and cooled to afford a precipitate that was collected by gravity filtration. The Mannich bases 8 were re-crystallized from isopropanol-hexane.

General procedure for the synthesis of benzopyrone-azole hybrids 9–12

A mixture of benzopyrone Mannich base 8a–8j (1 mmol) and azole 1a–1d (2 mmol) in 5 mL of dimethyl carbonate (or 1,4-dioxane in case of compound 8d) was refluxed for 36 h. The reaction mixture was cooled, diluted with 10 mL of ethanol, and filtered off. The solid was purified by recrystallization from ethanol.

Synthesis of indazole derivatives 13 and 14 was carried out similarly to the general procedure. The mixture of isomeric indazole derivatives was separated by column chromatography using 100 : 1 CH2Cl2–MeOH (for compounds 13a and 14a) or ethyl acetate (for compounds 13b and 14b).

Biology

α-Glucosidase from Saccharomyces cerevisiae and p-nitrophenyl α-d-glucoside as a substrate were purchased from Sigma-Aldrich.

In vitro α-glucosidase inhibition study

The reaction volume (2.0 mL) consisted of 57 mM sodium phosphate buffer (pH 6.8), 1% dimethyl sulfoxide (DMSO), 0.85 mM substrate, inhibitor, and enzyme. Firstly, the reaction mixture, which included buffer, DMSO, inhibitor, and enzyme, was incubated at 37 °C for 5 min. Then, the enzymatic reaction was started by the addition of p-nitrophenyl α-d-glucopyranoside. The activity of α-glucosidase was monitored spectrophotometrically at 400 nm. The inhibition percentage was determined using the equation:% inhibition = 100 − ((Asample × 100)/Acontrol)

The IC50 values were determined from dose-dependent curves using semi-logarithmic plots (percentage of remaining α-glucosidase activity versus logarithm of inhibitor concentration) and linear trend equations. The dose-dependent curve for each of the compounds was obtained from the results of 2–3 experiments, which included the determination of the remaining activity of α-glucosidase at several inhibitor concentrations.

Data availability

The data supporting this article have been included as part of the ESI.†

Author contributions

Conceptualization, A. I. Vovk, M. S. Frasinyuk, O. L. Kobzar; synthesis of compounds, A. S. Myshko, G. P., Mrug, S. P. Bondarenko, K. M. Kondratyuk; elucidation of structures, A. Kozytskiy; biological assays, V. M. Buldenko, O. L. Kobzar; writing – review and editing, M. S. Frasinyuk, O. L. Kobzar, A. I. Vovk.

Conflicts of interest

There are no conflicts to declare.

Supplementary Material

RA-014-D4RA05230G-s001

This work was supported by the National Academy of Science of Ukraine (project 0124U001926).
==== Refs
Notes and references

(a) Yet L. , in Comprehensive Heterocyclic Chemistry IV, ed. D. S. Black, J. Cossy and C. V. Stevens, Elsevier, Oxford, 2022, pp. 1–112
(b) Varvounis G. , Gkalpinos V. , Theodorakopoulou P. and Tsemperlidou E. , in Comprehensive Heterocyclic Chemistry IV, ed. D. S. Black, J. Cossy and C. V. Stevens, Elsevier, Oxford, 2022, pp. 113–307
Agarwal S. , Imidazole-Based Drug Discovery, Elsevier, 2022
(a) Yu S. Deberardinis A. M. Turlington M. Pu L. J. Org. Chem. 2011 76 2814 2819 21405012
(b) de Koning M. C. Horn G. Worek F. van Grol M. Eur. J. Med. Chem. 2018 157 151 160 30096649
(c) Yan J.-M. Zhang Z.-J. Yuan D.-Q. Xie R.-G. Zhao H.-M. Synth. Commun. 1994 24 47 52
(d) Yang L. Luo L. Zhang S. Su X. Lan J. Chen C.-T. You J. Chem. Commun. 2010 46 3938 3940
(a) Acharya P. Venkata Ramana M. M. Korgavkar N. Pavale G. Upadhyay M. Lett. Drug Des. Discovery 2023 20 724 737
(b) Jone Pradeepa S. Tamilvendan D. Susai Boobalan M. Sundaraganesan N. J. Mol. Struct. 2016 1112 33 44
Newman D. J. Cragg G. M. J. Nat. Prod. 2020 83 770 803 32162523
(a) Zhuang C. Zhang W. Sheng C. Zhang W. Xing C. Miao Z. Chem. Rev. 2017 117 7762 7810 28488435
(b) Reis J. Gaspar A. Milhazes N. Borges F. J. Med. Chem. 2017 60 7941 7957 28537720
(c) Raffa D. Maggio B. Raimondi M. V. Plescia F. Daidone G. Eur. J. Med. Chem. 2017 142 213 228 28793973
(d) Costa M. Dias T. A. Brito A. Proença F. Eur. J. Med. Chem. 2016 123 487 507 27494166
(e) Pratap R. Ram V. J. Chem. Rev. 2014 114 10476 10526 25303539
(a) Cavalli A. Bisi A. Bertucci C. Rosini C. Paluszcak A. Gobbi S. Giorgio E. Rampa A. Belluti F. Piazzi L. Valenti P. Hartmann R. W. Recanatini M. J. Med. Chem. 2005 48 7282 7289 16279787
(b) Gobbi S. Cavalli A. Rampa A. Belluti F. Piazzi L. Paluszcak A. Hartmann R. W. Recanatini M. Bisi A. J. Med. Chem. 2006 49 4777 4780 16854084
(c) Leonetti F. Favia A. Rao A. Aliano R. Paluszcak A. Hartmann R. W. Carotti A. J. Med. Chem. 2004 47 6792 6803 15615528
(d) Gobbi S. Martini S. Rozza R. Spinello A. Caciolla J. Rampa A. Belluti F. Zaffaroni N. Magistrato A. Bisi A. Molecules 2023 28 3047 37049810
(e) Recanatini M. Bisi A. Cavalli A. Belluti F. Gobbi S. Rampa A. Valenti P. Palzer M. Palusczak A. Hartmann R. W. J. Med. Chem. 2001 44 672 680 11262078
Gobbi S. Hu Q. Zimmer C. Engel M. Belluti F. Rampa A. Hartmann R. W. Bisi A. J. Med. Chem. 2016 59 2468 2477 26938274
(a) Wang X.-B. Liu W. Yang L. Guo Q.-L. Kong L.-Y. Med. Chem. Res. 2012 21 1833 1849
(b) Potey L. C. Sabale P. M. Sabale V. P. Lett. Drug Des. Discovery 2023 20 1610 1620
(a) Muthuppalaniappan M. , Viswanadha S. , Babu G. and Vakkalanka S. K. V. S. , US Pat., 20110118257, 2011
(b) Nagarathnam D. , Vakkalanka S. K. V. S. , Muthuppalaniappan M. , Viswanadha S. , Babu G. and Bhavar P. K. , WO Pat., 2012151525, 2012
Chen D. Zhai X. Yuan Q. H. Luo J. Xie S. C. Gong P. Chin. Chem. Lett. 2010 21 1326 1329
(a) Liu Y. Feng G. Ma Z. Xu C. Guo Z. Gong P. Xu L. Arch. Pharm. 2015 348 776 785
(b) Zhao C. Zhao Y. Chai H. Gong P. Bioorg. Med. Chem. 2006 14 2552 2558 16326106
Chai H. Zhao Y. Zhao C. Gong P. Bioorg. Med. Chem. 2006 14 911 917 16183290
Naumczuk B. Bocian W. Sitkowski J. Kawęcki R. Kozerski L. New J. Chem. 2019 43 18975 18978
(a) Ma D. Yin Y. Chen Y.-L. Yan Y.-T. Wu J. RSC Adv. 2021 11 15380 15386 35424046
(b) Sidorina N. E. Osyanin V. A. Chem. Heterocycl. Compd. 2007 43 1065 1071
Poddel'sky A. I. Arsenyev M. V. Astaf'eva T. V. Chesnokov S. A. Fukin G. K. Abakumov G. A. J. Organomet. Chem. 2017 835 17 24
(a) Feng Y. Blunt J. W. Cole A. L. J. Munro M. H. G. J. Nat. Prod. 2002 65 1681 1682 12444701
(b) Liu Y.-P. Li Y. Cai X.-H. Li X.-Y. Kong L.-M. Cheng G.-G. Luo X.-D. J. Nat. Prod. 2012 75 220 224 22260257
(c) Mhiri R. Koubaa I. Chawech R. Auberon F. Allouche N. Michel T. Chem. Biodiversity 2020 17 e2000758
Sugimoto H. Nakamura S. Ohwada T. Adv. Synth. Catal. 2007 349 669 679
González-Pelayo S. López L. A. Eur. J. Org Chem. 2017 2017 6003 6007
Frasinyuk M. S. Mrug G. P. Bondarenko S. P. Sviripa V. M. Zhang W. Cai X. Fiandalo M. Mohler J. L. Liu C. Watt D. Org. Biomol. Chem. 2015 13 11292 11301 26416505
Sohretoglu D. Sari S. Phytochem. Rev. 2020 19 1081 1092
(a) Soengas R. G. Silva V. L. M. Ide D. Kato A. Cardoso S. M. Almeida Paz F. A. Silva A. M. S. Tetrahedron 2016 72 3198 3203
(b) Ichale R. Kanhed A. M. Vora A. Mol. Diversity 2024 28 1239 1247
(c) Asgari M. S. Mohammadi-Khanaposhtani M. Kiani M. Ranjbar P. R. Zabihi E. Pourbagher R. Rahimi R. Faramarzi M. A. Biglar M. Larijani B. Mahdavi M. Hamedifar H. Hajimiri M. H. Bioorg. Chem. 2019 92 103206 31445191
Sun H. Li Y. Zhang X. Lei Y. Ding W. Zhao X. Wang H. Song X. Yao Q. Zhang Y. Ma Y. Wang R. Zhu T. Yu P. Bioorg. Med. Chem. Lett. 2015 25 4567 4571 26351039
Mrug G. P. Myshko N. V. Bondarenko S. P. Sviripa V. M. Frasinyuk M. S. J. Org. Chem. 2019 84 7138 7147 31090415
Da Re P. Bonola G. Verlicchi L. J. Med. Chem. 1964 7 162 166 14187369
Frasinyuk M. S. Mrug G. P. Bondarenko S. P. Khilya V. P. Sviripa V. M. Syrotchuk O. A. Zhang W. Cai X. Fiandalo M. V. Mohler J. L. Liu C. Watt D. S. ChemMedChem 2016 11 600 611 26889756
Frasinyuk M. S. Bondarenko S. P. Khilya V. P. Liu C. Watt D. S. Sviripa V. M. Org. Biomol. Chem. 2015 13 1053 1067 25412895
