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BMJ Open Ophthalmol
BMJ Open Ophthalmol
bmjophth
bmjophth
BMJ Open Ophthalmology
2397-3269
BMJ Group BMA House, Tavistock Square, London, WC1H 9JR

37282706
bmjophth-2022-EEBA.16
10.1136/bmjophth-2022-EEBA.16
Oral abstracts
Theme 3 – Corneal storage and microbiological safety measures before/after transplant
1506
16 Reliability and efficiency of corneal thickness measurements using sterile donor tomography in the eye bank
Hamon Loïc 1
Quintin Adrien 1
Mäurer Stephanie 2
Weinstein Isabel 1
Langenbucher Achim 2
Seitz Berthold 1
Daas Loay 1
1 Department of Ophthalmology, Saarland University Medical Center (UKS), Homburg/Saar, Germany
2 Institute of Experimental Ophthalmology, Saarland University, Homburg/Saar, Germany
2022
16 11 2022
7 Suppl 2 Abstracts of the European Eye Bank Association Virtual Meeting, 3–5 March 2022 A7A7
© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
2022
https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ .

Purpose

To evaluate the reliability and efficiency of sterile corneal thickness measurements of donor corneas stored in a plastic culture flask filled with organ culture medium I (MI) or II (MII) based on tomographic data using two different software: the built-in software of the anterior segment OCT (AS-OCT) and a MATLAB self-programmed software.

Methods

Twenty-five (25) donor corneas (50%) stored in MI and 25 (50%) in MII were imaged 5 times consecutively using an AS-OCT. The central corneal thickness (CCT) was measured both with the manual measurement tool of the AS-OCT (=CCTm) and with a MATLAB self-programmed software allowing (semi-)automated analysis (=CCTa). We analyzed the reliability of CCTm and CCTa using Cronbach´s alpha (α) and Wilcoxon signed-rank test.

Results

Concerning CCTm, 68 measurements (54.4%) in MI and 46 (36.8%) in MII presented distortions in the imaged 3D-volumes and were discarded. Concerning CCTa, 5 (4%) in MI and 1 (0.8%) in MII were not analyzable. The mean (± SD) CCTm was 1129 ± 6.8 in MI and 820 ± 5.1 µm in MII. The mean CCTa was 1149 ± 2.7 and 811 ± 2.4 µm, respectively. Both methods showed a high reliability with a Cronbach´s α for CCTm of 1.0 (MI/MII) and for CCTa of 0.99 (MI) and 1.0 (MII). Nevertheless, the mean SD of the 5 measurements was significantly higher for CCTm compared to CCTa in MI (p = 0.03), but not in MII (p = 0.92).

Conclusions

Sterile donor tomography proves to be highly reliable for assessment of CCT with both methods. However, due to frequent distortions regarding the manual method, the (semi-)automated method seems to be more efficient and should be preferred.

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