
==== Front
BMJ Open Ophthalmol
BMJ Open Ophthalmol
bmjophth
bmjophth
BMJ Open Ophthalmology
2397-3269
BMJ Group BMA House, Tavistock Square, London, WC1H 9JR

37282678
bmjophth-2022-EEBA.19
10.1136/bmjophth-2022-EEBA.19
Oral abstracts
Theme 3 – Corneal storage and microbiological safety measures before/after transplant
1506
19 Killing efficacy of a new hypothermic corneal storage medium Kerasave at 4°C against nine micro- organisms frequently found in donor corneas
MSc Laura Giurgola
Tothova Jana D’amato
MSc Claudio Gatto
Rossi Orietta
AL.CHI.MI.A.S.R.L., Ponte San Nicolò, Italy
2022
16 11 2022
7 Suppl 2 Abstracts of the European Eye Bank Association Virtual Meeting, 3–5 March 2022 A8A8
© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
2022
https://creativecommons.org/licenses/by-nc/4.0/ This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ .

Purpose

The aim of the present study was to determine the killing efficacy of Kerasave (AL.CHI.MI.A Srl), a corneal cold storage medium provided with antimycotic tablet against nine contaminants associated corneal infections.

Methods

The killing efficacy of Kerasave was determined after 0, 3 and 14 days of incubation at 4°C in Kerasave after inocumation of the medium with 10(5)–10(6) (CFU) of Candida albicans (CA), Fusarium solani (FS), Aspergillus brasiliensis (AB), Staphylococcus aureus (SA), Enterococcus faecalis (EF), Bacillus subtilis spizizenii (BS), Pseudomonas aeruginosa (PA), Enterobacter cloacae (EC) and Klebsiella pneumoniae (KP). Log10 reductions at different time intervals were determined by the serial dilution plating technique.

Results

After 3 days, Kerasave induced the highest log10

decrease in the concentrations of KP, PA, CA and EC. The 2 log10 decrease was observed for SA and EF. The lowest log10 decrease was observed in BS, AB and FS concentrations. After 14 days, the microbial count of CA, FS, SA, EF, PA and EC further decreased

Conclusions

Corneal cold storage medium Kerasave effectively reduced the microbial concentration of almost all tested mciroorganisms after 3 days and represents a valuable tool to control the microbial contamination of human donor corneas intended for trasnplantation.

special-featureunlocked
==== Body
pmc
