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Yale J Biol MedYale J Biol MedThe Yale Journal of Biology and Medicine0044-00861551-4056Yale Journal of Biology and Medicine Original ContributionEffect of Sesame Oil on Diuretics or ß-blockers in the Modulation of Blood Pressure, Anthropometry, Lipid Profile, and Redox Status Sankar D. a*Rao M. Ramakrishna bSambandam G. cPugalendi K.V. d
a Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Mission’s Research Foundation University, Paiyanoor-603104, Chennai, Tamilnadu, India
b Faculty of Medicine, Rajah Muthiah Medical College and Hospital, Annamalai University, Annamalainagar, Tamilnadu, India
c Prof. Maniarasan Memorial Poly Clinic, Chidambaram, Tamilnadu, India
d Department of Biochemistry, Annamalai University, Annamalainagar, Tamilnadu, India* To whom all correspondence should be addressed: Dr. D. Sankar, Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Mission’s Research Foundation University, Paiyanoor-603104, Chennai, Tamilnadu, India. Tel: 91-9940254517; E-mail: devsankara@yahoo.co.in9 2007 3 2006 79 1 19 26 Copyright ©2006, Yale Journal of Biology and Medicine2006Yale Journal of Biology and MedicineThis is an open access article distributed under the terms of the Creative Commons CC BY-NC license, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use the material for commercial purposes.The study was undertaken to investigate the effect of sesame oil in hypertensive patients who were on antihypertensive therapy either with diuretics (hydrochlorothiazide) or ß-blockers (atenolol). Thirty-two male and 18 female patients aged 35 to 60 years old were supplied sesame oil (Idhayam gingelly oil) and instructed to use it as the only edible oil for 45 days. Blood pressure, anthropometry, lipid profile, lipid peroxidation, and enzymic and non-enzymic antioxidants were measured at baseline and after 45 days of sesame oil substitution. Substitution of sesame oil brought down systolic and diastolic blood pressure to normal. The same patients were asked to withdraw sesame oil consumption for another 45 days, and the measurements were repeated at the end of withdrawal period. Withdrawal of sesame oil substitution brought back the initial blood pressure values. A significant reduction was noted in body weight and body mass index (BMI) upon sesame oil substitution. No significant alterations were observed in lipid profile except triglycerides. Plasma levels of sodium reduced while potassium elevated upon the substitution of sesame oil. Lipid peroxidation (thiobarbituric acid reactive substances [TBARS]) decreased while the activities of superoxide dismutase (SOD), catalase (CAT), and the levels of vitamin C, vitamin E, ß-carotene, and reduced glutathione (GSH) were increased. The results suggested that sesame oil as edible oil lowered blood pressure, decreased lipid peroxidation, and increased antioxidant status in hypertensive patients.
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Introduction
Recently, much attention has been focused on the antioxidant defense system in oxidative stress and cardiovascular diseases. Natural antioxidants and polyunsaturated fatty acids contained in dietary sources are candidates for the prevention of oxidative damage and cardiovascular diseases [1]. Polyunsaturated fatty acids are essential for normal growth and development and may play an important role in the prevention and treatment of coronary heart disease, hypertension, diabetes, and arthritis and other inflammatory and autoimmune disorders. Clinical and epidemiological studies have shown the cardiovascular protective effects of oils rich in polyunsaturated fatty acids (PUFA) [2,3]. In particular, these substances have been reported to lower blood pressure and prevent the development of hypertension [4,5].

Sesame seeds and oil have long been categorized as traditional health food in India and other East Asian countries. Sesame oil has been found to contain considerable amounts of the sesame lignans: sesamin, episesamin, and sesamolin. Sesame oil also contains vitamin E (40 mg/100 g oil), 43 percent of polyunsaturated fatty acids, and 40 percent monounsaturated fatty acids. The lignans present in sesame oil are thought to be responsible for many of its unique chemical and physiological properties, including its antioxidant and antihypertensive properties [6–9]. In the present study, we evaluated the effect of sesame oil (rich in antioxidant lignans, vitamin E, and unsaturated fatty acids) in hypertensive patients on medication with either hydrochlorothiazide or atenolol as antihypertensive therapy.

Materials and Methods
Subjects
The present study consists of patients of both sexes in the age group 35 to 60 years with mild to moderate hypertension, medicated with diuretics (hydrochlorothiazide) or β-blockers (atenolol), who were recruited from the Department of Medicine at Rajah Muthiah Medical College and Hospital, Annamalai University, and Prof. Maniarasan Memorial Polyclinic, Chidambaram, Tamilnadu, India. The criterion for hypertension was systolic blood pressure greater than or equal to 140 mm Hg and diastolic blood pressure greater than or equal to 90 mm Hg, recorded on at least three different occasions after they had rested for 10 minutes supine. Patients with secondary hypertension, hypertension associated with diabetes mellitus, chronic alcoholism, female patients on oral contraceptives, pregnant females, and lactating mothers were excluded from the study. All the subjects gave informed consent to undergo the investigations, and the Ethical Committee of Rajah Muthiah Medical College, Annamalai University, Tamilnadu, India, approved the study.

Study design
A detailed clinical history and physical examination were performed at baseline, and the following measurements were taken: blood pressure; anthropometric measurements, such as height, weight, and body mass index (BMI); lipid profile (total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and triglycerides (TG)); electrolytes (Na⁺, K⁺); lipid peroxidation (TBARS); and enzymic and non-enzymic antioxidants in blood. The patients were advised to continue their antihypertensive drugs as usual. The patients were on medication with hydrochlorothiazide or atenolol for one year prior to the enrollment in the study. The patients were supplied 4 to 5 kg of sesame oil (Idhayam gingelly oil) for a four-member family per month, which constitutes approximately 35 g of oil/day/person. The patients were asked to use sesame oil as the only edible oil for 45 days. At the end of the 45th day, the investigations were repeated. Finally, the patients were asked to switch over to whatever original oil they had been taking before the enrollment of the study for another 45 days. Mostly they were using either sesame oil, groundnut oil, or palm oil interchangeably. All the measurements were repeated at the end of the 90th day of our experiment. The patients were told to strictly adhere to the study protocol. Those who could not follow the protocol until the end of the experiment for any reason were excluded. To avoid much difference in dietary patterns and caloric changes, the same patients have been subjected to substitution of sesame oil and withdrawal of sesame oil substitution.

Anthropometric and blood pressure measurements
Body weight was measured, using a level balance, to the nearest 0.1 kg. Body height was measured without footwear to the nearest 0.5 cm. BMI was calculated as weight (in kg)/height (in m2). Blood pressure was measured by using standard mercury sphygmomanometer.

Biochemical analysis
Fasting blood samples were collected on entering the study (0 days), at the end of 45 days, and after 90 days (i.e., after substitution and withdrawal). Lipid profile, electrolytes, lipid peroxidation, and enzymatic and non-enzymic antioxidants were estimated at the three experimental periods: baseline, after sesame oil substitution, and after withdrawal of sesame oil. All the biochemical determinations were carried out in the Biochemistry Laboratories at Department of Biochemistry, Faculty of Science or Rajah Muthiah Medical College and Hospital, Annamalai University. TC [10], HDL-C [11], and TG [12] concentrations in plasma were determined by standard enzymic methods with a semiautoanalyser (Bayer RA 150, Germany) using commercially available kits (Biocon, Germany). LDL-C was calculated using Friedwald equation [13]. Sodium and potassium [14], TBARS [15], enzymic antioxidants such as superoxide dismutase (SOD) [16], catalase (CAT) [17], glutathione peroxidase (GPx) [18], and non-enzymic antioxidants such as vitamin C [19], vitamin E [20], β-carotene [21], and reduced glutathione (GSH) [22] also were estimated.

Statistics
Student’s t test was applied for comparison between two related samples; values for continuous variables are expressed as means ± SD.

Results
Table 1 shows blood pressure and anthropometric measurements at baseline, sesame oil substitution, and withdrawal of sesame oil. Replacement of sesame oil as cooking oil in hypertensive patients brought their systolic and diastolic blood pressure to normal in a statistically significant fashion. Significant reduction in body weight and body mass index also was noted. After the withdrawal of sesame oil substitution, the values rose again.

Table 2 shows the plasma lipid profile at baseline, after sesame oil substitution, and after withdrawal of sesame oil. No significant alterations were seen in TC, HDL-C, LDL-C, and the TC/HDL-C ratio. TG levels decreased significantly and then rose, following sesame oil substitution and withdrawal, respectively.

Table 3 shows the plasma levels of electrolytes at baseline, after sesame oil substitution, and after withdrawal of sesame oil. Plasma sodium levels decreased significantly and then rose, following sesame oil substitution and withdrawal, respectively. Potassium levels increased significantly upon sesame oil substitution and subsequently decreased, but within normal limits.

Table 4 shows the levels of TBARS, enzymic and non-enzymic antioxidants at baseline, after sesame oil substitution, and after withdrawal of sesame oil. Significant reduction in TBARS was noted, and the values were almost maintained even after withdrawal of sesame oil. Plasma CAT and erythrocyte membrane bound SOD activities significantly increased, while erythrocyte membrane bound GPx activity decreased gradually from sesame oil substitution to withdrawal. Significant elevations of vitamin C, vitamin E, ß-carotene, and reduced glutathione were observed, and the levels decreased once sesame oil substitution was stopped.

Discussion
In the present study, substitution of sesame oil lowered systolic and diastolic blood pressure remarkably in hypertensive patients. Studies reported that sesamin, a lignan from sesame oil, exerts antihypertensive action by interfering with renin-angiotensin system, as the lignan is more effective on the renin-independent DOCA (Deoxycorticosterone acetate) -salt hypertension than on the renin-independent 2K (two kidney), 1C (one clip) renal hypertensive model [6,8]. In another study using the rat aortic ring, sesamin produced Ca²⁺ antagonistic vasodilatory activity [8]. This pharmacological action, at least in part, may contribute to its antihypertensive activity. Natural antioxidants and polyunsaturated fatty acids show protective function against hypertension [1]. Supplementation of vitamin E reduced blood pressure in mild hypertensive patients and was associated with a remarkable decrease in systolic and diastolic blood pressure [23]. The fatty acid composition of dietary fat is a key determinant of membrane fatty acid composition [24]. As PUFA substitution increases the fluidity of the bilipid layers, the distensibility of biomembranes may increase. The blood pressure-lowering effect of sesame oil may be due to its richness of antioxidant lignans (sesamin, episesamin, sesamol, and sesamolin), vitamin E, and unsaturated fatty acids.

The risk of hypertension increases progressively with higher levels of body weight or BMI and parallels the degree of obesity. The association between BMI and blood pressure consistently has been shown in numerous studies [25]. Numerous studies consistently have documented that for those who are already overweight, weight loss significantly reduces blood pressure and the incidence of subsequent hypertension. Large, randomized trials of weight reduction in adults with hypertension have shown significant reductions in blood pressure in response to weight loss [26]. Studies suggest that polyunsaturated fatty acid increases the plasma levels of leptin, which, in turn, would facilitate the reductions of weight [27]. Polyunsaturated fatty acids in sesame oil also may play a role in the reduction of body weight in our study, which in turn may reduce the blood pressure. The reduction of body weight and body mass index in our study mainly may be due to sesame oil substitution, since the values increased once the sesame oil substitution was withdrawn.

Prior studies in rats have been shown that sesame lignans (sesamin and/or episesamin) lower serum and liver cholesterol concentrations by inhibiting absorption and synthesis of cholesterol [28]. We did not find a cholesterol-lowering effect in hypertensive patients on medication with diuretics or ß-blockers. This may be due to the negative effect of diuretics and ß-blockers on lipids. Recently, the Scientific Advisory of the American Heart Association reported that high monounsaturated fatty acids diets tend to lower triglyceride concentrations [29]. We found that substitution of sesame oil as edible oil lowered plasma triglyceride concentrations.

Reports suggested that antihypertensive compounds modulate the Na⁺-K⁺ pump and thereby maintain the electrolytes levels in hypertensive patients. Cardiac output is influenced by blood volume, which is greatly dependent on body sodium. Thus, sodium excretion is central to blood pressure modulation. Decreasing sodium excretion increases fluid volume and leads to high cardiac output. Potassium can influence cell membrane stabilization and vascular smooth muscle relaxation [1]. In our present study, we found that plasma levels of sodium decreased while potassium levels increased upon the substitution of sesame oil. However, the mechanism of reduction of sodium and elevation of potassium upon sesame oil substitution is not known.

Thiobarbituric acid reactive substances, a measure of lipid peroxidation, decreased significantly upon sesame oil substitution. It has been reported that sesamolin, a lignan present in sesame oil, reduced lipid peroxidation in rats [30]. Sesamin and sesamolin may potentiate the effect of vitamin E and they themselves act as antioxidants, which, in turn, may reduce lipid peroxidation. In our study, plasma levels of TBARS did not change even after withdrawal of sesame oil substitution. Perhaps the lignans stored in the body may be responsible for this.

The role of the antioxidant defense system, which includes superoxide dismutase (EC 1.15.1.1; Cu/Zn SOD), catalase (EC 1.11.1.6; CAT), and glutathione peroxidase (EC 1.11.1.9; GSH-Px), in protection against oxidative insults is well characterized, and it has been suggested that this antioxidant defense system may be influenced by nutrition [31]. Enzymatic antioxidants, such as SOD and CAT, play an important role in the conversion of ROS to oxygen and water. SOD is a well-known scavenger enzyme preventing the cell from oxidative stress. CAT is an important antioxidant enzyme whose physiological role is to detoxify H2O2 into oxygen and water and thus limit the deleterious effects of reactive oxygen species. Cells maintain their vital functions against oxidative damage with the help of a system that involves GPx, SOD, CAT, glutathione reductase, some trace elements, and vitamins A and E. The increase of SOD and CAT may be due to decreased utilization, since lipid peroxidation levels are low. GPx probably decreased due to the decreased synthesis, since lipidperoxidation levels were low. Vitamin E has been recognized as one of the body’s major natural antioxidants. Sesame oil contains 40 mg of vitamin E per 100 g of oil [32]. Vitamin E has several potentially cardio-protective effects: It decreases lipid peroxidation and spares glutathione [33,34]. Vitamin E has been shown to lower blood pressure in spontaneously hypertensive rats [35]. In the present study, plasma levels of vitamin E increased upon substitution, which could be due to the greater availability of vitamin E in sesame oil.

Elevation of vitamin C upon the substitution of sesame oil could be due to the decreased utilization or due to increase in the levels of GSH, because vitamin C and GSH are synergistic antioxidants [36]. Epidemiological reports show that carotenoids may play a preventive role in cardiovascular disease [37]. Plasma levels of ß-carotene rose significantly upon the substitution of sesame oil, which could be due to the sparing action of vitamin E and sesame lignans.

In conclusion, substitution of sesame oil, as the sole edible oil, lowered blood pressure in hypertensive patients who were taking diuretics and ß-blockers. Sesame oil also has beneficial effects on the levels of triglyceride, electrolytes, lipid peroxidation, and antioxidants.

We thank V.V.V. & Sons for the cost-free supply of ldhayam gingelly oil to carry out the research work.

Abbreviations
BMIbody mass index

CATcatalase

GPxglutathione peroxidase

GSHglutathione

HDL-Chigh density lipoprotein cholesterol

LDL-Clow density lipoprotein cholesterol

PUFApolyunsaturated fatty acids

SODsuperoxide dismutase

TBARSthio-barbituric acid reactive substances

TCtotal cholesterol

Table 1 Blood pressure and anthropometric measurements at baseline, sesame oil substitution, and withdrawal of sesame oil (values represent means ± SD).
Parameters	Age 35 to 60 (n = 50) 	
Baseline	Sesame oil substitution	Withdrawal of sesame oil	
Systolic blood pressure (mm HG)	144.25 ± 10.50	124.88 ± 8.0a	144.85 ± 10.501	
Diastolic blood pressure (mm Hg)	97.9 ± 7.80	83.80 ± 6.0a	97.60 ± 7.561	
Height (cm)		159.9 ± 5.0		
Weight (kg)	74.30 ± 7.5	68.5 ± 7.0a	70.0 ± 7.0b	
Body mass index	29.40 ± 3.0	27.08 ± 3.30a	27.7 ± 1.90a	
a,1: p < 0.001; b: p < 0.01

a, b: as compared with baseline value

1: as compared with sesame oil substitution

Table 2 Lipid profile at baseline, sesame oil substitution, and withdrawal of sesame oil (values represent means ± SD).
Parameters	Age 35 to 60 (n = 50) 	
Baseline	Sesame oil substitution	Withdrawal of sesame oil	
TC (mg/dl)	220 ± 15.5	217 ± 18.0	223 ± 20.0	
HDL-C (mg/dl)	46.0 ± 2.5	47.0 ± 1.8	46.5 ± 2.4	
LDL-C (mg/dl)	136.0 ± 7.0	138.0 ± 11.0	139.0 ± 16.4	
TG (mg/dl)	194.80 ± 8.5	159 ± 9.0a	179.9 ± 11.50a,1	
TC/HDL ratio	4.8 ± 1.7	4.7 ± 1.0	4.76 ± 1.5	
a,1: p < 0.001

a: as compared with baseline value

1: as compared with sesame oil substitution

Table 3 Electrolytes at baseline, sesame oil substitution, and withdrawal of sesame oil (values represent means ± SD).
Parameters	Age 35 to 60 (n = 50) 	
Baseline	Sesame oil substitution	Withdrawal of sesame oil	
Sodium (mEq/l)	137.5 ± 1.5	130.0 ± 1.0a	136.5 ± 1.81	
Potassium (mEq/l)	4.0 ± 0.18	4.72 ± 1.15a	4.05 ± 0.181	
a,1: p < 0.001

a: as compared with baseline value

1: as compared with sesame oil substitution

Table 4 TBARS, enymic, and non-enzymic antioxidants at baseline, sesame oil substitution, and withdrawal of sesame oil.
Parameters	Age 35 to 60 (n = 50) 	
Baseline	Sesame oil substitution	Withdrawal of sesame oil	
TBARS (nmol/dl)	6.0 ± 1.40	3.40 ± 0.80a	3.20 ± 0.82a	
E SOD (Ux/mg Hb)	3.0 ± 0.46	4.70 ± 0.70a	3.6 ± 0.14b,1	
E GPx (Uy/min mg Hb)	8.80 ± 0.51	7.70 ± 0.12a	5.40 ± 0.15a,1	
P CAT (Uz/mg protein	5.4 ± 0.34	7.21 ± 0.66a	5.67 ± 0.351	
Vitamin C (mg/dl)	0.90 ± 0.09	1.08 ± 0.09a	0.80 ± 0.10c,1	
Vitamin E (mg/dl)	1.60 ± 0.44	2.0 ± 0.20a	1.40 ± 0.20d,1	
ß-carotene (mg/dl)	0.50 ± 0.08	0.70 ± 0.05b	0.50 ± 0.032	
Reduced glutathione (mg/dl)	15.0 ± 3.0	27.5 ± 3.0a	18.5 ± 2.0b,1	
a,1: p < 0.001; b,2: p < 0.01; c,3: p < 0.05; d,4: p < 0.02

a, b,c,d: as compared with baseline value

1,2: as compared with sesame oil substitution

P, Plasma; E, Erythrocyte membrane; xOne unit of activity was taken as the enzyme concentration which gave 50 percent inhibition of NBT reduction in one minute; yµg of glutathione consumed/min/mg Hb; zµmole of H2O2 consumed/min/mg protein.
==== Refs
Das UN   Interaction(s) between nutrients, essential fatty acids, eicosanoids, free radicals, nitric-oxide, antioxidants and endothelium and their relationship to human essential hypertension Med Sci Res 2000 28 75 83 
Morris MC   Dietary fats and blood pressure J Cardiovasc Risk 1994 1 21 30 7614413 
Sacks FM  Hebert P  Appel LJ  Borhani NO  Applegate WB  Cohen JD   The effect of fish oil on blood pressure and high density-lipoprotein cholesterol levels in phase I of the trials of Hypertension Prevention Collaborative Research Group J Hypertens 1994 12 12 Suppl S23 S31 
Mori TA  Bao DQ  Burke V  Puddey IB  Beilin LJ   Docosahexaenoic acid but not eicosapentaenoic acid lowers ambulatory blood pressure and heart rate in humans Hypertension 1999 34 253 260 10454450 
Prisco D  Paniccia R  Bandinelli B  Fillippini M  Francalanci I  Giusti B  Giurlani L   Effect of medium-term supplementation with a moderate dose of n-3 polyunsaturated fatty acids on blood pressure in mild hypertensive patients Thromb Res 1998 91 105 112 9733153 
Matsumura Y  Kita S  Morimoto S  Akimoto K  Furuya M  Oka N  Tanaka T   Antihypertensive effect of sesamin. I. Protection against deoxycorticosterone acetate-salt-induced hypertension and cardiovascular hypertrophy Biol Pharm Bull 1995 18 1016 1019 7581242 
Kita S  Matsumara Y  Morimoto S  Akimoto K  Furuya M  Oka N  Tanaka T   Antihypertensive effect of sesamin. II. Protection against two-kidney, one-clip renal hypertension and cardiovascular hypertrophy Biol Pharm Bull 1995 18 1283 1285 8845824 
Matsumura Y  Kita S  Tanida Y  Taguchi Y  Morimoto S  Akimoto K  Tanaka T   Antihypertensive effect of sesamin. III. Protection against development and maintenance of hypertension in stroke-prone spontaneously hypertensive rats Biol Pharm Bull 1998 21 469 473 9635502 
Yamashita K  Iizuka Y  Imai T  Namiki M   Sesame seed and its lignans produce marked enhancement of vitamin E activity in rats fed a low alpha-tocopherol diet Lipids 1995 30 1019 1028 8569430 
Allain CC  Poon LS  Chan CSG  Richmond W  Fu PC   Enzymatic determination of total serum cholesterol Clin Chem 1974 20 470 4818200 
Warnick GR  Nguyan T  Albers AA   Comparison of improved precipitation methods for quantification of HDL-cholesterol Clin Chem 1985 31 217 222 2578337 
Cole TG  Klotzsch SG  McNamara J   Rifai N  Warnick GR  Dominiczak MH   Measurement of triglyceride concentration Handbook of Lipoprotein Testing 1997 Washington, D.C. AACC Press 155 226 
Friedewald WT  Lewy RI  Fredrickson DS   Estimation of concentration of the low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge Clin Chem 1972 18 499 502 4337382 
Gowenlock A   Electrolyte and acid base disturbances Varley’s Practical Clinical Biochemistry 1988 6th ed Boca Raton CRC Press 550 600 
Nichans WG Jr.  Samuelson B   Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation Eur J Biochem 1968 6 126 130 4387188 
Kakkar P  Das B  Viswanathan PN   A modified spectrophotometric assay of superoxide dismutase Ind J Biochem Bio Phys 1978 21 130 
Sinha KA   Colorimetric assay of catalase Anal Biochem 1972 47 389 394 4556490 
Rotruck JT  Pope A  Ganther HE  Swanson AB   Selenium: biochemical roles as component of glutathione peroxidase Science 1973 179 588 590 4686466 
Roe JH  Kuether CA   Detection of ascorbic acid in whole blood and urine through the 2, 4-dinitro phenyl-hydrazine of dehydro ascorbic acid J Biol Chem 1943 147 399 407 
Baker H  Frank O  De Angelis B  Feingold S   Plasma tocopherol in man at various times after ingesting free on acetylated tocopherol Nutr Res Int 1980 21 399 407 
Bradely DW  Horneback CLC   Clinical evaluation of an improved TFA micro method for plasma and serum vitamin A Biochem Med 1973 7 78 86 4684090 
Ellaman GL   Tissue sulfhydryl groups Arch Biochem Biophys 1959 82 70 77 13650640 
Boshtam M  Rafiei M  Sadeghi K  Sarraf-Zadegan N   Vitamin E can reduce blood pressure in mild hypertensives Int J Vitam Nutr Res 2002 72 309 314 12463106 
Clandinin MT  Cheema S  Field CJ  Garg ML  Venkatramam J  Clandinin TR   Dietary fat: exogenous determination of membrane structure and cell function FASEB J 1991 5 2761 2769 1916101 
Arroya P  Fernandez V  Avila-Rosas H   Overweight and hypertension: data from the 1992-1993 Mexican Survey Hypertension 1997 30 646 649 9322997 
The Expert Panel: Report of the National Cholesterol Education Program Panel on detection, evaluation and treatment of high blood cholesterol in adults Arch Intern Med 1988 148 36 64 3422148 
Hynes GR  Heshka J  Chadee K  Jones PJ   Effect of dietary fat type and energy restriction on adipose tissue fatty acid composition and leptin production in rats J Lipid Res 2003 44 893 901 12562868 
Ogawa H  Sasagawa S  Murakami T  Yoshizumi H   Sesame lignans modulate cholesterol metabolism in the stroke-prone spontaneously hypertensive rat Clin Exp Pharmacol Physiol Suppl 1995 22 S310 S312 9072406 
Kris-Etherton P   Monounsaturated fatty acids and risk of cardiovascular disease Circulation 1999 100 1253 1258 10484550 
Kang M-H  Naito M  Tsujihara N  Osawa T   Sesamolin inhibits lipid peroxidation in rat liver and kidney J Nutr 1998 128 1018 1022 9614163 
Huang CY  Chen LH  Osio Y  Cohen DA   Effects of diet composition on liver antioxidant defense and detoxification enzymes in mice with murine AIDS Nutr Res 1994 14 1841 1851 
Fukuda Y  Nagate M  Osawa T  Namiki M   Chemical aspects of the antioxidative activity of unroasted sesame seed oil and the effect of using the oil for frying Agri Biol Chem 1986 50 857 
Vasdev S  Barret B  Longerich L  Ford CA   Sharma BK  Takeda N  Ganguly NK  Singal PK   Role of aldehydes in hypertension Adaptation Biology and Medicine, Vol. 1 1997 New Delhi Narosa Publishing House 326 339 
Sharma A  Kharb S  Chugh SN  Kakkar R  Singh GP   Evaluation of oxidative stress before and after control of glycemia and after vitamin E supplementation in diabetic patients Metabolism 2000 49 160 162 10690938 
Newaz MA  Nawal NNA   Effect of α-tocopherol on lipid peroxidation and total antioxidant status in spontaneously hypertensive rats Am J Hypertens 1998 11 1480 1485 9880131 
Infers H  Sies H   The production of ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E Eur J Biochem 1988 174 353 3383850 
Gerster H   Potential role of ß-carotene in the prevention of cardiovascular disease Int J Vit Nutr Res 1991 61 277

