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Evid Based Complement Alternat MedEvid Based Complement Alternat MedeCAMEvidence-based Complementary and Alternative MedicineEvidence-based Complementary and Alternative Medicine : eCAM1741-427X1741-4288Oxford University Press 1734223710.1093/ecam/nel066ReviewDoes the Consumption of Green Tea Reduce the Risk of Lung Cancer Among Smokers? Liang Wenbin Binns Colin W. Jian Le Lee Andy H. School of Public Health, Curtin University of Technology Perth, AustraliaFor reprints and all correspondence: Professor Andy H. Lee, School of Public Health, Curtin University of Technology, GPO Box U1987, Perth, WA 6845, Australia. Tel: +61-8-92664180; Fax: +61-8-92662958; E-mail: Andy.Lee@curtin.edu.au3 2007 24 10 2006 4 1 17 22 23 11 2005 25 8 2006 © 2006 The Author(s).2006This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commerical use, distribution, and reproduction in any medium, provided the original work is properly cited.Experimental and epidemiological studies were reviewed to assess whether the consumption of green tea could reduce the risk of lung cancer in smokers. Articles published since 1990 were located by searching electronic databases PubMed, Ovid and Science Direct, using keywords ‘lung cancer’, ‘tea’ and ‘smoking’ without any restriction on language. After relevant articles had been located, further papers were obtained from their reference lists. Evidence from experimental studies (in vitro animal and human trials) suggested that regular intake of green tea may be protective against tobacco carcinogens. However, the mechanism behind the protective effect is only partly understood. In most of the epidemiological studies reviewed, the green tea exposure was within 5 years of the interview or follow-up, which would coincide with the induction period and latent period of lung cancer. Longer term studies are thus needed to further quantify the cancer risk. There is some evidence suggesting regular intake of green tea at high level (>3 cups per day) may reduce the risk of smokers developing lung cancer. Improvement in measuring green tea intake is required in order to confirm the evidence from epidemiological studies.

carcinomapolyphenolssmokingtobacco
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Introduction
Lung cancer has become one of the leading causes of death in both developed and developing countries (1,2). Most of the deaths from lung cancer are due to smoking tobacco products. Worldwide in 2000, tobacco smoking contributed 77% of the lung cancer deaths in males aged 30–69 years, and 82% in males over 70 years (1,2). In 2003, for example, the prevalence of smoking in China was estimated to be 58.9% among adult males, while Chinese smokers are four times more likely to die from lung cancer than non-smokers (3).

Although elimination of tobacco smoke exposure is the obvious strategy to control lung cancer, no country has been completely successful in implementing an effective intervention. The efficacy of smoking cessation programs may be enhanced if they are conducted in conjunction with other complementary therapies. Epidemiological and experimental studies have suggested that green tea may be protective against cancer in different sites (4–21). In particular, recent evidence from in vitro, animal and human trial studies indicated the possibility that the consumption of green tea may reduce the risk of lung cancer among smokers (2,22–35), yet findings from epidemiological studies remained inconsistent.

The aim of this review is to assess evidence from experimental and epidemiological studies whether green tea is a risk modifier for those exposed to tobacco smoke. The focus is on the measurement of green tea exposure undertaken in these studies.

Methods
Articles published since 1990 were located by searching electronic databases PubMed, Ovid and Science Direct, using keywords ‘lung cancer’, ‘tea’ and ‘smoking’ without any restriction on language. After relevant articles had been located, further papers were obtained from their reference lists. A total of 78 relevant articles were eventually found.

Results and Discussion
In vitro and Animal Studies
Both in vitro and animal studies have demonstrated that green tea or tea polyphenols have a chemopreventive effect against carcinogens from tobacco and is dependent on both the period and dose of the exposure.

Period of exposure
The period of exposure to green tea or tea polyphenols and the period of exposure to carcinogens must overlap. In one in vitro study, green tea polyphenols were introduced once into the living cell environment 2 h before exposing the cells to a smoke solution for 30 min. Result showed that green tea polyphenols can reduce DNA strand breakage induced by cigarette smoke in cultured human bronchial cells A549 (32). In another in vitro study, human lymphoid cells were exposed to one single dose of green tea extracting solution 2 h before adding benzo(a)pyrene-diol-epoxide (BPDE)—a metabolic production of benzo(a)pyrene (B(a)P), which is a major component of tobacco. The results demonstrated that green tea can significantly reduce DNA damage caused by BPDE (22). Similarly in four other animal studies (23,29,33,34), mice were given green tea prior to or within 48 h of exposure to tobacco carcinogens, and the green tea was continuously administered for 16 weeks or 9 months. Other experimental conditions such as diet, humidity, temperature and dark/light cycle were controlled. Three out of the four studies found a reduction in the incidence and the size of lung cancer tumors due to drinking green tea (23,33,34).

Dose of exposure
The preventive effect of green tea is likely to be related to the concentration of green tea as well as the dose of carcinogens. In experimental studies both cells and animals were often treated with relatively high doses of carcinogens and green tea. For example, in a study which showed a protective effect of green tea, the concentration of tobacco carcinogens used was equivalent to the amount contained in the sidestream smoke from one cigarette dissolved in 24 ml of water, while the concentration of green tea polyphenol was set at 250 μg ml−1, corresponding to 116.5 μg ml−1 of epigallocatechin-3-gallate (EGCG) (32). Moreover, the bioavailability of different green tea polyphenols has been a concern in animal and human studies. Green tea polyphenols are not usually stable, specifically, in water, EGCG can oxidize more than 50% within 12 h, yet green tea used in experimental studies was typically prepared every 48 h (33,34) to every 7 days (29).

In some studies where mice were exposed to both NNK [4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone] and green tea, the NNK dosage administered was usually equal to that experienced by a smoker in a lifetime (36). In one experiment mice were randomized into different groups and given green tea solution at concentrations of 0.2%, 0.4% and 0.6% as drinking fluid 24 h after treating with NNK. Comparing with the control group that drank only water, the protective effect of green tea was only evident at the 0.6% concentration level (33). In another study (29), mice were exposed to high levels of environmental tobacco smoke for 5 months, while being given 1.25% green tea (2.5 g tea leave mixed with 200 ml boiling water). The results indicated green tea had little effect on reducing tumor incidence or multiplicity of the tumors. However, none of the animals in the green tea treatment group experienced early death while >10% of the mice in the control group experienced early deaths, probably due to an overdose of carcinogens exposure (29). Findings from these experiments suggest that green tea may not be effective to inhibit the formation or growth of lung tumor under high level of carcinogens exposure and over a long duration.

Human Trials
Short-term effect
Because a single dose of green tea intake only produces a short-term antioxidant effect, regular consumption seems necessary. In a human study (37), 21 non-smokers consumed 300 ml of green tea solution or about three cups. Ferric reducing ability of plasma (FRAP-assay) was used to determine the antioxidant activity in plasma. The results of this study showed that green tea consumption produced a significant increase in antioxidant activity within 30–120 min of exposure, but after 120 min the antioxidant activity appeared to decrease. Another human trial with 10 healthy subjects similarly found a short-term increase of plasma antioxidant induced by drinking two to three cups of green tea at one time (38). Such a short-term increase seemed to correspond with the relatively short half-life of tea polyphenols (30). In one pharmacokinetic study (39), 30 healthy participants ingested 200 mg of EGCG equivalent to two cups of green tea. Blood samples were collected at 0.5, 1, 2, 4, 6, 8 and 24 h after the EGCG administration. This experiment found that the mean half-life of EGCG in plasma was only 118 min.

Protective effect from regular consumption
Regular green tea consumption could be protective over an extended period. In one pilot study (35), three non-smokers and three smokers (consumed more than 10 cigarettes per day) drank two cups of green tea in the morning, two cups in the afternoon and one cup at night daily for 4 weeks. Each cup of green tea contained 400–500 mg of green tea extract. Oral keratinocytes were harvested from each subject for analysis. Outcomes included a reduction in smoking-induced DNA damage, increased apoptosis and a further decrease in the number of cells carrying damaged DNA (35). In a randomized control trial (27), 133 smokers who smoked at least 10 cigarettes per day during the past year were randomized into three groups consuming at least four cups daily of either (i) decaffeinated green tea, (ii) decaffeinated black tea, or (iii) water. After 4 months of intervention, smokers who drank decaffeinated green tea had a significant decrease in their urinary 8-hydroxydeoxyguanosine (8-OHdG) level, but no significant changes were observed in both water and black tea groups. Similar reduction in urinary 8-OHdG level was observed among HbsAg positive volunteers who took daily capsules containing green tea polyphenols equivalent to two to four cups of tea for 3 months (40). In an intensive observational study (25), the frequencies of sister-chromatid exchange (SCE) in peripheral lymphocytes were measured as markers of smoking-induced DNA damage. The frequencies of SCE were significantly higher among smokers who were non-tea drinkers, than those of non-smokers and smokers who consumed green tea at least two to three cups per day during the past 6 months. The differences between the latter two groups, however, were not significant. It is possible that consuming high levels of green tea over a long period may reduce the DNA damage caused by tobacco smoking.

Lung tumor treatment
Only one clinical trial used green tea for treatment of lung cancer (41). In this Phase I study, 17 advanced lung cancer patients were given green tea extract at a single dose of 3 g m−2 per day for 4 weeks. The dosage equates to 20 cups of green tea, which has been found to be the maximum tolerated dose. Unfortunately, no effect of the treatment was observed. Therefore, green tea is unlikely to be an effective cytotoxic agent against existing tumor.

Limitation of human trials
Except two studies (27,40), most human intervention studies involved small sample sizes generally less than 50 subjects. In addition to ethical concerns, there were neither direct measurement on the concentration of green tea polyphenols in lung tissue nor on the change of lung tissue after green tea exposure. Instead, the effects of green tea were measured by the change in DNA damage and apoptosis of oral cells (35), changes in biomarkers of DNA damage such as 8-OHdG and SCE (25,27,40), and changes in plasma antioxidant capacity (30,37,38). Trials with sufficient sample size and longer period of follow-up are needed to confirm the protective effect of green tea.

Mechanisms Based on Experimental Studies
As shown in Fig. 1, carcinogens contained in cigarette smoke are activated by Phase I enzymes in the body, leading to the formation of DNA adducts (36). If DNA adducts escape cellular repair mechanisms, it may result in miscoding and eventually a stable mutation. Blocking any step in the pathway would reduce the incidence of lung cancer among smokers (36). Table 1 provides the possible mechanisms of chemoprevention effect of green tea. The evidence suggested that catechins in green tea, especially EGCG, may prevent the formation of a mutated cell.
Figure 1. Mechanism of tobacco carcinogens inducing lung cancer.


Table 1. Possible mechanism of action

Possible mechanism of chemoprevention effect of green tea	References	
Acting as antioxidant to prevent DNA damage and reduce the formation of DNA adducts	(11,22,25,27,35,40)	
Increasing apoptosis and preventing the persistence of miscoding DNA (possibly by inducing gene regulation)	(11,26,31,33,35,40,43,44)	
Activating Phase II detoxifying enzymes, and increasing the excretion of carcinogens (possibly by inducing gene regulation)	(2,8,28,31)	


In both animal and in vitro studies, green tea was found to increase the activities of Phase II enzymes (2,8,28,31). Phase II enzymes transform carcinogens into non-toxic molecules for subsequent excretion (42). The amount of carcinogens that may be further activated, as well as DNA adducts, are thus reduced. The antioxidant properties of green tea have also been demonstrated in trials involving smokers. It reduced the urine concentration of 8-OHdG, a strong biomarker of DNA damage (27). Moreover, green tea extract could inhibit the formation of human DNA adducts (11,22,35).

Once carcinogen DNA adducts have formed, persistent mutation is likely unless cells with DNA adducts are removed by apoptosis (36). Human (35), animal (11,33) and in vitro studies (26,43,44) have shown that green tea can induce apoptosis among injured cells exposed to carcinogens from tobacco smoke. However, smoking may be regarded as a chronic exposure, since long duration of tobacco smoking has greater impact on lung caner risk than the amount smoked per day (45). Therefore, a long exposure to green tea is needed in order to reduce the damage caused by tobacco carcinogens.

Epidemiological Studies
Induction time and latent period of lung carcinoma
Latent period refers to the time between disease occurrence and detection, while tumor induction time commences when the effect of the exposure occurs until all of the component causes involved in one sufficient cause are satisfied—the time of disease occurrence (46). Exposure within the induction and latent periods is irrelevant to the formation of a tumor (47). Moreover, the incidence of lung cancer does not appear to be lower among ex-smokers who quit smoking within 5 years than current smokers (48,49). This indicates that the sum of induction period and latent period of lung cancer caused by tobacco smoking may be longer than 5 years. Logically, no reduction in incidence of lung carcinoma is expected among current smokers who drink green tea for 5 years prior to the calculation of incidence, regardless of their consumption level.

Measurement of green tea exposure and other limitations
Table 2 summarizes epidemiological studies on tea and lung cancer. Three case–control studies (50–52) found a significantly lower risk of lung cancer among non-smokers with a high consumption of green or black tea, but no protective effect was evident among smokers (50,51). However, the period of tea consumption referred to 5 years or less before the diagnosis or interview. Although the subjects might have been drinking tea for a long time, the duration and quantity of tea consumption was not assessed, so the effect of green tea could not be fully justified. Cohort studies, on the other hand, were based on information of tea consumption at the beginning of the study (53–55) and suggested that tea consumption increased with age (54,55). In particular, the Japanese study (53) with a long follow-up period of 14 years showed that people consuming green tea two to four times per day had a relative risk of 0.78 (95% CI = 0.6–1.0), when compared with those drinking once or less per day and after adjustment for potential confounders. But for subjects drinking green tea more than five times per day, the relative risk was only 0.79 (95% CI = 0.59–1.1), the non-significance result probably due to insufficient sample size at this high level of consumption (53).
Table 2. Epidemiology studies on tea and lung cancer

Country	Study design	Sample size	Tea type and highest consumption level	Reference period of intake	Smoking status of subjects	Results	
China (50)	Population-based case–control study	Cases: 649 Controls: 675	Green tea: >1500 g tea leaves per year	5 years before interview	Smokers and non-smokers	OR = 0.65; 95% CI = 0.45–0.93 for non-smokers OR = 0.94; 95% CI = 0.40–2.22 for smokers	
Canada (52)	Population-based case–control study	Cases: 582 Controls: 582	Type unspecified: >7 cups per day	2 years before study	Non-smokers	OR = 0.4; 95% CI = 02–0.7	
USA (51)	Population based case–control study	Cases: 161 Controls 483	Green tea: >1 cup per day	1 year before diagnosis or interview	Smokers and non-smokers	OR = 0.9; 95% CI = 0.5–1.6	
Japan (53)	Cohort study (1980–94)	Subjects: 38 540 Person-years at risk: unspecified Cases: 436	Green tea: >5 times per day	Unspecified; survey taken at start of study	Smokers and non-smokers	RR = 0.78; 95% CI = 0.6–1.0 for people drinking tea 2–4 times per day	
Japan (54)	Cohort study (1986–95)	Subjects: 8552 Person-years at risk: 71 248.5 Cases: 384	Green tea: >10 cups per day	Unspecified; survey taken at start of study	Smokers and non-smokers	Cancer in all sites RR = 0.68 for males; 95% CI = 0.39–1.21 RR = 0.57 for females; 95% CI = 0.33–0.98	
OR, odds ratio; RR, relative risk; CI, confidence interval.



In summary, there is only limited evidence from epidemiological studies that green tea is protective against lung cancer among smokers. Moreover, green tea exposure was generally taken to be within 5 years of interview or follow-up, which would have little impact on the incidence of lung cancer.

Conclusion
Our review suggests that regular intake of green tea at high levels (>3 cups per day) may offer protection against tobacco carcinogens for smokers, provided that the duration of green tea consumption is sufficiently long to cover the smoking period. For epidemiology studies, improvement in measuring green tea intake is required in order to confirm the chemopreventive effect of green tea observed in in vitro animal and human trials.
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